Mutagenetix > Incidental Mutation

Incidental Mutation 'R0328:Vmn2r60'

25858

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r60

Ensembl Gene

ENSMUSG00000090619

Gene Name

vomeronasal 2, receptor 60

Synonyms

Casr-rs3, EG637898, Gprc2a-rs3

MMRRC Submission

038537-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.112) question?

Stock #

R0328 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

41765895-41845200 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

T to C at 41791744 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000128493 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166447]

AlphaFold

A0A3B2WBC8

Predicted Effect

probably benign
Transcript: ENSMUST00000166447

SMART Domains

Protein: ENSMUSP00000128493
Gene: ENSMUSG00000090619

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	78	471	1.2e-44	PFAM
Pfam:NCD3G	514	567	5.1e-23	PFAM
Pfam:7tm_3	600	835	1.4e-51	PFAM

Coding Region Coverage

1x: 98.9%
3x: 97.9%
10x: 95.5%
20x: 90.8%

Validation Efficiency

96% (46/48)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4932414N04Rik	A	T	2: 68,574,624 (GRCm39)	H618L	possibly damaging	Het
Aacs	T	C	5: 125,593,323 (GRCm39)	V642A	probably benign	Het
Alms1	A	G	6: 85,587,796 (GRCm39)		probably null	Het
Arhgap39	A	G	15: 76,636,152 (GRCm39)		probably benign	Het
Bard1	C	T	1: 71,085,921 (GRCm39)	V595I	probably benign	Het
Bptf	T	C	11: 106,937,953 (GRCm39)	K2713E	probably damaging	Het
Calhm1	C	T	19: 47,129,742 (GRCm39)	G260D	possibly damaging	Het
Ccdc154	A	C	17: 25,390,779 (GRCm39)	K643T	probably benign	Het
Ccl4	T	A	11: 83,554,383 (GRCm39)	S59T	probably damaging	Het
Cntd1	T	C	11: 101,174,259 (GRCm39)	S73P	probably benign	Het
Colgalt2	A	T	1: 152,348,859 (GRCm39)	D168V	probably damaging	Het
Fam117a	T	C	11: 95,266,452 (GRCm39)		probably benign	Het
Fat1	A	G	8: 45,476,827 (GRCm39)	T1935A	probably benign	Het
Fbxw21	T	A	9: 108,975,653 (GRCm39)	I248F	possibly damaging	Het
Fhod3	A	T	18: 25,246,657 (GRCm39)	M1288L	probably benign	Het
Gm5114	T	G	7: 39,057,885 (GRCm39)	K578T	probably damaging	Het
Gxylt2	A	T	6: 100,727,496 (GRCm39)		probably benign	Het
Helz	G	T	11: 107,495,174 (GRCm39)	A383S	probably benign	Het
Ift172	C	A	5: 31,421,195 (GRCm39)	E968*	probably null	Het
Itpripl1	T	C	2: 126,983,924 (GRCm39)	N66S	possibly damaging	Het
Kcnma1	A	G	14: 23,423,265 (GRCm39)	Y686H	probably damaging	Het
Ndrg1	C	A	15: 66,815,008 (GRCm39)		probably benign	Het
Ogdh	T	C	11: 6,297,216 (GRCm39)	V545A	probably benign	Het
Or10al5	A	G	17: 38,063,284 (GRCm39)	I180V	possibly damaging	Het
P3h3	G	A	6: 124,831,269 (GRCm39)		probably benign	Het
Ppme1	A	T	7: 99,983,182 (GRCm39)		probably null	Het
Prkag1	T	G	15: 98,713,563 (GRCm39)	D44A	probably damaging	Het
Prpf39	T	C	12: 65,090,145 (GRCm39)		probably benign	Het
Rabep1	C	A	11: 70,810,033 (GRCm39)	R489S	probably damaging	Het
Scn10a	A	G	9: 119,523,168 (GRCm39)	V75A	possibly damaging	Het
Sema3d	T	C	5: 12,498,042 (GRCm39)	L16P	possibly damaging	Het
Skida1	T	C	2: 18,051,997 (GRCm39)		probably benign	Het
Sptbn4	T	C	7: 27,063,595 (GRCm39)	Y2277C	probably damaging	Het
Syne1	A	G	10: 5,298,945 (GRCm39)	I1047T	possibly damaging	Het
Syt17	A	G	7: 117,981,216 (GRCm39)	Y369H	probably benign	Het
Tmem131l	C	T	3: 83,829,238 (GRCm39)		probably benign	Het
Traf3ip2	A	T	10: 39,510,669 (GRCm39)	D314V	probably damaging	Het
Ttc28	T	G	5: 111,431,933 (GRCm39)		probably benign	Het
Ush1c	A	C	7: 45,874,872 (GRCm39)		probably benign	Het
Utp20	A	T	10: 88,602,969 (GRCm39)	Y1884N	possibly damaging	Het
Vmn2r112	T	A	17: 22,824,251 (GRCm39)	I502K	probably benign	Het
Vmn2r63	T	C	7: 42,552,699 (GRCm39)	I852M	probably benign	Het
Vmn2r9	T	A	5: 108,995,405 (GRCm39)	E414D	probably benign	Het
Wnt4	A	G	4: 137,022,754 (GRCm39)	T106A	probably damaging	Het
Zbtb26	A	T	2: 37,326,807 (GRCm39)	N76K	possibly damaging	Het
Zfhx2	T	C	14: 55,309,445 (GRCm39)	T885A	probably benign	Het

