Mutagenetix > Incidental Mutation

Incidental Mutation 'R3078:Vmn2r2'

265279

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r2

Ensembl Gene

ENSMUSG00000043897

Gene Name

vomeronasal 2, receptor 2

Synonyms

MMRRC Submission

040568-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.108) question?

Stock #

R3078 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

64022699-64049349 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 64042053 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 221 (I221V)

Ref Sequence

ENSEMBL: ENSMUSP00000135110 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077958] [ENSMUST00000177151]

AlphaFold

L7N2E9

Predicted Effect

probably benign
Transcript: ENSMUST00000077958
AA Change: I137V

PolyPhen 2 Score 0.129 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000077109
Gene: ENSMUSG00000043897
AA Change: I137V

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	416	4.5e-80	PFAM
Pfam:NCD3G	458	511	8.3e-18	PFAM
Pfam:7tm_3	542	779	1.3e-75	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000177151
AA Change: I221V

PolyPhen 2 Score 0.216 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000135110
Gene: ENSMUSG00000043897
AA Change: I221V

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	83	500	1.6e-84	PFAM
Pfam:NCD3G	542	595	2.6e-17	PFAM
Pfam:7tm_3	628	862	1.9e-47	PFAM

Meta Mutation Damage Score

0.1502

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.3%

Validation Efficiency

93% (42/45)

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca12	T	C	1: 71,306,764 (GRCm39)	I1981V	probably benign	Het
Actr3b	A	G	5: 26,027,440 (GRCm39)	Y37C	probably damaging	Het
Adgrd1	A	T	5: 129,206,169 (GRCm39)	I248F	probably benign	Het
Alg10b	T	C	15: 90,112,139 (GRCm39)	S328P	probably benign	Het
C5ar2	G	A	7: 15,971,349 (GRCm39)	R193C	probably damaging	Het
Cct8	A	G	16: 87,285,765 (GRCm39)	V231A	possibly damaging	Het
Clca4a	C	T	3: 144,674,014 (GRCm39)	M240I	probably damaging	Het
Cmya5	A	T	13: 93,185,435 (GRCm39)	I3520N	probably damaging	Het
Dock6	G	T	9: 21,757,050 (GRCm39)		probably benign	Het
Dynlrb1	T	A	2: 155,091,865 (GRCm39)	I99N	probably damaging	Het
Ebf4	G	A	2: 130,148,419 (GRCm39)	D77N	probably damaging	Het
Fbxw10	C	T	11: 62,758,339 (GRCm39)		probably benign	Het
Gm10801	T	C	2: 98,494,197 (GRCm39)	I113T	probably damaging	Het
Gm5499	T	C	17: 87,386,314 (GRCm39)		noncoding transcript	Het
Gm9913	A	G	2: 125,348,459 (GRCm39)		probably benign	Het
Herc2	A	G	7: 55,786,991 (GRCm39)	N1612S	probably benign	Het
Ifnar2	T	C	16: 91,182,889 (GRCm39)	S53P	possibly damaging	Het
Inpp5j	T	A	11: 3,453,124 (GRCm39)		probably null	Het
Insyn2a	A	G	7: 134,519,750 (GRCm39)	I260T	probably benign	Het
Iqca1l	G	C	5: 24,751,664 (GRCm39)	T528S	probably benign	Het
Kif14	T	G	1: 136,447,383 (GRCm39)	I1396S	possibly damaging	Het
Med28	A	G	5: 45,679,820 (GRCm39)	T68A	possibly damaging	Het
Meis1	A	T	11: 18,961,254 (GRCm39)	N206K	probably benign	Het
Mfsd14a	T	C	3: 116,441,566 (GRCm39)		probably benign	Het
Mnt	G	C	11: 74,733,936 (GRCm39)		probably benign	Het
Mto1	A	G	9: 78,365,310 (GRCm39)	Y413C	probably damaging	Het
Myo7b	T	C	18: 32,100,237 (GRCm39)	D1599G	probably benign	Het
Myoz1	T	C	14: 20,703,685 (GRCm39)		probably benign	Het
Nhsl2	C	T	X: 101,121,201 (GRCm39)	R62W	probably damaging	Het
Npr2	T	A	4: 43,640,182 (GRCm39)	Y306N	probably damaging	Het
Nrxn3	T	G	12: 89,227,186 (GRCm39)	C274G	probably damaging	Het
Or1e29	G	A	11: 73,667,466 (GRCm39)	P229L	possibly damaging	Het
Or5ac22	A	T	16: 59,135,089 (GRCm39)	M227K	probably benign	Het
Phldb2	T	C	16: 45,645,373 (GRCm39)	T403A	probably benign	Het
Plaa	T	C	4: 94,458,042 (GRCm39)	I643V	probably benign	Het
Slc28a2b	T	C	2: 122,344,895 (GRCm39)	L167P	possibly damaging	Het
Stau1	T	C	2: 166,796,936 (GRCm39)	I154V	possibly damaging	Het
Ugcg	T	G	4: 59,213,922 (GRCm39)	V168G	probably damaging	Het
Wdr93	T	C	7: 79,402,241 (GRCm39)	I180T	possibly damaging	Het
Whamm	G	C	7: 81,221,532 (GRCm39)	G155R	probably damaging	Het
Wnt5a	T	A	14: 28,235,140 (GRCm39)	Y41*	probably null	Het

