Mutagenetix > Incidental Mutation

Incidental Mutation 'R3076:Vmn1r223'

265230

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r223

Ensembl Gene

ENSMUSG00000069280

Gene Name

vomeronasal 1 receptor 223

Synonyms

Gm11330

MMRRC Submission

040566-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.053) question?

Stock #

R3076 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

23433408-23434493 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 23434335 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Threonine at position 310 (A310T)

Ref Sequence

ENSEMBL: ENSMUSP00000089312 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000091719]

AlphaFold

Q5SSA0

Predicted Effect

probably benign
Transcript: ENSMUST00000091719
AA Change: A310T

PolyPhen 2 Score 0.152 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000089312
Gene: ENSMUSG00000069280
AA Change: A310T

Domain	Start	End	E-Value	Type
low complexity region	46	59	N/A	INTRINSIC
Pfam:TAS2R	63	355	6.6e-10	PFAM
Pfam:V1R	94	357	2.1e-39	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrd1	A	T	5: 129,206,169 (GRCm39)	I248F	probably benign	Het
Amigo2	C	T	15: 97,143,315 (GRCm39)	R369Q	probably damaging	Het
Aopep	A	C	13: 63,387,929 (GRCm39)	D3A	probably damaging	Het
Arfgef3	T	C	10: 18,479,278 (GRCm39)	I1446V	probably damaging	Het
Ascc2	T	A	11: 4,622,446 (GRCm39)	L504Q	probably damaging	Het
Atp1a3	A	G	7: 24,679,498 (GRCm39)	V932A	possibly damaging	Het
Cabcoco1	T	G	10: 68,361,475 (GRCm39)	Y8S	possibly damaging	Het
Cct8	A	G	16: 87,285,765 (GRCm39)	V231A	possibly damaging	Het
Col18a1	T	C	10: 76,924,762 (GRCm39)	K9R	possibly damaging	Het
Dlgap3	A	G	4: 127,089,499 (GRCm39)	Y365C	probably damaging	Het
Dock3	T	C	9: 106,818,725 (GRCm39)		probably null	Het
Gtf3c4	A	T	2: 28,725,165 (GRCm39)	V189E	possibly damaging	Het
Khdc3	C	A	9: 73,010,212 (GRCm39)	F89L	probably damaging	Het
Kif14	T	G	1: 136,447,383 (GRCm39)	I1396S	possibly damaging	Het
Magi1	T	C	6: 93,734,668 (GRCm39)	Q393R	possibly damaging	Het
Med28	A	G	5: 45,679,820 (GRCm39)	T68A	possibly damaging	Het
Meis1	A	T	11: 18,961,254 (GRCm39)	N206K	probably benign	Het
Mnt	G	C	11: 74,733,936 (GRCm39)		probably benign	Het
Mrgpre	C	T	7: 143,335,033 (GRCm39)	A157T	probably benign	Het
Mtpn	C	T	6: 35,498,879 (GRCm39)	V76I	possibly damaging	Het
Nbeal2	A	T	9: 110,460,768 (GRCm39)	W1702R	probably damaging	Het
Nhsl2	C	T	X: 101,121,201 (GRCm39)	R62W	probably damaging	Het
Npr2	T	A	4: 43,640,182 (GRCm39)	Y306N	probably damaging	Het
Nrsn1	T	C	13: 25,437,542 (GRCm39)	T129A	probably benign	Het
Nrxn3	T	G	12: 89,227,186 (GRCm39)	C274G	probably damaging	Het
Nyap2	T	C	1: 81,219,686 (GRCm39)		probably null	Het
Or1e29	G	A	11: 73,667,466 (GRCm39)	P229L	possibly damaging	Het
Or1o11	T	C	17: 37,756,375 (GRCm39)		probably benign	Het
Phldb2	T	C	16: 45,645,373 (GRCm39)	T403A	probably benign	Het
Plaa	T	C	4: 94,458,042 (GRCm39)	I643V	probably benign	Het
Ptprb	T	C	10: 116,179,931 (GRCm39)	S1450P	probably damaging	Het
Sh2d2a	T	C	3: 87,759,477 (GRCm39)	I296T	probably benign	Het
Shtn1	T	C	19: 58,983,518 (GRCm39)	E471G	probably damaging	Het
St7	T	G	6: 17,846,237 (GRCm39)	Y163*	probably null	Het
Svil	T	C	18: 5,116,055 (GRCm39)	S1623P	probably damaging	Het
Ugcg	T	G	4: 59,213,922 (GRCm39)	V168G	probably damaging	Het
Zfp647	A	T	15: 76,802,209 (GRCm39)	M1K	probably null	Het

