Mutagenetix > Incidental Mutation

Incidental Mutation 'R3085:Or4f60'

265563

Institutional Source

Beutler Lab

Gene Symbol

Or4f60

Ensembl Gene

ENSMUSG00000074946

Gene Name

olfactory receptor family 4 subfamily F member 60

Synonyms

GA_x6K02T2Q125-73119859-73118924, MOR245-23, Olfr1313

MMRRC Submission

040574-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.054) question?

Stock #

R3085 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

111901991-111902928 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to A at 111902320 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Stop codon at position 203 (G203*)

Ref Sequence

ENSEMBL: ENSMUSP00000148886 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099599] [ENSMUST00000213577] [ENSMUST00000216071]

AlphaFold

Q7TQW8

Predicted Effect

probably null
Transcript: ENSMUST00000099599
AA Change: G203*

SMART Domains

Protein: ENSMUSP00000097194
Gene: ENSMUSG00000074946
AA Change: G203*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	304	2.2e-41	PFAM
Pfam:7tm_1	41	286	2.8e-24	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000213577
AA Change: G203*

Predicted Effect

probably null
Transcript: ENSMUST00000216071
AA Change: G203*

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.4%

Validation Efficiency

100% (63/63)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc12	T	A	8: 87,270,536 (GRCm39)		probably benign	Het
Adrm1	T	C	2: 179,816,094 (GRCm39)		probably null	Het
Aldh18a1	T	C	19: 40,562,813 (GRCm39)	I76V	probably benign	Het
Atp1b2	T	C	11: 69,493,705 (GRCm39)	K125E	possibly damaging	Het
Cabyr	T	C	18: 12,884,023 (GRCm39)	V170A	probably damaging	Het
Cdh8	T	C	8: 99,923,018 (GRCm39)	T293A	probably benign	Het
Cenpc1	A	T	5: 86,185,476 (GRCm39)	V345D	probably benign	Het
Col4a3	A	G	1: 82,628,979 (GRCm39)	E131G	unknown	Het
Col4a4	C	T	1: 82,507,285 (GRCm39)		probably null	Het
Creb3l1	G	T	2: 91,825,789 (GRCm39)		probably null	Het
Dhx9	T	C	1: 153,341,445 (GRCm39)	D601G	probably benign	Het
Dipk1a	G	T	5: 108,062,290 (GRCm39)	D28E	probably damaging	Het
Dnaaf4	T	A	9: 72,879,688 (GRCm39)	N289K	probably benign	Het
Eml6	T	C	11: 29,759,332 (GRCm39)	E807G	probably damaging	Het
Exog	C	A	9: 119,291,518 (GRCm39)	T241K	probably benign	Het
Eya1	T	C	1: 14,344,314 (GRCm39)	D109G	probably benign	Het
Fat2	C	T	11: 55,142,997 (GRCm39)	R4284H	possibly damaging	Het
Fbxw13	C	A	9: 109,013,299 (GRCm39)	G130*	probably null	Het
Fcrl5	A	G	3: 87,353,771 (GRCm39)	Y372C	probably damaging	Het
Gli3	T	C	13: 15,835,526 (GRCm39)	S435P	probably damaging	Het
Gsg1l	C	T	7: 125,490,852 (GRCm39)	R284H	probably benign	Het
Havcr1	C	T	11: 46,647,052 (GRCm39)	T162I	probably damaging	Het
Igdcc4	T	C	9: 65,039,340 (GRCm39)	F947S	probably damaging	Het
Irx1	G	T	13: 72,111,411 (GRCm39)	A66E	probably damaging	Het
Klf11	T	C	12: 24,705,490 (GRCm39)	S315P	probably benign	Het
Kynu	A	G	2: 43,492,312 (GRCm39)	M190V	probably benign	Het
Lrp2	T	C	2: 69,297,479 (GRCm39)	T3161A	probably benign	Het
Macrod1	G	T	19: 7,173,859 (GRCm39)	A208S	probably damaging	Het
Mamdc2	G	A	19: 23,288,296 (GRCm39)	H581Y	possibly damaging	Het
Megf8	T	A	7: 25,048,444 (GRCm39)	Y1706N	probably damaging	Het
Mthfd1l	G	A	10: 4,040,007 (GRCm39)	R806H	probably benign	Het
Nags	T	A	11: 102,036,810 (GRCm39)	V133D	probably damaging	Het
Nrg3	C	A	14: 38,092,906 (GRCm39)	D560Y	probably damaging	Het
Or4k36	A	G	2: 111,146,461 (GRCm39)	I212M	probably benign	Het
Or4p23	A	G	2: 88,576,488 (GRCm39)	F248S	probably damaging	Het
Or5af2	T	C	11: 58,708,553 (GRCm39)	S240P	probably damaging	Het
Orc4	G	A	2: 48,827,501 (GRCm39)	P31S	probably benign	Het
Pde6d	T	C	1: 86,475,248 (GRCm39)		probably null	Het
Plekhf1	T	C	7: 37,921,001 (GRCm39)	E189G	probably benign	Het
Ppm1b	A	T	17: 85,321,288 (GRCm39)	I477L	probably benign	Het
Rad51ap2	C	A	12: 11,506,758 (GRCm39)	Q227K	possibly damaging	Het
Rnf125	T	C	18: 21,110,787 (GRCm39)	V15A	probably benign	Het
Rnps1	C	T	17: 24,631,393 (GRCm39)		probably benign	Het
Robo1	A	G	16: 72,798,898 (GRCm39)	I953V	possibly damaging	Het
Sash1	A	T	10: 8,618,186 (GRCm39)		probably null	Het
Smarcc2	T	C	10: 128,324,028 (GRCm39)		probably benign	Het
Stx12	T	A	4: 132,584,672 (GRCm39)	E224V	probably damaging	Het
Sun1	T	C	5: 139,221,356 (GRCm39)	V357A	probably benign	Het
Synpo2l	T	C	14: 20,712,248 (GRCm39)	D350G	probably damaging	Het
Tex15	A	T	8: 34,064,913 (GRCm39)	N1448Y	probably benign	Het
Tfpi	A	G	2: 84,273,227 (GRCm39)		probably benign	Het
Tktl2	T	A	8: 66,965,858 (GRCm39)	M472K	possibly damaging	Het
Tmem40	T	C	6: 115,718,576 (GRCm39)	D43G	possibly damaging	Het
Tshz2	T	A	2: 169,725,871 (GRCm39)	C156S	probably benign	Het
Vmn2r101	T	A	17: 19,809,077 (GRCm39)		probably null	Het
Vmn2r85	T	C	10: 130,261,081 (GRCm39)	M419V	probably benign	Het
Wdr73	G	A	7: 80,550,990 (GRCm39)		probably benign	Het
Zc3hc1	C	T	6: 30,374,763 (GRCm39)		probably null	Het
Zfhx3	T	C	8: 109,682,664 (GRCm39)	Y3368H	unknown	Het
Zfp263	G	A	16: 3,567,580 (GRCm39)	E632K	probably damaging	Het
Zfp266	A	G	9: 20,412,240 (GRCm39)	L111P	probably damaging	Het
Zfp626	T	A	7: 27,517,587 (GRCm39)	S189R	probably benign	Het
Zfp772	T	C	7: 7,206,699 (GRCm39)	R331G	possibly damaging	Het
Zkscan5	G	A	5: 145,157,889 (GRCm39)	C797Y	probably damaging	Het

