Mutagenetix > Incidental Mutation

Incidental Mutation 'R2519:Wnk2'

254246

Institutional Source

Beutler Lab

Gene Symbol

Wnk2

Ensembl Gene

ENSMUSG00000037989

Gene Name

WNK lysine deficient protein kinase 2

Synonyms

X83337, ESTM15, 1810073P09Rik

MMRRC Submission

040423-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.316) question?

Stock #

R2519 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

49189779-49301490 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 49224505 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Glutamic Acid at position 1019 (K1019E)

Ref Sequence

ENSEMBL: ENSMUSP00000105723 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000035538] [ENSMUST00000049265] [ENSMUST00000091623] [ENSMUST00000110096] [ENSMUST00000110097] [ENSMUST00000159559] [ENSMUST00000160087] [ENSMUST00000162403] [ENSMUST00000162581]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000035538
AA Change: K1117E

PolyPhen 2 Score 0.223 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000047231
Gene: ENSMUSG00000037989
AA Change: K1117E

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.5e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.6e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	6.72e-6	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
low complexity region	2033	2053	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000049265
AA Change: K1117E

PolyPhen 2 Score 0.223 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000049327
Gene: ENSMUSG00000037989
AA Change: K1117E

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
S_TKc	195	453	3.3e-19	SMART
Pfam:OSR1_C	474	511	5.6e-19	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	9.51e-6	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
internal_repeat_2	1611	1703	2.12e-5	PROSPERO
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
internal_repeat_2	1923	2023	2.12e-5	PROSPERO
low complexity region	2069	2089	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000091623
AA Change: K1117E

PolyPhen 2 Score 0.223 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000089212
Gene: ENSMUSG00000037989
AA Change: K1117E

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	1.6e-41	PFAM
Pfam:Pkinase	195	453	1e-54	PFAM
Pfam:OSR1_C	474	511	4e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	1.52e-5	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
internal_repeat_2	1611	1713	7.41e-5	PROSPERO
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
internal_repeat_2	1923	2027	7.41e-5	PROSPERO
low complexity region	2117	2137	N/A	INTRINSIC
low complexity region	2191	2202	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000110096
AA Change: K1019E

PolyPhen 2 Score 0.989 (Sensitivity: 0.72; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000105723
Gene: ENSMUSG00000037989
AA Change: K1019E

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.6e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.6e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	1015	1038	N/A	INTRINSIC
internal_repeat_1	1064	1087	2.89e-5	PROSPERO
low complexity region	1177	1194	N/A	INTRINSIC
low complexity region	1219	1255	N/A	INTRINSIC
low complexity region	1331	1343	N/A	INTRINSIC
low complexity region	1351	1359	N/A	INTRINSIC
low complexity region	1366	1391	N/A	INTRINSIC
low complexity region	1489	1498	N/A	INTRINSIC
low complexity region	1721	1732	N/A	INTRINSIC
coiled coil region	1737	1768	N/A	INTRINSIC
low complexity region	2018	2038	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000110097
AA Change: K1117E

PolyPhen 2 Score 0.934 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000105724
Gene: ENSMUSG00000037989
AA Change: K1117E

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.7e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.8e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	1.16e-5	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
internal_repeat_2	1611	1713	5.74e-5	PROSPERO
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
internal_repeat_2	1923	2027	5.74e-5	PROSPERO
low complexity region	2117	2137	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000159559
AA Change: K1105E

PolyPhen 2 Score 0.505 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000123915
Gene: ENSMUSG00000037989
AA Change: K1105E

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.7e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.8e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	736	N/A	INTRINSIC
low complexity region	759	791	N/A	INTRINSIC
low complexity region	809	819	N/A	INTRINSIC
low complexity region	828	842	N/A	INTRINSIC
low complexity region	863	878	N/A	INTRINSIC
low complexity region	926	942	N/A	INTRINSIC
low complexity region	971	1002	N/A	INTRINSIC
low complexity region	1009	1036	N/A	INTRINSIC
low complexity region	1101	1124	N/A	INTRINSIC
internal_repeat_1	1150	1173	7.19e-6	PROSPERO
low complexity region	1263	1280	N/A	INTRINSIC
low complexity region	1305	1341	N/A	INTRINSIC
low complexity region	1417	1429	N/A	INTRINSIC
low complexity region	1437	1445	N/A	INTRINSIC
low complexity region	1452	1477	N/A	INTRINSIC
low complexity region	1575	1585	N/A	INTRINSIC
internal_repeat_2	1599	1701	3.66e-5	PROSPERO
low complexity region	1808	1819	N/A	INTRINSIC
coiled coil region	1824	1855	N/A	INTRINSIC
internal_repeat_2	1911	2015	3.66e-5	PROSPERO
low complexity region	2105	2125	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000159633

