Mutagenetix > Incidental Mutation

Incidental Mutation 'R0294:Or9g4'

26266

Institutional Source

Beutler Lab

Gene Symbol

Or9g4

Ensembl Gene

ENSMUSG00000075211

Gene Name

olfactory receptor family 9 subfamily G member 4

Synonyms

MOR213-4, Olfr1006, GA_x6K02T2Q125-47154544-47153606

MMRRC Submission

038511-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0294 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

85504555-85509085 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 85505060 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 145 (V145A)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099917] [ENSMUST00000216084]

AlphaFold

A2ALD2

Predicted Effect

probably benign
Transcript: ENSMUST00000099917
AA Change: V145A

PolyPhen 2 Score 0.021 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000097501
Gene: ENSMUSG00000075211
AA Change: V145A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	39	315	1.3e-51	PFAM
Pfam:7tm_1	49	298	3.6e-20	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000099919
AA Change: V145A

PolyPhen 2 Score 0.989 (Sensitivity: 0.72; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000097503
Gene: ENSMUSG00000075211
AA Change: V145A

Domain	Start	End	E-Value	Type
Pfam:7tm_1	41	290	3.7e-30	PFAM
Pfam:7tm_4	139	283	3.3e-51	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000216084
AA Change: V145A

PolyPhen 2 Score 0.021 (Sensitivity: 0.95; Specificity: 0.80)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000216207

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 95.4%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930527J03Rik	ACCC	ACC	1: 178,276,503 (GRCm38)		noncoding transcript	Het
Aadat	A	G	8: 60,987,642 (GRCm39)	E319G	possibly damaging	Het
Abca13	A	G	11: 9,219,122 (GRCm39)		probably null	Het
Actl7b	A	T	4: 56,740,848 (GRCm39)	L170Q	possibly damaging	Het
Adam29	C	A	8: 56,326,311 (GRCm39)	V48L	probably benign	Het
Aknad1	A	G	3: 108,682,508 (GRCm39)	Y528C	probably damaging	Het
Alas1	T	A	9: 106,118,455 (GRCm39)	K222N	probably damaging	Het
Aplf	A	G	6: 87,623,227 (GRCm39)	V284A	probably benign	Het
Atp11a	G	A	8: 12,877,524 (GRCm39)	V317M	probably benign	Het
Bub3	A	G	7: 131,169,953 (GRCm39)	E206G	possibly damaging	Het
Cblb	T	G	16: 51,956,187 (GRCm39)	F263L	probably damaging	Het
Ces2h	T	A	8: 105,743,236 (GRCm39)	M157K	probably benign	Het
Cfh	A	G	1: 140,110,999 (GRCm39)	F6L	probably benign	Het
Chst1	G	T	2: 92,443,987 (GRCm39)	R153L	probably damaging	Het
Cimap2	T	C	4: 106,470,361 (GRCm39)	D232G	probably damaging	Het
Cntnap5a	T	A	1: 115,843,046 (GRCm39)	N121K	probably benign	Het
Crybg1	A	T	10: 43,862,372 (GRCm39)	S1467R	probably damaging	Het
Cyp2d22	A	G	15: 82,258,646 (GRCm39)	F72L	possibly damaging	Het
Dmrt2	C	T	19: 25,655,435 (GRCm39)	P345S	probably damaging	Het
Dock8	T	C	19: 25,165,714 (GRCm39)	I1866T	probably damaging	Het
Egfem1	A	G	3: 29,744,270 (GRCm39)	N503S	probably damaging	Het
Ehbp1	T	C	11: 22,045,427 (GRCm39)	D774G	probably benign	Het
Foxp2	C	A	6: 15,376,773 (GRCm39)		probably benign	Het
Gins3	T	C	8: 96,364,547 (GRCm39)	V99A	possibly damaging	Het
Grm1	A	T	10: 10,956,143 (GRCm39)	I47N	probably damaging	Het
H2aj	C	G	6: 136,785,602 (GRCm39)	R89G	probably damaging	Het
Hsdl2	T	A	4: 59,601,408 (GRCm39)	S127T	probably benign	Het
Il5ra	A	T	6: 106,689,362 (GRCm39)	M410K	probably benign	Het
Ints7	A	G	1: 191,344,003 (GRCm39)	S548G	possibly damaging	Het
Kcnt1	A	G	2: 25,778,122 (GRCm39)	E80G	probably damaging	Het
Lgr6	A	G	1: 134,915,629 (GRCm39)	V373A	probably damaging	Het
Lgr6	T	A	1: 135,032,799 (GRCm39)	Q27L	unknown	Het
Map3k14	T	C	11: 103,117,963 (GRCm39)	I610V	possibly damaging	Het
Marf1	C	T	16: 13,960,398 (GRCm39)	A549T	probably damaging	Het
Metap1d	T	A	2: 71,352,889 (GRCm39)	H239Q	probably benign	Het
Mgst2	A	G	3: 51,589,251 (GRCm39)	Y88C	probably damaging	Het
Mroh4	T	C	15: 74,477,998 (GRCm39)	N903D	probably benign	Het
Nbeal2	T	G	9: 110,461,927 (GRCm39)	D1476A	probably damaging	Het
Nlgn1	C	A	3: 26,187,625 (GRCm39)	A87S	probably benign	Het
Nln	C	T	13: 104,189,087 (GRCm39)	G295S	probably damaging	Het
Nnt	T	A	13: 119,472,803 (GRCm39)	Y719F	probably benign	Het
Nnt	T	G	13: 119,474,953 (GRCm39)	I659L	possibly damaging	Het
Or2z8	C	T	8: 72,812,244 (GRCm39)	T240M	probably damaging	Het
Or51b17	A	T	7: 103,542,137 (GRCm39)	H268Q	probably benign	Het
Or52s6	G	A	7: 103,092,291 (GRCm39)	T13I	possibly damaging	Het
Or5p78	A	T	7: 108,212,357 (GRCm39)	Y281F	probably damaging	Het
Otogl	C	T	10: 107,613,089 (GRCm39)	C2041Y	probably damaging	Het
Patj	G	T	4: 98,385,285 (GRCm39)	D300Y	probably damaging	Het
Pkhd1l1	A	T	15: 44,423,831 (GRCm39)	E3124D	probably benign	Het
Plbd2	A	G	5: 120,625,514 (GRCm39)		probably null	Het
Pphln1	T	C	15: 93,318,171 (GRCm39)	Y57H	probably damaging	Het
Ppp1r16b	C	T	2: 158,588,523 (GRCm39)	T78M	probably damaging	Het
Prss40	T	G	1: 34,595,162 (GRCm39)	D224A	possibly damaging	Het
Senp6	A	G	9: 80,021,007 (GRCm39)		probably null	Het
Shank3	A	G	15: 89,416,301 (GRCm39)	E666G	probably damaging	Het
Slc13a1	T	A	6: 24,090,779 (GRCm39)	I547F	possibly damaging	Het
Slc17a3	C	T	13: 24,039,841 (GRCm39)	S293F	probably damaging	Het
Slc22a18	G	A	7: 143,046,578 (GRCm39)		probably null	Het
Slc5a4b	A	G	10: 75,917,161 (GRCm39)	C292R	probably damaging	Het
Spata31e5	T	C	1: 28,817,744 (GRCm39)	Q96R	probably benign	Het
Sphkap	T	A	1: 83,255,966 (GRCm39)	E594D	possibly damaging	Het
Srpra	T	A	9: 35,126,811 (GRCm39)	M61K	probably damaging	Het
Trmt10c	A	T	16: 55,855,240 (GRCm39)	Y132N	possibly damaging	Het

