Mutagenetix > Incidental Mutation

Incidental Mutation 'R2513:Dlg5'

253688

Institutional Source

Beutler Lab

Gene Symbol

Dlg5

Ensembl Gene

ENSMUSG00000021782

Gene Name

discs large MAGUK scaffold protein 5

Synonyms

4933429D20Rik

MMRRC Submission

040419-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R2513 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

24184021-24295988 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 24214593 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Asparagine at position 663 (K663N)

Ref Sequence

ENSEMBL: ENSMUSP00000073367 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042009] [ENSMUST00000073687] [ENSMUST00000090398]

AlphaFold

E9Q9R9

Predicted Effect

probably damaging
Transcript: ENSMUST00000042009
AA Change: K332N

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000044852
Gene: ENSMUSG00000021782
AA Change: K332N

Domain	Start	End	E-Value	Type
coiled coil region	20	247	N/A	INTRINSIC
low complexity region	261	274	N/A	INTRINSIC
PDZ	279	356	2.02e-10	SMART
PDZ	364	447	9.5e-16	SMART
low complexity region	510	517	N/A	INTRINSIC
low complexity region	692	711	N/A	INTRINSIC
low complexity region	903	918	N/A	INTRINSIC
PDZ	1009	1080	2.1e-17	SMART
PDZ	1164	1236	2.97e-8	SMART
SH3	1250	1314	3.73e-7	SMART
low complexity region	1338	1358	N/A	INTRINSIC
GuKc	1375	1561	5.43e-53	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000073687
AA Change: K663N

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000073367
Gene: ENSMUSG00000021782
AA Change: K663N

Domain	Start	End	E-Value	Type
low complexity region	8	19	N/A	INTRINSIC
low complexity region	22	36	N/A	INTRINSIC
low complexity region	44	66	N/A	INTRINSIC
Pfam:Takusan	104	191	1.4e-27	PFAM
coiled coil region	308	578	N/A	INTRINSIC
low complexity region	592	605	N/A	INTRINSIC
PDZ	610	687	2.02e-10	SMART
PDZ	695	773	1.25e-15	SMART
low complexity region	836	843	N/A	INTRINSIC
low complexity region	1018	1037	N/A	INTRINSIC
low complexity region	1229	1244	N/A	INTRINSIC
PDZ	1335	1406	2.1e-17	SMART
PDZ	1490	1562	2.97e-8	SMART
SH3	1576	1640	3.73e-7	SMART
low complexity region	1664	1684	N/A	INTRINSIC
GuKc	1701	1887	5.43e-53	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000090398
AA Change: K686N

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000087879
Gene: ENSMUSG00000021782
AA Change: K686N

Domain	Start	End	E-Value	Type
low complexity region	8	19	N/A	INTRINSIC
low complexity region	22	36	N/A	INTRINSIC
low complexity region	44	66	N/A	INTRINSIC
low complexity region	109	123	N/A	INTRINSIC
Pfam:Takusan	128	213	6e-33	PFAM
coiled coil region	331	601	N/A	INTRINSIC
low complexity region	615	628	N/A	INTRINSIC
PDZ	633	710	2.02e-10	SMART
PDZ	718	796	1.25e-15	SMART
low complexity region	859	866	N/A	INTRINSIC
low complexity region	1041	1060	N/A	INTRINSIC
low complexity region	1252	1267	N/A	INTRINSIC
PDZ	1358	1429	2.1e-17	SMART
PDZ	1513	1585	2.97e-8	SMART
SH3	1599	1663	3.73e-7	SMART
low complexity region	1687	1707	N/A	INTRINSIC
GuKc	1724	1910	5.43e-53	SMART

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.4%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the family of discs large (DLG) homologs, a subset of the membrane-associated guanylate kinase (MAGUK) superfamily. The MAGUK proteins are composed of a catalytically inactive guanylate kinase domain, in addition to PDZ and SH3 domains, and are thought to function as scaffolding molecules at sites of cell-cell contact. The protein encoded by this gene localizes to the plasma membrane and cytoplasm, and interacts with components of adherens junctions and the cytoskeleton. It is proposed to function in the transmission of extracellular signals to the cytoskeleton and in the maintenance of epithelial cell structure. Alternative splice variants have been described but their biological nature has not been determined. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a null allele exhibit growth retardation, hydroencephaly, abnormal brain morphology, abnormal neurogenesis, kidney cysts, ureter defects, and abnormal kidney morphology. [provided by MGI curators]