Predicted Effect

possibly damaging
Transcript: ENSMUST00000160087
AA Change: K259E

PolyPhen 2 Score 0.890 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000124614
Gene: ENSMUSG00000037989
AA Change: K259E

Domain	Start	End	E-Value	Type
low complexity region	61	71	N/A	INTRINSIC
low complexity region	80	94	N/A	INTRINSIC
low complexity region	115	130	N/A	INTRINSIC
low complexity region	178	194	N/A	INTRINSIC
low complexity region	255	278	N/A	INTRINSIC
low complexity region	417	434	N/A	INTRINSIC
low complexity region	459	495	N/A	INTRINSIC
low complexity region	571	583	N/A	INTRINSIC
low complexity region	591	599	N/A	INTRINSIC
low complexity region	606	631	N/A	INTRINSIC
low complexity region	729	738	N/A	INTRINSIC
low complexity region	961	972	N/A	INTRINSIC
coiled coil region	977	1008	N/A	INTRINSIC
low complexity region	1258	1278	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000162403
AA Change: K1007E

PolyPhen 2 Score 0.408 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000125539
Gene: ENSMUSG00000037989
AA Change: K1007E

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.3e-42	PFAM
Pfam:Pkinase	195	453	1.6e-56	PFAM
Pfam:OSR1_C	474	511	5.3e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	736	N/A	INTRINSIC
low complexity region	759	791	N/A	INTRINSIC
low complexity region	809	819	N/A	INTRINSIC
low complexity region	828	842	N/A	INTRINSIC
low complexity region	863	878	N/A	INTRINSIC
low complexity region	926	942	N/A	INTRINSIC
low complexity region	1003	1026	N/A	INTRINSIC
internal_repeat_1	1052	1075	1.05e-5	PROSPERO
low complexity region	1165	1182	N/A	INTRINSIC
low complexity region	1207	1243	N/A	INTRINSIC
low complexity region	1319	1331	N/A	INTRINSIC
low complexity region	1339	1347	N/A	INTRINSIC
low complexity region	1354	1379	N/A	INTRINSIC
low complexity region	1477	1487	N/A	INTRINSIC
internal_repeat_2	1501	1593	2.32e-5	PROSPERO
low complexity region	1710	1721	N/A	INTRINSIC
coiled coil region	1726	1757	N/A	INTRINSIC
internal_repeat_2	1813	1913	2.32e-5	PROSPERO
low complexity region	1959	1979	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000162581
AA Change: K1117E

PolyPhen 2 Score 0.223 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000125448
Gene: ENSMUSG00000037989
AA Change: K1117E

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.5e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.6e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	6.72e-6	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
low complexity region	2033	2053	N/A	INTRINSIC

Meta Mutation Damage Score

0.0894

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.4%

Validation Efficiency

99% (72/73)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a cytoplasmic serine-threonine kinase that belongs to the protein kinase superfamily. The protein plays an important role in the regulation of electrolyte homeostasis, cell signaling survival, and proliferation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Allele List at MGI

