Mutagenetix > Incidental Mutation

Incidental Mutation 'R2763:Gstm6'

254138

Institutional Source

Beutler Lab

Gene Symbol

Gstm6

Ensembl Gene

ENSMUSG00000068762

Gene Name

glutathione S-transferase, mu 6

Synonyms

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2763 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

107846163-107851065 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 107848358 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Methionine at position 173 (T173M)

Ref Sequence

ENSEMBL: ENSMUSP00000102296 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000106681] [ENSMUST00000106683] [ENSMUST00000106684] [ENSMUST00000106685] [ENSMUST00000155926]

AlphaFold

O35660

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000106680

SMART Domains

Protein: ENSMUSP00000102291
Gene: ENSMUSG00000068762

Domain	Start	End	E-Value	Type
Pfam:GST_N	3	77	6.2e-13	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000106681
AA Change: T139M

PolyPhen 2 Score 0.265 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000102292
Gene: ENSMUSG00000068762
AA Change: T139M

Domain	Start	End	E-Value	Type
Pfam:GST_N	1	48	9.4e-12	PFAM
Pfam:GST_C_3	7	154	7.6e-11	PFAM
Pfam:GST_C	70	154	3.6e-20	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000106683
AA Change: T78M

PolyPhen 2 Score 0.522 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000102294
Gene: ENSMUSG00000068762
AA Change: T78M

Domain	Start	End	E-Value	Type
Pfam:GST_C_3	4	95	6.3e-11	PFAM
Pfam:GST_C	6	97	2.1e-18	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000106684
AA Change: T187M

PolyPhen 2 Score 0.265 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000102295
Gene: ENSMUSG00000068762
AA Change: T187M

Domain	Start	End	E-Value	Type
Pfam:GST_N	3	82	2e-22	PFAM
Pfam:GST_C_3	41	204	4.1e-12	PFAM
Pfam:GST_C	104	205	5.9e-18	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000106685
AA Change: T173M

PolyPhen 2 Score 0.648 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000102296
Gene: ENSMUSG00000068762
AA Change: T173M

Domain	Start	End	E-Value	Type
Pfam:GST_N	3	82	7.9e-23	PFAM
Pfam:GST_C	104	192	8.4e-20	PFAM
Pfam:GST_C_3	113	190	6.8e-10	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000155926

SMART Domains

Protein: ENSMUSP00000139500
Gene: ENSMUSG00000068762

Domain	Start	End	E-Value	Type
Pfam:GST_N	1	48	1.9e-10	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 23 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A4galt	T	A	15: 83,111,871 (GRCm39)	D304V	probably benign	Het
Ablim3	T	A	18: 61,946,615 (GRCm39)	K516*	probably null	Het
Akap11	A	G	14: 78,756,332 (GRCm39)	F22S	probably damaging	Het
Alg11	C	A	8: 22,558,095 (GRCm39)	A469E	probably benign	Het
Ano7	A	G	1: 93,326,908 (GRCm39)		probably null	Het
Apol9a	T	C	15: 77,288,617 (GRCm39)	E250G	probably benign	Het
Calcrl	T	C	2: 84,200,847 (GRCm39)	R66G	probably damaging	Het
Camta2	G	A	11: 70,573,356 (GRCm39)	Q187*	probably null	Het
Cd177	G	A	7: 24,457,462 (GRCm39)	A193V	probably benign	Het
Cdk5rap2	A	G	4: 70,199,508 (GRCm39)	V872A	probably benign	Het
Cebpz	A	G	17: 79,243,358 (GRCm39)	S99P	probably benign	Het
Cmtr1	G	A	17: 29,899,602 (GRCm39)	E632K	possibly damaging	Het
Dysf	A	T	6: 84,083,914 (GRCm39)	H782L	probably benign	Het
Fem1a	A	G	17: 56,564,537 (GRCm39)	E210G	probably benign	Het
Golga3	T	C	5: 110,352,761 (GRCm39)	I884T	possibly damaging	Het
Pcdhgb8	T	A	18: 37,895,315 (GRCm39)	N128K	probably damaging	Het
Phkg2	A	G	7: 127,179,005 (GRCm39)	E139G	probably benign	Het
Sdk1	T	A	5: 142,070,306 (GRCm39)	V1157E	possibly damaging	Het
Septin9	C	T	11: 117,217,327 (GRCm39)	T6I	probably benign	Het
Shkbp1	T	A	7: 27,046,454 (GRCm39)	M437L	probably benign	Het
Svep1	A	G	4: 58,084,061 (GRCm39)	C1904R	possibly damaging	Het
Ulk1	C	T	5: 110,937,223 (GRCm39)	R691Q	probably benign	Het
Zfp646	T	A	7: 127,479,210 (GRCm39)	C242*	probably null	Het

Other mutations in Gstm6

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01321:Gstm6	APN	3	107,848,379 (GRCm39)	missense	probably benign	0.15
IGL02100:Gstm6	APN	3	107,849,653 (GRCm39)	missense	probably benign	0.20
IGL02685:Gstm6	APN	3	107,848,507 (GRCm39)	missense	probably benign	0.25
R1221:Gstm6	UTSW	3	107,848,418 (GRCm39)	missense	probably damaging	1.00
R2428:Gstm6	UTSW	3	107,850,922 (GRCm39)	missense	possibly damaging	0.82
R6178:Gstm6	UTSW	3	107,848,397 (GRCm39)	missense	probably benign	0.01
R6545:Gstm6	UTSW	3	107,849,681 (GRCm39)	missense	probably damaging	1.00
R6730:Gstm6	UTSW	3	107,850,041 (GRCm39)	nonsense	probably null
R8240:Gstm6	UTSW	3	107,849,453 (GRCm39)	missense	probably damaging	0.99
R9354:Gstm6	UTSW	3	107,850,018 (GRCm39)	missense	probably damaging	1.00
X0018:Gstm6	UTSW	3	107,850,063 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATCGTGACAGCTAATTCTACCCAG -3'
(R):5'- CATGGTTTGCAGGGGACAAG -3'

Sequencing Primer
(F):5'- TTGACTCCGCAGATCTAGAAGGC -3'
(R):5'- TTTGCAGGGGACAAGGTAGAG -3'

Posted On

2014-12-04