Other mutations in Vmn2r2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00326:Vmn2r2	APN	3	64,041,319 (GRCm39)	splice site	probably benign
IGL00980:Vmn2r2	APN	3	64,024,601 (GRCm39)	missense	probably benign	0.11
IGL01389:Vmn2r2	APN	3	64,024,430 (GRCm39)	missense	probably damaging	0.97
IGL01804:Vmn2r2	APN	3	64,041,677 (GRCm39)	missense	possibly damaging	0.91
IGL02750:Vmn2r2	APN	3	64,024,823 (GRCm39)	missense	probably damaging	1.00
IGL02829:Vmn2r2	APN	3	64,026,172 (GRCm39)	splice site	probably benign
IGL03036:Vmn2r2	APN	3	64,024,321 (GRCm39)	missense	probably benign	0.01
IGL03174:Vmn2r2	APN	3	64,024,544 (GRCm39)	nonsense	probably null
PIT4151001:Vmn2r2	UTSW	3	64,024,334 (GRCm39)	missense	possibly damaging	0.67
R0029:Vmn2r2	UTSW	3	64,024,365 (GRCm39)	missense	probably benign	0.00
R0310:Vmn2r2	UTSW	3	64,042,039 (GRCm39)	missense	probably damaging	1.00
R0357:Vmn2r2	UTSW	3	64,041,320 (GRCm39)	splice site	probably null
R0637:Vmn2r2	UTSW	3	64,033,999 (GRCm39)	missense	probably benign	0.00
R1626:Vmn2r2	UTSW	3	64,041,921 (GRCm39)	missense	possibly damaging	0.61
R1662:Vmn2r2	UTSW	3	64,024,551 (GRCm39)	missense	probably benign	0.00
R1710:Vmn2r2	UTSW	3	64,024,820 (GRCm39)	missense	probably benign	0.19
R1797:Vmn2r2	UTSW	3	64,042,128 (GRCm39)	missense	probably benign	0.00
R1862:Vmn2r2	UTSW	3	64,041,942 (GRCm39)	missense	possibly damaging	0.79
R1971:Vmn2r2	UTSW	3	64,034,121 (GRCm39)	missense	probably damaging	1.00
R2051:Vmn2r2	UTSW	3	64,024,766 (GRCm39)	missense	possibly damaging	0.77
R2099:Vmn2r2	UTSW	3	64,024,474 (GRCm39)	missense	probably damaging	1.00
R2275:Vmn2r2	UTSW	3	64,023,930 (GRCm39)	missense	probably benign	0.00
R3418:Vmn2r2	UTSW	3	64,024,320 (GRCm39)	missense	probably benign	0.00
R3419:Vmn2r2	UTSW	3	64,024,320 (GRCm39)	missense	probably benign	0.00
R3959:Vmn2r2	UTSW	3	64,047,947 (GRCm39)	missense	probably benign
R4230:Vmn2r2	UTSW	3	64,041,912 (GRCm39)	missense	probably benign	0.00
R4258:Vmn2r2	UTSW	3	64,042,118 (GRCm39)	missense	probably damaging	1.00
R4810:Vmn2r2	UTSW	3	64,044,883 (GRCm39)	missense	probably damaging	1.00
R4822:Vmn2r2	UTSW	3	64,041,960 (GRCm39)	missense	probably damaging	0.99
R4919:Vmn2r2	UTSW	3	64,024,578 (GRCm39)	missense	possibly damaging	0.56
R4925:Vmn2r2	UTSW	3	64,044,892 (GRCm39)	start codon destroyed	probably null	0.03
R4954:Vmn2r2	UTSW	3	64,047,905 (GRCm39)	missense	probably benign	0.00
R5071:Vmn2r2	UTSW	3	64,024,321 (GRCm39)	missense	probably benign	0.01
R5315:Vmn2r2	UTSW	3	64,024,377 (GRCm39)	missense	probably benign	0.37