Other mutations in this stock

Total: 77 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933408J17Rik	G	A	10: 93,425,450 (GRCm39)		probably benign	Het
Abtb3	T	A	10: 85,487,475 (GRCm39)	V981D	probably damaging	Het
Actn1	A	T	12: 80,239,163 (GRCm39)	H247Q	probably damaging	Het
Adgre1	T	C	17: 57,717,956 (GRCm39)	C323R	probably damaging	Het
Adgrf2	T	A	17: 43,021,298 (GRCm39)	I509F	probably damaging	Het
Aknad1	A	T	3: 108,663,784 (GRCm39)	T331S	probably damaging	Het
Aldh1a3	T	C	7: 66,072,047 (GRCm39)	D39G	probably benign	Het
Alms1	C	A	6: 85,644,945 (GRCm39)		probably benign	Het
Ankhd1	T	G	18: 36,711,596 (GRCm39)		probably null	Het
Arfgef2	T	C	2: 166,723,164 (GRCm39)	S1535P	probably benign	Het
Bicc1	T	C	10: 70,766,474 (GRCm39)	E916G	probably damaging	Het
Birc2	A	T	9: 7,821,180 (GRCm39)	D381E	possibly damaging	Het
Carnmt1	A	G	19: 18,671,075 (GRCm39)	I316V	probably benign	Het
Cdk2ap1rt	A	G	11: 48,716,950 (GRCm39)	I76T	probably damaging	Het
Chd6	T	A	2: 160,871,796 (GRCm39)	Y213F	possibly damaging	Het
Coq7	A	T	7: 118,109,371 (GRCm39)	W226R	unknown	Het
Cyp2d9	G	A	15: 82,338,719 (GRCm39)		probably null	Het
Ddx42	T	A	11: 106,136,155 (GRCm39)	N635K	probably damaging	Het
Dmtf1	T	C	5: 9,179,323 (GRCm39)	T292A	possibly damaging	Het
Dnajb8	T	C	6: 88,199,857 (GRCm39)	V131A	probably benign	Het
Dock6	T	C	9: 21,727,629 (GRCm39)	E1367G	possibly damaging	Het
Dvl1	T	A	4: 155,940,000 (GRCm39)	Y377*	probably null	Het
Eif4g3	T	A	4: 137,824,629 (GRCm39)	F278Y	probably benign	Het
Fancg	A	T	4: 43,008,787 (GRCm39)	L150H	probably damaging	Het
Fastkd5	T	C	2: 130,458,114 (GRCm39)	T159A	possibly damaging	Het
Fkrp	G	T	7: 16,544,877 (GRCm39)	Y328*	probably null	Het
Fmo3	A	T	1: 162,785,874 (GRCm39)	V372D	probably damaging	Het
Gsta5	T	G	9: 78,211,721 (GRCm39)	L161R	probably damaging	Het
Gtf2h4	C	A	17: 35,981,801 (GRCm39)	G143W	probably damaging	Het
Hivep2	C	A	10: 14,004,713 (GRCm39)	T437K	probably benign	Het
Hmcn1	A	T	1: 150,649,571 (GRCm39)	Y638*	probably null	Het
Ighv11-2	T	A	12: 114,011,912 (GRCm39)	Q101L	probably damaging	Het
Lgalsl2	A	T	7: 5,362,833 (GRCm39)	I155F	probably damaging	Het
Lipf	G	T	19: 33,942,925 (GRCm39)	V78L	probably damaging	Het
Magi3	T	C	3: 103,923,081 (GRCm39)	E1212G	probably benign	Het
Mfap5	T	C	6: 122,502,948 (GRCm39)	S75P	probably damaging	Het
Mn1	C	A	5: 111,566,418 (GRCm39)	H129Q	possibly damaging	Het
Morc3	A	G	16: 93,659,427 (GRCm39)		probably null	Het
