Mutagenetix > Incidental Mutation

Incidental Mutation 'R2964:Oprm1'

255979

Institutional Source

Beutler Lab

Gene Symbol

Oprm1

Ensembl Gene

ENSMUSG00000000766

Gene Name

opioid receptor, mu 1

Synonyms

MOP-R, MOR-1, MOP receptor, mor, Oprm, muOR

MMRRC Submission

040520-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.103) question?

Stock #

R2964 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

6708593-6988209 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 6738914 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 14 (S14P)

Ref Sequence

ENSEMBL: ENSMUSP00000120187 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000000783] [ENSMUST00000052751] [ENSMUST00000056385] [ENSMUST00000063036] [ENSMUST00000078634] [ENSMUST00000092729] [ENSMUST00000092731] [ENSMUST00000105604] [ENSMUST00000092734] [ENSMUST00000105601] [ENSMUST00000105602] [ENSMUST00000105603] [ENSMUST00000105605] [ENSMUST00000105607] [ENSMUST00000105611] [ENSMUST00000123861] [ENSMUST00000129954] [ENSMUST00000105597] [ENSMUST00000105615] [ENSMUST00000129221] [ENSMUST00000135502] [ENSMUST00000143875] [ENSMUST00000144264] [ENSMUST00000152674] [ENSMUST00000154906] [ENSMUST00000154941] [ENSMUST00000147171] [ENSMUST00000150374]

AlphaFold

P42866

Predicted Effect

probably benign
Transcript: ENSMUST00000000783
AA Change: S14P

PolyPhen 2 Score 0.103 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000000783
Gene: ENSMUSG00000000766
AA Change: S14P

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	339	1.6e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1.3e-10	PFAM
Pfam:7tm_1	85	336	4e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000052751
AA Change: S14P

PolyPhen 2 Score 0.103 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000060329
Gene: ENSMUSG00000000766
AA Change: S14P

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1.1e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1e-10	PFAM
Pfam:7tm_1	85	336	3.2e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000056385
AA Change: S14P

PolyPhen 2 Score 0.103 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000060590
Gene: ENSMUSG00000000766
AA Change: S14P

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1.7e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1e-10	PFAM
Pfam:7tm_1	85	336	3.3e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000063036

SMART Domains

Protein: ENSMUSP00000053498
Gene: ENSMUSG00000000766

Domain	Start	End	E-Value	Type
Pfam:7tm_1	24	268	8.7e-64	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000078634
AA Change: S14P

PolyPhen 2 Score 0.507 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000077704
Gene: ENSMUSG00000000766
AA Change: S14P

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	339	2.1e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	2.4e-10	PFAM
Pfam:7tm_1	85	336	9e-60	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000092729
AA Change: S14P

PolyPhen 2 Score 0.103 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000090405
Gene: ENSMUSG00000000766
AA Change: S14P

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1.7e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	9.6e-11	PFAM
Pfam:7tm_1	85	336	3.1e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000092731
AA Change: S14P

PolyPhen 2 Score 0.103 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000090407
Gene: ENSMUSG00000000766
AA Change: S14P

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	9.9e-11	PFAM
Pfam:7tm_1	85	336	3.2e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000105604
AA Change: S14P

PolyPhen 2 Score 0.059 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000101229
Gene: ENSMUSG00000000766
AA Change: S14P

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	332	5.1e-8	PFAM
Pfam:7TM_GPCR_Srsx	79	351	9.9e-11	PFAM
Pfam:7tm_1	85	336	3.8e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000092734
AA Change: S14P

PolyPhen 2 Score 0.103 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000090410
Gene: ENSMUSG00000000766
AA Change: S14P

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1.7e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1e-10	PFAM
Pfam:7tm_1	85	336	3.3e-67	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000105601
AA Change: S14P

PolyPhen 2 Score 0.982 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000101226
Gene: ENSMUSG00000000766
AA Change: S14P

Domain	Start	End	E-Value	Type
SCOP:d1l9ha_	46	100	3e-9	SMART
PDB:4DKL\|A	52	100	3e-21	PDB
low complexity region	119	131	N/A	INTRINSIC
low complexity region	173	184	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000105602
AA Change: S14P

PolyPhen 2 Score 0.103 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000101227
Gene: ENSMUSG00000000766
AA Change: S14P

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1.8e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	9.8e-11	PFAM
Pfam:7tm_1	85	336	3.1e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000105603
AA Change: S14P

