Mutagenetix > Incidental Mutation

Incidental Mutation 'R2971:Ifit3b'

265693

Institutional Source

Beutler Lab

Gene Symbol

Ifit3b

Ensembl Gene

ENSMUSG00000062488

Gene Name

interferon-induced protein with tetratricopeptide repeats 3B

Synonyms

I830012O16Rik

MMRRC Submission

040525-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.054) question?

Stock #

R2971 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

34585370-34590801 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to T at 34589417 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Stop codon at position 198 (Q198*)

Ref Sequence

ENSEMBL: ENSMUSP00000075599 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076249]

AlphaFold

E9PV48

Predicted Effect

probably null
Transcript: ENSMUST00000076249
AA Change: Q198*

SMART Domains

Protein: ENSMUSP00000075599
Gene: ENSMUSG00000062488
AA Change: Q198*

Domain	Start	End	E-Value	Type
TPR	51	84	7.69e1	SMART
TPR	94	127	2.84e1	SMART
TPR	136	169	5.69e0	SMART
Blast:TPR	170	206	5e-6	BLAST
TPR	241	274	1.02e1	SMART

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 94.6%

Validation Efficiency

100% (35/35)

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acan	A	G	7: 78,749,447 (GRCm39)	D1406G	possibly damaging	Het
Aebp2	T	C	6: 140,579,624 (GRCm39)		probably null	Het
Ap5m1	T	A	14: 49,321,339 (GRCm39)	Y49*	probably null	Het
Atp8b5	T	C	4: 43,361,953 (GRCm39)		probably benign	Het
Baz1a	A	G	12: 54,970,224 (GRCm39)	S518P	probably damaging	Het
Ces1c	T	A	8: 93,830,821 (GRCm39)	D445V	probably benign	Het
Ctnnbl1	C	T	2: 157,713,106 (GRCm39)	H464Y	probably benign	Het
Cyp2j6	T	C	4: 96,420,018 (GRCm39)	K238E	probably benign	Het
Gdf10	G	A	14: 33,646,148 (GRCm39)	R99H	probably damaging	Het
Gm4779	G	A	X: 100,836,568 (GRCm39)	P116L	possibly damaging	Het
Gucy2g	A	G	19: 55,198,708 (GRCm39)	S812P	probably damaging	Het
Irgm1	A	T	11: 48,757,417 (GRCm39)	Y131*	probably null	Het
Man1c1	G	C	4: 134,430,749 (GRCm39)	P11R	probably damaging	Het
Myh7b	A	G	2: 155,474,175 (GRCm39)	N1630S	probably benign	Het
Myo5a	T	C	9: 75,023,484 (GRCm39)	I15T	probably damaging	Het
Naip6	C	T	13: 100,437,108 (GRCm39)	A472T	probably benign	Het
Nme6	A	G	9: 109,671,159 (GRCm39)		probably benign	Het
Or11g25	T	A	14: 50,723,065 (GRCm39)	I50N	probably damaging	Het
Or5al6	T	C	2: 85,976,908 (GRCm39)	T57A	probably damaging	Het
Plch2	T	G	4: 155,075,224 (GRCm39)	M797L	probably benign	Het
Plscr2	G	T	9: 92,172,724 (GRCm39)	E128*	probably null	Het
Plxna2	T	A	1: 194,480,039 (GRCm39)	D1403E	probably damaging	Het
Pou6f2	T	C	13: 18,556,552 (GRCm39)	T25A	unknown	Het
Psmb11	T	C	14: 54,862,800 (GRCm39)	V6A	possibly damaging	Het
Ptch1	T	G	13: 63,672,773 (GRCm39)	E944A	probably benign	Het
Ptprd	T	G	4: 76,025,561 (GRCm39)	S546R	probably benign	Het
Rbp3	A	G	14: 33,676,411 (GRCm39)	N120D	probably benign	Het
Skint1	C	A	4: 111,878,527 (GRCm39)	P153H	possibly damaging	Het
Slc15a4	A	G	5: 127,681,600 (GRCm39)		probably null	Het
Tmem201	A	G	4: 149,806,902 (GRCm39)		probably benign	Het
Ube2v1	T	C	2: 167,452,256 (GRCm39)	N89D	probably damaging	Het
Zfp282	A	T	6: 47,874,866 (GRCm39)		probably null	Het
Zfp560	C	A	9: 20,260,240 (GRCm39)	M207I	probably benign	Het
Zfp697	T	C	3: 98,335,617 (GRCm39)	Y461H	probably damaging	Het

Other mutations in Ifit3b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
Galilee	UTSW	19	34,588,925 (GRCm39)	missense	probably benign
negev	UTSW	19	34,588,860 (GRCm39)	missense	probably benign	0.14
R1528:Ifit3b	UTSW	19	34,589,072 (GRCm39)	missense	probably benign	0.05
R1996:Ifit3b	UTSW	19	34,588,877 (GRCm39)	missense	probably damaging	1.00
R2680:Ifit3b	UTSW	19	34,589,705 (GRCm39)	missense	probably benign	0.01
R4395:Ifit3b	UTSW	19	34,589,951 (GRCm39)	nonsense	probably null
R4719:Ifit3b	UTSW	19	34,590,030 (GRCm39)	missense	probably damaging	0.96
R4726:Ifit3b	UTSW	19	34,588,860 (GRCm39)	missense	probably benign	0.14
R5094:Ifit3b	UTSW	19	34,589,948 (GRCm39)	missense	possibly damaging	0.93
R5958:Ifit3b	UTSW	19	34,589,142 (GRCm39)	missense	probably benign	0.02
R5987:Ifit3b	UTSW	19	34,589,598 (GRCm39)	missense	probably damaging	1.00
R6381:Ifit3b	UTSW	19	34,589,871 (GRCm39)	missense	probably benign	0.00
R6614:Ifit3b	UTSW	19	34,588,919 (GRCm39)	missense	probably benign	0.01
R6662:Ifit3b	UTSW	19	34,589,337 (GRCm39)	missense	probably damaging	1.00
R6804:Ifit3b	UTSW	19	34,588,947 (GRCm39)	missense	possibly damaging	0.92
R6847:Ifit3b	UTSW	19	34,588,925 (GRCm39)	missense	probably benign
R7685:Ifit3b	UTSW	19	34,589,955 (GRCm39)	missense	possibly damaging	0.94

Predicted Primers

PCR Primer
(F):5'- TGCCCAGAACTTGAATGTGAG -3'
(R):5'- ATCGCAGTGCTTTTCCAAGTAAC -3'

Sequencing Primer
(F):5'- CCCAGAACTTGAATGTGAGGAAGG -3'
(R):5'- AACTCAATAGCTCTGTCTAGGTTACC -3'

Posted On

2015-02-05