Mutagenetix > Incidental Mutation

Incidental Mutation 'R3084:Vmn2r85'

265540

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r85

Ensembl Gene

ENSMUSG00000092048

Gene Name

vomeronasal 2, receptor 85

Synonyms

EG623734

MMRRC Submission

040573-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R3084 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

130253658-130266615 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 130261081 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Valine at position 419 (M419V)

Ref Sequence

ENSEMBL: ENSMUSP00000128792 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171811]

AlphaFold

G3UW56

Predicted Effect

probably benign
Transcript: ENSMUST00000171811
AA Change: M419V

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000128792
Gene: ENSMUSG00000092048
AA Change: M419V

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	77	425	9e-26	PFAM
Pfam:NCD3G	508	562	1.1e-18	PFAM
Pfam:7tm_3	595	831	3.7e-54	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.5%

Validation Efficiency

100% (43/43)

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgra3	A	T	5: 50,170,733 (GRCm39)		probably null	Het
Alms1	A	G	6: 85,655,122 (GRCm39)	R3223G	probably benign	Het
Arhgef26	T	C	3: 62,285,037 (GRCm39)	V431A	probably benign	Het
Cabyr	T	C	18: 12,884,023 (GRCm39)	V170A	probably damaging	Het
Cenpe	A	G	3: 134,946,782 (GRCm39)	E1099G	probably damaging	Het
Creb3l1	G	T	2: 91,825,789 (GRCm39)		probably null	Het
Cyp2c38	T	C	19: 39,390,145 (GRCm39)	I352V	probably benign	Het
Dhrs2	G	T	14: 55,477,301 (GRCm39)	V179L	probably benign	Het
Dhx9	T	C	1: 153,341,445 (GRCm39)	D601G	probably benign	Het
Dlg5	T	C	14: 24,216,258 (GRCm39)	T595A	probably damaging	Het
Fhip1a	T	A	3: 85,573,275 (GRCm39)		probably null	Het
Frk	G	A	10: 34,483,950 (GRCm39)	G437D	probably damaging	Het
Gm10608	CAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA	CAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA	9: 118,989,784 (GRCm39)		probably null	Het
Gm5699	G	T	1: 31,037,873 (GRCm39)		noncoding transcript	Het
Gsg1l	C	T	7: 125,490,852 (GRCm39)	R284H	probably benign	Het
Ifit3	A	T	19: 34,564,640 (GRCm39)	H62L	probably damaging	Het
Krt34	T	C	11: 99,931,847 (GRCm39)	N124S	probably damaging	Het
Megf8	T	A	7: 25,048,444 (GRCm39)	Y1706N	probably damaging	Het
Mmd	C	T	11: 90,156,911 (GRCm39)	R129C	probably damaging	Het
Or4f60	C	A	2: 111,902,320 (GRCm39)	G203*	probably null	Het
Or4k36	A	G	2: 111,146,461 (GRCm39)	I212M	probably benign	Het
Pde6d	T	C	1: 86,475,248 (GRCm39)		probably null	Het
Pomt1	G	T	2: 32,134,252 (GRCm39)	V258L	probably benign	Het
Ppp2r5e	T	C	12: 75,515,390 (GRCm39)	I215V	probably benign	Het
Ranbp10	A	T	8: 106,501,263 (GRCm39)	L329Q	probably damaging	Het
Robo1	T	A	16: 72,801,625 (GRCm39)	L1083Q	probably benign	Het
Rsph4a	G	C	10: 33,785,198 (GRCm39)	V370L	probably damaging	Het
Smarcc2	T	C	10: 128,324,028 (GRCm39)		probably benign	Het
Sun1	T	C	5: 139,221,356 (GRCm39)	V357A	probably benign	Het
Svop	T	C	5: 114,180,299 (GRCm39)	T283A	probably benign	Het
Tep1	A	T	14: 51,064,511 (GRCm39)		probably null	Het
Tet1	T	C	10: 62,715,400 (GRCm39)	K132E	probably benign	Het
Tex15	A	T	8: 34,064,913 (GRCm39)	N1448Y	probably benign	Het
Tktl2	T	A	8: 66,965,858 (GRCm39)	M472K	possibly damaging	Het
Tnrc6b	T	G	15: 80,764,448 (GRCm39)	L650W	probably damaging	Het
Ttf2	C	T	3: 100,855,580 (GRCm39)	G872R	possibly damaging	Het
Vmn1r17	A	G	6: 57,337,768 (GRCm39)	V199A	probably damaging	Het
Vmn1r178	A	G	7: 23,593,331 (GRCm39)	I126M	possibly damaging	Het
Washc2	A	G	6: 116,204,454 (GRCm39)	N454S	probably benign	Het
Xirp2	T	G	2: 67,339,393 (GRCm39)	F545V	probably damaging	Het
Zfyve26	G	T	12: 79,312,457 (GRCm39)		probably benign	Het

