Incidental Mutation 'R2941:Fgr'
ID 255172
Institutional Source Beutler Lab
Gene Symbol Fgr
Ensembl Gene ENSMUSG00000028874
Gene Name FGR proto-oncogene, Src family tyrosine kinase
Synonyms Ali18, Mhdaali18
Accession Numbers
Essential gene? Probably non essential (E-score: 0.195) question?
Stock # R2941 (G1)
Quality Score 225
Status Not validated
Chromosome 4
Chromosomal Location 132701406-132729204 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to C at 132725734 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Tyrosine to Serine at position 381 (Y381S)
Ref Sequence ENSEMBL: ENSMUSP00000128411 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000030693] [ENSMUST00000171223]
AlphaFold P14234
Predicted Effect probably benign
Transcript: ENSMUST00000030693
AA Change: Y381S

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000030693
Gene: ENSMUSG00000028874
AA Change: Y381S

DomainStartEndE-ValueType
SH3 68 125 5.39e-22 SMART
SH2 130 220 5.25e-36 SMART
TyrKc 251 500 5.5e-126 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000138179
Predicted Effect probably benign
Transcript: ENSMUST00000171223
AA Change: Y381S

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000128411
Gene: ENSMUSG00000028874
AA Change: Y381S

DomainStartEndE-ValueType
SH3 68 125 5.39e-22 SMART
SH2 130 220 5.25e-36 SMART
TyrKc 251 500 5.5e-126 SMART
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.5%
  • 10x: 97.1%
  • 20x: 94.6%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway. Infection with Epstein-Barr virus results in the overexpression of this gene. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit a partial reduction in hemorrhage following induction of a local Shwartzman reaction, and show enhanced NK-cell receptor-induced IFN-gamma production in natural killer (NK) cells. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 15 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Afg1l G T 10: 42,354,291 (GRCm39) probably null Het
Ankrd60 A G 2: 173,410,674 (GRCm39) L282P probably damaging Het
Avl9 T C 6: 56,730,870 (GRCm39) V598A probably benign Het
Dgkb T A 12: 38,654,122 (GRCm39) M747K possibly damaging Het
Elmo1 C G 13: 20,784,382 (GRCm39) Y646* probably null Het
Ggnbp2 T C 11: 84,732,407 (GRCm39) I271V probably damaging Het
Itprid2 T A 2: 79,466,000 (GRCm39) D39E probably benign Het
Ltbp1 A G 17: 75,486,088 (GRCm39) T38A probably damaging Het
Or8b52 A G 9: 38,576,322 (GRCm39) S273P probably damaging Het
Setbp1 C A 18: 78,901,412 (GRCm39) V752L probably damaging Het
Slc8a3 C A 12: 81,361,953 (GRCm39) G289W probably damaging Het
Slu7 G T 11: 43,335,584 (GRCm39) R440L probably benign Het
Tacr1 T A 6: 82,380,715 (GRCm39) I42N probably damaging Het
Tspan32 G A 7: 142,568,729 (GRCm39) V103M probably damaging Het
Tyk2 A G 9: 21,022,415 (GRCm39) S805P probably benign Het
Other mutations in Fgr
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02201:Fgr APN 4 132,722,235 (GRCm39) missense probably damaging 0.99
IGL03089:Fgr APN 4 132,713,577 (GRCm39) missense probably damaging 0.96
R1760:Fgr UTSW 4 132,725,653 (GRCm39) missense possibly damaging 0.72
R1957:Fgr UTSW 4 132,725,673 (GRCm39) missense probably benign
R2011:Fgr UTSW 4 132,724,832 (GRCm39) missense probably damaging 1.00
R2109:Fgr UTSW 4 132,725,786 (GRCm39) missense probably benign 0.32
R2351:Fgr UTSW 4 132,724,548 (GRCm39) missense probably damaging 0.99
R3034:Fgr UTSW 4 132,725,807 (GRCm39) critical splice donor site probably null
R4590:Fgr UTSW 4 132,722,364 (GRCm39) missense probably damaging 1.00
R4770:Fgr UTSW 4 132,714,602 (GRCm39) missense probably damaging 0.99
R4847:Fgr UTSW 4 132,721,959 (GRCm39) missense probably damaging 1.00
R5294:Fgr UTSW 4 132,724,811 (GRCm39) missense probably benign 0.01
R5384:Fgr UTSW 4 132,713,664 (GRCm39) critical splice donor site probably null
R5388:Fgr UTSW 4 132,722,342 (GRCm39) missense probably damaging 1.00
R5650:Fgr UTSW 4 132,727,533 (GRCm39) missense probably benign 0.13
R6947:Fgr UTSW 4 132,722,380 (GRCm39) critical splice donor site probably null
R7651:Fgr UTSW 4 132,722,324 (GRCm39) missense probably damaging 1.00
R7686:Fgr UTSW 4 132,725,324 (GRCm39) missense probably benign
R7921:Fgr UTSW 4 132,713,832 (GRCm39) splice site probably null
R8011:Fgr UTSW 4 132,725,790 (GRCm39) missense probably damaging 1.00
R8238:Fgr UTSW 4 132,724,832 (GRCm39) missense probably damaging 1.00
R8742:Fgr UTSW 4 132,724,828 (GRCm39) missense probably damaging 1.00
R8876:Fgr UTSW 4 132,726,071 (GRCm39) intron probably benign
R8884:Fgr UTSW 4 132,713,609 (GRCm39) missense probably benign 0.01
Z1176:Fgr UTSW 4 132,727,481 (GRCm39) missense probably benign 0.23
Predicted Primers PCR Primer
(F):5'- CCATCGGGACAGTGTAGTTTTC -3'
(R):5'- GGGGAGACTTGAATTCAGAATTGC -3'

Sequencing Primer
(F):5'- AGTGTAGTTTTCAGGAGACTCCCAC -3'
(R):5'- TGTGACTCACATCAGGATCG -3'
Posted On 2014-12-29