Other mutations in Or9g4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01139:Or9g4	APN	2	85,504,841 (GRCm39)	missense	probably damaging	1.00
IGL01520:Or9g4	APN	2	85,504,701 (GRCm39)	missense	probably benign	0.00
IGL01939:Or9g4	APN	2	85,505,285 (GRCm39)	missense	probably damaging	1.00
IGL02060:Or9g4	APN	2	85,505,178 (GRCm39)	missense	probably benign	0.34
IGL02171:Or9g4	APN	2	85,505,285 (GRCm39)	missense	probably damaging	1.00
IGL03058:Or9g4	APN	2	85,505,025 (GRCm39)	missense	probably benign	0.00
IGL03210:Or9g4	APN	2	85,504,697 (GRCm39)	missense	probably damaging	1.00
BB002:Or9g4	UTSW	2	85,504,907 (GRCm39)	missense
BB012:Or9g4	UTSW	2	85,504,907 (GRCm39)	missense
R1304:Or9g4	UTSW	2	85,504,682 (GRCm39)	missense	probably damaging	1.00
R1476:Or9g4	UTSW	2	85,505,262 (GRCm39)	missense	possibly damaging	0.92
R4757:Or9g4	UTSW	2	85,504,664 (GRCm39)	missense	probably damaging	1.00
R4793:Or9g4	UTSW	2	85,504,842 (GRCm39)	missense	probably damaging	1.00
R5804:Or9g4	UTSW	2	85,504,682 (GRCm39)	missense	probably damaging	1.00
R6146:Or9g4	UTSW	2	85,504,938 (GRCm39)	nonsense	probably null
R6511:Or9g4	UTSW	2	85,505,184 (GRCm39)	missense	possibly damaging	0.61
R6896:Or9g4	UTSW	2	85,505,277 (GRCm39)	missense	probably damaging	0.97
R7075:Or9g4	UTSW	2	85,505,168 (GRCm39)	missense
R7344:Or9g4	UTSW	2	85,505,275 (GRCm39)	nonsense	probably null
R7350:Or9g4	UTSW	2	85,505,189 (GRCm39)	missense
R7925:Or9g4	UTSW	2	85,504,907 (GRCm39)	missense
R8704:Or9g4	UTSW	2	85,504,562 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- AATGCTGGAGAGTACCGTGAAGCC -3'
(R):5'- GGAGTTGGACAATCGTACCATCCTG -3'

Sequencing Primer
(F):5'- GTACCGTGAAGCCCACCAC -3'
(R):5'- GGCCACTTGTATCTCAGAAGAC -3'

Posted On

2013-04-16