Allele List at MGI

All alleles(19) : Targeted, other(1) Gene trapped(18)

Other mutations in this stock

Total: 90 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3425401B19Rik	T	C	14: 32,383,809 (GRCm39)	T719A	possibly damaging	Het
Abcc1	A	G	16: 14,290,873 (GRCm39)		probably null	Het
Aftph	C	T	11: 20,658,676 (GRCm39)		probably null	Het
Als2	C	A	1: 59,254,276 (GRCm39)	K360N	probably benign	Het
Atg2a	T	A	19: 6,308,076 (GRCm39)		probably null	Het
Axl	T	C	7: 25,486,941 (GRCm39)	D22G	probably benign	Het
Baiap2	G	A	11: 119,890,052 (GRCm39)	A438T	probably benign	Het
Bean1	G	C	8: 104,908,643 (GRCm39)	A7P	probably benign	Het
Birc6	C	G	17: 74,954,724 (GRCm39)	P3431R	probably damaging	Het
Camk1d	A	G	2: 5,719,047 (GRCm39)	M1T	probably null	Het
Carmil3	T	C	14: 55,741,295 (GRCm39)	Y1027H	probably damaging	Het
Ccdc6	G	A	10: 70,023,658 (GRCm39)		probably benign	Het
Cemip	C	A	7: 83,591,233 (GRCm39)	V1280L	probably benign	Het
Cfap58	C	A	19: 47,950,981 (GRCm39)	N447K	probably benign	Het
Chd3	A	G	11: 69,251,471 (GRCm39)	L520P	probably damaging	Het
Col1a2	T	A	6: 4,531,223 (GRCm39)		probably null	Het
Cpne7	A	C	8: 123,844,406 (GRCm39)		probably null	Het
Ctla4	T	C	1: 60,951,723 (GRCm39)	V84A	probably damaging	Het
Dap3	A	G	3: 88,835,565 (GRCm39)	V263A	probably benign	Het
Dhx57	T	A	17: 80,549,378 (GRCm39)		probably null	Het
Dsg3	C	A	18: 20,656,719 (GRCm39)	F196L	possibly damaging	Het
Eif2ak4	C	A	2: 118,257,064 (GRCm39)	D402E	probably damaging	Het
Fance	T	C	17: 28,537,068 (GRCm39)	V24A	probably benign	Het
Fasn	A	T	11: 120,705,574 (GRCm39)	L1149Q	probably damaging	Het
Fcgbpl1	C	G	7: 27,831,060 (GRCm39)	S91C	probably damaging	Het
Flg	T	A	3: 93,187,093 (GRCm39)	S182T	possibly damaging	Het
Gm128	T	A	3: 95,147,293 (GRCm39)	S334C	possibly damaging	Het
Gm3727	A	C	14: 7,264,561 (GRCm38)	H31Q	probably damaging	Het
Gm4841	T	G	18: 60,403,977 (GRCm39)	T39P	probably damaging	Het
Golgb1	G	T	16: 36,735,513 (GRCm39)	V1587L	possibly damaging	Het
Golm1	G	A	13: 59,790,072 (GRCm39)	P243S	probably benign	Het
Gstcd	C	A	3: 132,788,081 (GRCm39)	A206S	possibly damaging	Het
Gstcd	C	A	3: 132,788,082 (GRCm39)	K205N	possibly damaging	Het
Gtf3c1	A	G	7: 125,280,345 (GRCm39)	V355A	probably benign	Het
Hhatl	T	C	9: 121,618,236 (GRCm39)	D173G	probably benign	Het
Kcnu1	T	A	8: 26,395,994 (GRCm39)	C660S	probably benign	Het
Kctd8	T	C	5: 69,267,988 (GRCm39)	D374G	probably benign	Het
Kif21a	T	C	15: 90,878,594 (GRCm39)	I229V	possibly damaging	Het
L3mbtl1	A	T	2: 162,809,505 (GRCm39)	I702F	probably benign	Het
Lce1d	T	C	3: 92,593,066 (GRCm39)		probably benign	Het
Lrig1	C	T	6: 94,594,347 (GRCm39)		probably null	Het
Lrp12	A	T	15: 39,739,507 (GRCm39)	D563E	probably damaging	Het
Lrp2	C	T	2: 69,336,718 (GRCm39)		probably null	Het
Map3k5	G	A	10: 19,970,201 (GRCm39)	V703I	possibly damaging	Het