Other mutations in Vmn2r60

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01622:Vmn2r60	APN	7	41,785,910 (GRCm39)	missense	probably benign	0.09
IGL01623:Vmn2r60	APN	7	41,785,910 (GRCm39)	missense	probably benign	0.09
IGL02363:Vmn2r60	APN	7	41,844,578 (GRCm39)	missense	probably benign	0.02
IGL02485:Vmn2r60	APN	7	41,844,890 (GRCm39)	missense	possibly damaging	0.54
IGL02651:Vmn2r60	APN	7	41,845,010 (GRCm39)	missense	probably damaging	0.99
IGL02660:Vmn2r60	APN	7	41,791,720 (GRCm39)	nonsense	probably null
IGL03135:Vmn2r60	APN	7	41,786,018 (GRCm39)	missense	probably benign	0.13
IGL03307:Vmn2r60	APN	7	41,765,971 (GRCm39)	missense	probably benign	0.14
R0310:Vmn2r60	UTSW	7	41,844,564 (GRCm39)	missense	possibly damaging	0.54
R0314:Vmn2r60	UTSW	7	41,784,985 (GRCm39)	splice site	probably benign
R0464:Vmn2r60	UTSW	7	41,785,255 (GRCm39)	missense	probably damaging	0.99
R0755:Vmn2r60	UTSW	7	41,844,869 (GRCm39)	missense	probably damaging	1.00
R1119:Vmn2r60	UTSW	7	41,844,365 (GRCm39)	missense	possibly damaging	0.68
R1162:Vmn2r60	UTSW	7	41,845,195 (GRCm39)	missense	probably benign	0.29
R1241:Vmn2r60	UTSW	7	41,786,476 (GRCm39)	missense	probably benign	0.01
R1404:Vmn2r60	UTSW	7	41,786,211 (GRCm39)	missense	probably damaging	0.99
R1404:Vmn2r60	UTSW	7	41,786,211 (GRCm39)	missense	probably damaging	0.99
R1488:Vmn2r60	UTSW	7	41,786,137 (GRCm39)	missense	probably benign	0.17
R1623:Vmn2r60	UTSW	7	41,785,279 (GRCm39)	nonsense	probably null
R1628:Vmn2r60	UTSW	7	41,785,830 (GRCm39)	nonsense	probably null
R1883:Vmn2r60	UTSW	7	41,786,094 (GRCm39)	missense	probably damaging	0.99
R1884:Vmn2r60	UTSW	7	41,786,094 (GRCm39)	missense	probably damaging	0.99
R2182:Vmn2r60	UTSW	7	41,844,931 (GRCm39)	missense	probably benign	0.06
R2275:Vmn2r60	UTSW	7	41,786,251 (GRCm39)	nonsense	probably null
R2847:Vmn2r60	UTSW	7	41,785,857 (GRCm39)	missense	probably benign	0.07
R2885:Vmn2r60	UTSW	7	41,790,403 (GRCm39)	missense	possibly damaging	0.91
R2894:Vmn2r60	UTSW	7	41,785,220 (GRCm39)	missense	probably benign
R2921:Vmn2r60	UTSW	7	41,790,459 (GRCm39)	missense	probably damaging	0.98
R2922:Vmn2r60	UTSW	7	41,790,459 (GRCm39)	missense	probably damaging	0.98
R3772:Vmn2r60	UTSW	7	41,765,980 (GRCm39)	missense	probably benign	0.35
R3820:Vmn2r60	UTSW	7	41,785,125 (GRCm39)	missense	probably damaging	0.98
R3822:Vmn2r60	UTSW	7	41,785,125 (GRCm39)	missense	probably damaging	0.98
R3872:Vmn2r60	UTSW	7	41,785,878 (GRCm39)	missense	probably benign	0.19
R4222:Vmn2r60	UTSW	7	41,765,952 (GRCm39)	missense	probably benign	0.08
R4223:Vmn2r60	UTSW	7	41,765,952 (GRCm39)	missense	probably benign	0.08
R4224:Vmn2r60	UTSW	7	41,765,952 (GRCm39)	missense	probably benign	0.08
R4526:Vmn2r60	UTSW	7	41,844,667 (GRCm39)	missense	probably damaging	0.96
R4547:Vmn2r60	UTSW	7	41,785,087 (GRCm39)	missense	probably null	0.54
R4840:Vmn2r60	UTSW	7	41,785,285 (GRCm39)	missense	probably damaging	1.00
R5173:Vmn2r60	UTSW	7	41,844,935 (GRCm39)	missense	probably damaging	0.97
R5231:Vmn2r60	UTSW	7	41,786,448 (GRCm39)	missense	possibly damaging	0.93
