Incidental Mutation 'R3402:Tmem74'
ID 259240
Institutional Source Beutler Lab
Gene Symbol Tmem74
Ensembl Gene ENSMUSG00000054409
Gene Name transmembrane protein 74
Synonyms B230382K22Rik
MMRRC Submission 040621-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R3402 (G1)
Quality Score 225
Status Validated
Chromosome 15
Chromosomal Location 43728042-43733432 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) C to T at 43730417 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Valine to Methionine at position 209 (V209M)
Ref Sequence ENSEMBL: ENSMUSP00000070748 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000067469]
AlphaFold Q8BQU7
Predicted Effect probably damaging
Transcript: ENSMUST00000067469
AA Change: V209M

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000070748
Gene: ENSMUSG00000054409
AA Change: V209M

DomainStartEndE-ValueType
low complexity region 60 71 N/A INTRINSIC
Pfam:Neurensin 147 269 1.1e-12 PFAM
Meta Mutation Damage Score 0.6884 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.1%
  • 20x: 94.3%
Validation Efficiency 97% (30/31)
Allele List at MGI
Other mutations in this stock
Total: 29 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adamts3 A T 5: 89,849,592 (GRCm39) F609L probably benign Het
Adgrv1 T C 13: 81,691,661 (GRCm39) N1642S probably damaging Het
Afdn C T 17: 14,104,176 (GRCm39) R1156C probably damaging Het
Ark2c A G 18: 77,652,782 (GRCm39) V6A probably benign Het
Cactin G A 10: 81,161,709 (GRCm39) R747H probably benign Het
Col12a1 T C 9: 79,551,229 (GRCm39) E2129G probably damaging Het
Dennd4c C T 4: 86,692,780 (GRCm39) P97S probably damaging Het
Dnah3 A G 7: 119,566,879 (GRCm39) V2449A probably benign Het
Dysf G A 6: 84,163,491 (GRCm39) probably null Het
Etl4 A T 2: 20,786,693 (GRCm39) H763L probably damaging Het
Hip1r A G 5: 124,135,046 (GRCm39) E394G probably damaging Het
Ift25 A G 4: 107,130,803 (GRCm39) probably null Het
Kat2b C T 17: 53,972,881 (GRCm39) P732S probably damaging Het
Myo16 A G 8: 10,434,719 (GRCm39) N387S probably benign Het
Nlrp4d A T 7: 10,096,781 (GRCm39) N906K probably damaging Het
Or1ad6 A G 11: 50,859,895 (GRCm39) I17V probably benign Het
Or5b120 G A 19: 13,480,312 (GRCm39) A202T probably benign Het
Pkn1 G A 8: 84,396,859 (GRCm39) R926W probably damaging Het
Ppp1r37 C T 7: 19,266,712 (GRCm39) A392T probably damaging Het
Prkcq A G 2: 11,288,660 (GRCm39) T538A possibly damaging Het
Sel1l2 T A 2: 140,082,958 (GRCm39) Y560F probably damaging Het
Slc6a6 A G 6: 91,703,110 (GRCm39) H161R probably benign Het
Slit2 T C 5: 48,440,763 (GRCm39) Y1212H probably damaging Het
Stk3 G T 15: 34,945,144 (GRCm39) probably benign Het
Tcaf3 T C 6: 42,570,787 (GRCm39) S322G probably benign Het
Tma16 C T 8: 66,936,823 (GRCm39) probably null Het
Unc80 A T 1: 66,549,845 (GRCm39) E701V probably damaging Het
Upk3a T C 15: 84,902,384 (GRCm39) probably null Het
Zfp180 G A 7: 23,805,170 (GRCm39) V530I probably benign Het
Other mutations in Tmem74
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01873:Tmem74 APN 15 43,730,782 (GRCm39) missense probably benign 0.05
IGL01985:Tmem74 APN 15 43,730,476 (GRCm39) missense probably damaging 1.00
R1102:Tmem74 UTSW 15 43,730,186 (GRCm39) missense probably benign 0.00
R1782:Tmem74 UTSW 15 43,730,348 (GRCm39) missense probably damaging 1.00
R1851:Tmem74 UTSW 15 43,730,559 (GRCm39) missense probably benign 0.29
R1852:Tmem74 UTSW 15 43,730,559 (GRCm39) missense probably benign 0.29
R2352:Tmem74 UTSW 15 43,730,506 (GRCm39) missense probably damaging 1.00
R3401:Tmem74 UTSW 15 43,730,417 (GRCm39) missense probably damaging 1.00
R3403:Tmem74 UTSW 15 43,730,417 (GRCm39) missense probably damaging 1.00
R4095:Tmem74 UTSW 15 43,730,678 (GRCm39) nonsense probably null
R4607:Tmem74 UTSW 15 43,730,554 (GRCm39) missense probably damaging 0.98
R4608:Tmem74 UTSW 15 43,730,554 (GRCm39) missense probably damaging 0.98
R5218:Tmem74 UTSW 15 43,730,640 (GRCm39) missense possibly damaging 0.52
R5304:Tmem74 UTSW 15 43,730,217 (GRCm39) nonsense probably null
R5375:Tmem74 UTSW 15 43,730,564 (GRCm39) missense possibly damaging 0.77
R6074:Tmem74 UTSW 15 43,730,134 (GRCm39) missense possibly damaging 0.77
R7264:Tmem74 UTSW 15 43,730,864 (GRCm39) missense probably benign 0.00
R7485:Tmem74 UTSW 15 43,730,761 (GRCm39) missense probably benign 0.01
R7909:Tmem74 UTSW 15 43,730,795 (GRCm39) missense probably benign 0.12
R8366:Tmem74 UTSW 15 43,730,315 (GRCm39) missense probably damaging 1.00
R8389:Tmem74 UTSW 15 43,730,315 (GRCm39) missense probably damaging 1.00
R8732:Tmem74 UTSW 15 43,731,456 (GRCm39) splice site probably benign
R8855:Tmem74 UTSW 15 43,730,231 (GRCm39) missense probably damaging 1.00
R8866:Tmem74 UTSW 15 43,730,231 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GCAAATCTATTTCGACGATAGAGC -3'
(R):5'- AGTCAGAGGTCACCATCAGC -3'

Sequencing Primer
(F):5'- ATTTCGACGATAGAGCTCTCC -3'
(R):5'- GGCAGTAATCACCCTGTAGATCTG -3'
Posted On 2015-01-23