Mrc2	G	A	11: 105,239,257 (GRCm39)		probably null	Het
Myo5c	A	G	9: 75,157,718 (GRCm39)	I224V	probably damaging	Het
Nup58	T	C	14: 60,460,808 (GRCm39)	T486A	probably benign	Het
Or5m13b	A	G	2: 85,753,951 (GRCm39)	Y113C	probably damaging	Het
Or8b46	T	A	9: 38,450,281 (GRCm39)	V30D	probably damaging	Het
Parp14	A	T	16: 35,678,573 (GRCm39)	L465Q	possibly damaging	Het
Pcare	C	T	17: 72,058,642 (GRCm39)	S345N	probably damaging	Het
Plcd3	A	T	11: 102,971,226 (GRCm39)	I110N	possibly damaging	Het
Prx	A	G	7: 27,217,668 (GRCm39)	E862G	probably benign	Het
Rad50	G	A	11: 53,598,012 (GRCm39)		probably benign	Het
Rbm15	A	G	3: 107,238,149 (GRCm39)	S750P	probably benign	Het
Reln	C	A	5: 22,549,367 (GRCm39)	A14S	unknown	Het
Rph3a	T	C	5: 121,092,485 (GRCm39)	Y372C	probably damaging	Het
Serpine2	T	A	1: 79,777,256 (GRCm39)	H187L	possibly damaging	Het
Slc11a2	T	C	15: 100,299,204 (GRCm39)	D122G	probably damaging	Het
Slc25a32	A	T	15: 38,959,450 (GRCm39)	V289E	probably damaging	Het
Slc25a46	A	T	18: 31,735,814 (GRCm39)	S142T	probably benign	Het
Srm	A	G	4: 148,675,961 (GRCm39)		probably null	Het
Srsf5	A	G	12: 80,995,870 (GRCm39)	D123G	probably damaging	Het
Stab1	A	T	14: 30,876,829 (GRCm39)	C832S	probably damaging	Het
Stab2	A	C	10: 86,770,704 (GRCm39)		probably benign	Het
Suds3	T	C	5: 117,233,018 (GRCm39)	N282S	probably damaging	Het
Taar9	A	T	10: 23,985,152 (GRCm39)	V94E	probably damaging	Het
Taf2	C	A	15: 54,915,643 (GRCm39)	A428S	probably benign	Het
Tbk1	G	T	10: 121,393,164 (GRCm39)	T462K	probably benign	Het
Tcaf2	T	G	6: 42,606,365 (GRCm39)	I530L	possibly damaging	Het
Tigd4	A	G	3: 84,501,221 (GRCm39)	Y46C	probably damaging	Het
Topors	G	T	4: 40,261,714 (GRCm39)	Y523*	probably null	Het
Tpte	T	C	8: 22,823,176 (GRCm39)		probably benign	Het
Trpv5	T	A	6: 41,651,284 (GRCm39)	Q254L	probably damaging	Het
Trpv6	G	A	6: 41,601,550 (GRCm39)	Q457*	probably null	Het
Ush2a	A	T	1: 187,999,304 (GRCm39)	M205L	probably benign	Het
Vmn1r90	A	T	7: 14,295,643 (GRCm39)	Y152N	probably damaging	Het
Vmn2r124	T	A	17: 18,294,280 (GRCm39)	V789D	probably damaging	Het
Vmn2r99	T	C	17: 19,598,970 (GRCm39)	I218T	probably damaging	Het
Vstm2b	T	C	7: 40,552,299 (GRCm39)	V248A	probably benign	Het
Zfhx4	C	T	3: 5,468,418 (GRCm39)	P2859S	probably benign	Het
Zfp229	T	A	17: 21,964,568 (GRCm39)	F266Y	possibly damaging	Het
Zfp616	T	A	11: 73,975,094 (GRCm39)	C454*	probably null	Het