PolyPhen 2 Score 0.103 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000101228
Gene: ENSMUSG00000000766
AA Change: S14P

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	6.7e-8	PFAM
Pfam:7TM_GPCR_Srsx	79	351	9.6e-11	PFAM
Pfam:7tm_1	85	336	3.6e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000105605
AA Change: S14P

PolyPhen 2 Score 0.103 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000101230
Gene: ENSMUSG00000000766
AA Change: S14P

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	9.8e-11	PFAM
Pfam:7tm_1	85	336	3.2e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000105607
AA Change: S14P

PolyPhen 2 Score 0.103 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000101232
Gene: ENSMUSG00000000766
AA Change: S14P

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1.7e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1e-10	PFAM
Pfam:7tm_1	85	336	3.3e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000105611
AA Change: S14P

PolyPhen 2 Score 0.103 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000101236
Gene: ENSMUSG00000000766
AA Change: S14P

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	338	1.7e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1.4e-10	PFAM
Pfam:7tm_1	85	336	4.4e-67	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000123861
AA Change: S14P

PolyPhen 2 Score 0.982 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000120187
Gene: ENSMUSG00000000766
AA Change: S14P

Domain	Start	End	E-Value	Type
SCOP:d1l9ha_	46	100	3e-9	SMART
PDB:4DKL\|A	52	100	3e-21	PDB
low complexity region	119	131	N/A	INTRINSIC
low complexity region	173	184	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000129954
AA Change: S14P

PolyPhen 2 Score 0.653 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000122385
Gene: ENSMUSG00000000766
AA Change: S14P

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	338	6.9e-8	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1.5e-10	PFAM
Pfam:7tm_1	85	336	5.4e-67	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000133486

Predicted Effect

probably benign
Transcript: ENSMUST00000105597

Predicted Effect

probably benign
Transcript: ENSMUST00000105615

SMART Domains

Protein: ENSMUSP00000101240
Gene: ENSMUSG00000000766

Domain	Start	End	E-Value	Type
Pfam:7tm_1	24	268	1.3e-63	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000129221

SMART Domains

Protein: ENSMUSP00000123117
Gene: ENSMUSG00000000766

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	12	261	1.1e-7	PFAM
Pfam:7TM_GPCR_Srsx	15	287	7.3e-11	PFAM
Pfam:7tm_1	21	272	2.4e-67	PFAM
low complexity region	340	351	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000135502
AA Change: S14P

PolyPhen 2 Score 0.103 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000135143
Gene: ENSMUSG00000000766
AA Change: S14P

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	339	2.2e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1.9e-10	PFAM
Pfam:7tm_1	85	336	7.5e-67	PFAM

Predicted Effect

unknown
Transcript: ENSMUST00000143875
AA Change: L84P

Predicted Effect

probably benign
Transcript: ENSMUST00000144264
AA Change: S14P

PolyPhen 2 Score 0.103 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000115836
Gene: ENSMUSG00000000766
AA Change: S14P

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1.7e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1e-10	PFAM
Pfam:7tm_1	85	336	3.4e-67	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000152674
AA Change: S14P

PolyPhen 2 Score 0.980 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000115552
Gene: ENSMUSG00000000766
AA Change: S14P

Domain	Start	End	E-Value	Type
SCOP:d1l9ha_	46	94	8e-8	SMART
PDB:4DKL\|A	52	94	7e-23	PDB

Predicted Effect

probably benign
Transcript: ENSMUST00000154906
AA Change: S14P

PolyPhen 2 Score 0.103 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000114342
Gene: ENSMUSG00000000766
AA Change: S14P

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	332	1.5e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1.1e-10	PFAM
Pfam:7tm_1	85	336	3.6e-67	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000148625

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141897

Predicted Effect

probably benign
Transcript: ENSMUST00000154941

SMART Domains

Protein: ENSMUSP00000115413
Gene: ENSMUSG00000000766

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	12	261	9.6e-8	PFAM
Pfam:7TM_GPCR_Srsx	15	287	6.1e-11	PFAM
Pfam:7tm_1	21	272	2e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000147171

SMART Domains

Protein: ENSMUSP00000117950
Gene: ENSMUSG00000000766

Domain	Start	End	E-Value	Type
Pfam:7tm_1	24	268	9.2e-64	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000150374

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 94.9%

Validation Efficiency

100% (43/43)

