Mutagenetix > Incidental Mutation

Incidental Mutation 'R2909:Vmn2r87'

261145

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r87

Ensembl Gene

ENSMUSG00000091511

Gene Name

vomeronasal 2, receptor 87

Synonyms

EG625131

MMRRC Submission

040496-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R2909 (G1)

Quality Score

204

Status

Not validated

Chromosome

Chromosomal Location

130307690-130333248 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 130314865 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 240 (N240K)

Ref Sequence

ENSEMBL: ENSMUSP00000129215 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164227]

AlphaFold

E9PZX4

Predicted Effect

probably damaging
Transcript: ENSMUST00000164227
AA Change: N240K

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000129215
Gene: ENSMUSG00000091511
AA Change: N240K

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	77	422	1.8e-27	PFAM
Pfam:NCD3G	508	562	1.8e-19	PFAM
Pfam:7tm_3	595	829	8.8e-55	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.5%
20x: 95.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 36 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actr5	G	A	2: 158,467,140 (GRCm39)	G27R	possibly damaging	Het
Bltp1	A	G	3: 37,002,102 (GRCm39)	D1349G	probably damaging	Het
Chrm3	G	T	13: 9,928,033 (GRCm39)	D334E	probably benign	Het
Clic5	A	C	17: 44,586,146 (GRCm39)	T212P	probably benign	Het
Dapk1	G	A	13: 60,864,631 (GRCm39)		probably null	Het
Dync2h1	A	T	9: 7,049,114 (GRCm39)	L3262H	probably damaging	Het
Epg5	G	C	18: 78,026,691 (GRCm39)	W1227C	probably damaging	Het
Fancm	T	C	12: 65,171,630 (GRCm39)	S1757P	probably damaging	Het
Gm3604	G	C	13: 62,516,832 (GRCm39)	H509D	probably benign	Het
Gramd4	G	T	15: 86,006,384 (GRCm39)	E163*	probably null	Het
Hip1r	T	A	5: 124,138,656 (GRCm39)		probably null	Het
Ice1	G	T	13: 70,744,292 (GRCm39)	T2097K	probably damaging	Het
Il12a	TCAC	TC	3: 68,605,320 (GRCm39)		probably null	Het
Kbtbd7	A	G	14: 79,665,922 (GRCm39)	T585A	probably benign	Het
Kcnd3	C	T	3: 105,566,082 (GRCm39)	A421V	probably damaging	Het
Kcnq3	T	C	15: 65,897,085 (GRCm39)	T272A	possibly damaging	Het
Ly6l	T	G	15: 75,321,481 (GRCm39)		probably null	Het
Minar1	T	C	9: 89,473,331 (GRCm39)	N860S	probably damaging	Het
Mrps11	C	A	7: 78,438,497 (GRCm39)	A83E	probably damaging	Het
Or1af1	C	T	2: 37,110,188 (GRCm39)	P229L	probably damaging	Het
Pax7	T	C	4: 139,556,007 (GRCm39)	I156V	possibly damaging	Het
Plbd1	A	T	6: 136,611,572 (GRCm39)	V235D	probably damaging	Het
Pml	T	C	9: 58,154,526 (GRCm39)	S76G	possibly damaging	Het
Ppp1r42	T	A	1: 10,073,637 (GRCm39)		probably benign	Het
Rsl24d1	T	C	9: 73,029,585 (GRCm39)	L61S	probably damaging	Het
Rtp2	T	C	16: 23,746,235 (GRCm39)	E132G	probably damaging	Het
Sgk1	A	G	10: 21,870,715 (GRCm39)	I23V	probably benign	Het
Sharpin	A	G	15: 76,234,811 (GRCm39)		probably benign	Het
Sipa1l1	T	A	12: 82,404,105 (GRCm39)	Y533N	probably benign	Het
Slc12a9	T	C	5: 137,330,463 (GRCm39)	I81V	probably benign	Het
Stxbp5l	G	A	16: 37,028,548 (GRCm39)	T505M	possibly damaging	Het
Tmem40	A	G	6: 115,713,342 (GRCm39)		probably null	Het
Tnfrsf18	A	T	4: 156,112,727 (GRCm39)	N138Y	probably damaging	Het
Vmn2r91	A	G	17: 18,356,661 (GRCm39)	E776G	probably damaging	Het
Vmn2r92	T	A	17: 18,405,377 (GRCm39)	N840K	possibly damaging	Het
Vmn2r98	T	C	17: 19,287,664 (GRCm39)	I499T	probably damaging	Het

