Mutagenetix > Incidental Mutation

Incidental Mutation 'R3054:Gm8444'

265095

Institutional Source

Beutler Lab

Gene Symbol

Gm8444

Ensembl Gene

ENSMUSG00000069439

Gene Name

predicted gene 8444

Synonyms

MMRRC Submission

040563-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.423) question?

Stock #

R3054 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

81727547-81727914 bp(-) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

A to T at 81727845 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000061390 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000050467] [ENSMUST00000092020]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000050467

SMART Domains

Protein: ENSMUSP00000061390
Gene: ENSMUSG00000048546

Domain	Start	End	E-Value	Type
btg1	1	106	3.69e-64	SMART
Pfam:PAM2	128	145	1e-7	PFAM
low complexity region	175	189	N/A	INTRINSIC
low complexity region	194	218	N/A	INTRINSIC
low complexity region	226	243	N/A	INTRINSIC
Pfam:PAM2	249	266	5.9e-9	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000092020

SMART Domains

Protein: ENSMUSP00000089648
Gene: ENSMUSG00000069439

Domain	Start	End	E-Value	Type
Pfam:Ribosomal_L29	2	58	2.3e-22	PFAM
low complexity region	90	103	N/A	INTRINSIC

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.4%
20x: 95.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5730596B20Rik	A	T	6: 52,156,108 (GRCm39)		probably benign	Het
Aldh1l2	T	C	10: 83,338,336 (GRCm39)	K528E	probably benign	Het
Als2	A	G	1: 59,254,653 (GRCm39)	C235R	probably damaging	Het
Armc6	C	A	8: 70,677,799 (GRCm39)	V177L	probably benign	Het
Asb7	A	T	7: 66,328,959 (GRCm39)	V27D	probably damaging	Het
Bard1	A	G	1: 71,127,390 (GRCm39)	V73A	possibly damaging	Het
Ccdc150	A	G	1: 54,328,001 (GRCm39)	N361S	possibly damaging	Het
Cntn5	A	G	9: 10,419,076 (GRCm39)	L7P	probably benign	Het
Cst3	A	G	2: 148,713,951 (GRCm39)	S118P	probably damaging	Het
Cyp2a22	T	C	7: 26,638,254 (GRCm39)	D84G	probably damaging	Het
Ddo	A	G	10: 40,507,738 (GRCm39)	N45S	probably benign	Het
Drd4	T	C	7: 140,874,392 (GRCm39)	V319A	probably damaging	Het
Fat3	C	T	9: 15,871,792 (GRCm39)	R3533H	probably benign	Het
Fras1	A	T	5: 96,912,802 (GRCm39)	I3369F	probably damaging	Het
Gm5591	T	C	7: 38,220,058 (GRCm39)	S272G	probably benign	Het
Gm9776	A	G	13: 94,495,158 (GRCm39)		probably benign	Het
Kdm1b	C	T	13: 47,216,553 (GRCm39)	R308W	probably damaging	Het
Larp1b	A	T	3: 40,918,535 (GRCm39)	I59F	probably benign	Het
Ltn1	C	T	16: 87,200,961 (GRCm39)	A1092T	probably benign	Het
Map1b	A	G	13: 99,569,250 (GRCm39)	V1157A	unknown	Het
Mboat1	A	T	13: 30,379,724 (GRCm39)	M92L	probably benign	Het
Meis3	G	T	7: 15,916,378 (GRCm39)	L284F	probably damaging	Het
Muc20	T	C	16: 32,599,403 (GRCm39)	F3317L	probably benign	Het
Muc5b	G	T	7: 141,417,778 (GRCm39)	V3575F	probably damaging	Het
Polr1e	C	T	4: 45,018,724 (GRCm39)	T18I	possibly damaging	Het
Ppp4r1	G	T	17: 66,143,074 (GRCm39)	A764S	probably damaging	Het
Psg29	A	G	7: 16,942,727 (GRCm39)	T243A	probably benign	Het
Ptprn2	T	C	12: 116,685,753 (GRCm39)	Y71H	probably damaging	Het
Radx	T	A	X: 138,412,306 (GRCm39)	V439E	possibly damaging	Het
Rufy4	T	C	1: 74,186,822 (GRCm39)	C537R	probably damaging	Het
Sh3bp1	C	T	15: 78,795,622 (GRCm39)	P584S	probably benign	Het
Slc4a4	T	C	5: 89,373,807 (GRCm39)	V971A	probably damaging	Het
Stil	T	A	4: 114,862,163 (GRCm39)	H35Q	probably damaging	Het
Strn	A	T	17: 78,990,321 (GRCm39)	V65D	probably damaging	Het
Sumf1	T	C	6: 108,130,165 (GRCm39)	N185D	probably benign	Het
Timm29	T	C	9: 21,504,887 (GRCm39)	M185T	probably damaging	Het
Tjp3	T	C	10: 81,116,341 (GRCm39)	K251R	probably benign	Het
Tubgcp6	A	T	15: 89,006,806 (GRCm39)	M72K	probably damaging	Het
Utp14b	T	A	1: 78,642,442 (GRCm39)	D113E	possibly damaging	Het
Vps13b	A	G	15: 35,646,507 (GRCm39)	E1537G	probably damaging	Het
Zfr	A	G	15: 12,154,593 (GRCm39)	N592S	probably damaging	Het

Other mutations in Gm8444

Allele	Source	Chr	Coord	Type	Predicted Effect
R1167:Gm8444	UTSW	15	81,727,581 (GRCm39)	unclassified	probably benign
R2006:Gm8444	UTSW	15	81,727,775 (GRCm39)	unclassified	probably benign
R4274:Gm8444	UTSW	15	81,727,734 (GRCm39)	unclassified	probably benign
R5332:Gm8444	UTSW	15	81,727,902 (GRCm39)	unclassified	probably benign
R5807:Gm8444	UTSW	15	81,727,654 (GRCm39)	unclassified	probably benign
R6036:Gm8444	UTSW	15	81,727,794 (GRCm39)	unclassified	probably benign
R6036:Gm8444	UTSW	15	81,727,794 (GRCm39)	unclassified	probably benign
R6187:Gm8444	UTSW	15	81,727,807 (GRCm39)	unclassified	probably benign

Predicted Primers

PCR Primer
(F):5'- CAGGTCTAGGGGCTTGCA -3'
(R):5'- CTGGCCGCTAGAGATAATTACA -3'

Sequencing Primer
(F):5'- GCATTTCTTGCCCTTGAAGAATTTC -3'
(R):5'- AGCGGAATCTAATGCCTCTG -3'

Posted On

2015-02-05