MGI Phenotype

FUNCTION: This gene encodes the mu opioid receptor which is where drugs such as morphine and other opioids have pharmacological effects. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]
PHENOTYPE: Homozygotes for null mutations exhibit isoform dependent loss of behavioral and gastrointestinal opioid responses and may also show impaired spatial memory, heightened nociception, reduced locomotor activity, increased hematopoietic proliferation, and decreased male fertility. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610021A01Rik	C	T	7: 41,275,829 (GRCm39)	R511*	probably null	Het
Acox3	T	C	5: 35,762,611 (GRCm39)	I495T	possibly damaging	Het
Acsl3	A	G	1: 78,672,011 (GRCm39)	S302G	probably benign	Het
Ap1s1	T	C	5: 137,066,357 (GRCm39)	D148G	probably damaging	Het
Asprv1	T	A	6: 86,605,348 (GRCm39)	C65S	probably damaging	Het
Cdkal1	A	G	13: 29,628,018 (GRCm39)	S39P	unknown	Het
Chrna2	T	C	14: 66,386,817 (GRCm39)	V321A	possibly damaging	Het
Chsy1	G	A	7: 65,821,912 (GRCm39)	G716R	probably damaging	Het
Col13a1	G	A	10: 61,797,110 (GRCm39)	R106W	probably damaging	Het
Cul9	A	G	17: 46,813,154 (GRCm39)	V2355A	probably damaging	Het
Cwh43	T	C	5: 73,565,679 (GRCm39)		probably benign	Het
Dbi	C	T	1: 120,047,846 (GRCm39)		probably benign	Het
Dync1h1	G	A	12: 110,607,460 (GRCm39)		probably null	Het
Fabp3	C	T	4: 130,206,180 (GRCm39)	T57I	probably benign	Het
Fbxw21	A	G	9: 108,974,578 (GRCm39)	I314T	probably benign	Het
Fstl3	T	C	10: 79,617,057 (GRCm39)	V200A	probably benign	Het
Gpr45	G	A	1: 43,071,668 (GRCm39)	D104N	possibly damaging	Het
Gsdma2	T	C	11: 98,548,085 (GRCm39)	S184P	probably damaging	Het
Gtf2ird1	T	C	5: 134,386,538 (GRCm39)		probably null	Het
H2-T22	A	G	17: 36,351,537 (GRCm39)	L231S	probably damaging	Het
Hrh4	T	C	18: 13,155,426 (GRCm39)	C322R	probably benign	Het
Ing1	T	A	8: 11,611,641 (GRCm39)	S26R	probably benign	Het
Kif3a	A	T	11: 53,469,757 (GRCm39)	I123F	probably damaging	Het
Lrp6	T	C	6: 134,444,489 (GRCm39)	E1127G	probably damaging	Het
Ltf	G	T	9: 110,857,540 (GRCm39)	C443F	possibly damaging	Het
Mdc1	A	T	17: 36,164,529 (GRCm39)	Q1359L	possibly damaging	Het
Mdga1	A	T	17: 30,071,442 (GRCm39)	I393N	probably damaging	Het
Mnd1	C	A	3: 84,041,416 (GRCm39)	C62F	probably benign	Het
Myo3a	T	C	2: 22,345,067 (GRCm39)	V509A	possibly damaging	Het
Nav2	C	T	7: 49,206,780 (GRCm39)	T1535I	probably damaging	Het
Nlrp4d	G	T	7: 10,112,256 (GRCm39)	S626*	probably null	Het
Nup188	T	A	2: 30,215,358 (GRCm39)	I732K	probably damaging	Het
Or1j12	T	C	2: 36,342,779 (GRCm39)	F61L	probably damaging	Het
Or1l8	G	A	2: 36,817,419 (GRCm39)	R236C	probably benign	Het
Or5b101	C	T	19: 13,005,412 (GRCm39)	A94T	probably benign	Het
Pigr	G	A	1: 130,769,272 (GRCm39)	V28M	probably damaging	Het
Pnpla2	C	T	7: 141,038,391 (GRCm39)	L215F	probably damaging	Het
Pth	T	C	7: 112,985,136 (GRCm39)	H79R	probably benign	Het
Rasal1	T	A	5: 120,809,685 (GRCm39)	L530Q	probably damaging	Het
Sdccag8	A	T	1: 176,775,937 (GRCm39)	K616M	possibly damaging	Het
Slc4a5	C	T	6: 83,273,651 (GRCm39)	T997I	probably damaging	Het
Sp110	A	C	1: 85,505,050 (GRCm39)	F434C	probably benign	Het
Trav7d-4	C	T	14: 53,007,584 (GRCm39)	Q26*	probably null	Het
Zcchc8	A	G	5: 123,858,930 (GRCm39)	S22P	probably benign	Het

