Incidental Mutation 'R2894:Fmo6'
| ID |
260686 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Fmo6
|
| Ensembl Gene |
ENSMUSG00000095576 |
| Gene Name |
flavin containing monooxygenase 6 |
| Synonyms |
|
| MMRRC Submission |
040482-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.068)
|
| Stock # |
R2894 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
1 |
| Chromosomal Location |
162744120-162765084 bp(-) (GRCm39) |
| Type of Mutation |
nonsense |
| DNA Base Change (assembly) |
C to T
at 162750293 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Tryptophan to Stop codon
at position 254
(W254*)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000136378
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000178465]
[ENSMUST00000195576]
|
| AlphaFold |
J3QMN6 |
| Predicted Effect |
probably null
Transcript: ENSMUST00000178465
AA Change: W254*
|
| SMART Domains |
Protein: ENSMUSP00000136378
Gene: ENSMUSG00000095576
AA Change: W254*
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:FMO-like
|
2 |
532 |
3.2e-273 |
PFAM |
|
Pfam:Pyr_redox_2
|
3 |
226 |
2e-13 |
PFAM |
|
Pfam:Pyr_redox_3
|
6 |
220 |
4.6e-17 |
PFAM |
|
Pfam:K_oxygenase
|
79 |
223 |
1e-7 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000195576
|
| SMART Domains |
Protein: ENSMUSP00000144106
Gene: ENSMUSG00000095576
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:FMO-like
|
2 |
118 |
5.1e-58 |
PFAM |
|
Pfam:NAD_binding_8
|
7 |
84 |
2.2e-5 |
PFAM |
|
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.6%
- 10x: 97.3%
- 20x: 95.2%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 34 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| A2ml1 |
C |
T |
6: 128,557,349 (GRCm39) |
A115T |
probably benign |
Het |
| Acox3 |
A |
G |
5: 35,757,192 (GRCm39) |
I344V |
probably benign |
Het |
| Ank2 |
T |
C |
3: 127,041,892 (GRCm39) |
|
probably null |
Het |
| Ccl4 |
C |
A |
11: 83,554,329 (GRCm39) |
|
probably null |
Het |
| Cdh15 |
G |
A |
8: 123,583,374 (GRCm39) |
R59H |
probably benign |
Het |
| Cdk5rap2 |
T |
C |
4: 70,208,110 (GRCm39) |
K779E |
probably benign |
Het |
| Cenpu |
C |
A |
8: 47,029,384 (GRCm39) |
N212K |
probably damaging |
Het |
| Dcun1d2 |
A |
T |
8: 13,328,649 (GRCm39) |
I86N |
probably damaging |
Het |
| Dnah1 |
T |
A |
14: 31,020,718 (GRCm39) |
E1217V |
possibly damaging |
Het |
| Dusp15 |
T |
C |
2: 152,791,005 (GRCm39) |
I31V |
probably benign |
Het |
| Ern2 |
A |
C |
7: 121,780,810 (GRCm39) |
S114A |
possibly damaging |
Het |
| F11 |
A |
G |
8: 45,701,675 (GRCm39) |
S353P |
probably damaging |
Het |
| Gm16494 |
T |
C |
17: 47,327,632 (GRCm39) |
E84G |
unknown |
Het |
| Kdr |
A |
G |
5: 76,107,496 (GRCm39) |
F1016L |
probably damaging |
Het |
| Lrrc37a |
G |
A |
11: 103,388,690 (GRCm39) |
T2245I |
unknown |
Het |
| Mdga1 |
T |
C |
17: 30,071,478 (GRCm39) |
Y381C |
probably damaging |
Het |
| Msh3 |
G |
T |
13: 92,478,868 (GRCm39) |
A367D |
probably benign |
Het |
| Nadk |
A |
G |
4: 155,671,817 (GRCm39) |
N232S |
possibly damaging |
Het |
| Or51e1 |
A |
G |
7: 102,358,882 (GRCm39) |
T139A |
probably damaging |
Het |
| Or7g29 |
A |
T |
9: 19,286,588 (GRCm39) |
Y196* |
probably null |
Het |
| Per2 |
C |
A |
1: 91,373,325 (GRCm39) |
Q154H |
probably damaging |
Het |
| Pik3ap1 |
G |
A |
19: 41,364,500 (GRCm39) |
A73V |
probably benign |
Het |
| Pramel16 |
A |
T |
4: 143,675,692 (GRCm39) |
M378K |
probably damaging |
Het |
| Rad18 |
G |
A |
6: 112,652,734 (GRCm39) |
Q288* |
probably null |
Het |