Other mutations in Wnk2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01518:Wnk2	APN	13	49,221,668 (GRCm39)	missense	possibly damaging	0.89
IGL01575:Wnk2	APN	13	49,300,152 (GRCm39)	missense	probably damaging	1.00
IGL01601:Wnk2	APN	13	49,230,038 (GRCm39)	missense	probably damaging	1.00
IGL01775:Wnk2	APN	13	49,224,586 (GRCm39)	missense	probably damaging	1.00
IGL02013:Wnk2	APN	13	49,235,510 (GRCm39)	missense	possibly damaging	0.46
IGL02016:Wnk2	APN	13	49,210,381 (GRCm39)	missense	probably damaging	1.00
IGL02167:Wnk2	APN	13	49,224,601 (GRCm39)	critical splice acceptor site	probably null
IGL02174:Wnk2	APN	13	49,210,643 (GRCm39)	missense	probably damaging	1.00
IGL02210:Wnk2	APN	13	49,244,345 (GRCm39)	missense	probably damaging	0.98
IGL02228:Wnk2	APN	13	49,210,416 (GRCm39)	missense	probably damaging	1.00
IGL02282:Wnk2	APN	13	49,221,601 (GRCm39)	missense	probably damaging	1.00
IGL02319:Wnk2	APN	13	49,214,914 (GRCm39)	missense	possibly damaging	0.73
IGL02394:Wnk2	APN	13	49,235,375 (GRCm39)	splice site	probably null
IGL02624:Wnk2	APN	13	49,256,278 (GRCm39)	missense	probably damaging	1.00
IGL02743:Wnk2	APN	13	49,248,920 (GRCm39)	missense	probably damaging	1.00
IGL03012:Wnk2	APN	13	49,197,865 (GRCm39)	missense	probably damaging	0.99
IGL03166:Wnk2	APN	13	49,224,520 (GRCm39)	nonsense	probably null
R0034:Wnk2	UTSW	13	49,221,556 (GRCm39)	missense	possibly damaging	0.64
R0385:Wnk2	UTSW	13	49,221,604 (GRCm39)	missense	probably damaging	1.00
R0423:Wnk2	UTSW	13	49,248,894 (GRCm39)	missense	possibly damaging	0.91
R0504:Wnk2	UTSW	13	49,238,872 (GRCm39)	missense	probably damaging	1.00
R0504:Wnk2	UTSW	13	49,238,870 (GRCm39)	missense	possibly damaging	0.92
R0653:Wnk2	UTSW	13	49,210,492 (GRCm39)	missense	possibly damaging	0.85
R1135:Wnk2	UTSW	13	49,230,034 (GRCm39)	missense	probably damaging	1.00
R1445:Wnk2	UTSW	13	49,224,586 (GRCm39)	missense	probably damaging	1.00
R1464:Wnk2	UTSW	13	49,235,451 (GRCm39)	missense	probably damaging	1.00
R1464:Wnk2	UTSW	13	49,235,451 (GRCm39)	missense	probably damaging	1.00
R1468:Wnk2	UTSW	13	49,235,571 (GRCm39)	missense	probably damaging	0.99
R1468:Wnk2	UTSW	13	49,235,571 (GRCm39)	missense	probably damaging	0.99
R1480:Wnk2	UTSW	13	49,210,708 (GRCm39)	missense	probably damaging	1.00
R1605:Wnk2	UTSW	13	49,214,370 (GRCm39)	missense	probably damaging	1.00
R1719:Wnk2	UTSW	13	49,214,202 (GRCm39)	missense	possibly damaging	0.76
R1891:Wnk2	UTSW	13	49,206,200 (GRCm39)	nonsense	probably null
R1966:Wnk2	UTSW	13	49,192,487 (GRCm39)	missense	probably damaging	0.96
R2001:Wnk2	UTSW	13	49,232,158 (GRCm39)	missense	possibly damaging	0.61
R2310:Wnk2	UTSW	13	49,204,053 (GRCm39)	missense	probably damaging	0.97
R2356:Wnk2	UTSW	13	49,192,644 (GRCm39)	nonsense	probably null
R2406:Wnk2	UTSW	13	49,214,964 (GRCm39)	missense	possibly damaging	0.86
R3962:Wnk2	UTSW	13	49,224,453 (GRCm39)	missense	probably damaging	1.00
R4160:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4161:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4226:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4227:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4234:Wnk2	UTSW	13	49,214,604 (GRCm39)	missense	probably benign	0.33
R4304:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4308:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4584:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4655:Wnk2	UTSW	13	49,210,359 (GRCm39)	missense	probably damaging	1.00
R4715:Wnk2	UTSW	13	49,300,708 (GRCm39)	start codon destroyed	unknown
R4887:Wnk2	UTSW	13	49,224,478 (GRCm39)	missense	probably damaging	1.00
R4888:Wnk2	UTSW	13	49,224,478 (GRCm39)	missense	probably damaging	1.00
