Incidental Mutation 'R3119:Rgs10'
ID 263149
Institutional Source Beutler Lab
Gene Symbol Rgs10
Ensembl Gene ENSMUSG00000030844
Gene Name regulator of G-protein signalling 10
Synonyms 2310010N19Rik
MMRRC Submission 040592-MU
Accession Numbers
Essential gene? Possibly non essential (E-score: 0.271) question?
Stock # R3119 (G1)
Quality Score 225
Status Not validated
Chromosome 7
Chromosomal Location 127975345-128020482 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 128004955 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Glutamic Acid to Glycine at position 65 (E65G)
Ref Sequence ENSEMBL: ENSMUSP00000146128 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000033133] [ENSMUST00000145739] [ENSMUST00000147840]
AlphaFold Q9CQE5
Predicted Effect probably benign
Transcript: ENSMUST00000033133
AA Change: E65G

PolyPhen 2 Score 0.259 (Sensitivity: 0.91; Specificity: 0.88)
SMART Domains Protein: ENSMUSP00000033133
Gene: ENSMUSG00000030844
AA Change: E65G

DomainStartEndE-ValueType
low complexity region 16 31 N/A INTRINSIC
RGS 41 156 7.05e-46 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000129120
Predicted Effect probably damaging
Transcript: ENSMUST00000145739
AA Change: E65G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
Predicted Effect probably damaging
Transcript: ENSMUST00000147840
AA Change: E69G

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000122030
Gene: ENSMUSG00000030844
AA Change: E69G

DomainStartEndE-ValueType
low complexity region 24 32 N/A INTRINSIC
Pfam:RGS 45 85 1.5e-15 PFAM
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.2%
  • 20x: 94.6%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 10 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. This protein associates specifically with the activated forms of the two related G-protein subunits, G-alphai3 and G-alphaz but fails to interact with the structurally and functionally distinct G-alpha subunits. Regulator of G protein signaling 10 protein is localized in the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: A gene trap mutation of this gene results in impaired glucose tolerance and increased fasting glucose levels whereas a targeted knockout shows defects in osteoclast differentiation and in the skeleton. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 20 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Atp1a4 A G 1: 172,067,393 (GRCm39) F580L probably damaging Het
AW551984 T C 9: 39,504,656 (GRCm39) T532A probably benign Het
Cstf1 A G 2: 172,214,990 (GRCm39) E37G possibly damaging Het
Cubn A C 2: 13,362,973 (GRCm39) F1679L possibly damaging Het
Dqx1 C A 6: 83,043,216 (GRCm39) S651* probably null Het
Fhad1 CG C 4: 141,645,618 (GRCm39) probably null Het
Gata3 T C 2: 9,882,396 (GRCm39) probably null Het
L3mbtl4 G A 17: 68,732,669 (GRCm39) E50K probably benign Het
Man1a T A 10: 53,906,890 (GRCm39) M295L probably damaging Het
Mbip A G 12: 56,392,488 (GRCm39) V33A probably benign Het
Mrpl9 A T 3: 94,355,097 (GRCm39) N223I probably damaging Het
Nrxn1 G A 17: 90,904,947 (GRCm39) Q219* probably null Het
Or8g51 T A 9: 38,608,955 (GRCm39) K236* probably null Het
Prss12 A T 3: 123,298,976 (GRCm39) T583S possibly damaging Het
Spata31e3 A T 13: 50,401,005 (GRCm39) Y440* probably null Het
Syne2 T A 12: 75,956,058 (GRCm39) M588K probably benign Het
Tmem135 A G 7: 88,797,005 (GRCm39) S364P probably benign Het
Ttc41 T C 10: 86,560,184 (GRCm39) M369T possibly damaging Het
Vwa3b G A 1: 37,148,158 (GRCm39) V437I possibly damaging Het
Zfp418 A G 7: 7,184,688 (GRCm39) H217R possibly damaging Het
Other mutations in Rgs10
AlleleSourceChrCoordTypePredicted EffectPPH Score
comptroller UTSW 7 127,975,733 (GRCm39) missense probably damaging 1.00
R1682:Rgs10 UTSW 7 127,975,694 (GRCm39) missense probably benign
R1714:Rgs10 UTSW 7 128,004,946 (GRCm39) missense probably damaging 0.98
R1801:Rgs10 UTSW 7 128,006,201 (GRCm39) missense possibly damaging 0.67
R2200:Rgs10 UTSW 7 127,990,761 (GRCm39) missense probably damaging 0.99
R3118:Rgs10 UTSW 7 128,004,955 (GRCm39) missense probably damaging 1.00
R6903:Rgs10 UTSW 7 127,990,797 (GRCm39) missense probably damaging 1.00
R8504:Rgs10 UTSW 7 128,019,793 (GRCm39) missense probably benign 0.38
R8834:Rgs10 UTSW 7 127,990,809 (GRCm39) missense probably damaging 1.00
R9153:Rgs10 UTSW 7 127,975,733 (GRCm39) missense probably damaging 1.00
R9709:Rgs10 UTSW 7 127,975,729 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- CTGGTACCAGTGAGAATGATCTG -3'
(R):5'- TGTGTCTTGCAATGTCAAAACC -3'

Sequencing Primer
(F):5'- CCAGTGAGAATGATCTGGGCAC -3'
(R):5'- GTCTTGCAATGTCAAAACCATTAAGG -3'
Posted On 2015-02-05