Incidental Mutation 'R3622:Midn'
ID 268681
Institutional Source Beutler Lab
Gene Symbol Midn
Ensembl Gene ENSMUSG00000035621
Gene Name midnolin
Synonyms 3000003C15Rik
MMRRC Submission 040677-MU
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R3622 (G1)
Quality Score 153
Status Not validated
Chromosome 10
Chromosomal Location 79984106-79994202 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 79986144 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Aspartic acid to Glycine at position 78 (D78G)
Ref Sequence ENSEMBL: ENSMUSP00000097091 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000003156] [ENSMUST00000042057] [ENSMUST00000099492] [ENSMUST00000105366] [ENSMUST00000105367] [ENSMUST00000151202] [ENSMUST00000144526] [ENSMUST00000146516] [ENSMUST00000153477]
AlphaFold Q3TPJ7
Predicted Effect probably benign
Transcript: ENSMUST00000003156
SMART Domains Protein: ENSMUSP00000003156
Gene: ENSMUSG00000003072

DomainStartEndE-ValueType
low complexity region 23 36 N/A INTRINSIC
Pfam:ATP-synt_DE_N 38 119 2.2e-21 PFAM
low complexity region 144 162 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000042057
AA Change: D78G

PolyPhen 2 Score 0.160 (Sensitivity: 0.92; Specificity: 0.87)
SMART Domains Protein: ENSMUSP00000046967
Gene: ENSMUSG00000035621
AA Change: D78G

DomainStartEndE-ValueType
UBQ 32 102 3.39e-7 SMART
low complexity region 130 143 N/A INTRINSIC
low complexity region 197 211 N/A INTRINSIC
low complexity region 238 262 N/A INTRINSIC
low complexity region 283 302 N/A INTRINSIC
low complexity region 398 409 N/A INTRINSIC
low complexity region 434 453 N/A INTRINSIC
low complexity region 465 485 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000099492
AA Change: D78G

PolyPhen 2 Score 0.160 (Sensitivity: 0.92; Specificity: 0.87)
SMART Domains Protein: ENSMUSP00000097091
Gene: ENSMUSG00000035621
AA Change: D78G

DomainStartEndE-ValueType
UBQ 32 102 3.39e-7 SMART
low complexity region 154 168 N/A INTRINSIC
low complexity region 195 219 N/A INTRINSIC
low complexity region 240 259 N/A INTRINSIC
low complexity region 355 366 N/A INTRINSIC
low complexity region 391 410 N/A INTRINSIC
low complexity region 422 442 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000105366
SMART Domains Protein: ENSMUSP00000101005
Gene: ENSMUSG00000003072

DomainStartEndE-ValueType
low complexity region 23 36 N/A INTRINSIC
Pfam:ATP-synt_DE_N 38 103 2.3e-17 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000105367
SMART Domains Protein: ENSMUSP00000101006
Gene: ENSMUSG00000003072

DomainStartEndE-ValueType
low complexity region 23 36 N/A INTRINSIC
Pfam:ATP-synt_DE_N 38 119 2.2e-21 PFAM
low complexity region 144 162 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000124179
Predicted Effect probably benign
Transcript: ENSMUST00000151202
SMART Domains Protein: ENSMUSP00000115717
Gene: ENSMUSG00000035621

DomainStartEndE-ValueType
Blast:UBQ 32 67 4e-18 BLAST
Predicted Effect probably benign
Transcript: ENSMUST00000144526
SMART Domains Protein: ENSMUSP00000120988
Gene: ENSMUSG00000035621

DomainStartEndE-ValueType
low complexity region 23 36 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000146516
SMART Domains Protein: ENSMUSP00000119962
Gene: ENSMUSG00000035621

DomainStartEndE-ValueType
low complexity region 47 61 N/A INTRINSIC
low complexity region 88 112 N/A INTRINSIC
low complexity region 133 152 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000153477
SMART Domains Protein: ENSMUSP00000119787
Gene: ENSMUSG00000035621