R4945:Wnk2	UTSW	13	49,210,722 (GRCm39)	missense	probably damaging	1.00
R5182:Wnk2	UTSW	13	49,214,637 (GRCm39)	missense	possibly damaging	0.92
R5243:Wnk2	UTSW	13	49,226,054 (GRCm39)	missense	possibly damaging	0.51
R5370:Wnk2	UTSW	13	49,256,437 (GRCm39)	missense	probably damaging	1.00
R5771:Wnk2	UTSW	13	49,256,276 (GRCm39)	missense	probably damaging	1.00
R5877:Wnk2	UTSW	13	49,220,782 (GRCm39)	missense	probably damaging	0.98
R5900:Wnk2	UTSW	13	49,256,308 (GRCm39)	missense	probably damaging	1.00
R5905:Wnk2	UTSW	13	49,229,821 (GRCm39)	missense	probably damaging	0.99
R5912:Wnk2	UTSW	13	49,214,250 (GRCm39)	missense	probably damaging	1.00
R5915:Wnk2	UTSW	13	49,231,561 (GRCm39)	missense	probably damaging	0.99
R6028:Wnk2	UTSW	13	49,229,821 (GRCm39)	missense	probably damaging	0.99
R6074:Wnk2	UTSW	13	49,204,875 (GRCm39)	missense	probably damaging	1.00
R6171:Wnk2	UTSW	13	49,214,308 (GRCm39)	missense	probably damaging	1.00
R6368:Wnk2	UTSW	13	49,214,814 (GRCm39)	missense	probably damaging	0.99
R6467:Wnk2	UTSW	13	49,300,081 (GRCm39)	missense	probably damaging	1.00
R6501:Wnk2	UTSW	13	49,300,159 (GRCm39)	missense	probably damaging	1.00
R6849:Wnk2	UTSW	13	49,220,834 (GRCm39)	missense	probably damaging	1.00
R6898:Wnk2	UTSW	13	49,224,557 (GRCm39)	missense	probably damaging	1.00
R6949:Wnk2	UTSW	13	49,254,616 (GRCm39)	missense	probably damaging	1.00
R7011:Wnk2	UTSW	13	49,224,567 (GRCm39)	missense	probably damaging	0.99
R7097:Wnk2	UTSW	13	49,256,314 (GRCm39)	missense	possibly damaging	0.86
R7121:Wnk2	UTSW	13	49,300,653 (GRCm39)	missense	probably benign	0.26
R7123:Wnk2	UTSW	13	49,235,462 (GRCm39)	missense	possibly damaging	0.90
R7423:Wnk2	UTSW	13	49,191,608 (GRCm39)	missense	probably benign	0.07
R7502:Wnk2	UTSW	13	49,300,720 (GRCm39)	splice site	probably null
R7529:Wnk2	UTSW	13	49,254,457 (GRCm39)	missense	possibly damaging	0.50
R7751:Wnk2	UTSW	13	49,231,493 (GRCm39)	missense	unknown
R7979:Wnk2	UTSW	13	49,248,884 (GRCm39)	missense	probably damaging	1.00
R8118:Wnk2	UTSW	13	49,244,459 (GRCm39)	missense	probably damaging	0.99
R8121:Wnk2	UTSW	13	49,214,415 (GRCm39)	nonsense	probably null
R8155:Wnk2	UTSW	13	49,192,577 (GRCm39)	missense	unknown
R8329:Wnk2	UTSW	13	49,248,914 (GRCm39)	missense	probably damaging	1.00
R8334:Wnk2	UTSW	13	49,203,958 (GRCm39)	critical splice donor site	probably null
R8872:Wnk2	UTSW	13	49,210,960 (GRCm39)	missense	probably benign	0.00
R8919:Wnk2	UTSW	13	49,221,711 (GRCm39)	missense	possibly damaging	0.86
R9091:Wnk2	UTSW	13	49,224,505 (GRCm39)	missense	probably benign	0.41
R9234:Wnk2	UTSW	13	49,224,274 (GRCm39)	missense	probably damaging	0.99
R9262:Wnk2	UTSW	13	49,221,430 (GRCm39)	missense	probably benign	0.12
R9268:Wnk2	UTSW	13	49,235,507 (GRCm39)	missense	possibly damaging	0.82
R9270:Wnk2	UTSW	13	49,224,505 (GRCm39)	missense	probably benign	0.41
R9386:Wnk2	UTSW	13	49,220,822 (GRCm39)	missense	probably damaging	0.98
R9582:Wnk2	UTSW	13	49,210,975 (GRCm39)	missense	probably benign	0.01
R9617:Wnk2	UTSW	13	49,192,453 (GRCm39)	missense	unknown
R9625:Wnk2	UTSW	13	49,254,445 (GRCm39)	missense	probably benign	0.20
R9794:Wnk2	UTSW	13	49,229,674 (GRCm39)	missense	probably benign	0.02
RF023:Wnk2	UTSW	13	49,300,255 (GRCm39)	missense	probably benign	0.00
X0025:Wnk2	UTSW	13	49,214,418 (GRCm39)	missense	probably damaging	0.99
X0063:Wnk2	UTSW	13	49,192,453 (GRCm39)	missense	unknown
Z1176:Wnk2	UTSW	13	49,191,537 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- AGGTCAAACTTGAAGGTCACC -3'
(R):5'- GCACTTAAAGCTCCAAGGCTG -3'

Sequencing Primer
(F):5'- GGTTATGCGTTTCCAGCTGACAC -3'
(R):5'- CCAAGGCTGGACTGACTAGAGTG -3'

Posted On

2014-12-04