Incidental Mutation 'R2932:Ccdc15'
ID 254976
Institutional Source Beutler Lab
Gene Symbol Ccdc15
Ensembl Gene ENSMUSG00000034303
Gene Name coiled-coil domain containing 15
Synonyms A630039F14Rik
Accession Numbers
Essential gene? Probably non essential (E-score: 0.099) question?
Stock # R2932 (G1)
Quality Score 225
Status Not validated
Chromosome 9
Chromosomal Location 37187131-37259728 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to A at 37226954 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Threonine to Isoleucine at position 327 (T327I)
Ref Sequence ENSEMBL: ENSMUSP00000150207 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000037275] [ENSMUST00000213633] [ENSMUST00000215116]
AlphaFold Q8C9M2
Predicted Effect probably benign
Transcript: ENSMUST00000037275
AA Change: T340I

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)
SMART Domains Protein: ENSMUSP00000036784
Gene: ENSMUSG00000034303
AA Change: T340I

DomainStartEndE-ValueType
low complexity region 80 92 N/A INTRINSIC
low complexity region 95 109 N/A INTRINSIC
coiled coil region 173 202 N/A INTRINSIC
coiled coil region 652 686 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000213633
AA Change: T327I

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)
Predicted Effect probably benign
Transcript: ENSMUST00000215116
Predicted Effect noncoding transcript
Transcript: ENSMUST00000216060
Predicted Effect noncoding transcript
Transcript: ENSMUST00000217440
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.2%
  • 20x: 94.7%
Validation Efficiency
Allele List at MGI

All alleles(2) : Gene trapped(2)
Other mutations in this stock
Total: 20 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Alms1 A G 6: 85,597,544 (GRCm39) D1259G possibly damaging Het
Arpp21 T C 9: 112,008,173 (GRCm39) D106G probably damaging Het
Atp6v1a A G 16: 43,909,406 (GRCm39) S542P probably benign Het
Ccar1 T C 10: 62,612,538 (GRCm39) N209S probably benign Het
Dhx15 G A 5: 52,324,074 (GRCm39) P406L probably benign Het
Fat3 G A 9: 16,287,240 (GRCm39) S761L probably damaging Het
Loricrin AGCCGCCGCCGCCGCCGCCGCCGCCGCC AGCCGCCGCCGCCGCCGCCGCCGCC 3: 91,989,185 (GRCm39) probably benign Het
Lrrc1 T A 9: 77,364,721 (GRCm39) H153L probably benign Het
Lrrc46 T C 11: 96,931,935 (GRCm39) probably benign Het
Mrgprb2 A G 7: 48,202,194 (GRCm39) L177S probably benign Het
Mtmr2 A T 9: 13,660,413 (GRCm39) probably benign Het
Mtnr1b A T 9: 15,785,620 (GRCm39) V46E probably damaging Het
Myo5a A G 9: 75,103,418 (GRCm39) E207G possibly damaging Het
Nalcn A G 14: 123,830,430 (GRCm39) S137P probably benign Het
Nipal1 A G 5: 72,824,978 (GRCm39) I224V possibly damaging Het
Oas1g T G 5: 121,017,206 (GRCm39) K283Q probably benign Het
Pex12 T A 11: 83,187,049 (GRCm39) M300L probably benign Het
Phldb2 A G 16: 45,569,148 (GRCm39) V1237A possibly damaging Het
Rc3h2 C A 2: 37,268,371 (GRCm39) V920F probably benign Het
Ulk1 C T 5: 110,937,223 (GRCm39) R691Q probably benign Het
Other mutations in Ccdc15
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00503:Ccdc15 APN 9 37,231,769 (GRCm39) missense probably damaging 1.00
IGL00823:Ccdc15 APN 9 37,231,709 (GRCm39) missense probably benign 0.01
IGL00979:Ccdc15 APN 9 37,227,786 (GRCm39) missense probably benign 0.44
IGL01380:Ccdc15 APN 9 37,187,853 (GRCm39) utr 3 prime probably benign
IGL01832:Ccdc15 APN 9 37,222,640 (GRCm39) missense probably damaging 0.97
IGL01845:Ccdc15 APN 9 37,226,532 (GRCm39) nonsense probably null
IGL02375:Ccdc15 APN 9 37,215,628 (GRCm39) missense probably damaging 0.99
F6893:Ccdc15 UTSW 9 37,226,936 (GRCm39) missense probably damaging 0.97
FR4304:Ccdc15 UTSW 9 37,226,453 (GRCm39) frame shift probably null
FR4449:Ccdc15 UTSW 9 37,226,454 (GRCm39) frame shift probably null
R1743:Ccdc15 UTSW 9 37,188,773 (GRCm39) nonsense probably null
R1848:Ccdc15 UTSW 9 37,253,866 (GRCm39) missense probably benign 0.00
R1968:Ccdc15 UTSW 9 37,259,091 (GRCm39) missense probably benign 0.05
R2006:Ccdc15 UTSW 9 37,226,768 (GRCm39) missense possibly damaging 0.91
R2372:Ccdc15 UTSW 9 37,226,801 (GRCm39) missense possibly damaging 0.64
R3962:Ccdc15 UTSW 9 37,231,782 (GRCm39) missense probably damaging 1.00
R5585:Ccdc15 UTSW 9 37,188,699 (GRCm39) missense probably benign 0.00
R5919:Ccdc15 UTSW 9 37,231,692 (GRCm39) critical splice donor site probably null
R6000:Ccdc15 UTSW 9 37,227,060 (GRCm39) missense probably benign 0.00
R6198:Ccdc15 UTSW 9 37,225,581 (GRCm39) critical splice donor site probably null
R6476:Ccdc15 UTSW 9 37,253,715 (GRCm39) missense probably benign 0.04
R7098:Ccdc15 UTSW 9 37,255,256 (GRCm39) missense probably damaging 1.00
R7485:Ccdc15 UTSW 9 37,226,574 (GRCm39) missense probably benign
R7548:Ccdc15 UTSW 9 37,188,723 (GRCm39) missense probably benign 0.45
R7627:Ccdc15 UTSW 9 37,253,698 (GRCm39) missense unknown
R7807:Ccdc15 UTSW 9 37,226,678 (GRCm39) missense probably benign 0.07
R8157:Ccdc15 UTSW 9 37,226,753 (GRCm39) missense probably benign
R8230:Ccdc15 UTSW 9 37,226,555 (GRCm39) missense probably benign
R9492:Ccdc15 UTSW 9 37,215,665 (GRCm39) missense probably damaging 1.00
R9645:Ccdc15 UTSW 9 37,227,083 (GRCm39) missense probably benign 0.00
Predicted Primers PCR Primer
(F):5'- GAGAACACAGTGACCTTTGGG -3'
(R):5'- GATGAACGAAAGCAATTACATCCTC -3'

Sequencing Primer
(F):5'- ACCTTTGGGTAAAGAATCCTGG -3'
(R):5'- GCAATTACATCCTCATAGCCTTCAGG -3'
Posted On 2014-12-29