Other mutations in Vmn2r87

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01090:Vmn2r87	APN	10	130,333,247 (GRCm39)	start codon destroyed	probably null	1.00
IGL01295:Vmn2r87	APN	10	130,307,878 (GRCm39)	missense	probably damaging	1.00
IGL01411:Vmn2r87	APN	10	130,308,429 (GRCm39)	missense	probably benign	0.03
IGL01680:Vmn2r87	APN	10	130,315,586 (GRCm39)	nonsense	probably null
IGL01822:Vmn2r87	APN	10	130,307,991 (GRCm39)	missense	probably damaging	1.00
IGL01835:Vmn2r87	APN	10	130,314,978 (GRCm39)	missense	probably damaging	1.00
IGL01965:Vmn2r87	APN	10	130,314,924 (GRCm39)	missense	possibly damaging	0.49
IGL02562:Vmn2r87	APN	10	130,314,513 (GRCm39)	missense	probably damaging	1.00
IGL02665:Vmn2r87	APN	10	130,333,049 (GRCm39)	missense	probably benign	0.16
IGL03202:Vmn2r87	APN	10	130,333,091 (GRCm39)	missense	probably benign
FR4304:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
FR4340:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
FR4342:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
FR4589:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
LCD18:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
R0344:Vmn2r87	UTSW	10	130,315,806 (GRCm39)	missense	probably damaging	1.00
R0374:Vmn2r87	UTSW	10	130,307,848 (GRCm39)	missense	probably damaging	1.00
R0384:Vmn2r87	UTSW	10	130,307,712 (GRCm39)	missense	probably benign
R1144:Vmn2r87	UTSW	10	130,312,098 (GRCm39)	splice site	probably benign
R1172:Vmn2r87	UTSW	10	130,313,453 (GRCm39)	missense	probably benign	0.03
R1860:Vmn2r87	UTSW	10	130,315,755 (GRCm39)	missense	probably benign	0.00
R1866:Vmn2r87	UTSW	10	130,308,441 (GRCm39)	missense	possibly damaging	0.88
R1897:Vmn2r87	UTSW	10	130,307,829 (GRCm39)	missense	probably damaging	1.00
R2360:Vmn2r87	UTSW	10	130,315,631 (GRCm39)	missense	probably damaging	0.99
R3874:Vmn2r87	UTSW	10	130,315,856 (GRCm39)	missense	possibly damaging	0.62
R4113:Vmn2r87	UTSW	10	130,315,691 (GRCm39)	missense	probably benign
R4190:Vmn2r87	UTSW	10	130,308,556 (GRCm39)	missense	probably damaging	1.00
R4197:Vmn2r87	UTSW	10	130,315,779 (GRCm39)	missense	possibly damaging	0.55
R4201:Vmn2r87	UTSW	10	130,308,448 (GRCm39)	missense	probably benign	0.03
R4202:Vmn2r87	UTSW	10	130,308,448 (GRCm39)	missense	probably benign	0.03
R4368:Vmn2r87	UTSW	10	130,315,676 (GRCm39)	missense	probably benign	0.44
R4485:Vmn2r87	UTSW	10	130,315,678 (GRCm39)	nonsense	probably null
R4537:Vmn2r87	UTSW	10	130,308,054 (GRCm39)	missense	probably benign	0.12
R4590:Vmn2r87	UTSW	10	130,315,014 (GRCm39)	missense	possibly damaging	0.69
R4752:Vmn2r87	UTSW	10	130,314,336 (GRCm39)	nonsense	probably null
R4873:Vmn2r87	UTSW	10	130,308,367 (GRCm39)	missense	probably damaging	1.00
R4875:Vmn2r87	UTSW	10	130,308,367 (GRCm39)	missense	probably damaging	1.00
R4923:Vmn2r87	UTSW	10	130,314,435 (GRCm39)	missense	probably damaging	0.99
