Mutagenetix > Incidental Mutation

Incidental Mutation 'R3011:Tlr4'

257503

Institutional Source

Beutler Lab

Gene Symbol

Tlr4

Ensembl Gene

ENSMUSG00000039005

Gene Name

toll-like receptor 4

Synonyms

Lps, lipopolysaccharide response, Rasl2-8

MMRRC Submission

040533-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R3011 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

66745788-66765338 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 66757491 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Stop codon at position 95 (K95*)

Ref Sequence

ENSEMBL: ENSMUSP00000045770 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000048096] [ENSMUST00000107365]

AlphaFold

Q9QUK6

PDB Structure

Crystal structure of mouse TLR4 and mouse MD-2 complex [X-RAY DIFFRACTION]
Crystal structure of mouse TLR4/MD-2/lipid IVa complex [X-RAY DIFFRACTION]
Crystal structure of mouse TLR4/MD-2/LPS complex [X-RAY DIFFRACTION]

Predicted Effect

probably null
Transcript: ENSMUST00000048096
AA Change: K95*

SMART Domains

Protein: ENSMUSP00000045770
Gene: ENSMUSG00000039005
AA Change: K95*

Domain	Start	End	E-Value	Type
LRR	76	99	7.36e0	SMART
LRR	100	123	1.86e0	SMART
LRR	173	196	8.24e0	SMART
LRR	370	401	4.33e1	SMART
LRR	468	492	2.54e2	SMART
LRR	493	516	1.86e2	SMART
LRR	517	540	1.67e2	SMART
LRR	541	563	1.92e2	SMART
LRRCT	576	626	4.74e-3	SMART
transmembrane domain	636	658	N/A	INTRINSIC
TIR	671	816	7.3e-39	SMART
low complexity region	822	833	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000107365

SMART Domains

Protein: ENSMUSP00000102988
Gene: ENSMUSG00000039005

Domain	Start	End	E-Value	Type
PDB:3VQ2\|B	22	86	2e-38	PDB
SCOP:d1m0za_	27	86	4e-6	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000143580

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000147008

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.0%

Validation Efficiency

100% (29/29)

MGI Phenotype

FUNCTION: This gene belongs to the evolutionarily-conserved Toll-like receptor family, whose members are type-1 transmembrane proteins that are involved in innate immunity. Toll-like receptors are characterized by an extracellular leucine-rich repeat domain that functions in ligand recognition and an intracellular toll/interleukin-1 receptor-like domain that is crucial for signal transduction. The receptor encoded by this gene mediates the innate immune response to bacterial lipopolysaccharide, a major component of the outer membrane of Gram-negative bacteria, through synthesis of pro-inflammatory cytokines and chemokines. In addition, this protein can recognize other pathogens from Gram-negative and Gram-positive bacteria as well as viral components. Mice deficient in this gene display a number of immune response-related phenotypes including hyporesponsiveness to bacterial lipopolysaccharide and increased levels of respiratory syncytial virus compared to controls. [provided by RefSeq, Sep 2015]
PHENOTYPE: Homozygotes for spontaneous or targeted mutations are hyporesponsive to bacterial lipopolysaccharide and more susceptible to infection by gram negative bacteria. [provided by MGI curators]

Allele List at MGI

All alleles(10) : Targeted(2) Spontaneous(6) Chemically induced(2)

