Mutagenetix > Incidental Mutation

Incidental Mutation 'R2568:Foxo6'

254617

Institutional Source

Beutler Lab

Gene Symbol

Foxo6

Ensembl Gene

ENSMUSG00000052135

Gene Name

forkhead box O6

Synonyms

MMRRC Submission

040427-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.389) question?

Stock #

R2568 (G1)

Quality Score

139

Status

Not validated

Chromosome

Chromosomal Location

120124275-120144458 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 120125961 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 278 (M278K)

Ref Sequence

ENSEMBL: ENSMUSP00000099716 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000102656]

AlphaFold

Q70KY4

Predicted Effect

probably benign
Transcript: ENSMUST00000102656
AA Change: M278K

PolyPhen 2 Score 0.108 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000099716
Gene: ENSMUSG00000052135
AA Change: M278K

Domain	Start	End	E-Value	Type
low complexity region	33	49	N/A	INTRINSIC
low complexity region	55	69	N/A	INTRINSIC
FH	86	176	1.64e-42	SMART
low complexity region	225	239	N/A	INTRINSIC
low complexity region	255	290	N/A	INTRINSIC
low complexity region	301	319	N/A	INTRINSIC
Pfam:FOXO_KIX_bdg	354	423	1.1e-13	PFAM
Pfam:FOXO-TAD	497	538	7.3e-24	PFAM

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.0%
20x: 94.1%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Homozygotes for a null allele show defective memory consolidation with impaired neuronal synchronization and altered dendritic spine morphology. Homozygotes for another null allele show attenuated gluconeogenesis, improved glucose tolerance and increased insulin sensitivity after high fat feeding. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A730071L15Rik	A	T	11: 6,150,161 (GRCm39)		probably benign	Het
Abca13	A	T	11: 9,283,310 (GRCm39)	N3244I	probably benign	Het
Adgrf5	A	G	17: 43,748,562 (GRCm39)	T219A	probably damaging	Het
Adgrg5	A	T	8: 95,660,649 (GRCm39)	N92I	probably damaging	Het
Agt	A	C	8: 125,283,694 (GRCm39)	V475G	probably damaging	Het
Akap6	A	G	12: 52,934,061 (GRCm39)	K518E	possibly damaging	Het
Apoh	T	G	11: 108,295,697 (GRCm39)	D133E	probably benign	Het
Axdnd1	T	A	1: 156,220,319 (GRCm39)	M234L	possibly damaging	Het
Cacna1d	A	G	14: 29,804,468 (GRCm39)	I1335T	probably damaging	Het
Cdh15	G	A	8: 123,588,763 (GRCm39)	R279Q	probably damaging	Het
Cfap206	T	A	4: 34,711,566 (GRCm39)	K444*	probably null	Het
Clasp2	A	G	9: 113,707,832 (GRCm39)	I614M	probably benign	Het
Col6a4	A	T	9: 105,940,275 (GRCm39)	D1218E	possibly damaging	Het
Cplane1	T	C	15: 8,230,753 (GRCm39)	V1010A	possibly damaging	Het
Csmd3	CCTTTGCGCTT	CCTT	15: 47,604,632 (GRCm39)		probably null	Het
D130043K22Rik	C	T	13: 25,067,874 (GRCm39)	T870M	probably damaging	Het
Dagla	C	A	19: 10,225,516 (GRCm39)	A883S	probably benign	Het
Dhx30	A	G	9: 109,926,263 (GRCm39)	V87A	probably damaging	Het
Dtx1	C	A	5: 120,848,249 (GRCm39)	V44L	possibly damaging	Het
Ecm2	T	A	13: 49,683,605 (GRCm39)	S528T	possibly damaging	Het
Eeig2	T	A	3: 108,886,164 (GRCm39)	N356I	probably benign	Het
Egfem1	A	T	3: 29,637,080 (GRCm39)	N172I	probably damaging	Het
Fam13a	T	G	6: 58,912,594 (GRCm39)	R686S	probably damaging	Het
Fam243	A	G	16: 92,118,207 (GRCm39)	L27P	probably damaging	Het
Fmo1	T	A	1: 162,663,828 (GRCm39)	I234L	probably benign	Het
Foxj2	C	T	6: 122,805,331 (GRCm39)	R68W	probably damaging	Het
Fsip2	A	G	2: 82,820,775 (GRCm39)	S5503G	probably benign	Het
Gdf5	C	G	2: 155,784,010 (GRCm39)	R100G	probably benign	Het
Il1b	A	T	2: 129,209,242 (GRCm39)	D129E	probably damaging	Het
Klhl29	A	G	12: 5,141,350 (GRCm39)	S545P	probably damaging	Het
Krt87	G	T	15: 101,385,708 (GRCm39)	R296S	possibly damaging	Het
Llgl1	T	G	11: 60,599,638 (GRCm39)	S509R	probably damaging	Het
Lmod1	A	T	1: 135,291,702 (GRCm39)	K186*	probably null	Het
Lrpprc	C	T	17: 85,034,077 (GRCm39)	A973T	probably damaging	Het
Marco	T	C	1: 120,422,514 (GRCm39)	H49R	possibly damaging	Het
Mylk4	T	C	13: 32,906,001 (GRCm39)	N394S	probably null	Het
Myo5a	A	G	9: 75,030,322 (GRCm39)	Y147C	probably damaging	Het
Myo5a	T	C	9: 75,059,179 (GRCm39)	V469A	probably damaging	Het
Myot	A	G	18: 44,470,283 (GRCm39)	T87A	probably benign	Het
Nav2	A	G	7: 49,247,312 (GRCm39)	H2154R	probably damaging	Het
Nek10	A	G	14: 14,999,112 (GRCm38)	E1037G	possibly damaging	Het
Or1j13	T	C	2: 36,369,986 (GRCm39)	D52G	probably damaging	Het
Or52e4	A	G	7: 104,705,878 (GRCm39)	T142A	probably benign	Het
Or5k17	A	G	16: 58,746,286 (GRCm39)	V216A	probably benign	Het
Pitrm1	G	T	13: 6,625,128 (GRCm39)	V869F	probably benign	Het
Plekhm3	CCTGCTGCTGCTGCTGCTGCTGCTGC	CCTGCTGCTGCTGCTGCTGCTGC	1: 64,976,940 (GRCm39)		probably benign	Het
Prdx1	T	G	4: 116,550,997 (GRCm39)	I156S	probably benign	Het
Rbks	T	A	5: 31,823,096 (GRCm39)	T107S	probably damaging	Het
Ryr3	G	A	2: 112,506,219 (GRCm39)	R3468W	probably damaging	Het
Scn1a	C	T	2: 66,103,813 (GRCm39)	D1805N	probably damaging	Het
Sirpa	T	C	2: 129,457,568 (GRCm39)	V214A	probably benign	Het
Slc35c1	T	C	2: 92,289,225 (GRCm39)	N94D	probably benign	Het
Sorbs2	A	T	8: 46,248,407 (GRCm39)	K553*	probably null	Het
Tectb	C	G	19: 55,169,431 (GRCm39)		probably benign	Het
Thg1l	A	G	11: 45,842,392 (GRCm39)	V142A	probably benign	Het
Tiparp	T	A	3: 65,460,551 (GRCm39)	Y513*	probably null	Het
Tmc6	A	G	11: 117,663,646 (GRCm39)	V522A	probably benign	Het
Trim39	T	A	17: 36,580,056 (GRCm39)		probably benign	Het
Trrap	G	A	5: 144,780,179 (GRCm39)		probably null	Het
Tulp3	C	T	6: 128,304,601 (GRCm39)	V218I	probably benign	Het
Vmn1r38	T	A	6: 66,753,955 (GRCm39)	I54F	probably benign	Het
Vmn2r23	T	A	6: 123,719,147 (GRCm39)	Y833*	probably null	Het
Zfp810	A	T	9: 22,190,534 (GRCm39)	S125T	probably benign	Het

