Mutagenetix > Incidental Mutation

Incidental Mutation 'R3709:Zfp472'

259515

Institutional Source

Beutler Lab

Gene Symbol

Zfp472

Ensembl Gene

ENSMUSG00000053600

Gene Name

zinc finger protein 472

Synonyms

Krim-1B, Krim-1, Krim-1A

MMRRC Submission

040702-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R3709 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

33184805-33198185 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 33196685 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 253 (Y253*)

Ref Sequence

ENSEMBL: ENSMUSP00000036514 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000039132]

AlphaFold

B0V2W5

Predicted Effect

probably null
Transcript: ENSMUST00000039132
AA Change: Y253*

SMART Domains

Protein: ENSMUSP00000036514
Gene: ENSMUSG00000053600
AA Change: Y253*

Domain	Start	End	E-Value	Type
KRAB	10	62	4.36e-15	SMART
ZnF_C2H2	197	219	2.45e0	SMART
ZnF_C2H2	225	247	2.75e-3	SMART
ZnF_C2H2	253	275	1.76e-1	SMART
ZnF_C2H2	281	303	3.58e-2	SMART
ZnF_C2H2	309	331	3.29e-1	SMART
ZnF_C2H2	337	359	6.08e0	SMART
ZnF_C2H2	365	387	2.32e-1	SMART
ZnF_C2H2	393	415	6.57e-1	SMART
ZnF_C2H2	421	443	1.5e-4	SMART
ZnF_C2H2	449	471	2.2e-2	SMART
ZnF_C2H2	477	499	1.01e-1	SMART
ZnF_C2H2	505	527	8.94e-3	SMART

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 96.9%
20x: 93.9%

Validation Efficiency

98% (50/51)

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9930111J21Rik2	T	G	11: 48,910,480 (GRCm39)	D651A	probably damaging	Het
Abcb1a	A	G	5: 8,788,738 (GRCm39)	N1039S	probably benign	Het
Abcc2	G	T	19: 43,786,885 (GRCm39)	V169F	possibly damaging	Het
Adcy8	T	C	15: 64,597,384 (GRCm39)		probably benign	Het
Aida	C	A	1: 183,085,610 (GRCm39)		probably null	Het
Armc8	A	G	9: 99,402,550 (GRCm39)	I333T	probably damaging	Het
B4galt3	C	A	1: 171,101,613 (GRCm39)	H196N	probably damaging	Het
Ccdc92b	C	A	11: 74,528,933 (GRCm39)	R146S	probably damaging	Het
Cdc42bpa	A	G	1: 179,892,628 (GRCm39)	D264G	probably damaging	Het
Cenpf	A	G	1: 189,381,009 (GRCm39)	S2804P	possibly damaging	Het
Cic	A	G	7: 24,986,406 (GRCm39)	D1276G	probably damaging	Het
Cldn19	T	C	4: 119,114,094 (GRCm39)	S79P	possibly damaging	Het
Ctdp1	G	A	18: 80,493,428 (GRCm39)	Q356*	probably null	Het
Cyp2c69	A	G	19: 39,839,667 (GRCm39)		probably benign	Het
Dhrs3	T	C	4: 144,620,281 (GRCm39)		probably null	Het
Fhod3	T	A	18: 25,223,815 (GRCm39)	W1054R	probably damaging	Het
Gfral	G	A	9: 76,100,725 (GRCm39)	R238*	probably null	Het
Gm10322	C	A	10: 59,451,941 (GRCm39)	D19E	possibly damaging	Het
Gprc6a	CAAA	CA	10: 51,491,776 (GRCm39)		probably null	Het
Idh3a	T	C	9: 54,493,810 (GRCm39)	S4P	possibly damaging	Het
Il15ra	G	T	2: 11,735,458 (GRCm39)		probably null	Het
Ipo8	A	T	6: 148,707,842 (GRCm39)		probably null	Het
Iqgap1	G	A	7: 80,366,835 (GRCm39)	T1595I	possibly damaging	Het
Kalrn	C	T	16: 34,212,400 (GRCm39)		probably null	Het
Klrb1a	T	C	6: 128,595,466 (GRCm39)	D96G	probably benign	Het
Lrp1b	C	T	2: 40,587,454 (GRCm39)	V165M	unknown	Het
Lrp4	A	C	2: 91,320,811 (GRCm39)	T975P	possibly damaging	Het
Lsm14a	T	A	7: 34,053,204 (GRCm39)	I283F	probably damaging	Het
Mael	T	C	1: 166,066,135 (GRCm39)	D34G	probably damaging	Het
Map2	C	T	1: 66,455,015 (GRCm39)	Q1302*	probably null	Het
Mctp1	T	C	13: 76,972,999 (GRCm39)		probably null	Het
Mlxip	T	C	5: 123,585,537 (GRCm39)	V642A	probably benign	Het
Myh9	T	C	15: 77,657,547 (GRCm39)	E1066G	possibly damaging	Het
Naa35	T	A	13: 59,765,846 (GRCm39)		probably benign	Het
Nacc1	T	A	8: 85,403,828 (GRCm39)	I16F	probably damaging	Het
Ncapd3	T	A	9: 26,963,645 (GRCm39)	N499K	probably benign	Het
Or10ab4	A	G	7: 107,655,004 (GRCm39)	M272V	possibly damaging	Het
Osbpl10	C	A	9: 115,036,655 (GRCm39)	P253Q	probably benign	Het
Ptpn21	G	T	12: 98,654,800 (GRCm39)	S722R	probably benign	Het
Rab44	C	T	17: 29,358,843 (GRCm39)	P344S	probably benign	Het
Rbms2	C	T	10: 127,979,312 (GRCm39)	R139Q	probably damaging	Het
Sh3bp2	T	C	5: 34,709,002 (GRCm39)	Y32H	probably damaging	Het
Slc6a15	A	G	10: 103,229,275 (GRCm39)	I105V	probably benign	Het
Thumpd3	A	G	6: 113,032,652 (GRCm39)	D130G	possibly damaging	Het
Trib1	A	G	15: 59,526,210 (GRCm39)	Y260C	probably damaging	Het
Tsks	G	T	7: 44,601,309 (GRCm39)	R208L	possibly damaging	Het
Ttn	G	T	2: 76,577,585 (GRCm39)	S22690*	probably null	Het
Ttyh2	T	G	11: 114,609,958 (GRCm39)	S510A	possibly damaging	Het
Was	G	T	X: 7,952,927 (GRCm39)	S271R	probably benign	Het
Zfp267	T	A	3: 36,213,725 (GRCm39)	C20S	possibly damaging	Het

