Mutagenetix > Incidental Mutation

Incidental Mutation 'R2926:Vmn2r73'

255759

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r73

Ensembl Gene

ENSMUSG00000070458

Gene Name

vomeronasal 2, receptor 73

Synonyms

EG620928

MMRRC Submission

040511-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.141) question?

Stock #

R2926 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

85506755-85525146 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 85520871 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Glutamic Acid at position 366 (K366E)

Ref Sequence

ENSEMBL: ENSMUSP00000076687 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077478]

AlphaFold

D3Z7M3

Predicted Effect

probably benign
Transcript: ENSMUST00000077478
AA Change: K366E

PolyPhen 2 Score 0.168 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000076687
Gene: ENSMUSG00000070458
AA Change: K366E

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	465	1.4e-30	PFAM
Pfam:NCD3G	508	560	5.9e-21	PFAM
Pfam:7tm_3	590	827	4.4e-51	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.3%

Validation Efficiency

98% (50/51)

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb9	G	T	5: 124,216,902 (GRCm39)	S438R	possibly damaging	Het
Add3	A	G	19: 53,215,253 (GRCm39)		probably null	Het
Adgrb2	T	C	4: 129,902,137 (GRCm39)	L506P	probably damaging	Het
Atp6v0a1	A	T	11: 100,934,774 (GRCm39)	I621L	probably damaging	Het
Calb1	T	G	4: 15,904,302 (GRCm39)	L218R	probably damaging	Het
Ccdc162	G	A	10: 41,437,203 (GRCm39)		probably benign	Het
Ccser2	A	T	14: 36,601,518 (GRCm39)	S842T	possibly damaging	Het
Cd300a	A	G	11: 114,784,139 (GRCm39)	E49G	possibly damaging	Het
Colec11	T	A	12: 28,667,428 (GRCm39)	Q37L	probably damaging	Het
D630045J12Rik	T	A	6: 38,145,106 (GRCm39)	I1307F	probably damaging	Het
Dapk1	T	C	13: 60,867,564 (GRCm39)	V257A	possibly damaging	Het
Dnah3	T	A	7: 119,550,338 (GRCm39)	N3327I	probably damaging	Het
Gja8	C	T	3: 96,826,469 (GRCm39)	V398I	probably benign	Het
Hfm1	A	T	5: 107,022,148 (GRCm39)	L179*	probably null	Het
Hsd3b9	A	G	3: 98,357,872 (GRCm39)		probably benign	Het
Ift88	T	C	14: 57,726,375 (GRCm39)	Y678H	probably damaging	Het
Itga10	A	G	3: 96,560,165 (GRCm39)	N560D	probably damaging	Het
Itpk1	G	T	12: 102,545,389 (GRCm39)	P238Q	probably damaging	Het
Kl	T	C	5: 150,876,806 (GRCm39)	W209R	probably damaging	Het
Lama4	A	G	10: 38,954,828 (GRCm39)	N1127S	probably benign	Het
Lrp1	C	T	10: 127,423,982 (GRCm39)	C830Y	probably damaging	Het
Mcmbp	G	A	7: 128,299,738 (GRCm39)		probably benign	Het
Mrps33	A	G	6: 39,782,438 (GRCm39)	S28P	probably damaging	Het
Myo9b	G	A	8: 71,786,981 (GRCm39)	R721Q	probably benign	Het
Myt1	C	T	2: 181,467,803 (GRCm39)	T1079M	possibly damaging	Het
N4bp1	A	T	8: 87,588,424 (GRCm39)	Y171*	probably null	Het
Ncln	G	T	10: 81,324,272 (GRCm39)	T442K	probably benign	Het
Nphp4	T	C	4: 152,602,596 (GRCm39)	V390A	probably damaging	Het
Ntrk2	C	A	13: 59,208,098 (GRCm39)	T648K	probably damaging	Het
Nwd1	A	G	8: 73,393,640 (GRCm39)	H301R	probably damaging	Het
Or4k5	A	T	14: 50,385,893 (GRCm39)	V146E	probably benign	Het
Pcdh12	T	C	18: 38,415,443 (GRCm39)	N561D	probably damaging	Het
Pcnx1	T	A	12: 82,041,769 (GRCm39)	S2134T	probably damaging	Het
Ppp1cc	G	A	5: 122,312,151 (GRCm39)	A306T	probably benign	Het
Pramel3e	G	T	X: 134,400,297 (GRCm39)	A96S	possibly damaging	Het
Prrc2c	C	T	1: 162,533,696 (GRCm39)		probably benign	Het
Rabggta	C	T	14: 55,956,747 (GRCm39)	R319H	probably benign	Het
Scn10a	A	C	9: 119,467,767 (GRCm39)	F791C	possibly damaging	Het
Stab1	T	A	14: 30,883,756 (GRCm39)	D267V	probably damaging	Het
Sva	A	T	6: 42,019,596 (GRCm39)	Y152F	possibly damaging	Het
Tgfbrap1	T	G	1: 43,114,789 (GRCm39)	M104L	probably damaging	Het
Tmed4	T	C	11: 6,221,728 (GRCm39)	T203A	probably benign	Het
Toe1	C	T	4: 116,662,177 (GRCm39)	A331T	possibly damaging	Het
Trappc9	G	A	15: 72,897,816 (GRCm39)	R377W	probably damaging	Het
Trpm7	T	C	2: 126,700,329 (GRCm39)		probably benign	Het
Ttll7	C	T	3: 146,636,170 (GRCm39)	R438*	probably null	Het
Usp11	G	T	X: 20,584,031 (GRCm39)	G601W	probably damaging	Het
Vmn2r112	A	G	17: 22,833,984 (GRCm39)	T551A	possibly damaging	Het
Vps33a	A	G	5: 123,707,634 (GRCm39)	I111T	possibly damaging	Het