R5450:Vmn2r2	UTSW	3	64,034,011 (GRCm39)	missense	probably benign
R5577:Vmn2r2	UTSW	3	64,024,416 (GRCm39)	missense	probably benign	0.00
R5595:Vmn2r2	UTSW	3	64,034,036 (GRCm39)	missense	possibly damaging	0.74
R5727:Vmn2r2	UTSW	3	64,024,608 (GRCm39)	missense	probably benign	0.41
R5810:Vmn2r2	UTSW	3	64,024,815 (GRCm39)	missense	probably damaging	1.00
R5919:Vmn2r2	UTSW	3	64,044,723 (GRCm39)	missense	probably benign	0.39
R6052:Vmn2r2	UTSW	3	64,024,782 (GRCm39)	missense	possibly damaging	0.56
R6084:Vmn2r2	UTSW	3	64,024,467 (GRCm39)	missense	probably benign	0.01
R6299:Vmn2r2	UTSW	3	64,024,074 (GRCm39)	nonsense	probably null
R6762:Vmn2r2	UTSW	3	64,041,870 (GRCm39)	missense	probably damaging	1.00
R6858:Vmn2r2	UTSW	3	64,044,915 (GRCm39)	missense	probably damaging	1.00
R6889:Vmn2r2	UTSW	3	64,024,688 (GRCm39)	missense	probably damaging	0.99
R6990:Vmn2r2	UTSW	3	64,024,608 (GRCm39)	missense	probably benign	0.02
R7195:Vmn2r2	UTSW	3	64,023,900 (GRCm39)	missense	probably benign	0.01
R7269:Vmn2r2	UTSW	3	64,033,998 (GRCm39)	missense	probably benign	0.32
R7699:Vmn2r2	UTSW	3	64,024,536 (GRCm39)	missense	possibly damaging	0.69
R7717:Vmn2r2	UTSW	3	64,042,019 (GRCm39)	missense	possibly damaging	0.95
R7798:Vmn2r2	UTSW	3	64,041,518 (GRCm39)	missense	possibly damaging	0.60
R7914:Vmn2r2	UTSW	3	64,041,526 (GRCm39)	missense	probably benign	0.20
R7974:Vmn2r2	UTSW	3	64,024,808 (GRCm39)	missense	probably damaging	0.99
R8394:Vmn2r2	UTSW	3	64,044,858 (GRCm39)	missense	probably damaging	1.00
R8478:Vmn2r2	UTSW	3	64,024,257 (GRCm39)	missense	possibly damaging	0.87
R8731:Vmn2r2	UTSW	3	64,024,404 (GRCm39)	missense	probably benign	0.16
R9035:Vmn2r2	UTSW	3	64,024,172 (GRCm39)	missense	probably damaging	1.00
R9182:Vmn2r2	UTSW	3	64,044,802 (GRCm39)	missense	probably damaging	1.00
R9225:Vmn2r2	UTSW	3	64,034,021 (GRCm39)	missense	probably benign	0.00
R9266:Vmn2r2	UTSW	3	64,024,057 (GRCm39)	missense	probably damaging	0.98
R9427:Vmn2r2	UTSW	3	64,041,945 (GRCm39)	missense	probably damaging	1.00
R9659:Vmn2r2	UTSW	3	64,041,942 (GRCm39)	missense	possibly damaging	0.79
R9666:Vmn2r2	UTSW	3	64,023,870 (GRCm39)	missense	probably benign	0.08
R9771:Vmn2r2	UTSW	3	64,042,079 (GRCm39)	missense	possibly damaging	0.88
R9788:Vmn2r2	UTSW	3	64,041,942 (GRCm39)	missense	possibly damaging	0.79
X0024:Vmn2r2	UTSW	3	64,044,707 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- CACTTCCAGCACAAATGGGC -3'
(R):5'- AAGTTGGTTCCTGTTTCCTAGTAAC -3'

Sequencing Primer
(F):5'- ATGGGCCGAGGTCAATGTC -3'
(R):5'- GGGCTATACTTCTTCCTGTG -3'

Posted On

2015-02-05