Other mutations in Foxo6

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01124:Foxo6	APN	4	120,126,349 (GRCm39)	missense	probably benign	0.09
IGL02441:Foxo6	APN	4	120,125,232 (GRCm39)	missense	possibly damaging	0.52
R0284:Foxo6	UTSW	4	120,126,199 (GRCm39)	missense	probably benign	0.01
R2044:Foxo6	UTSW	4	120,144,166 (GRCm39)	missense	probably benign	0.40
R2152:Foxo6	UTSW	4	120,125,811 (GRCm39)	missense	probably benign	0.03
R3008:Foxo6	UTSW	4	120,125,961 (GRCm39)	missense	probably benign	0.11
R7352:Foxo6	UTSW	4	120,125,448 (GRCm39)	missense	probably benign	0.02
R8724:Foxo6	UTSW	4	120,144,109 (GRCm39)	missense	probably damaging	1.00
R8951:Foxo6	UTSW	4	120,125,133 (GRCm39)	missense	unknown
Z1187:Foxo6	UTSW	4	120,144,332 (GRCm39)	missense	possibly damaging	0.93
Z1188:Foxo6	UTSW	4	120,144,332 (GRCm39)	missense	possibly damaging	0.93

Predicted Primers

PCR Primer
(F):5'- TCTCCTGGATGGTCTGCATG -3'
(R):5'- CCAAGTTCCTGCGCATCAAG -3'

Sequencing Primer
(F):5'- TTGCAGCTGCCGAAGTAG -3'
(R):5'- AGAAGCAGCTGCACCTG -3'

Posted On

2014-12-04