Other mutations in Vmn2r73

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00158:Vmn2r73	APN	7	85,506,795 (GRCm39)	missense
IGL01151:Vmn2r73	APN	7	85,507,086 (GRCm39)	missense	probably damaging	0.99
IGL01408:Vmn2r73	APN	7	85,521,455 (GRCm39)	missense	probably benign	0.45
IGL01505:Vmn2r73	APN	7	85,507,267 (GRCm39)	nonsense	probably null
IGL01731:Vmn2r73	APN	7	85,506,757 (GRCm39)	makesense	probably null
IGL01818:Vmn2r73	APN	7	85,519,109 (GRCm39)	splice site	probably benign
IGL02236:Vmn2r73	APN	7	85,521,902 (GRCm39)	missense	probably benign	0.00
IGL02265:Vmn2r73	APN	7	85,520,847 (GRCm39)	missense	probably benign	0.02
IGL02266:Vmn2r73	APN	7	85,525,007 (GRCm39)	missense	possibly damaging	0.52
IGL02276:Vmn2r73	APN	7	85,518,980 (GRCm39)	critical splice donor site	probably null
IGL02284:Vmn2r73	APN	7	85,507,123 (GRCm39)	missense	possibly damaging	0.92
IGL02380:Vmn2r73	APN	7	85,507,383 (GRCm39)	missense	probably benign	0.00
IGL02466:Vmn2r73	APN	7	85,522,084 (GRCm39)	missense	probably damaging	1.00
IGL03172:Vmn2r73	APN	7	85,507,495 (GRCm39)	missense	probably benign	0.01
IGL03179:Vmn2r73	APN	7	85,519,468 (GRCm39)	missense	probably benign	0.04
G1Funyon:Vmn2r73	UTSW	7	85,507,510 (GRCm39)	missense	probably benign	0.00
R0077:Vmn2r73	UTSW	7	85,525,075 (GRCm39)	missense	probably benign	0.00
R0311:Vmn2r73	UTSW	7	85,520,997 (GRCm39)	missense	probably benign
R0413:Vmn2r73	UTSW	7	85,521,087 (GRCm39)	missense	possibly damaging	0.68
R0494:Vmn2r73	UTSW	7	85,522,140 (GRCm39)	missense	probably benign
R1523:Vmn2r73	UTSW	7	85,519,486 (GRCm39)	missense	probably benign	0.02
R1618:Vmn2r73	UTSW	7	85,525,120 (GRCm39)	nonsense	probably null
R1667:Vmn2r73	UTSW	7	85,506,889 (GRCm39)	missense	probably benign	0.00
R1728:Vmn2r73	UTSW	7	85,507,086 (GRCm39)	missense	probably damaging	1.00
R1729:Vmn2r73	UTSW	7	85,507,086 (GRCm39)	missense	probably damaging	1.00
R1747:Vmn2r73	UTSW	7	85,507,375 (GRCm39)	missense	probably damaging	1.00
R1784:Vmn2r73	UTSW	7	85,507,086 (GRCm39)	missense	probably damaging	1.00
R2152:Vmn2r73	UTSW	7	85,506,936 (GRCm39)	missense	probably benign	0.03
R2395:Vmn2r73	UTSW	7	85,506,975 (GRCm39)	missense	probably damaging	1.00
