Mutagenetix > Incidental Mutation

Incidental Mutation 'R2939:Or8g34'

264584

Institutional Source

Beutler Lab

Gene Symbol

Or8g34

Ensembl Gene

ENSMUSG00000094745

Gene Name

olfactory receptor family 8 subfamily G member 34

Synonyms

GA_x6K02T2PVTD-33158015-33158950, MOR171-42, Olfr954, MOR171-53

MMRRC Submission

040516-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.070) question?

Stock #

R2939 (G1)

Quality Score

200

Status

Validated

Chromosome

Chromosomal Location

39372729-39373673 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 39373226 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Isoleucine at position 166 (M166I)

Ref Sequence

ENSEMBL: ENSMUSP00000079205 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080329] [ENSMUST00000214818]

AlphaFold

Q9EQB6

Predicted Effect

probably benign
Transcript: ENSMUST00000080329
AA Change: M166I

PolyPhen 2 Score 0.024 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000079205
Gene: ENSMUSG00000094745
AA Change: M166I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	34	311	2.6e-52	PFAM
Pfam:7TM_GPCR_Srsx	38	264	3e-5	PFAM
Pfam:7tm_1	44	293	1e-21	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000214818
AA Change: M163I

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000219910

Meta Mutation Damage Score

0.0781

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 94.6%

Validation Efficiency

98% (62/63)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actl11	T	C	9: 107,808,409 (GRCm39)	Y911H	possibly damaging	Het
Arhgef26	A	C	3: 62,288,331 (GRCm39)	K467T	possibly damaging	Het
Armcx6	A	T	X: 133,650,625 (GRCm39)	W69R	probably damaging	Het
Asl	T	C	5: 130,042,245 (GRCm39)	Y277C	probably damaging	Het
Atm	T	C	9: 53,406,011 (GRCm39)	Y1219C	probably damaging	Het
Azin2	C	T	4: 128,828,397 (GRCm39)	C270Y	probably benign	Het
Brsk1	A	T	7: 4,711,139 (GRCm39)	I545F	possibly damaging	Het
Carm1	C	A	9: 21,490,692 (GRCm39)		probably null	Het
Cfap410	A	G	10: 77,817,507 (GRCm39)	N78S	probably benign	Het
Cfap69	G	A	5: 5,694,432 (GRCm39)	A143V	probably damaging	Het
Cldn14	T	C	16: 93,716,192 (GRCm39)	K218R	probably damaging	Het
Col5a3	T	C	9: 20,706,954 (GRCm39)	K714R	unknown	Het
Crybg2	A	G	4: 133,809,745 (GRCm39)	H1517R	possibly damaging	Het
Dagla	A	C	19: 10,233,728 (GRCm39)	F382C	probably damaging	Het
Dixdc1	G	A	9: 50,622,259 (GRCm39)	A25V	probably damaging	Het
Dock6	A	G	9: 21,750,496 (GRCm39)	F473L	possibly damaging	Het
Eif5	T	C	12: 111,506,713 (GRCm39)	C102R	probably damaging	Het
Faiml	A	G	9: 99,114,527 (GRCm39)	C121R	probably damaging	Het
Fhdc1	A	G	3: 84,364,577 (GRCm39)	V223A	possibly damaging	Het
Garin4	T	C	1: 190,896,103 (GRCm39)	D180G	possibly damaging	Het
Gpatch8	A	G	11: 102,399,010 (GRCm39)	V74A	unknown	Het
Haghl	A	G	17: 26,004,060 (GRCm39)	V8A	possibly damaging	Het
Ksr1	A	G	11: 78,936,007 (GRCm39)		probably null	Het
Lama5	A	C	2: 179,840,747 (GRCm39)	Y584D	probably damaging	Het
Lgi4	C	T	7: 30,767,253 (GRCm39)	R427*	probably null	Het
Lrriq1	T	A	10: 102,980,750 (GRCm39)	S1462C	probably damaging	Het
Map3k4	C	T	17: 12,480,157 (GRCm39)	E682K	probably damaging	Het
Myo9b	G	A	8: 71,786,981 (GRCm39)	R721Q	probably benign	Het
Nherf1	C	T	11: 115,071,270 (GRCm39)	R335C	probably damaging	Het
Nmnat2	C	A	1: 152,950,474 (GRCm39)	S53Y	probably damaging	Het
Or8b12i	C	A	9: 20,082,061 (GRCm39)	G269W	probably benign	Het
Pcsk7	C	T	9: 45,827,322 (GRCm39)	A363V	probably damaging	Het
Pdrg1	C	T	2: 152,854,355 (GRCm39)	G62R	probably damaging	Het
Pdzd7	A	G	19: 45,033,862 (GRCm39)	I74T	possibly damaging	Het
Plcb4	T	C	2: 135,781,123 (GRCm39)		probably benign	Het
Pramel28	A	G	4: 143,693,247 (GRCm39)	V77A	probably benign	Het
Rnf25	A	G	1: 74,635,047 (GRCm39)	V135A	possibly damaging	Het
Rph3a	A	G	5: 121,118,212 (GRCm39)		probably benign	Het
Rsf1	CG	CGACGGCGGTG	7: 97,229,115 (GRCm39)		probably benign	Het
Senp6	G	A	9: 80,051,124 (GRCm39)	A1134T	probably benign	Het
Slc1a6	G	T	10: 78,650,448 (GRCm39)	*562L	probably null	Het
Slc26a6	G	A	9: 108,734,236 (GRCm39)	V206I	probably benign	Het
Slc45a3	A	G	1: 131,905,637 (GRCm39)	E206G	probably damaging	Het
Smc1a	T	A	X: 150,816,695 (GRCm39)	Y516N	probably damaging	Het
Smpd3	A	G	8: 106,984,039 (GRCm39)	V560A	probably benign	Het
Spata31g1	A	G	4: 42,972,946 (GRCm39)	K760E	probably benign	Het
Ssc4d	T	C	5: 135,994,578 (GRCm39)	T51A	possibly damaging	Het
Suco	A	G	1: 161,676,220 (GRCm39)	I386T	probably damaging	Het
Tecta	A	T	9: 42,289,290 (GRCm39)	M425K	possibly damaging	Het
Tmprss11e	G	A	5: 86,869,266 (GRCm39)	R96W	probably damaging	Het
Trim63	T	C	4: 134,050,308 (GRCm39)		probably benign	Het
Trpv1	A	G	11: 73,145,675 (GRCm39)	K403R	probably damaging	Het
Ttc39d	A	G	17: 80,524,982 (GRCm39)	Y547C	probably damaging	Het
Unc79	C	T	12: 102,957,684 (GRCm39)	T33I	probably damaging	Het
Usp47	G	A	7: 111,681,743 (GRCm39)	S518N	probably damaging	Het
Vmn2r22	T	A	6: 123,614,594 (GRCm39)	D332V	probably damaging	Het
Vmn2r55	A	G	7: 12,385,832 (GRCm39)	L716P	probably damaging	Het
Zfp558	T	C	9: 18,367,924 (GRCm39)	N288S	possibly damaging	Het