Mecr	A	G	4: 131,581,076 (GRCm39)	R110G	probably benign	Het
Mtrr	T	A	13: 68,715,092 (GRCm39)	K445*	probably null	Het
Nanos1	T	A	19: 60,744,990 (GRCm39)	L96Q	probably benign	Het
Or13a24	T	G	7: 140,154,069 (GRCm39)	M1R	probably null	Het
Or51t4	T	A	7: 102,598,700 (GRCm39)	F333I	probably benign	Het
Or6c214	T	A	10: 129,591,021 (GRCm39)	L99F	probably damaging	Het
Otog	T	C	7: 45,955,014 (GRCm39)		probably null	Het
Pcdhb3	G	T	18: 37,434,294 (GRCm39)	V87F	probably benign	Het
Pcdhb3	T	A	18: 37,434,292 (GRCm39)	L86*	probably null	Het
Pcdhb3	A	T	18: 37,434,293 (GRCm39)	L86F	probably damaging	Het
Pdgfd	A	G	9: 6,359,894 (GRCm39)	K322E	probably damaging	Het
Picalm	T	C	7: 89,846,217 (GRCm39)	S648P	probably damaging	Het
Pigr	T	C	1: 130,774,357 (GRCm39)	S446P	possibly damaging	Het
Pik3cb	A	C	9: 98,943,895 (GRCm39)	L636W	probably damaging	Het
Pla2g2f	A	T	4: 138,481,473 (GRCm39)	L92Q	probably damaging	Het
Plod3	G	C	5: 137,017,000 (GRCm39)	A50P	probably benign	Het
Prkar2b	C	T	12: 32,025,928 (GRCm39)	V191I	possibly damaging	Het
Prune1	C	A	3: 95,165,430 (GRCm39)	A281S	probably benign	Het
Ptges3l	A	T	11: 101,314,868 (GRCm39)	C42S	possibly damaging	Het
Reg3b	A	T	6: 78,348,802 (GRCm39)	I33L	probably benign	Het
Rel	A	G	11: 23,695,823 (GRCm39)	I188T	probably damaging	Het
Rif1	GCCACCA	GCCA	2: 52,000,336 (GRCm39)		probably benign	Het
Ripor1	G	T	8: 106,344,254 (GRCm39)	V463L	probably benign	Het
Rrm1	T	A	7: 102,109,896 (GRCm39)	D510E	probably damaging	Het
Setd7	A	G	3: 51,440,436 (GRCm39)	S202P	probably damaging	Het
Shank3	T	A	15: 89,432,889 (GRCm39)	D1211E	probably benign	Het
Slc30a1	A	T	1: 191,639,674 (GRCm39)	T186S	possibly damaging	Het
Slc4a4	G	A	5: 89,304,257 (GRCm39)	V523I	probably benign	Het
Slc9a2	T	A	1: 40,781,768 (GRCm39)		probably null	Het
Smg6	A	G	11: 74,820,502 (GRCm39)	T258A	probably damaging	Het
Sptlc1	C	T	13: 53,491,676 (GRCm39)	D408N	possibly damaging	Het
Tasor2	G	T	13: 3,632,150 (GRCm39)	L784I	possibly damaging	Het
Tbl3	T	C	17: 24,923,524 (GRCm39)		probably null	Het
Tdo2	T	C	3: 81,876,812 (GRCm39)	D139G	possibly damaging	Het
Ticam1	T	A	17: 56,578,612 (GRCm39)	H161L	possibly damaging	Het
Tnfaip3	T	A	10: 18,881,407 (GRCm39)	D293V	probably benign	Het
Trim72	G	A	7: 127,603,878 (GRCm39)	V75M	possibly damaging	Het
Trip11	T	A	12: 101,803,986 (GRCm39)	N1632I	possibly damaging	Het
Tspan18	A	T	2: 93,050,440 (GRCm39)	M61K	possibly damaging	Het
Tuba8	A	T	6: 121,202,932 (GRCm39)	E415V	probably damaging	Het
Ung	A	G	5: 114,275,253 (GRCm39)	H214R	probably benign	Het
Uqcrc1	T	C	9: 108,765,836 (GRCm39)	L17P	probably damaging	Het
Usp24	A	G	4: 106,236,602 (GRCm39)		probably null	Het
Vps39	A	C	2: 120,169,268 (GRCm39)	Y245D	probably damaging	Het
Whrn	T	A	4: 63,353,649 (GRCm39)	T373S	probably benign	Het
Zc2hc1a	C	T	3: 7,581,596 (GRCm39)		probably null	Het

