Incidental Mutation 'R2968:Dusp1'
ID 263327
Institutional Source Beutler Lab
Gene Symbol Dusp1
Ensembl Gene ENSMUSG00000024190
Gene Name dual specificity phosphatase 1
Synonyms Ptpn16, MKP1, erp, mkp-1, 3CH134
MMRRC Submission 040524-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R2968 (G1)
Quality Score 225
Status Not validated
Chromosome 17
Chromosomal Location 26724565-26727446 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 26726679 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Phenylalanine to Isoleucine at position 128 (F128I)
Ref Sequence ENSEMBL: ENSMUSP00000025025 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000025025]
AlphaFold P28563
Predicted Effect probably damaging
Transcript: ENSMUST00000025025
AA Change: F128I

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000025025
Gene: ENSMUSG00000024190
AA Change: F128I

DomainStartEndE-ValueType
RHOD 10 134 6.41e-16 SMART
DSPc 173 311 2.22e-69 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000126178
Predicted Effect noncoding transcript
Transcript: ENSMUST00000146077
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.3%
  • 20x: 95.2%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous mutant mice were viable, fertile, and showed no apparent morphological defects. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 22 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abcb1b T A 5: 8,911,485 (GRCm39) I1113N probably damaging Het
Arhgap12 A T 18: 6,111,732 (GRCm39) S211T probably damaging Het
Celsr3 G T 9: 108,709,390 (GRCm39) C1412F probably damaging Het
Cnga3 A G 1: 37,300,159 (GRCm39) Y331C probably damaging Het
Cntln T A 4: 84,875,504 (GRCm39) S313T probably benign Het
Ddx54 T A 5: 120,756,694 (GRCm39) D134E probably damaging Het
Dennd4c A G 4: 86,699,881 (GRCm39) D244G possibly damaging Het
Dsg3 A T 18: 20,658,282 (GRCm39) T298S possibly damaging Het
Fam83g T A 11: 61,594,304 (GRCm39) S613T probably damaging Het
Gm10608 CAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA CAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA 9: 118,989,784 (GRCm39) probably benign Het
Grep1 A G 17: 23,934,785 (GRCm39) F149S possibly damaging Het
Ighv8-12 C T 12: 115,611,570 (GRCm39) R118Q probably benign Het
Klk14 G A 7: 43,341,501 (GRCm39) C51Y probably damaging Het
Mrgprb5 T A 7: 47,818,317 (GRCm39) R139S probably damaging Het
Or51b6 T C 7: 103,556,519 (GRCm39) V291A probably benign Het
Pcbp1 C T 6: 86,502,471 (GRCm39) E143K probably damaging Het
Plxna1 A G 6: 89,319,590 (GRCm39) S572P probably damaging Het
Serpina3n C T 12: 104,375,333 (GRCm39) T135M probably benign Het
Spink11 A T 18: 44,328,777 (GRCm39) F24I possibly damaging Het
Tes A G 6: 17,096,233 (GRCm39) T74A probably benign Het
Vipas39 T C 12: 87,289,345 (GRCm39) N373S probably benign Het
Wdr75 A G 1: 45,856,501 (GRCm39) D508G probably damaging Het
Other mutations in Dusp1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01346:Dusp1 APN 17 26,725,295 (GRCm39) missense probably benign 0.05
IGL01362:Dusp1 APN 17 26,725,618 (GRCm39) missense probably benign 0.16
IGL01363:Dusp1 APN 17 26,725,264 (GRCm39) missense probably damaging 0.99
IGL02186:Dusp1 APN 17 26,726,032 (GRCm39) nonsense probably null
R0374:Dusp1 UTSW 17 26,727,143 (GRCm39) missense probably damaging 0.98
R0385:Dusp1 UTSW 17 26,726,670 (GRCm39) missense probably benign 0.00
R1344:Dusp1 UTSW 17 26,727,293 (GRCm39) missense probably benign 0.28
R1418:Dusp1 UTSW 17 26,727,293 (GRCm39) missense probably benign 0.28
R1773:Dusp1 UTSW 17 26,726,081 (GRCm39) missense probably damaging 1.00
R2269:Dusp1 UTSW 17 26,726,093 (GRCm39) missense probably damaging 1.00
R5253:Dusp1 UTSW 17 26,727,191 (GRCm39) missense probably benign 0.01
R6952:Dusp1 UTSW 17 26,726,577 (GRCm39) missense probably benign 0.03
R7909:Dusp1 UTSW 17 26,726,586 (GRCm39) missense probably benign 0.02
R9302:Dusp1 UTSW 17 26,726,148 (GRCm39) missense probably damaging 1.00
Z1177:Dusp1 UTSW 17 26,726,169 (GRCm39) frame shift probably null
Predicted Primers PCR Primer
(F):5'- AATCCTCCTAGACCTCTGCCAG -3'
(R):5'- ACTTATGCCATCAGCTAGGGAAG -3'

Sequencing Primer
(F):5'- AGACCTCTGCCAGCTCTG -3'
(R):5'- TCCCACCTGGAGTATGGG -3'
Posted On 2015-02-05