Mutagenetix > Incidental Mutation

Incidental Mutation 'R3710:Vmn2r69'

259547

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r69

Ensembl Gene

ENSMUSG00000091006

Gene Name

vomeronasal 2, receptor 69

Synonyms

MMRRC Submission

040703-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.088) question?

Stock #

R3710 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

85055584-85064884 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 85055601 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 846 (T846S)

Ref Sequence

ENSEMBL: ENSMUSP00000132726 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171213]

AlphaFold

G3XA45

Predicted Effect

probably benign
Transcript: ENSMUST00000171213
AA Change: T846S

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000132726
Gene: ENSMUSG00000091006
AA Change: T846S

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	465	1.3e-28	PFAM
Pfam:NCD3G	507	559	1.8e-20	PFAM
Pfam:7tm_3	592	827	3.2e-54	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000207880

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.1%
20x: 94.4%

Validation Efficiency

98% (55/56)

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adcy8	T	C	15: 64,597,384 (GRCm39)		probably benign	Het
Agbl2	G	A	2: 90,636,152 (GRCm39)	D563N	probably benign	Het
Aida	C	A	1: 183,085,610 (GRCm39)		probably null	Het
Ank1	A	G	8: 23,577,095 (GRCm39)	D200G	probably damaging	Het
Ankrd28	A	G	14: 31,470,808 (GRCm39)		probably benign	Het
Ap3b2	C	T	7: 81,123,598 (GRCm39)		probably benign	Het
Atrnl1	C	A	19: 57,645,546 (GRCm39)	H469N	probably damaging	Het
B4galt3	C	A	1: 171,101,613 (GRCm39)	H196N	probably damaging	Het
Bud23	A	C	5: 135,085,204 (GRCm39)	S41A	possibly damaging	Het
Car12	G	T	9: 66,658,260 (GRCm39)	A21S	probably damaging	Het
Cav3	T	A	6: 112,436,774 (GRCm39)	M1K	probably null	Het
Cdc42bpa	A	G	1: 179,892,628 (GRCm39)	D264G	probably damaging	Het
Cic	A	G	7: 24,986,406 (GRCm39)	D1276G	probably damaging	Het
Col2a1	A	G	15: 97,888,788 (GRCm39)		probably benign	Het
Col9a2	C	G	4: 120,911,455 (GRCm39)	R599G	probably damaging	Het
Csn3	C	A	5: 88,077,882 (GRCm39)	N129K	possibly damaging	Het
Diaph1	C	A	18: 37,978,537 (GRCm39)	G1209W	probably damaging	Het
Dsg2	A	G	18: 20,735,174 (GRCm39)	T1051A	probably damaging	Het
Gm1965	A	T	6: 89,122,407 (GRCm39)		noncoding transcript	Het
Gprc6a	CAAA	CA	10: 51,491,776 (GRCm39)		probably null	Het
Gsto1	T	C	19: 47,847,971 (GRCm39)		probably null	Het
Il15ra	G	T	2: 11,735,458 (GRCm39)		probably null	Het
Ipo8	A	T	6: 148,707,842 (GRCm39)		probably null	Het
Lsm14a	T	A	7: 34,053,204 (GRCm39)	I283F	probably damaging	Het
Mael	T	C	1: 166,066,135 (GRCm39)	D34G	probably damaging	Het
Marchf6	A	T	15: 31,509,972 (GRCm39)		probably benign	Het
Mtmr10	C	A	7: 63,976,433 (GRCm39)	A410D	possibly damaging	Het
Myh9	T	C	15: 77,657,547 (GRCm39)	E1066G	possibly damaging	Het
Nim1k	A	G	13: 120,173,635 (GRCm39)	S420P	probably benign	Het
Nlrp4c	T	A	7: 6,068,627 (GRCm39)	V176E	probably damaging	Het
Ogfod1	A	T	8: 94,784,380 (GRCm39)	K313*	probably null	Het
Or5an1	T	G	19: 12,260,450 (GRCm39)	F13V	probably damaging	Het
Osbpl10	C	A	9: 115,036,655 (GRCm39)	P253Q	probably benign	Het
Otof	A	T	5: 30,542,610 (GRCm39)	M661K	probably benign	Het
Rbms2	C	T	10: 127,979,312 (GRCm39)	R139Q	probably damaging	Het
Rimbp2	G	A	5: 128,866,795 (GRCm39)	T508I	probably benign	Het
Ros1	A	T	10: 52,037,991 (GRCm39)	C393*	probably null	Het
Rps17	T	A	7: 80,994,672 (GRCm39)	T30S	probably benign	Het
Rps3	T	C	7: 99,128,626 (GRCm39)	K197R	probably benign	Het
Samd11	G	A	4: 156,334,952 (GRCm39)	L109F	probably damaging	Het
Smarca2	T	G	19: 26,646,290 (GRCm39)		probably benign	Het
Sprr2g	C	A	3: 92,282,036 (GRCm39)	P30Q	unknown	Het
Spz1	G	A	13: 92,711,631 (GRCm39)	Q282*	probably null	Het
Syne3	A	G	12: 104,909,697 (GRCm39)	L713P	possibly damaging	Het
Tgm1	C	A	14: 55,950,052 (GRCm39)		probably benign	Het
Tomm22	T	C	15: 79,555,419 (GRCm39)	F55L	probably damaging	Het
Tph2	T	A	10: 115,009,963 (GRCm39)	Y199F	probably benign	Het
Vmn2r108	A	T	17: 20,682,932 (GRCm39)	F757L	probably benign	Het
Was	G	T	X: 7,952,927 (GRCm39)	S271R	probably benign	Het
Zc3hav1	A	C	6: 38,309,097 (GRCm39)	M575R	probably benign	Het