Other mutations in Dlg5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00160:Dlg5	APN	14	24,241,229 (GRCm39)	missense	probably damaging	0.99
IGL00164:Dlg5	APN	14	24,208,532 (GRCm39)	missense	possibly damaging	0.89
IGL00767:Dlg5	APN	14	24,215,353 (GRCm39)	missense	probably damaging	1.00
IGL01284:Dlg5	APN	14	24,196,265 (GRCm39)	missense	probably damaging	1.00
IGL01328:Dlg5	APN	14	24,252,419 (GRCm39)	missense	probably damaging	0.98
IGL01532:Dlg5	APN	14	24,208,660 (GRCm39)	missense	probably benign
IGL01621:Dlg5	APN	14	24,198,289 (GRCm39)	missense	probably damaging	1.00
IGL01649:Dlg5	APN	14	24,188,759 (GRCm39)	missense	probably damaging	1.00
IGL01733:Dlg5	APN	14	24,220,517 (GRCm39)	missense	probably damaging	1.00
IGL02048:Dlg5	APN	14	24,222,271 (GRCm39)	missense	possibly damaging	0.87
IGL02103:Dlg5	APN	14	24,194,414 (GRCm39)	missense	probably damaging	1.00
IGL02138:Dlg5	APN	14	24,208,419 (GRCm39)	missense	probably benign
IGL02146:Dlg5	APN	14	24,252,429 (GRCm39)	missense	probably damaging	0.99
IGL02392:Dlg5	APN	14	24,200,277 (GRCm39)	missense	probably damaging	1.00
IGL02427:Dlg5	APN	14	24,216,275 (GRCm39)	missense	probably damaging	1.00
IGL02643:Dlg5	APN	14	24,241,250 (GRCm39)	missense	probably damaging	1.00
IGL02649:Dlg5	APN	14	24,196,319 (GRCm39)	missense	probably damaging	0.96
IGL02933:Dlg5	APN	14	24,208,567 (GRCm39)	missense	probably benign	0.06
IGL02965:Dlg5	APN	14	24,222,091 (GRCm39)	missense	probably damaging	1.00
IGL02988:Dlg5	APN	14	24,216,323 (GRCm39)	missense	probably damaging	1.00
IGL03351:Dlg5	APN	14	24,220,522 (GRCm39)	missense	probably benign	0.03
legerdemain	UTSW	14	24,214,615 (GRCm39)	missense	probably damaging	1.00
R0123:Dlg5	UTSW	14	24,197,274 (GRCm39)	missense	probably benign
R0131:Dlg5	UTSW	14	24,188,717 (GRCm39)	missense	probably damaging	1.00
R0709:Dlg5	UTSW	14	24,196,323 (GRCm39)	missense	probably damaging	1.00
R0920:Dlg5	UTSW	14	24,226,465 (GRCm39)	missense	probably damaging	1.00
R0924:Dlg5	UTSW	14	24,185,645 (GRCm39)	missense	probably damaging	1.00
R0930:Dlg5	UTSW	14	24,185,645 (GRCm39)	missense	probably damaging	1.00
R0981:Dlg5	UTSW	14	24,204,699 (GRCm39)	missense	probably damaging	1.00
R1402:Dlg5	UTSW	14	24,226,676 (GRCm39)	missense	probably benign	0.06
R1402:Dlg5	UTSW	14	24,226,676 (GRCm39)	missense	probably benign	0.06
R1438:Dlg5	UTSW	14	24,204,673 (GRCm39)	missense	possibly damaging	0.94
R1449:Dlg5	UTSW	14	24,185,711 (GRCm39)	missense	possibly damaging	0.82