Other mutations in this stock

Total: 31 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Arhgap31	A	C	16: 38,422,269 (GRCm39)	C1266G	possibly damaging	Het
Brme1	C	A	8: 84,893,539 (GRCm39)	Y235*	probably null	Het
Cers5	A	G	15: 99,670,598 (GRCm39)		probably benign	Het
Clasp2	C	T	9: 113,730,581 (GRCm39)	T905M	probably damaging	Het
Clec16a	A	G	16: 10,428,975 (GRCm39)	N469S	probably benign	Het
Commd3	T	A	2: 18,679,499 (GRCm39)	V128D	probably damaging	Het
Cped1	A	G	6: 22,088,695 (GRCm39)	T253A	probably damaging	Het
Dnajc5b	A	T	3: 19,600,966 (GRCm39)	Y21F	probably damaging	Het
Fhip2a	T	A	19: 57,373,720 (GRCm39)	L660Q	probably damaging	Het
Gm14403	A	G	2: 177,200,786 (GRCm39)	D244G	probably benign	Het
Gm5414	T	C	15: 101,534,047 (GRCm39)	D312G	probably damaging	Het
Ifi204	T	C	1: 173,579,217 (GRCm39)	S543G	probably benign	Het
Itga11	T	A	9: 62,604,262 (GRCm39)	I50N	probably damaging	Het
Lct	C	T	1: 128,229,109 (GRCm39)	V795I	possibly damaging	Het
Lrrc37	T	A	11: 103,503,929 (GRCm39)	T504S	possibly damaging	Het
Map2	A	G	1: 66,453,771 (GRCm39)	D887G	probably damaging	Het
Mgat4e	T	A	1: 134,469,846 (GRCm39)	D66V	possibly damaging	Het
Nqo1	C	T	8: 108,115,743 (GRCm39)	R178H	probably benign	Het
Or13e8	G	T	4: 43,696,624 (GRCm39)	A183E	probably damaging	Het
Or5h22	A	G	16: 58,895,350 (GRCm39)	V31A	probably benign	Het
Otof	G	A	5: 30,540,184 (GRCm39)	A999V	probably damaging	Het
Phf20	A	G	2: 156,129,946 (GRCm39)	D506G	probably benign	Het
Pth2r	C	T	1: 65,376,147 (GRCm39)	H97Y	probably benign	Het
Snx18	G	A	13: 113,753,422 (GRCm39)	Q504*	probably null	Het
Sppl2c	C	T	11: 104,078,141 (GRCm39)	P314S	probably benign	Het
Srgap2	T	A	1: 131,238,329 (GRCm39)	Q520L	probably damaging	Het
Tex11	C	A	X: 99,977,021 (GRCm39)	A487S	possibly damaging	Het
Tmem59l	A	T	8: 70,938,887 (GRCm39)	C96S	probably damaging	Het
Tmtc3	G	T	10: 100,283,444 (GRCm39)	P704T	possibly damaging	Het
Upp2	T	C	2: 58,680,107 (GRCm39)	V293A	probably damaging	Het
Vps72	A	G	3: 95,026,585 (GRCm39)	K177E	probably damaging	Het