Other mutations in Vmn2r85

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01160:Vmn2r85	APN	10	130,254,690 (GRCm39)	missense	probably benign	0.22
IGL01298:Vmn2r85	APN	10	130,254,690 (GRCm39)	missense	probably benign	0.22
IGL01361:Vmn2r85	APN	10	130,254,690 (GRCm39)	missense	probably benign	0.22
IGL02185:Vmn2r85	APN	10	130,254,561 (GRCm39)	missense	probably benign	0.13
IGL02505:Vmn2r85	APN	10	130,261,449 (GRCm39)	missense	probably damaging	1.00
IGL02607:Vmn2r85	APN	10	130,262,290 (GRCm39)	missense	possibly damaging	0.89
IGL02755:Vmn2r85	APN	10	130,261,381 (GRCm39)	missense	probably damaging	0.98
IGL03188:Vmn2r85	APN	10	130,254,612 (GRCm39)	missense	probably benign	0.16
IGL03366:Vmn2r85	APN	10	130,262,328 (GRCm39)	missense	probably benign	0.00
IGL03397:Vmn2r85	APN	10	130,261,263 (GRCm39)	missense	probably damaging	1.00
PIT4445001:Vmn2r85	UTSW	10	130,261,572 (GRCm39)	missense	probably benign	0.00
R0066:Vmn2r85	UTSW	10	130,261,770 (GRCm39)	missense	probably damaging	1.00
R0128:Vmn2r85	UTSW	10	130,255,054 (GRCm39)	splice site	probably benign
R0130:Vmn2r85	UTSW	10	130,255,054 (GRCm39)	splice site	probably benign
R0503:Vmn2r85	UTSW	10	130,258,609 (GRCm39)	missense	probably damaging	1.00
R0827:Vmn2r85	UTSW	10	130,265,387 (GRCm39)	missense	possibly damaging	0.89
R1432:Vmn2r85	UTSW	10	130,261,155 (GRCm39)	missense	possibly damaging	0.74
R1521:Vmn2r85	UTSW	10	130,261,788 (GRCm39)	missense	probably damaging	0.99
R2029:Vmn2r85	UTSW	10	130,261,443 (GRCm39)	nonsense	probably null
R2034:Vmn2r85	UTSW	10	130,262,242 (GRCm39)	splice site	probably benign
R2852:Vmn2r85	UTSW	10	130,255,035 (GRCm39)	missense	probably benign	0.03
R2853:Vmn2r85	UTSW	10	130,255,035 (GRCm39)	missense	probably benign	0.03
R3085:Vmn2r85	UTSW	10	130,261,081 (GRCm39)	missense	probably benign	0.00
R3430:Vmn2r85	UTSW	10	130,254,758 (GRCm39)	missense	probably damaging	0.97
R3694:Vmn2r85	UTSW	10	130,254,171 (GRCm39)	missense	probably damaging	0.99
R3932:Vmn2r85	UTSW	10	130,254,336 (GRCm39)	missense	probably damaging	1.00
R4207:Vmn2r85	UTSW	10	130,254,574 (GRCm39)	missense	probably damaging	1.00
R4628:Vmn2r85	UTSW	10	130,261,235 (GRCm39)	missense	probably benign	0.00
R4814:Vmn2r85	UTSW	10	130,254,567 (GRCm39)	missense	probably benign	0.12
R4948:Vmn2r85	UTSW	10	130,254,990 (GRCm39)	missense	probably damaging	1.00
R4951:Vmn2r85	UTSW	10	130,261,113 (GRCm39)	missense	probably damaging	1.00
R4959:Vmn2r85	UTSW	10	130,257,302 (GRCm39)	missense	probably damaging	1.00
R5336:Vmn2r85	UTSW	10	130,258,574 (GRCm39)	missense	possibly damaging	0.63
R5643:Vmn2r85	UTSW	10	130,262,343 (GRCm39)	missense	probably damaging	1.00