Other mutations in Vmn1r223

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00661:Vmn1r223	APN	13	23,434,254 (GRCm39)	missense	probably damaging	1.00
IGL01016:Vmn1r223	APN	13	23,434,237 (GRCm39)	missense	probably damaging	1.00
IGL01353:Vmn1r223	APN	13	23,433,426 (GRCm39)	missense	unknown
IGL01941:Vmn1r223	APN	13	23,434,407 (GRCm39)	missense	possibly damaging	0.94
IGL02976:Vmn1r223	APN	13	23,434,165 (GRCm39)	missense	probably damaging	1.00
IGL03064:Vmn1r223	APN	13	23,434,153 (GRCm39)	missense	probably damaging	1.00
IGL03136:Vmn1r223	APN	13	23,433,933 (GRCm39)	missense	possibly damaging	0.81
PIT4812001:Vmn1r223	UTSW	13	23,434,060 (GRCm39)	missense	probably damaging	0.99
R1468:Vmn1r223	UTSW	13	23,434,038 (GRCm39)	missense	possibly damaging	0.81
R1468:Vmn1r223	UTSW	13	23,434,038 (GRCm39)	missense	possibly damaging	0.81
R1592:Vmn1r223	UTSW	13	23,433,837 (GRCm39)	missense	possibly damaging	0.57
R1640:Vmn1r223	UTSW	13	23,434,348 (GRCm39)	missense	probably damaging	1.00
R2116:Vmn1r223	UTSW	13	23,433,832 (GRCm39)	missense	probably damaging	1.00
R4229:Vmn1r223	UTSW	13	23,433,585 (GRCm39)	missense	probably benign	0.08
R4230:Vmn1r223	UTSW	13	23,433,585 (GRCm39)	missense	probably benign	0.08
R5420:Vmn1r223	UTSW	13	23,433,675 (GRCm39)	missense	probably benign	0.02
R6412:Vmn1r223	UTSW	13	23,433,825 (GRCm39)	missense	probably benign	0.05
R6424:Vmn1r223	UTSW	13	23,434,345 (GRCm39)	missense	probably damaging	1.00
R6607:Vmn1r223	UTSW	13	23,433,919 (GRCm39)	missense	probably damaging	1.00
R6944:Vmn1r223	UTSW	13	23,433,483 (GRCm39)	missense	unknown
R7256:Vmn1r223	UTSW	13	23,434,036 (GRCm39)	missense	probably damaging	1.00
R8241:Vmn1r223	UTSW	13	23,433,982 (GRCm39)	missense	probably benign	0.38
R8315:Vmn1r223	UTSW	13	23,434,339 (GRCm39)	missense	probably damaging	1.00
R8347:Vmn1r223	UTSW	13	23,434,020 (GRCm39)	missense	probably damaging	1.00
R8975:Vmn1r223	UTSW	13	23,434,248 (GRCm39)	missense	possibly damaging	0.84
R9075:Vmn1r223	UTSW	13	23,433,600 (GRCm39)	missense	possibly damaging	0.50

Predicted Primers

PCR Primer
(F):5'- TCCAGGCACACAGTTAAGTGG -3'
(R):5'- GAATTTCTTATTTCTCAGGGGACAC -3'

Sequencing Primer
(F):5'- GCACACAGTTAAGTGGCTGTTCC -3'
(R):5'- CTTATTTCTCAGGGGACACAGCAG -3'

Posted On

2015-02-05