Other mutations in Tlr4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01120:Tlr4	APN	4	66,758,662 (GRCm39)	missense	probably benign	0.01
IGL01343:Tlr4	APN	4	66,752,124 (GRCm39)	splice site	probably benign
IGL01669:Tlr4	APN	4	66,759,504 (GRCm39)	missense	possibly damaging	0.48
IGL01875:Tlr4	APN	4	66,757,726 (GRCm39)	missense	probably damaging	1.00
IGL02138:Tlr4	APN	4	66,759,202 (GRCm39)	missense	probably damaging	0.99
IGL02244:Tlr4	APN	4	66,752,298 (GRCm39)	critical splice donor site	probably null
IGL02793:Tlr4	APN	4	66,757,681 (GRCm39)	missense	probably damaging	1.00
IGL03269:Tlr4	APN	4	66,759,033 (GRCm39)	missense	probably damaging	1.00
IGL03288:Tlr4	APN	4	66,757,990 (GRCm39)	missense	probably damaging	0.99
bugsy	UTSW	4	66,757,491 (GRCm39)	nonsense	probably null
Cruyff	UTSW	4	66,758,563 (GRCm39)	missense	probably damaging	1.00
don_knotts	UTSW	4	66,759,409 (GRCm39)	missense	probably damaging	1.00
Guardiola	UTSW	4	66,757,540 (GRCm39)	missense	probably damaging	1.00
Lops	UTSW	4	66,752,117 (GRCm39)	splice site	probably null
lps3	UTSW	4	66,759,334 (GRCm39)	missense	probably damaging	1.00
Lps4	UTSW	4	66,759,379 (GRCm39)	missense	probably damaging	1.00
milquetoast	UTSW	4	66,757,681 (GRCm39)	missense	probably damaging	1.00
salvador	UTSW	4	66,758,443 (GRCm39)	missense	probably damaging	0.99
R0449:Tlr4	UTSW	4	66,757,857 (GRCm39)	missense	probably damaging	0.99
R0481:Tlr4	UTSW	4	66,746,153 (GRCm39)	missense	probably benign	0.05
R0576:Tlr4	UTSW	4	66,757,732 (GRCm39)	missense	probably benign	0.00
R0827:Tlr4	UTSW	4	66,752,117 (GRCm39)	splice site	probably null
R1488:Tlr4	UTSW	4	66,757,786 (GRCm39)	missense	probably damaging	1.00
R1490:Tlr4	UTSW	4	66,757,611 (GRCm39)	missense	possibly damaging	0.56
R1522:Tlr4	UTSW	4	66,757,933 (GRCm39)	missense	possibly damaging	0.80
R1616:Tlr4	UTSW	4	66,757,717 (GRCm39)	missense	probably damaging	1.00
R1681:Tlr4	UTSW	4	66,759,342 (GRCm39)	missense	probably damaging	1.00
R1738:Tlr4	UTSW	4	66,759,313 (GRCm39)	missense	probably benign	0.19
R1888:Tlr4	UTSW	4	66,759,409 (GRCm39)	missense	probably damaging	1.00
R1888:Tlr4	UTSW	4	66,759,409 (GRCm39)	missense	probably damaging	1.00
R1929:Tlr4	UTSW	4	66,757,681 (GRCm39)	missense	probably damaging	1.00
R1982:Tlr4	UTSW	4	66,759,272 (GRCm39)	missense	probably benign	0.40
R1998:Tlr4	UTSW	4	66,758,707 (GRCm39)	missense	probably damaging	1.00
R2186:Tlr4	UTSW	4	66,758,220 (GRCm39)	missense	possibly damaging	0.63
R2305:Tlr4	UTSW	4	66,758,338 (GRCm39)	missense	probably damaging	1.00
R3420:Tlr4	UTSW	4	66,757,773 (GRCm39)	missense	probably benign	0.37
R3422:Tlr4	UTSW	4	66,757,773 (GRCm39)	missense	probably benign	0.37
R3818:Tlr4	UTSW	4	66,759,553 (GRCm39)	missense	probably benign	0.00
R4212:Tlr4	UTSW	4	66,758,563 (GRCm39)	missense	probably damaging	1.00
R4213:Tlr4	UTSW	4	66,758,563 (GRCm39)	missense	probably damaging	1.00
R4417:Tlr4	UTSW	4	66,757,540 (GRCm39)	missense	probably damaging	1.00
R4630:Tlr4	UTSW	4	66,757,477 (GRCm39)	missense	probably benign	0.44
R4735:Tlr4	UTSW	4	66,759,435 (GRCm39)	missense	probably damaging	1.00
R5191:Tlr4	UTSW	4	66,759,616 (GRCm39)	missense	probably damaging	0.96
R5613:Tlr4	UTSW	4	66,759,122 (GRCm39)	missense	possibly damaging	0.94
R5705:Tlr4	UTSW	4	66,752,217 (GRCm39)	missense	probably damaging	1.00
R5726:Tlr4	UTSW	4	66,758,652 (GRCm39)	missense	probably benign
R6021:Tlr4	UTSW	4	66,759,103 (GRCm39)	missense	probably damaging	1.00
R6159:Tlr4	UTSW	4	66,758,070 (GRCm39)	missense	possibly damaging	0.92
R6227:Tlr4	UTSW	4	66,758,832 (GRCm39)	missense	probably benign
R7139:Tlr4	UTSW	4	66,758,520 (GRCm39)	missense	probably benign	0.06
R7199:Tlr4	UTSW	4	66,759,430 (GRCm39)	missense	probably damaging	0.99
R7220:Tlr4	UTSW	4	66,758,188 (GRCm39)	missense	probably benign
R7337:Tlr4	UTSW	4	66,758,191 (GRCm39)	missense	possibly damaging	0.86
R7487:Tlr4	UTSW	4	66,842,659 (GRCm39)	missense	probably benign	0.00
R7638:Tlr4	UTSW	4	66,758,443 (GRCm39)	missense	probably damaging	0.99
R7773:Tlr4	UTSW	4	66,757,836 (GRCm39)	missense	probably damaging	1.00
R7814:Tlr4	UTSW	4	66,759,316 (GRCm39)	missense	probably damaging	1.00
R7897:Tlr4	UTSW	4	66,758,058 (GRCm39)	missense	probably benign	0.07
R8044:Tlr4	UTSW	4	66,746,084 (GRCm39)	missense	probably benign	0.01
R8062:Tlr4	UTSW	4	66,758,087 (GRCm39)	missense	probably benign	0.00
R8080:Tlr4	UTSW	4	66,757,713 (GRCm39)	missense	probably damaging	1.00
R8446:Tlr4	UTSW	4	66,757,673 (GRCm39)	missense	probably damaging	0.98
R8916:Tlr4	UTSW	4	66,847,268 (GRCm39)	missense	probably benign	0.06
R9100:Tlr4	UTSW	4	66,758,518 (GRCm39)	missense	probably benign	0.08
R9415:Tlr4	UTSW	4	66,746,160 (GRCm39)	critical splice donor site	probably null
R9562:Tlr4	UTSW	4	66,759,522 (GRCm39)	missense	possibly damaging	0.80
R9565:Tlr4	UTSW	4	66,759,522 (GRCm39)	missense	possibly damaging	0.80
R9752:Tlr4	UTSW	4	66,757,912 (GRCm39)	missense	probably benign	0.02
X0064:Tlr4	UTSW	4	66,758,377 (GRCm39)	missense	probably damaging	0.99
Z1088:Tlr4	UTSW	4	66,847,319 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- AAATCTGCAGAGTTCCTCTCC -3'
(R):5'- GTGAGCCACATTGAGTTTCTTTAAG -3'

Sequencing Primer
(F):5'- TGCTCACACCATCATCACCTG -3'
(R):5'- TAAGGTTATAAGCTGTCCAATAGGG -3'

Posted On

2015-01-11