Mutagenetix > Incidental Mutation

Incidental Mutation 'R3076:Mrgpre'

265211

Institutional Source

Beutler Lab

Gene Symbol

Mrgpre

Ensembl Gene

ENSMUSG00000048965

Gene Name

MAS-related GPR, member E

Synonyms

MrgE, C130069N09Rik

MMRRC Submission

040566-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R3076 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

143332100-143338237 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 143335033 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Threonine at position 157 (A157T)

Ref Sequence

ENSEMBL: ENSMUSP00000058000 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000054048]

AlphaFold

Q91ZB7

Predicted Effect

probably benign
Transcript: ENSMUST00000054048
AA Change: A157T

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000058000
Gene: ENSMUSG00000048965
AA Change: A157T

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	29	200	7.3e-7	PFAM
transmembrane domain	210	232	N/A	INTRINSIC
transmembrane domain	247	269	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000103756

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.4%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit alterations in the development but not maintenance of allodynia. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrd1	A	T	5: 129,206,169 (GRCm39)	I248F	probably benign	Het
Amigo2	C	T	15: 97,143,315 (GRCm39)	R369Q	probably damaging	Het
Aopep	A	C	13: 63,387,929 (GRCm39)	D3A	probably damaging	Het
Arfgef3	T	C	10: 18,479,278 (GRCm39)	I1446V	probably damaging	Het
Ascc2	T	A	11: 4,622,446 (GRCm39)	L504Q	probably damaging	Het
Atp1a3	A	G	7: 24,679,498 (GRCm39)	V932A	possibly damaging	Het
Cabcoco1	T	G	10: 68,361,475 (GRCm39)	Y8S	possibly damaging	Het
Cct8	A	G	16: 87,285,765 (GRCm39)	V231A	possibly damaging	Het
Col18a1	T	C	10: 76,924,762 (GRCm39)	K9R	possibly damaging	Het
Dlgap3	A	G	4: 127,089,499 (GRCm39)	Y365C	probably damaging	Het
Dock3	T	C	9: 106,818,725 (GRCm39)		probably null	Het
Gtf3c4	A	T	2: 28,725,165 (GRCm39)	V189E	possibly damaging	Het
Khdc3	C	A	9: 73,010,212 (GRCm39)	F89L	probably damaging	Het
Kif14	T	G	1: 136,447,383 (GRCm39)	I1396S	possibly damaging	Het
Magi1	T	C	6: 93,734,668 (GRCm39)	Q393R	possibly damaging	Het
Med28	A	G	5: 45,679,820 (GRCm39)	T68A	possibly damaging	Het
Meis1	A	T	11: 18,961,254 (GRCm39)	N206K	probably benign	Het
Mnt	G	C	11: 74,733,936 (GRCm39)		probably benign	Het
Mtpn	C	T	6: 35,498,879 (GRCm39)	V76I	possibly damaging	Het
Nbeal2	A	T	9: 110,460,768 (GRCm39)	W1702R	probably damaging	Het
Nhsl2	C	T	X: 101,121,201 (GRCm39)	R62W	probably damaging	Het
Npr2	T	A	4: 43,640,182 (GRCm39)	Y306N	probably damaging	Het
Nrsn1	T	C	13: 25,437,542 (GRCm39)	T129A	probably benign	Het
Nrxn3	T	G	12: 89,227,186 (GRCm39)	C274G	probably damaging	Het
Nyap2	T	C	1: 81,219,686 (GRCm39)		probably null	Het
Or1e29	G	A	11: 73,667,466 (GRCm39)	P229L	possibly damaging	Het
Or1o11	T	C	17: 37,756,375 (GRCm39)		probably benign	Het
Phldb2	T	C	16: 45,645,373 (GRCm39)	T403A	probably benign	Het
Plaa	T	C	4: 94,458,042 (GRCm39)	I643V	probably benign	Het
Ptprb	T	C	10: 116,179,931 (GRCm39)	S1450P	probably damaging	Het
Sh2d2a	T	C	3: 87,759,477 (GRCm39)	I296T	probably benign	Het
Shtn1	T	C	19: 58,983,518 (GRCm39)	E471G	probably damaging	Het
St7	T	G	6: 17,846,237 (GRCm39)	Y163*	probably null	Het
Svil	T	C	18: 5,116,055 (GRCm39)	S1623P	probably damaging	Het
Ugcg	T	G	4: 59,213,922 (GRCm39)	V168G	probably damaging	Het
Vmn1r223	G	A	13: 23,434,335 (GRCm39)	A310T	probably benign	Het
Zfp647	A	T	15: 76,802,209 (GRCm39)	M1K	probably null	Het

Other mutations in Mrgpre

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0671:Mrgpre	UTSW	7	143,335,254 (GRCm39)	missense	probably benign
R0732:Mrgpre	UTSW	7	143,335,303 (GRCm39)	missense	possibly damaging	0.81
R4572:Mrgpre	UTSW	7	143,334,841 (GRCm39)	missense	probably damaging	1.00
R4960:Mrgpre	UTSW	7	143,335,088 (GRCm39)	nonsense	probably null
R5341:Mrgpre	UTSW	7	143,335,246 (GRCm39)	missense	probably benign
R5352:Mrgpre	UTSW	7	143,334,831 (GRCm39)	missense	probably damaging	1.00
R5920:Mrgpre	UTSW	7	143,335,465 (GRCm39)	missense	probably benign	0.00
R6178:Mrgpre	UTSW	7	143,334,708 (GRCm39)	missense	possibly damaging	0.93
R6248:Mrgpre	UTSW	7	143,334,603 (GRCm39)	missense	probably benign	0.17
R6717:Mrgpre	UTSW	7	143,335,260 (GRCm39)	missense	probably damaging	1.00
R6962:Mrgpre	UTSW	7	143,334,799 (GRCm39)	missense	probably damaging	0.99
R7381:Mrgpre	UTSW	7	143,335,150 (GRCm39)	missense	probably damaging	1.00
R7574:Mrgpre	UTSW	7	143,335,087 (GRCm39)	missense	probably damaging	0.99
R8942:Mrgpre	UTSW	7	143,335,002 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- TTGGACAGCCAGAAGATGC -3'
(R):5'- TCTGCTACATTGTGGGCCTG -3'

Sequencing Primer
(F):5'- CAGCCAGAAGATGCCAAAGG -3'
(R):5'- TGACCACCTGTGTGTGT -3'

Posted On

2015-02-05