Other mutations in Zfp472

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00158:Zfp472	APN	17	33,196,498 (GRCm39)	missense	possibly damaging	0.47
IGL03012:Zfp472	APN	17	33,196,545 (GRCm39)	missense	probably benign	0.18
IGL03184:Zfp472	APN	17	33,196,390 (GRCm39)	nonsense	probably null
IGL03223:Zfp472	APN	17	33,196,248 (GRCm39)	missense	probably benign	0.03
R0421:Zfp472	UTSW	17	33,194,897 (GRCm39)	missense	possibly damaging	0.71
R0463:Zfp472	UTSW	17	33,194,936 (GRCm39)	missense	probably damaging	0.98
R0614:Zfp472	UTSW	17	33,196,908 (GRCm39)	missense	possibly damaging	0.53
R1348:Zfp472	UTSW	17	33,196,794 (GRCm39)	missense	probably benign	0.44
R1557:Zfp472	UTSW	17	33,194,900 (GRCm39)	missense	probably benign	0.32
R1630:Zfp472	UTSW	17	33,196,952 (GRCm39)	nonsense	probably null
R1725:Zfp472	UTSW	17	33,196,311 (GRCm39)	missense	possibly damaging	0.53
R1856:Zfp472	UTSW	17	33,184,887 (GRCm39)	missense	possibly damaging	0.53
R1964:Zfp472	UTSW	17	33,196,848 (GRCm39)	missense	possibly damaging	0.79
R2115:Zfp472	UTSW	17	33,196,988 (GRCm39)	missense	possibly damaging	0.73
R2249:Zfp472	UTSW	17	33,197,109 (GRCm39)	missense	possibly damaging	0.87
R2252:Zfp472	UTSW	17	33,195,257 (GRCm39)	nonsense	probably null
R4119:Zfp472	UTSW	17	33,197,189 (GRCm39)	nonsense	probably null
R4406:Zfp472	UTSW	17	33,197,134 (GRCm39)	missense	probably benign	0.01
R4485:Zfp472	UTSW	17	33,196,542 (GRCm39)	missense	possibly damaging	0.96
R4650:Zfp472	UTSW	17	33,196,631 (GRCm39)	missense	possibly damaging	0.86
R4820:Zfp472	UTSW	17	33,196,416 (GRCm39)	missense	probably benign	0.01
R5369:Zfp472	UTSW	17	33,196,717 (GRCm39)	missense	probably damaging	0.98
R5438:Zfp472	UTSW	17	33,197,193 (GRCm39)	missense	probably damaging	0.96
R5529:Zfp472	UTSW	17	33,197,407 (GRCm39)	missense	possibly damaging	0.92
R5950:Zfp472	UTSW	17	33,196,481 (GRCm39)	missense	possibly damaging	0.53
R6158:Zfp472	UTSW	17	33,197,363 (GRCm39)	nonsense	probably null
R7012:Zfp472	UTSW	17	33,196,220 (GRCm39)	missense	probably benign	0.00
R8108:Zfp472	UTSW	17	33,196,977 (GRCm39)	missense	possibly damaging	0.86
R8290:Zfp472	UTSW	17	33,197,088 (GRCm39)	missense	probably benign
R8905:Zfp472	UTSW	17	33,197,455 (GRCm39)	missense	possibly damaging	0.82
R9747:Zfp472	UTSW	17	33,196,271 (GRCm39)	missense	possibly damaging	0.53

Predicted Primers

PCR Primer
(F):5'- ACCGATCATGATTATGAGGGAGC -3'
(R):5'- AGCTTTTCCGCAATGCTTAC -3'

Sequencing Primer
(F):5'- CATGATTATGAGGGAGCTTACACTG -3'
(R):5'- TGCTTACACACAAAAGGTCTCTCTC -3'

Posted On

2015-01-23