R6061:Vmn2r85	UTSW	10	130,261,531 (GRCm39)	missense	probably benign	0.09
R6115:Vmn2r85	UTSW	10	130,258,672 (GRCm39)	missense	probably damaging	1.00
R6190:Vmn2r85	UTSW	10	130,261,330 (GRCm39)	missense	possibly damaging	0.88
R6518:Vmn2r85	UTSW	10	130,265,281 (GRCm39)	missense	probably benign	0.00
R6533:Vmn2r85	UTSW	10	130,262,529 (GRCm39)	missense	probably benign	0.00
R6610:Vmn2r85	UTSW	10	130,261,838 (GRCm39)	missense	probably damaging	0.97
R6809:Vmn2r85	UTSW	10	130,261,795 (GRCm39)	missense	probably benign
R6962:Vmn2r85	UTSW	10	130,261,452 (GRCm39)	missense	probably damaging	0.99
R7075:Vmn2r85	UTSW	10	130,258,557 (GRCm39)	missense	probably benign	0.06
R7104:Vmn2r85	UTSW	10	130,262,376 (GRCm39)	missense	probably benign
R7424:Vmn2r85	UTSW	10	130,254,849 (GRCm39)	missense	probably damaging	1.00
R7516:Vmn2r85	UTSW	10	130,254,852 (GRCm39)	missense	probably damaging	1.00
R7537:Vmn2r85	UTSW	10	130,258,735 (GRCm39)	missense	probably benign	0.01
R7768:Vmn2r85	UTSW	10	130,254,562 (GRCm39)	missense	probably damaging	1.00
R7810:Vmn2r85	UTSW	10	130,261,081 (GRCm39)	missense	probably benign	0.00
R8078:Vmn2r85	UTSW	10	130,265,364 (GRCm39)	nonsense	probably null
R8115:Vmn2r85	UTSW	10	130,261,820 (GRCm39)	missense	probably benign	0.06
R8262:Vmn2r85	UTSW	10	130,254,738 (GRCm39)	missense	probably damaging	0.98
R8395:Vmn2r85	UTSW	10	130,261,797 (GRCm39)	missense	probably damaging	0.99
R8409:Vmn2r85	UTSW	10	130,261,257 (GRCm39)	missense	probably benign	0.16
R8547:Vmn2r85	UTSW	10	130,261,311 (GRCm39)	missense	probably damaging	1.00
R8875:Vmn2r85	UTSW	10	130,254,171 (GRCm39)	missense	probably damaging	0.99
R9035:Vmn2r85	UTSW	10	130,261,479 (GRCm39)	missense	probably benign
R9040:Vmn2r85	UTSW	10	130,254,311 (GRCm39)	missense	probably damaging	1.00
R9115:Vmn2r85	UTSW	10	130,254,153 (GRCm39)	missense	probably benign	0.00
R9182:Vmn2r85	UTSW	10	130,265,350 (GRCm39)	missense	probably benign	0.00
R9245:Vmn2r85	UTSW	10	130,261,534 (GRCm39)	missense	probably damaging	1.00
R9245:Vmn2r85	UTSW	10	130,255,033 (GRCm39)	missense	possibly damaging	0.92
R9405:Vmn2r85	UTSW	10	130,261,215 (GRCm39)	missense	probably damaging	0.99
R9502:Vmn2r85	UTSW	10	130,261,387 (GRCm39)	missense	probably damaging	0.99
R9520:Vmn2r85	UTSW	10	130,254,993 (GRCm39)	missense	probably benign
R9653:Vmn2r85	UTSW	10	130,261,694 (GRCm39)	missense	probably damaging	0.99
Z1176:Vmn2r85	UTSW	10	130,261,713 (GRCm39)	missense	probably damaging	0.99
Z1177:Vmn2r85	UTSW	10	130,254,776 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AAGATCATATGCCCCAGTACAGG -3'
(R):5'- TTCTGAGTCAATACTGAGGTGG -3'

Sequencing Primer
(F):5'- CCAGTACAGGGGAATGCC -3'
(R):5'- GAGTCAATACTGAGGTGGAATTATC -3'

Posted On

2015-02-05