Mutagenetix > Incidental Mutation

Incidental Mutation 'R3159:Cdk15'

258046

Institutional Source

Beutler Lab

Gene Symbol

Cdk15

Ensembl Gene

ENSMUSG00000026023

Gene Name

cyclin dependent kinase 15

Synonyms

Pftk2, Als2cr7

MMRRC Submission

040610-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R3159 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

59296029-59391656 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 59340440 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 313 (I313T)

Ref Sequence

ENSEMBL: ENSMUSP00000124680 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000114248] [ENSMUST00000160662]

AlphaFold

Q3V3A1

Predicted Effect

probably damaging
Transcript: ENSMUST00000114248
AA Change: I311T

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000109886
Gene: ENSMUSG00000026023
AA Change: I311T

Domain	Start	End	E-Value	Type
S_TKc	101	385	7.9e-86	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000160662
AA Change: I313T

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000124680
Gene: ENSMUSG00000026023
AA Change: I313T

Domain	Start	End	E-Value	Type
S_TKc	103	387	7.9e-86	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000162906

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 94.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Bltp1	T	C	3: 37,013,564 (GRCm39)	V1931A	probably benign	Het
Ccdc146	T	C	5: 21,604,790 (GRCm39)	E16G	unknown	Het
Ccr4	G	T	9: 114,321,350 (GRCm39)	N238K	probably benign	Het
Celsr3	T	C	9: 108,704,909 (GRCm39)	V464A	possibly damaging	Het
Cfap54	T	C	10: 92,834,918 (GRCm39)	I1096V	probably benign	Het
Dmpk	A	G	7: 18,826,944 (GRCm39)	T579A	probably benign	Het
Dscam	T	C	16: 96,479,710 (GRCm39)	T1146A	probably benign	Het
Galnt12	A	G	4: 47,104,264 (GRCm39)	D174G	probably damaging	Het
Gm5145	A	G	17: 20,791,155 (GRCm39)	I178V	probably benign	Het
Gxylt1	A	G	15: 93,142,913 (GRCm39)	I384T	probably benign	Het
Hmgcr	C	T	13: 96,802,355 (GRCm39)	V110I	probably damaging	Het
Hsd17b2	A	C	8: 118,485,491 (GRCm39)	D318A	probably damaging	Het
Ighv8-6	A	G	12: 115,129,508 (GRCm39)	S83P	probably damaging	Het
Isg20	C	A	7: 78,564,201 (GRCm39)	A36E	possibly damaging	Het
Jade3	A	G	X: 20,345,783 (GRCm39)	K54E	probably damaging	Het
Mark1	A	T	1: 184,640,584 (GRCm39)	Y505N	probably damaging	Het
Mmp11	C	T	10: 75,762,948 (GRCm39)		probably benign	Het
Myo1g	G	T	11: 6,464,527 (GRCm39)	T511K	possibly damaging	Het
Ociad1	A	T	5: 73,467,688 (GRCm39)	R155*	probably null	Het
Or5t7	A	G	2: 86,506,855 (GRCm39)	I274T	probably benign	Het
Pcdha2	C	T	18: 37,074,250 (GRCm39)	T627I	probably damaging	Het
Polg2	A	C	11: 106,659,163 (GRCm39)	V450G	probably benign	Het
Rbm48	A	T	5: 3,646,105 (GRCm39)	V33D	possibly damaging	Het
Rfx6	A	T	10: 51,602,816 (GRCm39)	R778W	probably damaging	Het
Sav1	A	T	12: 70,031,326 (GRCm39)	D65E	probably benign	Het
Sh3rf1	C	T	8: 61,679,321 (GRCm39)	P121L	probably benign	Het
Shank2	C	A	7: 143,635,611 (GRCm39)	N328K	probably damaging	Het
Slc8a3	C	A	12: 81,361,766 (GRCm39)	R351L	probably damaging	Het
Slc9b1	G	A	3: 135,077,606 (GRCm39)	G100E	probably damaging	Het
Slit1	A	G	19: 41,592,812 (GRCm39)	Y1214H	probably benign	Het
Smu1	T	A	4: 40,754,529 (GRCm39)	R123S	possibly damaging	Het
Tgfb3	A	T	12: 86,105,760 (GRCm39)	W332R	probably damaging	Het
Tmem132a	C	T	19: 10,836,901 (GRCm39)	W680*	probably null	Het
Tns2	C	T	15: 102,017,369 (GRCm39)	R281C	probably damaging	Het
Trav4-4-dv10	A	T	14: 53,921,559 (GRCm39)	K86*	probably null	Het
Zfp667	T	C	7: 6,308,999 (GRCm39)	C556R	probably damaging	Het
Zranb3	A	G	1: 127,900,686 (GRCm39)	I713T	probably benign	Het

Other mutations in Cdk15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01307:Cdk15	APN	1	59,326,955 (GRCm39)	missense	probably benign	0.37
IGL01612:Cdk15	APN	1	59,328,932 (GRCm39)	missense	possibly damaging	0.93
IGL02179:Cdk15	APN	1	59,370,100 (GRCm39)	missense	possibly damaging	0.84
IGL03228:Cdk15	APN	1	59,297,912 (GRCm39)	missense	possibly damaging	0.95
FR4449:Cdk15	UTSW	1	59,296,982 (GRCm39)	small insertion	probably benign
R0270:Cdk15	UTSW	1	59,349,965 (GRCm39)	missense	probably damaging	0.99
R1720:Cdk15	UTSW	1	59,328,917 (GRCm39)	missense	probably damaging	1.00
R1958:Cdk15	UTSW	1	59,383,475 (GRCm39)	missense	probably damaging	0.99
R1969:Cdk15	UTSW	1	59,370,110 (GRCm39)	missense	probably damaging	1.00
R6000:Cdk15	UTSW	1	59,328,818 (GRCm39)	missense	probably damaging	1.00
R6226:Cdk15	UTSW	1	59,304,792 (GRCm39)	missense	probably damaging	1.00
R6257:Cdk15	UTSW	1	59,296,264 (GRCm39)	critical splice donor site	probably null
R7184:Cdk15	UTSW	1	59,304,814 (GRCm39)	missense	probably benign	0.33
R7446:Cdk15	UTSW	1	59,328,854 (GRCm39)	missense	probably damaging	1.00
R7467:Cdk15	UTSW	1	59,328,938 (GRCm39)	missense	probably null	0.96
R7588:Cdk15	UTSW	1	59,383,458 (GRCm39)	missense	possibly damaging	0.95
R8540:Cdk15	UTSW	1	59,349,992 (GRCm39)	missense	possibly damaging	0.93
R9024:Cdk15	UTSW	1	59,326,957 (GRCm39)	missense	probably damaging	1.00
R9372:Cdk15	UTSW	1	59,370,142 (GRCm39)	missense	probably benign	0.00
R9404:Cdk15	UTSW	1	59,328,914 (GRCm39)	missense	possibly damaging	0.55
R9493:Cdk15	UTSW	1	59,326,943 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCCCCAGCGTTTCTCAGAAG -3'
(R):5'- CGTTAGTGGGGAGACTTAATAGGC -3'

Sequencing Primer
(F):5'- CAGCGTTTCTCAGAAGGAGGC -3'
(R):5'- GAGTTTCCTAATGACTCATCCGGAAG -3'

Posted On

2015-01-23