Other mutations in Vmn2r69

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01019:Vmn2r69	APN	7	85,055,739 (GRCm39)	missense	probably benign
IGL01457:Vmn2r69	APN	7	85,055,836 (GRCm39)	missense	possibly damaging	0.87
IGL01760:Vmn2r69	APN	7	85,056,072 (GRCm39)	missense	possibly damaging	0.90
IGL01834:Vmn2r69	APN	7	85,061,576 (GRCm39)	missense	probably damaging	1.00
IGL02001:Vmn2r69	APN	7	85,056,434 (GRCm39)	missense	probably benign	0.05
IGL02057:Vmn2r69	APN	7	85,060,990 (GRCm39)	missense	possibly damaging	0.93
IGL02289:Vmn2r69	APN	7	85,056,054 (GRCm39)	missense	probably damaging	1.00
IGL02472:Vmn2r69	APN	7	85,058,960 (GRCm39)	missense	probably benign	0.01
IGL02478:Vmn2r69	APN	7	85,055,889 (GRCm39)	missense	probably damaging	1.00
IGL02554:Vmn2r69	APN	7	85,059,014 (GRCm39)	missense	probably damaging	1.00
IGL02723:Vmn2r69	APN	7	85,059,416 (GRCm39)	missense	probably damaging	1.00
R0526:Vmn2r69	UTSW	7	85,060,711 (GRCm39)	missense	probably damaging	1.00
R0560:Vmn2r69	UTSW	7	85,058,922 (GRCm39)	critical splice donor site	probably null
R0909:Vmn2r69	UTSW	7	85,055,873 (GRCm39)	missense	probably benign	0.00
R0976:Vmn2r69	UTSW	7	85,056,108 (GRCm39)	missense	probably damaging	1.00
R1158:Vmn2r69	UTSW	7	85,059,058 (GRCm39)	splice site	probably benign
R1459:Vmn2r69	UTSW	7	85,055,908 (GRCm39)	nonsense	probably null
R1482:Vmn2r69	UTSW	7	85,056,082 (GRCm39)	missense	probably damaging	1.00
R1917:Vmn2r69	UTSW	7	85,060,891 (GRCm39)	missense	probably damaging	1.00
R2016:Vmn2r69	UTSW	7	85,056,493 (GRCm39)	missense	probably damaging	0.98
R2108:Vmn2r69	UTSW	7	85,059,404 (GRCm39)	missense	probably benign
R2571:Vmn2r69	UTSW	7	85,064,764 (GRCm39)	missense	probably benign
R2910:Vmn2r69	UTSW	7	85,055,918 (GRCm39)	missense	probably damaging	1.00
R2920:Vmn2r69	UTSW	7	85,060,973 (GRCm39)	missense	probably benign	0.08
R3708:Vmn2r69	UTSW	7	85,061,029 (GRCm39)	missense	probably damaging	0.98
R4757:Vmn2r69	UTSW	7	85,061,575 (GRCm39)	missense	probably damaging	0.99
R4823:Vmn2r69	UTSW	7	85,060,508 (GRCm39)	missense	probably benign	0.21
R4870:Vmn2r69	UTSW	7	85,060,793 (GRCm39)	missense	possibly damaging	0.93
R4918:Vmn2r69	UTSW	7	85,055,967 (GRCm39)	missense	probably benign	0.06
R5022:Vmn2r69	UTSW	7	85,060,367 (GRCm39)	missense	possibly damaging	0.72
R5174:Vmn2r69	UTSW	7	85,064,739 (GRCm39)	missense	possibly damaging	0.92
R5200:Vmn2r69	UTSW	7	85,055,717 (GRCm39)	missense	probably damaging	1.00
R5278:Vmn2r69	UTSW	7	85,060,991 (GRCm39)	missense	probably benign	0.02
R5643:Vmn2r69	UTSW	7	85,056,404 (GRCm39)	missense	probably damaging	0.98
R5996:Vmn2r69	UTSW	7	85,061,117 (GRCm39)	splice site	probably null
R6083:Vmn2r69	UTSW	7	85,055,711 (GRCm39)	missense	probably damaging	1.00
R6140:Vmn2r69	UTSW	7	85,060,657 (GRCm39)	missense	probably damaging	0.99
R6306:Vmn2r69	UTSW	7	85,064,799 (GRCm39)	missense	probably benign	0.04
R6330:Vmn2r69	UTSW	7	85,060,835 (GRCm39)	missense	probably benign