R2415:Vmn2r73	UTSW	7	85,521,431 (GRCm39)	missense	probably damaging	1.00
R3719:Vmn2r73	UTSW	7	85,519,582 (GRCm39)	missense	probably damaging	1.00
R3766:Vmn2r73	UTSW	7	85,521,198 (GRCm39)	missense	probably benign
R3838:Vmn2r73	UTSW	7	85,507,258 (GRCm39)	missense	probably benign	0.00
R3890:Vmn2r73	UTSW	7	85,507,144 (GRCm39)	missense	probably benign
R4030:Vmn2r73	UTSW	7	85,521,044 (GRCm39)	missense	possibly damaging	0.92
R4247:Vmn2r73	UTSW	7	85,521,883 (GRCm39)	missense	probably damaging	0.99
R4409:Vmn2r73	UTSW	7	85,520,768 (GRCm39)	missense	probably damaging	0.97
R4427:Vmn2r73	UTSW	7	85,506,981 (GRCm39)	missense	probably damaging	1.00
R4430:Vmn2r73	UTSW	7	85,519,449 (GRCm39)	missense	probably benign
R4552:Vmn2r73	UTSW	7	85,525,055 (GRCm39)	missense	probably benign	0.12
R4779:Vmn2r73	UTSW	7	85,520,923 (GRCm39)	nonsense	probably null
R4906:Vmn2r73	UTSW	7	85,506,829 (GRCm39)	missense	probably damaging	0.99
R4942:Vmn2r73	UTSW	7	85,519,582 (GRCm39)	missense	probably damaging	1.00
R5280:Vmn2r73	UTSW	7	85,507,155 (GRCm39)	missense	probably damaging	0.98
R5344:Vmn2r73	UTSW	7	85,525,046 (GRCm39)	missense	probably benign	0.00
R5478:Vmn2r73	UTSW	7	85,518,996 (GRCm39)	missense	probably damaging	1.00
R5527:Vmn2r73	UTSW	7	85,507,575 (GRCm39)	missense	possibly damaging	0.65
R5691:Vmn2r73	UTSW	7	85,507,299 (GRCm39)	missense	probably damaging	1.00
R5826:Vmn2r73	UTSW	7	85,524,956 (GRCm39)	missense	possibly damaging	0.77
R6018:Vmn2r73	UTSW	7	85,521,875 (GRCm39)	missense	possibly damaging	0.48
R6082:Vmn2r73	UTSW	7	85,507,429 (GRCm39)	missense	probably benign	0.00
R6111:Vmn2r73	UTSW	7	85,520,997 (GRCm39)	missense	probably benign
R6233:Vmn2r73	UTSW	7	85,519,099 (GRCm39)	missense	probably benign	0.00
R6278:Vmn2r73	UTSW	7	85,522,140 (GRCm39)	missense	probably benign
R6283:Vmn2r73	UTSW	7	85,521,049 (GRCm39)	missense	probably benign	0.00
R6307:Vmn2r73	UTSW	7	85,506,828 (GRCm39)	missense	probably damaging	1.00
R6413:Vmn2r73	UTSW	7	85,519,544 (GRCm39)	nonsense	probably null
R6782:Vmn2r73	UTSW	7	85,519,563 (GRCm39)	missense	probably benign	0.00