Other mutations in Oprm1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01018:Oprm1	APN	10	6,987,170 (GRCm39)	utr 3 prime	probably benign
IGL01768:Oprm1	APN	10	6,779,186 (GRCm39)	missense	probably damaging	1.00
IGL02455:Oprm1	APN	10	6,780,219 (GRCm39)	missense	probably damaging	1.00
IGL03391:Oprm1	APN	10	6,964,077 (GRCm39)	intron	probably benign
IGL03410:Oprm1	APN	10	6,780,051 (GRCm39)	missense	probably damaging	1.00
IGL03048:Oprm1	UTSW	10	6,779,064 (GRCm39)	missense	probably damaging	1.00
R0189:Oprm1	UTSW	10	6,739,071 (GRCm39)	missense	possibly damaging	0.94
R0321:Oprm1	UTSW	10	6,779,183 (GRCm39)	missense	probably damaging	1.00
R0629:Oprm1	UTSW	10	6,782,604 (GRCm39)	splice site	probably null
R0730:Oprm1	UTSW	10	6,782,652 (GRCm39)	intron	probably benign
R1542:Oprm1	UTSW	10	6,738,960 (GRCm39)	missense	probably damaging	1.00
R1743:Oprm1	UTSW	10	6,780,105 (GRCm39)	missense	probably damaging	0.99
R1874:Oprm1	UTSW	10	6,739,035 (GRCm39)	missense	probably benign	0.17
R2864:Oprm1	UTSW	10	6,744,226 (GRCm39)	splice site	probably null
R3792:Oprm1	UTSW	10	6,789,544 (GRCm39)	missense	probably benign	0.00
R4008:Oprm1	UTSW	10	6,782,520 (GRCm39)	missense	probably benign
R4049:Oprm1	UTSW	10	6,779,087 (GRCm39)	missense	probably benign	0.36
R4088:Oprm1	UTSW	10	6,780,234 (GRCm39)	missense	probably damaging	1.00
R4724:Oprm1	UTSW	10	6,708,656 (GRCm39)	nonsense	probably null
R4812:Oprm1	UTSW	10	6,782,698 (GRCm39)	intron	probably benign
R4822:Oprm1	UTSW	10	6,779,036 (GRCm39)	missense	probably damaging	0.99
R4855:Oprm1	UTSW	10	6,788,468 (GRCm39)	missense	probably benign	0.01
R5072:Oprm1	UTSW	10	6,782,550 (GRCm39)	missense	probably benign	0.15
R5768:Oprm1	UTSW	10	6,739,026 (GRCm39)	missense	probably damaging	1.00
R5770:Oprm1	UTSW	10	6,739,026 (GRCm39)	missense	probably damaging	1.00
R5995:Oprm1	UTSW	10	6,782,520 (GRCm39)	missense	probably benign
R6327:Oprm1	UTSW	10	6,780,063 (GRCm39)	missense	probably damaging	0.99
R7135:Oprm1	UTSW	10	6,780,203 (GRCm39)	missense	possibly damaging	0.77
R7413:Oprm1	UTSW	10	6,778,919 (GRCm39)	missense	probably damaging	1.00
R7455:Oprm1	UTSW	10	6,780,204 (GRCm39)	missense	probably damaging	1.00
R8192:Oprm1	UTSW	10	6,788,417 (GRCm39)	missense	probably benign	0.04
R8210:Oprm1	UTSW	10	6,780,442 (GRCm39)	missense	probably benign	0.02
R8945:Oprm1	UTSW	10	6,782,644 (GRCm39)	intron	probably benign
R9054:Oprm1	UTSW	10	6,773,914 (GRCm39)	intron	probably benign
R9723:Oprm1	UTSW	10	6,788,514 (GRCm39)	missense	possibly damaging	0.66
R9726:Oprm1	UTSW	10	6,929,694 (GRCm39)	missense	probably benign	0.00
X0066:Oprm1	UTSW	10	6,780,462 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TGGTTCCACTAGGGCTTGTC -3'
(R):5'- GTTTCCAAAGAGGCCCACTAC -3'

Sequencing Primer
(F):5'- CTTGTCCTTGTAAGAAACTGACGGAG -3'
(R):5'- CTACACACACGATAGAATAGAGGGC -3'

Posted On

2014-12-29