Other mutations in Or8g34

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02704:Or8g34	APN	9	39,373,579 (GRCm39)	missense	probably damaging	0.98
IGL02793:Or8g34	APN	9	39,372,802 (GRCm39)	missense	probably benign	0.09
IGL02964:Or8g34	APN	9	39,373,077 (GRCm39)	missense	possibly damaging	0.88
IGL02979:Or8g34	APN	9	39,372,819 (GRCm39)	missense	probably benign	0.02
R0041:Or8g34	UTSW	9	39,372,772 (GRCm39)	missense	probably benign	0.34
R0153:Or8g34	UTSW	9	39,372,967 (GRCm39)	missense	probably damaging	0.99
R0269:Or8g34	UTSW	9	39,373,090 (GRCm39)	missense	probably damaging	1.00
R0426:Or8g34	UTSW	9	39,372,889 (GRCm39)	missense	probably damaging	1.00
R0731:Or8g34	UTSW	9	39,372,828 (GRCm39)	missense	probably damaging	1.00
R1800:Or8g34	UTSW	9	39,373,410 (GRCm39)	missense	probably damaging	1.00
R2340:Or8g34	UTSW	9	39,373,105 (GRCm39)	missense	probably damaging	1.00
R2901:Or8g34	UTSW	9	39,373,234 (GRCm39)	missense	probably damaging	0.97
R2912:Or8g34	UTSW	9	39,373,512 (GRCm39)	missense	probably damaging	1.00
R2940:Or8g34	UTSW	9	39,373,226 (GRCm39)	missense	probably benign	0.02
R3081:Or8g34	UTSW	9	39,373,226 (GRCm39)	missense	probably benign	0.02
R3765:Or8g34	UTSW	9	39,372,920 (GRCm39)	nonsense	probably null
R4450:Or8g34	UTSW	9	39,373,328 (GRCm39)	missense	probably benign	0.00
R4515:Or8g34	UTSW	9	39,373,527 (GRCm39)	nonsense	probably null
R4786:Or8g34	UTSW	9	39,373,137 (GRCm39)	missense	probably benign	0.16
R4961:Or8g34	UTSW	9	39,373,183 (GRCm39)	missense	probably damaging	1.00
R5219:Or8g34	UTSW	9	39,373,563 (GRCm39)	missense	probably benign	0.09
R5602:Or8g34	UTSW	9	39,373,326 (GRCm39)	missense	probably benign	0.00
R5887:Or8g34	UTSW	9	39,372,787 (GRCm39)	missense	probably damaging	1.00
R5950:Or8g34	UTSW	9	39,373,633 (GRCm39)	missense	probably benign	0.01
R6943:Or8g34	UTSW	9	39,373,159 (GRCm39)	missense	probably benign	0.05
R7567:Or8g34	UTSW	9	39,373,173 (GRCm39)	missense	possibly damaging	0.92
R8817:Or8g34	UTSW	9	39,373,387 (GRCm39)	missense	probably damaging	1.00
R9251:Or8g34	UTSW	9	39,373,668 (GRCm39)	missense	probably benign
X0060:Or8g34	UTSW	9	39,373,570 (GRCm39)	missense	probably damaging	1.00
Z1177:Or8g34	UTSW	9	39,372,997 (GRCm39)	frame shift	probably null

Predicted Primers

PCR Primer
(F):5'- TTTTGGCACAGCAGAGAGC -3'
(R):5'- ACTAAAGGCTTTGGACCTGCC -3'

Sequencing Primer
(F):5'- CTACACATTAGCTGCAATGGC -3'
(R):5'- CCTCACTAGAGCTAATGCGGAG -3'

Posted On

2015-02-05