R6380:Vmn2r69	UTSW	7	85,061,067 (GRCm39)	missense	probably benign
R6466:Vmn2r69	UTSW	7	85,056,378 (GRCm39)	missense	probably benign	0.01
R6542:Vmn2r69	UTSW	7	85,060,413 (GRCm39)	nonsense	probably null
R6583:Vmn2r69	UTSW	7	85,059,017 (GRCm39)	missense	probably benign
R6623:Vmn2r69	UTSW	7	85,056,309 (GRCm39)	missense	possibly damaging	0.84
R6709:Vmn2r69	UTSW	7	85,061,069 (GRCm39)	missense	probably benign	0.03
R6732:Vmn2r69	UTSW	7	85,060,351 (GRCm39)	missense	probably benign	0.00
R6741:Vmn2r69	UTSW	7	85,061,724 (GRCm39)	missense	probably benign	0.01
R7070:Vmn2r69	UTSW	7	85,060,688 (GRCm39)	missense	probably damaging	0.98
R7234:Vmn2r69	UTSW	7	85,056,315 (GRCm39)	missense	probably benign	0.22
R7323:Vmn2r69	UTSW	7	85,060,972 (GRCm39)	missense	possibly damaging	0.95
R7427:Vmn2r69	UTSW	7	85,060,467 (GRCm39)	missense	probably benign	0.28
R7428:Vmn2r69	UTSW	7	85,060,467 (GRCm39)	missense	probably benign	0.28
R7453:Vmn2r69	UTSW	7	85,060,768 (GRCm39)	frame shift	probably null
R7532:Vmn2r69	UTSW	7	85,059,622 (GRCm39)	missense	probably benign	0.36
R7556:Vmn2r69	UTSW	7	85,060,768 (GRCm39)	frame shift	probably null
R7562:Vmn2r69	UTSW	7	85,056,420 (GRCm39)	missense	probably benign
R7592:Vmn2r69	UTSW	7	85,060,768 (GRCm39)	frame shift	probably null
R7708:Vmn2r69	UTSW	7	85,061,755 (GRCm39)	missense	possibly damaging	0.87
R7803:Vmn2r69	UTSW	7	85,056,324 (GRCm39)	missense	probably benign	0.00
R7960:Vmn2r69	UTSW	7	85,055,973 (GRCm39)	missense	probably benign
R7966:Vmn2r69	UTSW	7	85,060,762 (GRCm39)	missense	possibly damaging	0.81
R8071:Vmn2r69	UTSW	7	85,055,713 (GRCm39)	nonsense	probably null
R8237:Vmn2r69	UTSW	7	85,060,340 (GRCm39)	missense	probably benign	0.02
R8347:Vmn2r69	UTSW	7	85,064,838 (GRCm39)	missense	probably benign	0.00
R8737:Vmn2r69	UTSW	7	85,055,783 (GRCm39)	missense	probably damaging	1.00
R8795:Vmn2r69	UTSW	7	85,064,883 (GRCm39)	start codon destroyed	probably null	0.94
R8831:Vmn2r69	UTSW	7	85,059,018 (GRCm39)	nonsense	probably null
R8856:Vmn2r69	UTSW	7	85,061,663 (GRCm39)	missense	probably benign	0.00
R8998:Vmn2r69	UTSW	7	85,060,307 (GRCm39)	missense	probably benign	0.33
R8999:Vmn2r69	UTSW	7	85,060,307 (GRCm39)	missense	probably benign	0.33
R9161:Vmn2r69	UTSW	7	85,056,177 (GRCm39)	missense	possibly damaging	0.88
R9228:Vmn2r69	UTSW	7	85,064,697 (GRCm39)	missense	probably benign	0.01
R9494:Vmn2r69	UTSW	7	85,060,768 (GRCm39)	missense	probably damaging	1.00
R9494:Vmn2r69	UTSW	7	85,056,084 (GRCm39)	missense	probably benign	0.08
R9541:Vmn2r69	UTSW	7	85,056,209 (GRCm39)	missense	probably benign
R9620:Vmn2r69	UTSW	7	85,061,504 (GRCm39)	missense	probably benign	0.10
Z1176:Vmn2r69	UTSW	7	85,055,696 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAAGTGGCAATTTTCAGATACCTG -3'
(R):5'- TGACATTCAGCATGCTGGTGTTC -3'

Sequencing Primer
(F):5'- TCCAATAAACTAAAGTCCAGGTTATG -3'
(R):5'- CTGCAGTGTTTGGATGACTTTC -3'

Posted On

2015-01-23