Mutagenetix > Incidental Mutation

Incidental Mutation 'ANU22:Tas2r116'

262582

Institutional Source

Beutler Lab

Gene Symbol

Tas2r116

Ensembl Gene

ENSMUSG00000030194

Gene Name

taste receptor, type 2, member 116

Synonyms

mGR16, TRB1, Tas2r7, Tas2r16, T2R16, mt2r56, Tas2r14, TRB4

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

ANU22

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

132832401-132833318 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 132832406 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 2 (N2K)

Ref Sequence

ENSEMBL: ENSMUSP00000032315 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032315]

AlphaFold

Q7M713

Predicted Effect

probably benign
Transcript: ENSMUST00000032315
AA Change: N2K

PolyPhen 2 Score 0.021 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000032315
Gene: ENSMUSG00000030194
AA Change: N2K

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	300	2.8e-89	PFAM

Coding Region Coverage

1x: 98.7%
3x: 98.1%
10x: 96.2%
20x: 92.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aco2	A	G	15: 81,797,915 (GRCm39)	E663G	possibly damaging	Het
Adam29	T	A	8: 56,324,879 (GRCm39)	H525L	probably benign	Het
Adamts2	A	G	11: 50,628,190 (GRCm39)	N297D	probably benign	Het
Adamts6	T	A	13: 104,526,590 (GRCm39)	V506E	probably damaging	Het
Ankfy1	G	A	11: 72,655,617 (GRCm39)	E1101K	probably damaging	Het
Arap3	C	T	18: 38,124,380 (GRCm39)		probably null	Het
Asxl3	T	A	18: 22,649,503 (GRCm39)	H497Q	probably benign	Het
Caln1	A	G	5: 130,698,392 (GRCm39)	E96G	probably damaging	Het
Cdh23	T	C	10: 60,148,403 (GRCm39)	T2653A	probably damaging	Het
Cdk5rap2	T	A	4: 70,298,472 (GRCm39)	I87F	possibly damaging	Het
Crybg3	T	A	16: 59,349,590 (GRCm39)	H934L	probably damaging	Het
Cyp2d22	G	A	15: 82,255,869 (GRCm39)	T461I	probably damaging	Het
Dhcr24	T	C	4: 106,429,475 (GRCm39)	F183L	possibly damaging	Het
Dusp16	A	T	6: 134,695,824 (GRCm39)	S336T	probably benign	Het
F930017D23Rik	T	G	10: 43,480,371 (GRCm39)		noncoding transcript	Het
Fasl	A	G	1: 161,609,407 (GRCm39)	V193A	probably damaging	Het
Fgf5	T	C	5: 98,423,175 (GRCm39)	Y187H	probably damaging	Het
Focad	C	A	4: 88,311,784 (GRCm39)	Q1423K	probably benign	Het
Gabrp	T	C	11: 33,505,055 (GRCm39)	T249A	probably damaging	Het
Galnt5	A	T	2: 57,915,354 (GRCm39)	K637*	probably null	Het
Grm6	A	T	11: 50,750,346 (GRCm39)	D503V	probably benign	Het
Hmg20a	A	T	9: 56,394,934 (GRCm39)	D216V	probably damaging	Het
Lyst	C	T	13: 13,852,641 (GRCm39)	R2214C	probably benign	Het
Micu2	T	A	14: 58,181,082 (GRCm39)	D184V	probably damaging	Het
Nabp2	T	A	10: 128,244,631 (GRCm39)	I52F	probably damaging	Het
Nat8f7	A	T	6: 85,684,570 (GRCm39)	L90*	probably null	Het
Or2z2	A	T	11: 58,346,088 (GRCm39)	M229K	probably damaging	Het
Or4k47	T	C	2: 111,451,546 (GRCm39)	N291S	probably damaging	Het
Pphln1	A	G	15: 93,386,985 (GRCm39)	E273G	probably damaging	Het
Ppil1	C	A	17: 29,482,862 (GRCm39)	V14F	possibly damaging	Het
Ptprd	C	A	4: 76,018,693 (GRCm39)	D694Y	probably damaging	Het
Relt	A	T	7: 100,500,905 (GRCm39)	L28Q	probably damaging	Het
Skic8	C	A	9: 54,635,470 (GRCm39)	V44L	probably damaging	Het
Sptbn4	G	A	7: 27,056,812 (GRCm39)	R2525*	probably null	Het
St8sia5	G	A	18: 77,342,358 (GRCm39)	G320D	probably damaging	Het
Taf2	T	C	15: 54,911,670 (GRCm39)	E582G	probably damaging	Het
Tedc1	C	T	12: 113,126,808 (GRCm39)	R357*	probably null	Het
Tmem168	A	T	6: 13,583,045 (GRCm39)	V612E	probably damaging	Het
Unc79	A	G	12: 102,968,130 (GRCm39)	S119G	probably damaging	Het
Washc5	T	A	15: 59,227,688 (GRCm39)	K425*	probably null	Het
Wtap	G	T	17: 13,186,782 (GRCm39)	T255K	probably benign	Het
Zfp148	T	C	16: 33,277,313 (GRCm39)	V134A	probably benign	Het
Zfp397	C	T	18: 24,093,808 (GRCm39)	S431L	probably damaging	Het

Other mutations in Tas2r116

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01305:Tas2r116	APN	6	132,832,406 (GRCm39)	missense	probably benign	0.02
IGL01656:Tas2r116	APN	6	132,832,396 (GRCm39)	unclassified	probably benign
IGL01970:Tas2r116	APN	6	132,832,632 (GRCm39)	missense	probably benign	0.07
R0490:Tas2r116	UTSW	6	132,832,984 (GRCm39)	missense	probably benign	0.02
R2422:Tas2r116	UTSW	6	132,832,557 (GRCm39)	missense	possibly damaging	0.88
R4013:Tas2r116	UTSW	6	132,833,230 (GRCm39)	missense	probably damaging	1.00
R4516:Tas2r116	UTSW	6	132,833,113 (GRCm39)	missense	probably damaging	1.00
R4745:Tas2r116	UTSW	6	132,832,668 (GRCm39)	missense	probably benign	0.14
R4842:Tas2r116	UTSW	6	132,832,660 (GRCm39)	missense	probably benign	0.00
R7723:Tas2r116	UTSW	6	132,832,867 (GRCm39)	missense	probably benign	0.25
R8439:Tas2r116	UTSW	6	132,832,540 (GRCm39)	missense	probably damaging	0.99
R8551:Tas2r116	UTSW	6	132,832,993 (GRCm39)	missense	probably benign	0.01
R9009:Tas2r116	UTSW	6	132,832,963 (GRCm39)	missense	probably damaging	1.00
R9573:Tas2r116	UTSW	6	132,833,140 (GRCm39)	missense	probably benign	0.04
Z1088:Tas2r116	UTSW	6	132,832,911 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TGTCTCTAGGATGCAGTGTGAAATGC -3'
(R):5'- AGATGCTGAGACTTGTAGCAAACCAG -3'

Sequencing Primer
(F):5'- GCAGTGTGAAATGCAAACTACATC -3'
(R):5'- ATCCATTGTCCTGGGTAAAGCAC -3'

Posted On

2015-02-04