R4970:Vmn2r87	UTSW	10	130,314,422 (GRCm39)	missense	probably damaging	1.00
R5049:Vmn2r87	UTSW	10	130,308,298 (GRCm39)	missense	probably damaging	0.96
R5112:Vmn2r87	UTSW	10	130,314,422 (GRCm39)	missense	probably damaging	1.00
R5187:Vmn2r87	UTSW	10	130,333,208 (GRCm39)	missense	probably null	0.99
R5618:Vmn2r87	UTSW	10	130,315,817 (GRCm39)	missense	probably damaging	1.00
R6057:Vmn2r87	UTSW	10	130,308,226 (GRCm39)	missense	probably benign	0.02
R6220:Vmn2r87	UTSW	10	130,315,807 (GRCm39)	missense	probably benign	0.01
R6287:Vmn2r87	UTSW	10	130,314,291 (GRCm39)	critical splice donor site	probably null
R6383:Vmn2r87	UTSW	10	130,314,869 (GRCm39)	missense	probably damaging	1.00
R6576:Vmn2r87	UTSW	10	130,314,654 (GRCm39)	missense	probably benign	0.05
R6742:Vmn2r87	UTSW	10	130,308,396 (GRCm39)	missense	probably damaging	1.00
R7086:Vmn2r87	UTSW	10	130,333,178 (GRCm39)	missense	probably benign	0.00
R7162:Vmn2r87	UTSW	10	130,313,416 (GRCm39)	missense	probably benign	0.08
R7419:Vmn2r87	UTSW	10	130,307,992 (GRCm39)	missense	probably damaging	1.00
R7425:Vmn2r87	UTSW	10	130,314,761 (GRCm39)	missense	probably damaging	1.00
R7443:Vmn2r87	UTSW	10	130,308,588 (GRCm39)	missense	probably damaging	1.00
R7571:Vmn2r87	UTSW	10	130,314,940 (GRCm39)	missense	probably damaging	0.99
R7663:Vmn2r87	UTSW	10	130,308,054 (GRCm39)	missense	probably damaging	0.97
R7716:Vmn2r87	UTSW	10	130,308,018 (GRCm39)	missense	probably benign	0.09
R7793:Vmn2r87	UTSW	10	130,313,413 (GRCm39)	missense	probably benign	0.05
R7806:Vmn2r87	UTSW	10	130,315,679 (GRCm39)	missense	probably benign
R7841:Vmn2r87	UTSW	10	130,333,095 (GRCm39)	missense	probably benign	0.31
R8326:Vmn2r87	UTSW	10	130,308,180 (GRCm39)	missense	possibly damaging	0.67
R8411:Vmn2r87	UTSW	10	130,308,126 (GRCm39)	missense	probably damaging	0.99
R8445:Vmn2r87	UTSW	10	130,313,335 (GRCm39)	missense	probably damaging	1.00
R8540:Vmn2r87	UTSW	10	130,314,762 (GRCm39)	missense	possibly damaging	0.74
R8892:Vmn2r87	UTSW	10	130,308,105 (GRCm39)	missense	probably damaging	0.99
R9093:Vmn2r87	UTSW	10	130,308,165 (GRCm39)	missense	probably benign	0.08
R9623:Vmn2r87	UTSW	10	130,315,794 (GRCm39)	missense	probably damaging	1.00
R9667:Vmn2r87	UTSW	10	130,314,776 (GRCm39)	missense	probably damaging	1.00
R9797:Vmn2r87	UTSW	10	130,312,064 (GRCm39)	missense	probably benign	0.44
R9797:Vmn2r87	UTSW	10	130,308,138 (GRCm39)	missense	probably damaging	0.99
Z1088:Vmn2r87	UTSW	10	130,308,183 (GRCm39)	missense	probably damaging	0.98
Z1176:Vmn2r87	UTSW	10	130,307,713 (GRCm39)	missense	probably benign	0.04

Predicted Primers

PCR Primer
(F):5'- TGTGCAAAAGTGACAGTACCATG -3'
(R):5'- CTATGTCCATCAGGTAGCCACC -3'

Sequencing Primer
(F):5'- AACATCCCATTGTGAGGTTGTGATC -3'
(R):5'- GTAGCCACCAAGGACACACATTTG -3'

Posted On

2015-01-23