R6884:Vmn2r73	UTSW	7	85,507,213 (GRCm39)	missense	probably benign	0.05
R6921:Vmn2r73	UTSW	7	85,507,446 (GRCm39)	missense	probably benign	0.27
R7169:Vmn2r73	UTSW	7	85,507,663 (GRCm39)	nonsense	probably null
R7206:Vmn2r73	UTSW	7	85,522,075 (GRCm39)	missense	probably benign	0.03
R7311:Vmn2r73	UTSW	7	85,521,192 (GRCm39)	missense	possibly damaging	0.80
R7650:Vmn2r73	UTSW	7	85,521,147 (GRCm39)	missense	probably benign
R7811:Vmn2r73	UTSW	7	85,524,956 (GRCm39)	missense	possibly damaging	0.77
R8263:Vmn2r73	UTSW	7	85,507,619 (GRCm39)	missense	probably benign
R8301:Vmn2r73	UTSW	7	85,507,510 (GRCm39)	missense	probably benign	0.00
R8341:Vmn2r73	UTSW	7	85,507,128 (GRCm39)	missense	probably benign	0.01
R8500:Vmn2r73	UTSW	7	85,519,638 (GRCm39)	missense	probably benign	0.01
R8673:Vmn2r73	UTSW	7	85,521,902 (GRCm39)	missense	probably benign	0.01
R8719:Vmn2r73	UTSW	7	85,521,095 (GRCm39)	missense	probably damaging	0.96
R8783:Vmn2r73	UTSW	7	85,507,668 (GRCm39)	missense	probably damaging	0.96
R8828:Vmn2r73	UTSW	7	85,521,179 (GRCm39)	missense	probably damaging	0.98
R8860:Vmn2r73	UTSW	7	85,522,149 (GRCm39)	splice site	probably benign
R9002:Vmn2r73	UTSW	7	85,507,284 (GRCm39)	missense	probably benign	0.32
R9008:Vmn2r73	UTSW	7	85,521,904 (GRCm39)	missense	probably damaging	0.96
R9159:Vmn2r73	UTSW	7	85,521,931 (GRCm39)	missense	possibly damaging	0.90
R9180:Vmn2r73	UTSW	7	85,507,123 (GRCm39)	missense	probably benign	0.07
R9198:Vmn2r73	UTSW	7	85,518,987 (GRCm39)	missense	probably benign	0.01
R9329:Vmn2r73	UTSW	7	85,519,073 (GRCm39)	missense	probably benign
R9448:Vmn2r73	UTSW	7	85,522,027 (GRCm39)	missense	probably benign	0.00
R9463:Vmn2r73	UTSW	7	85,506,795 (GRCm39)	missense
R9589:Vmn2r73	UTSW	7	85,519,659 (GRCm39)	missense	probably benign	0.03
X0023:Vmn2r73	UTSW	7	85,507,664 (GRCm39)	missense	probably benign	0.31
Z1176:Vmn2r73	UTSW	7	85,521,176 (GRCm39)	missense	probably damaging	0.99
Z1177:Vmn2r73	UTSW	7	85,521,480 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GACTCCTTACCTTCCAAGAGTC -3'
(R):5'- CACACCTCTAGGAGATTTCATGC -3'

Sequencing Primer
(F):5'- CCAAGAGTCAAATTCCAGTTCCTTG -3'
(R):5'- AGGAGATTTCATGCTATACTCCGC -3'

Posted On

2014-12-29