DomainStartEndE-ValueType
low complexity region 23 36 N/A INTRINSIC
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.5%
  • 10x: 97.2%
  • 20x: 95.1%
Validation Efficiency 100% (42/42)
MGI Phenotype FUNCTION: This gene encodes a protein that contains an ubiquitin-like domain. This protein may be involved in the regulation of brain development as inferred by its high expression level in the embryonic midbrain. This protein has been found to negatively regulate glucokinase activity and insulin secretion in pancreatic beta cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
PHENOTYPE: Mice homozygous for a transgenic gene disruption exhibit cleft palate and small ovary. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 42 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca15 T A 7: 119,950,036 (GRCm39) Y503* probably null Het
Akap9 C A 5: 4,026,235 (GRCm39) Q1297K possibly damaging Het
Dffb T C 4: 154,049,976 (GRCm39) T296A probably damaging Het
Dpf1 G A 7: 29,015,631 (GRCm39) probably null Het
Elp1 A T 4: 56,759,925 (GRCm39) probably null Het
Grid2ip T C 5: 143,371,774 (GRCm39) S666P probably damaging Het
Gucy2e T A 11: 69,115,877 (GRCm39) E835V probably damaging Het
Hdac5 G A 11: 102,086,644 (GRCm39) P120S probably benign Het
Htr1d T C 4: 136,170,815 (GRCm39) I348T probably damaging Het
Hyal4 A T 6: 24,765,737 (GRCm39) S364C probably damaging Het
Igkv1-133 A T 6: 67,701,944 (GRCm39) Q16L probably benign Het
Itga10 C T 3: 96,559,054 (GRCm39) probably benign Het
Met A T 6: 17,549,085 (GRCm39) D979V probably damaging Het
Mga A G 2: 119,772,245 (GRCm39) T1702A probably damaging Het
Muc5b T C 7: 141,405,595 (GRCm39) probably benign Het
Oma1 T C 4: 103,223,288 (GRCm39) I491T probably benign Het
Or1e30 A G 11: 73,678,567 (GRCm39) T268A probably benign Het
Or5ak24 C T 2: 85,260,837 (GRCm39) C112Y probably benign Het
Or5b107 T A 19: 13,143,020 (GRCm39) M214K probably benign Het
Or8b48 A G 9: 38,492,792 (GRCm39) Y73C probably damaging Het
Pbld2 T C 10: 62,897,470 (GRCm39) L57P probably damaging Het
Phka2 T A X: 159,327,291 (GRCm39) Y334* probably null Het
Plin4 G T 17: 56,411,112 (GRCm39) T973K possibly damaging Het
R3hdm4 C T 10: 79,748,515 (GRCm39) R143H possibly damaging Het
Rps18 G C 17: 34,171,247 (GRCm39) probably null Het
Samd9l A T 6: 3,374,032 (GRCm39) C1076* probably null Het
Scml4 T C 10: 42,806,607 (GRCm39) probably benign Het
Slc16a10 A G 10: 40,017,890 (GRCm39) V48A probably benign Het
Slc6a5 T C 7: 49,567,371 (GRCm39) V275A probably benign Het
Smad9 C T 3: 54,696,705 (GRCm39) R257W probably damaging Het
Snrpb C A 2: 130,017,299 (GRCm39) R73L probably null Het
Srsf9 C T 5: 115,468,571 (GRCm39) A69V probably damaging Het
Stfa2 A G 16: 36,224,433 (GRCm39) Y90H probably damaging Het
Tgm6 T A 2: 129,993,681 (GRCm39) V640E possibly damaging Het
Tnrc6c C T 11: 117,640,451 (GRCm39) R1414C probably damaging Het
Tyk2 A T 9: 21,038,606 (GRCm39) C8S probably damaging Het
Upp2 G A 2: 58,680,128 (GRCm39) R300Q possibly damaging Het