R1465:Dlg5	UTSW	14	24,204,764 (GRCm39)	splice site	probably null
R1465:Dlg5	UTSW	14	24,204,764 (GRCm39)	splice site	probably null
R1543:Dlg5	UTSW	14	24,194,516 (GRCm39)	missense	probably damaging	1.00
R1824:Dlg5	UTSW	14	24,199,512 (GRCm39)	missense	probably benign	0.28
R1899:Dlg5	UTSW	14	24,198,368 (GRCm39)	missense	probably damaging	1.00
R1920:Dlg5	UTSW	14	24,226,639 (GRCm39)	missense	probably damaging	1.00
R1921:Dlg5	UTSW	14	24,226,639 (GRCm39)	missense	probably damaging	1.00
R1951:Dlg5	UTSW	14	24,206,537 (GRCm39)	splice site	probably benign
R1968:Dlg5	UTSW	14	24,214,187 (GRCm39)	nonsense	probably null
R2049:Dlg5	UTSW	14	24,204,715 (GRCm39)	missense	probably damaging	1.00
R2070:Dlg5	UTSW	14	24,186,703 (GRCm39)	missense	probably damaging	1.00
R2117:Dlg5	UTSW	14	24,227,826 (GRCm39)	nonsense	probably null
R2139:Dlg5	UTSW	14	24,220,612 (GRCm39)	missense	probably damaging	1.00
R2153:Dlg5	UTSW	14	24,187,225 (GRCm39)	missense	probably damaging	1.00
R2283:Dlg5	UTSW	14	24,208,731 (GRCm39)	missense	probably benign	0.00
R2293:Dlg5	UTSW	14	24,208,180 (GRCm39)	missense	probably benign
R2356:Dlg5	UTSW	14	24,220,496 (GRCm39)	critical splice donor site	probably null
R2362:Dlg5	UTSW	14	24,208,755 (GRCm39)	missense	probably benign	0.04
R3084:Dlg5	UTSW	14	24,216,258 (GRCm39)	missense	probably damaging	1.00
R3086:Dlg5	UTSW	14	24,216,258 (GRCm39)	missense	probably damaging	1.00
R3750:Dlg5	UTSW	14	24,215,328 (GRCm39)	missense	probably damaging	1.00
R3780:Dlg5	UTSW	14	24,240,378 (GRCm39)	unclassified	probably benign
R3782:Dlg5	UTSW	14	24,240,378 (GRCm39)	unclassified	probably benign
R3828:Dlg5	UTSW	14	24,196,226 (GRCm39)	missense	probably damaging	0.99
R4079:Dlg5	UTSW	14	24,198,328 (GRCm39)	missense	possibly damaging	0.94
R4393:Dlg5	UTSW	14	24,228,057 (GRCm39)	critical splice acceptor site	probably null
R4615:Dlg5	UTSW	14	24,208,236 (GRCm39)	missense	probably damaging	1.00
R4664:Dlg5	UTSW	14	24,187,249 (GRCm39)	missense	possibly damaging	0.90
R4712:Dlg5	UTSW	14	24,228,051 (GRCm39)	missense	possibly damaging	0.94
R4796:Dlg5	UTSW	14	24,194,451 (GRCm39)	missense	probably damaging	1.00
R4801:Dlg5	UTSW	14	24,204,757 (GRCm39)	missense	probably damaging	1.00
R4802:Dlg5	UTSW	14	24,204,757 (GRCm39)	missense	probably damaging	1.00
R4946:Dlg5	UTSW	14	24,204,429 (GRCm39)	missense	probably damaging	0.99
R5022:Dlg5	UTSW	14	24,186,690 (GRCm39)	missense	probably damaging	1.00
R5023:Dlg5	UTSW	14	24,186,690 (GRCm39)	missense	probably damaging	1.00