R5480:Vmn2r60	UTSW	7	41,785,154 (GRCm39)	missense	probably damaging	0.98
R5521:Vmn2r60	UTSW	7	41,845,049 (GRCm39)	missense	probably damaging	0.99
R5834:Vmn2r60	UTSW	7	41,765,932 (GRCm39)	missense	probably benign	0.17
R6038:Vmn2r60	UTSW	7	41,844,386 (GRCm39)	missense	probably benign	0.04
R6038:Vmn2r60	UTSW	7	41,844,386 (GRCm39)	missense	probably benign	0.04
R6112:Vmn2r60	UTSW	7	41,844,847 (GRCm39)	missense	probably damaging	1.00
R6149:Vmn2r60	UTSW	7	41,786,400 (GRCm39)	missense	probably damaging	1.00
R6170:Vmn2r60	UTSW	7	41,785,045 (GRCm39)	missense	possibly damaging	0.94
R6383:Vmn2r60	UTSW	7	41,765,895 (GRCm39)	start codon destroyed	probably null	0.04
R6811:Vmn2r60	UTSW	7	41,844,310 (GRCm39)	missense	probably damaging	1.00
R6876:Vmn2r60	UTSW	7	41,785,087 (GRCm39)	missense	probably null	0.54
R6997:Vmn2r60	UTSW	7	41,791,716 (GRCm39)	missense	probably benign	0.00
R7040:Vmn2r60	UTSW	7	41,791,666 (GRCm39)	missense	probably benign	0.00
R7116:Vmn2r60	UTSW	7	41,786,487 (GRCm39)	missense	probably benign	0.00
R7128:Vmn2r60	UTSW	7	41,844,536 (GRCm39)	missense	probably damaging	0.96
R7232:Vmn2r60	UTSW	7	41,786,166 (GRCm39)	missense	possibly damaging	0.83
R7296:Vmn2r60	UTSW	7	41,785,826 (GRCm39)	missense	probably benign	0.01
R7376:Vmn2r60	UTSW	7	41,844,631 (GRCm39)	missense	probably damaging	1.00
R7526:Vmn2r60	UTSW	7	41,845,158 (GRCm39)	frame shift	probably null
R7527:Vmn2r60	UTSW	7	41,845,158 (GRCm39)	frame shift	probably null
R7528:Vmn2r60	UTSW	7	41,845,158 (GRCm39)	frame shift	probably null
R7764:Vmn2r60	UTSW	7	41,844,535 (GRCm39)	missense	probably damaging	0.99
R7843:Vmn2r60	UTSW	7	41,844,511 (GRCm39)	missense	probably benign	0.00
R8080:Vmn2r60	UTSW	7	41,790,521 (GRCm39)	missense	probably benign	0.30
R8290:Vmn2r60	UTSW	7	41,791,690 (GRCm39)	missense	probably damaging	1.00
R8342:Vmn2r60	UTSW	7	41,790,494 (GRCm39)	missense	possibly damaging	0.63
R8362:Vmn2r60	UTSW	7	41,844,954 (GRCm39)	missense	probably damaging	1.00
R8418:Vmn2r60	UTSW	7	41,844,850 (GRCm39)	missense	probably damaging	0.97
R8848:Vmn2r60	UTSW	7	41,786,169 (GRCm39)	missense	probably damaging	1.00
R8860:Vmn2r60	UTSW	7	41,791,654 (GRCm39)	missense	probably damaging	0.99
R8882:Vmn2r60	UTSW	7	41,790,518 (GRCm39)	missense	probably benign	0.00
R8913:Vmn2r60	UTSW	7	41,785,778 (GRCm39)	missense	probably benign	0.27
R9190:Vmn2r60	UTSW	7	41,844,935 (GRCm39)	missense	probably damaging	0.99
R9229:Vmn2r60	UTSW	7	41,791,723 (GRCm39)	missense	possibly damaging	0.95
R9295:Vmn2r60	UTSW	7	41,785,955 (GRCm39)	missense	probably benign	0.01
R9335:Vmn2r60	UTSW	7	41,844,332 (GRCm39)	missense	probably damaging	1.00
R9796:Vmn2r60	UTSW	7	41,785,172 (GRCm39)	missense	probably benign
RF024:Vmn2r60	UTSW	7	41,790,363 (GRCm39)	missense	probably benign	0.01
X0023:Vmn2r60	UTSW	7	41,790,538 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TGGGCCATGTAACCCAAAACTTGAT -3'
(R):5'- CAGACCACTAATTTGTGGGAAAGGTGA -3'

Sequencing Primer
(F):5'- TAGCAATACCAACTCTCATTAGCTTC -3'
(R):5'- TCAGGGGAAAATGTAATCAATGTAAG -3'

Posted On

2013-04-16