| Rarb |
T |
C |
14: 16,435,146 (GRCm38) |
D300G |
probably damaging |
Het |
| Ruvbl1 |
C |
T |
6: 88,456,114 (GRCm39) |
R63W |
possibly damaging |
Het |
| Setdb2 |
T |
C |
14: 59,663,916 (GRCm39) |
N77S |
probably benign |
Het |
| Slc22a19 |
C |
A |
19: 7,670,169 (GRCm39) |
K228N |
probably benign |
Het |
| Thoc6 |
A |
G |
17: 23,888,009 (GRCm39) |
S292P |
probably damaging |
Het |
| Tmem126b |
A |
T |
7: 90,120,121 (GRCm39) |
S83R |
probably damaging |
Het |
| Tmem232 |
C |
T |
17: 65,757,408 (GRCm39) |
E262K |
probably damaging |
Het |
| Vmn2r60 |
T |
C |
7: 41,785,220 (GRCm39) |
V144A |
probably benign |
Het |
| Vpreb3 |
C |
T |
10: 75,779,056 (GRCm39) |
|
probably benign |
Het |
| Vwa8 |
A |
G |
14: 79,275,578 (GRCm39) |
N787S |
probably damaging |
Het |
|
| Other mutations in Fmo6 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01392:Fmo6
|
APN |
1 |
162,757,580 (GRCm39) |
nonsense |
probably null |
|
|
IGL02083:Fmo6
|
APN |
1 |
162,748,033 (GRCm39) |
nonsense |
probably null |
|
|
adventure
|
UTSW |
1 |
162,750,379 (GRCm39) |
missense |
probably benign |
|
|
R0792:Fmo6
|
UTSW |
1 |
162,748,132 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R0940:Fmo6
|
UTSW |
1 |
162,753,795 (GRCm39) |
missense |
probably benign |
0.07 |
|
R1173:Fmo6
|
UTSW |
1 |
162,753,710 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1268:Fmo6
|
UTSW |
1 |
162,748,086 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1538:Fmo6
|
UTSW |
1 |
162,753,675 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1694:Fmo6
|
UTSW |
1 |
162,750,241 (GRCm39) |
missense |
probably benign |
|
|
R1717:Fmo6
|
UTSW |
1 |
162,753,821 (GRCm39) |
nonsense |
probably null |
|
|
R1837:Fmo6
|
UTSW |
1 |
162,750,379 (GRCm39) |
missense |
probably benign |
|
|
R2125:Fmo6
|
UTSW |
1 |
162,757,527 (GRCm39) |
missense |
possibly damaging |
0.82 |
|
R2434:Fmo6
|
UTSW |
1 |
162,744,439 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4864:Fmo6
|
UTSW |
1 |
162,751,964 (GRCm39) |
missense |
probably benign |
0.34 |
|
R6414:Fmo6
|
UTSW |
1 |
162,748,014 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R6576:Fmo6
|
UTSW |
1 |
162,750,264 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6883:Fmo6
|
UTSW |
1 |
162,757,461 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7013:Fmo6
|
UTSW |
1 |
162,745,817 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7014:Fmo6
|
UTSW |
1 |
162,753,877 (GRCm39) |
missense |
probably benign |
0.13 |
|
R7657:Fmo6
|
UTSW |
1 |
162,750,285 (GRCm39) |
missense |
probably benign |
|
|
R8351:Fmo6
|
UTSW |
1 |
162,748,174 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8451:Fmo6
|
UTSW |
1 |
162,748,174 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8911:Fmo6
|
UTSW |
1 |
162,748,114 (GRCm39) |
missense |
possibly damaging |
0.80 |
|
R9217:Fmo6
|
UTSW |
1 |
162,748,046 (GRCm39) |
missense |
probably benign |
0.02 |
|
R9274:Fmo6
|
UTSW |
1 |
162,747,921 (GRCm39) |
missense |
probably benign |
0.00 |
|
X0025:Fmo6
|
UTSW |
1 |
162,750,427 (GRCm39) |
missense |
probably damaging |
1.00 |
|
X0025:Fmo6
|
UTSW |
1 |
162,748,065 (GRCm39) |
missense |
probably benign |
0.29 |
|
Z1176:Fmo6
|
UTSW |
1 |
162,753,701 (GRCm39) |
nonsense |
probably null |
|
|
| Predicted Primers |
PCR Primer
(F):5'- TCTAGCCTGCACATGATCTG -3'
(R):5'- CAGAAGAATGCTGAGATCTGTATTGTC -3'
Sequencing Primer
(F):5'- GGACTCTGTCACCTGAGAAATTC -3'
(R):5'- ATGCTGAGATCTGTATTGTCTCTCTC -3'
|
| Posted On |
2015-01-23 |
Incidental Mutation