Other mutations in Or4f60

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00895:Or4f60	APN	2	111,902,100 (GRCm39)	missense	probably damaging	0.98
IGL01790:Or4f60	APN	2	111,902,266 (GRCm39)	missense	probably benign	0.00
IGL02054:Or4f60	APN	2	111,902,269 (GRCm39)	missense	probably benign	0.43
IGL03387:Or4f60	APN	2	111,902,007 (GRCm39)	missense	probably benign	0.00
R1891:Or4f60	UTSW	2	111,902,739 (GRCm39)	missense	probably damaging	1.00
R2509:Or4f60	UTSW	2	111,902,837 (GRCm39)	missense	probably benign
R2520:Or4f60	UTSW	2	111,902,013 (GRCm39)	missense	probably benign	0.00
R3084:Or4f60	UTSW	2	111,902,320 (GRCm39)	nonsense	probably null
R3086:Or4f60	UTSW	2	111,902,320 (GRCm39)	nonsense	probably null
R4357:Or4f60	UTSW	2	111,902,583 (GRCm39)	missense	probably damaging	1.00
R4700:Or4f60	UTSW	2	111,902,097 (GRCm39)	missense	possibly damaging	0.54
R5366:Or4f60	UTSW	2	111,902,823 (GRCm39)	missense	possibly damaging	0.91
R5372:Or4f60	UTSW	2	111,902,454 (GRCm39)	missense	probably benign	0.03
R5643:Or4f60	UTSW	2	111,902,013 (GRCm39)	missense	probably benign	0.00
R5644:Or4f60	UTSW	2	111,902,013 (GRCm39)	missense	probably benign	0.00
R5681:Or4f60	UTSW	2	111,902,722 (GRCm39)	missense	probably benign	0.38
R6004:Or4f60	UTSW	2	111,902,371 (GRCm39)	missense	probably damaging	1.00
R6609:Or4f60	UTSW	2	111,902,509 (GRCm39)	missense	probably benign	0.01
R7056:Or4f60	UTSW	2	111,902,662 (GRCm39)	missense	probably benign
R7066:Or4f60	UTSW	2	111,902,541 (GRCm39)	missense	probably damaging	1.00
R7417:Or4f60	UTSW	2	111,902,445 (GRCm39)	missense	probably benign	0.01
R8942:Or4f60	UTSW	2	111,902,802 (GRCm39)	missense	probably damaging	1.00
R9261:Or4f60	UTSW	2	111,902,718 (GRCm39)	missense	probably damaging	1.00
R9423:Or4f60	UTSW	2	111,902,808 (GRCm39)	missense	possibly damaging	0.91

Predicted Primers

PCR Primer
(F):5'- CTTATCCAGGTGTGTAGAGGAAG -3'
(R):5'- TACCTGACCATGATGAGCCC -3'

Sequencing Primer
(F):5'- GAAGGCCATGTATAGAAAAATATGGC -3'
(R):5'- ATGATGAGCCCCCAGATGTG -3'

Posted On

2015-02-05