R5057:Dlg5	UTSW	14	24,186,690 (GRCm39)	missense	probably damaging	1.00
R5234:Dlg5	UTSW	14	24,242,930 (GRCm39)	missense	probably damaging	0.98
R5561:Dlg5	UTSW	14	24,227,860 (GRCm39)	missense	probably benign	0.03
R5567:Dlg5	UTSW	14	24,242,981 (GRCm39)	nonsense	probably null
R5570:Dlg5	UTSW	14	24,242,981 (GRCm39)	nonsense	probably null
R5640:Dlg5	UTSW	14	24,220,529 (GRCm39)	missense	probably damaging	1.00
R5646:Dlg5	UTSW	14	24,208,767 (GRCm39)	missense	probably damaging	1.00
R5711:Dlg5	UTSW	14	24,200,716 (GRCm39)	missense	probably damaging	1.00
R5810:Dlg5	UTSW	14	24,196,322 (GRCm39)	missense	probably damaging	0.99
R5900:Dlg5	UTSW	14	24,199,515 (GRCm39)	missense	probably damaging	1.00
R5964:Dlg5	UTSW	14	24,214,157 (GRCm39)	missense	probably benign
R6190:Dlg5	UTSW	14	24,240,506 (GRCm39)	missense	probably damaging	0.99
R6240:Dlg5	UTSW	14	24,199,596 (GRCm39)	splice site	probably null
R6276:Dlg5	UTSW	14	24,214,636 (GRCm39)	missense	probably damaging	1.00
R6339:Dlg5	UTSW	14	24,208,128 (GRCm39)	missense	probably damaging	1.00
R6508:Dlg5	UTSW	14	24,188,774 (GRCm39)	missense	probably benign	0.45
R6527:Dlg5	UTSW	14	24,240,516 (GRCm39)	missense	possibly damaging	0.73
R6593:Dlg5	UTSW	14	24,200,720 (GRCm39)	missense	probably benign	0.01
R6687:Dlg5	UTSW	14	24,240,441 (GRCm39)	missense	probably damaging	1.00
R6965:Dlg5	UTSW	14	24,199,498 (GRCm39)	missense	probably damaging	1.00
R7051:Dlg5	UTSW	14	24,196,263 (GRCm39)	missense	possibly damaging	0.93
R7075:Dlg5	UTSW	14	24,227,865 (GRCm39)	missense	possibly damaging	0.49
R7149:Dlg5	UTSW	14	24,240,492 (GRCm39)	missense	probably benign	0.00
R7182:Dlg5	UTSW	14	24,294,924 (GRCm39)	missense
R7203:Dlg5	UTSW	14	24,188,723 (GRCm39)	missense	probably damaging	1.00
R7216:Dlg5	UTSW	14	24,186,706 (GRCm39)	nonsense	probably null
R7359:Dlg5	UTSW	14	24,214,615 (GRCm39)	missense	probably damaging	1.00
R7466:Dlg5	UTSW	14	24,295,280 (GRCm39)	missense	probably damaging	1.00
R7485:Dlg5	UTSW	14	24,227,907 (GRCm39)	missense	probably damaging	0.98
R7485:Dlg5	UTSW	14	24,198,390 (GRCm39)	missense	probably benign
R7629:Dlg5	UTSW	14	24,295,280 (GRCm39)	missense	probably damaging	1.00
R7666:Dlg5	UTSW	14	24,207,867 (GRCm39)	missense	probably damaging	1.00
R7804:Dlg5	UTSW	14	24,215,388 (GRCm39)	missense	possibly damaging	0.46
R7861:Dlg5	UTSW	14	24,295,280 (GRCm39)	missense	probably damaging	1.00
R7862:Dlg5	UTSW	14	24,295,280 (GRCm39)	missense	probably damaging	1.00