Utp20 A G 10: 88,593,855 (GRCm39) probably benign Het
Veph1 C T 3: 66,122,858 (GRCm39) V224I probably benign Het
Vmn1r30 A T 6: 58,412,437 (GRCm39) F132I probably benign Het
Vmn2r116 A G 17: 23,605,025 (GRCm39) S113G probably benign Het
Vps53 A C 11: 76,008,609 (GRCm39) V237G probably benign Het
Other mutations in Midn
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01586:Midn APN 10 79,992,477 (GRCm39) unclassified probably benign
IGL01969:Midn APN 10 79,991,093 (GRCm39) missense probably benign 0.00
IGL02824:Midn APN 10 79,989,486 (GRCm39) missense possibly damaging 0.91
Dunkel UTSW 10 79,989,918 (GRCm39) missense probably damaging 0.96
full_moon UTSW 10 79,985,946 (GRCm39) missense possibly damaging 0.66
Midnight UTSW 10 79,990,291 (GRCm39) missense probably damaging 0.98
Sepia UTSW 10 79,987,238 (GRCm39) missense probably null 0.26
R0684:Midn UTSW 10 79,992,336 (GRCm39) missense probably damaging 1.00
R1517:Midn UTSW 10 79,989,957 (GRCm39) missense probably damaging 0.96
R1926:Midn UTSW 10 79,987,495 (GRCm39) missense probably damaging 1.00
R2004:Midn UTSW 10 79,990,983 (GRCm39) missense probably benign 0.13
R2016:Midn UTSW 10 79,985,949 (GRCm39) missense possibly damaging 0.91
R2340:Midn UTSW 10 79,985,946 (GRCm39) missense possibly damaging 0.66
R2483:Midn UTSW 10 79,986,144 (GRCm39) missense probably benign 0.16
R3624:Midn UTSW 10 79,986,144 (GRCm39) missense probably benign 0.16
R4296:Midn UTSW 10 79,987,553 (GRCm39) missense probably damaging 1.00
R4740:Midn UTSW 10 79,987,238 (GRCm39) missense probably null 0.26
R4930:Midn UTSW 10 79,991,189 (GRCm39) missense probably benign
R4977:Midn UTSW 10 79,986,018 (GRCm39) missense probably damaging 1.00
R5423:Midn UTSW 10 79,991,027 (GRCm39) missense probably benign 0.15
R6149:Midn UTSW 10 79,990,291 (GRCm39) missense probably damaging 0.98
R6542:Midn UTSW 10 79,992,418 (GRCm39) missense probably damaging 0.97
R6826:Midn UTSW 10 79,989,961 (GRCm39) nonsense probably null
R7478:Midn UTSW 10 79,991,156 (GRCm39) missense possibly damaging 0.53
R8025:Midn UTSW 10 79,991,126 (GRCm39) missense probably benign 0.00
R8819:Midn UTSW 10 79,990,234 (GRCm39) missense probably damaging 1.00
R8820:Midn UTSW 10 79,990,234 (GRCm39) missense probably damaging 1.00
R8870:Midn UTSW 10 79,985,939 (GRCm39) missense probably damaging 0.96
R9040:Midn UTSW 10 79,989,918 (GRCm39) missense probably damaging 0.96
R9228:Midn UTSW 10 79,990,275 (GRCm39) missense probably damaging 1.00
R9399:Midn UTSW 10 79,992,210 (GRCm39) nonsense probably null
R9784:Midn UTSW 10 79,992,247 (GRCm39) missense probably damaging 1.00
X0018:Midn UTSW 10 79,989,831 (GRCm39) missense possibly damaging 0.90
Z1176:Midn UTSW 10 79,989,462 (GRCm39) missense probably benign
Z1177:Midn UTSW 10 79,986,074 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- AGTCCCGGCTAGGATTGGAG -3'
(R):5'- GCTCCTGCCTTTGTGCAGAC -3'

Sequencing Primer
(F):5'- TAGGATTGGAGGCGACCC -3'
(R):5'- GGCCCAAGCAAGTAAGGCC -3'
Posted On 2015-02-19