R7864:Dlg5	UTSW	14	24,295,280 (GRCm39)	missense	probably damaging	1.00
R7874:Dlg5	UTSW	14	24,185,687 (GRCm39)	missense	probably damaging	1.00
R7913:Dlg5	UTSW	14	24,187,192 (GRCm39)	splice site	probably null
R7981:Dlg5	UTSW	14	24,208,213 (GRCm39)	missense	probably benign
R8147:Dlg5	UTSW	14	24,208,395 (GRCm39)	missense	probably benign	0.07
R8204:Dlg5	UTSW	14	24,210,320 (GRCm39)	missense	probably damaging	1.00
R8206:Dlg5	UTSW	14	24,210,336 (GRCm39)	missense	possibly damaging	0.62
R8287:Dlg5	UTSW	14	24,214,453 (GRCm39)	missense	probably benign	0.40
R8296:Dlg5	UTSW	14	24,198,328 (GRCm39)	missense	possibly damaging	0.94
R8317:Dlg5	UTSW	14	24,241,298 (GRCm39)	missense	probably damaging	0.98
R8327:Dlg5	UTSW	14	24,196,388 (GRCm39)	missense	probably damaging	0.99
R8352:Dlg5	UTSW	14	24,241,261 (GRCm39)	missense	probably damaging	1.00
R8353:Dlg5	UTSW	14	24,208,213 (GRCm39)	missense	probably benign
R8409:Dlg5	UTSW	14	24,226,546 (GRCm39)	missense	probably damaging	1.00
R8452:Dlg5	UTSW	14	24,241,261 (GRCm39)	missense	probably damaging	1.00
R8453:Dlg5	UTSW	14	24,208,213 (GRCm39)	missense	probably benign
R8540:Dlg5	UTSW	14	24,208,767 (GRCm39)	missense	probably damaging	1.00
R8701:Dlg5	UTSW	14	24,226,768 (GRCm39)	missense	probably benign	0.04
R8925:Dlg5	UTSW	14	24,206,547 (GRCm39)	missense
R8927:Dlg5	UTSW	14	24,206,547 (GRCm39)	missense
R9025:Dlg5	UTSW	14	24,199,546 (GRCm39)	missense	probably benign	0.00
R9102:Dlg5	UTSW	14	24,199,567 (GRCm39)	missense	probably damaging	1.00
R9138:Dlg5	UTSW	14	24,295,376 (GRCm39)	missense	probably damaging	0.98
R9165:Dlg5	UTSW	14	24,196,309 (GRCm39)	missense	probably damaging	1.00
R9250:Dlg5	UTSW	14	24,240,543 (GRCm39)	missense	probably benign	0.07
R9267:Dlg5	UTSW	14	24,204,745 (GRCm39)	missense	probably damaging	1.00
R9269:Dlg5	UTSW	14	24,242,881 (GRCm39)	missense	probably damaging	0.99
R9291:Dlg5	UTSW	14	24,241,229 (GRCm39)	missense	probably damaging	0.99
R9387:Dlg5	UTSW	14	24,197,168 (GRCm39)	missense	probably damaging	0.99
R9729:Dlg5	UTSW	14	24,204,681 (GRCm39)	missense	probably benign	0.00
RF005:Dlg5	UTSW	14	24,208,561 (GRCm39)	nonsense	probably null
YA93:Dlg5	UTSW	14	24,205,201 (GRCm39)	unclassified	probably benign
Z1088:Dlg5	UTSW	14	24,208,162 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAAACTGTCCCCTCTAGCAG -3'
(R):5'- CCAGCATCCGTGAGTTTTATAAC -3'

Sequencing Primer
(F):5'- AGAGGGGATATCTGCCTCAC -3'
(R):5'- TATAACCATGCACTCTGGAGGCTG -3'

Posted On

2014-12-04