Mutagenetix > Incidental Mutation

Incidental Mutation 'R3693:Tas2r143'

268855

Institutional Source

Beutler Lab

Gene Symbol

Tas2r143

Ensembl Gene

ENSMUSG00000046652

Gene Name

taste receptor, type 2, member 143

Synonyms

Tas2r43, mt2r36

MMRRC Submission

040688-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.048) question?

Stock #

R3693 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

42377172-42378053 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 42377910 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Isoleucine at position 247 (V247I)

Ref Sequence

ENSEMBL: ENSMUSP00000057910 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000057398] [ENSMUST00000070178]

AlphaFold

Q7TQB9

Predicted Effect

probably benign
Transcript: ENSMUST00000057398
AA Change: V247I

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000057910
Gene: ENSMUSG00000046652
AA Change: V247I

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	293	6.7e-68	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000070178

SMART Domains

Protein: ENSMUSP00000070247
Gene: ENSMUSG00000056203

Domain	Start	End	E-Value	Type
Pfam:TAS2R	22	320	1.3e-63	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.2%
20x: 94.9%

Validation Efficiency

97% (37/38)

Allele List at MGI

Other mutations in this stock

Total: 36 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2010106E10Rik	A	T	X: 111,466,012 (GRCm39)	L187F	probably damaging	Het
Ankrd29	G	A	18: 12,387,757 (GRCm39)	A275V	possibly damaging	Het
Cabp2	A	G	19: 4,133,593 (GRCm39)	T12A	probably benign	Het
Ccdc158	T	C	5: 92,757,904 (GRCm39)	E1056G	probably damaging	Het
Cdhr2	A	G	13: 54,874,229 (GRCm39)	Y767C	probably damaging	Het
Ces2e	A	G	8: 105,655,443 (GRCm39)	D122G	probably damaging	Het
Cherp	TTGCTGCTGCTGCTGCTGCTG	TTGCTGCTGCTGCTGCTG	8: 73,221,755 (GRCm39)		probably benign	Het
Chsy3	A	T	18: 59,309,080 (GRCm39)	Q111L	possibly damaging	Het
Crygs	C	T	16: 22,624,301 (GRCm39)	G102D	possibly damaging	Het
Cyp11b2	C	T	15: 74,727,857 (GRCm39)	R75Q	probably benign	Het
Dnaja2	T	C	8: 86,273,249 (GRCm39)	K223E	probably damaging	Het
Dtx3	T	C	10: 127,027,293 (GRCm39)	I294V	probably benign	Het
Eps15l1	A	G	8: 73,152,904 (GRCm39)		probably benign	Het
Exd2	A	G	12: 80,527,467 (GRCm39)	Y220C	probably damaging	Het
Eya1	A	G	1: 14,299,725 (GRCm39)	Y343H	probably damaging	Het
Hif3a	T	C	7: 16,774,999 (GRCm39)	E533G	probably damaging	Het
Kalrn	T	A	16: 34,177,685 (GRCm39)	Y178F	probably damaging	Het
Mageb4	G	T	X: 85,296,000 (GRCm39)	R10S	probably damaging	Het
Muc6	A	G	7: 141,234,946 (GRCm39)		probably benign	Het
Myh11	C	A	16: 14,035,813 (GRCm39)	E1038D	probably benign	Het
Nfxl1	T	C	5: 72,697,954 (GRCm39)	Y297C	probably damaging	Het
Nop14	T	C	5: 34,811,782 (GRCm39)	K202E	probably damaging	Het
Or5b112	T	C	19: 13,319,893 (GRCm39)	I257T	possibly damaging	Het
Osbpl8	T	A	10: 111,105,297 (GRCm39)	S251T	probably benign	Het
Pcdha1	A	T	18: 37,065,361 (GRCm39)	K675I	possibly damaging	Het
Pigw	A	G	11: 84,769,209 (GRCm39)	I40T	probably benign	Het
Pip5k1a	A	T	3: 94,985,498 (GRCm39)		probably benign	Het
Ptprh	G	T	7: 4,557,234 (GRCm39)	Q687K	probably damaging	Het
Rbm4	A	G	19: 4,837,411 (GRCm39)	Y358H	probably damaging	Het
Rft1	T	A	14: 30,412,408 (GRCm39)	C490S	probably damaging	Het
Rims2	A	T	15: 39,341,971 (GRCm39)	T887S	probably benign	Het
Ripk2	A	T	4: 16,127,695 (GRCm39)	L349Q	probably benign	Het
Strn	T	C	17: 78,964,421 (GRCm39)	N515D	probably damaging	Het
Stxbp5l	A	T	16: 37,061,708 (GRCm39)	Y367*	probably null	Het
Togaram1	A	T	12: 65,030,283 (GRCm39)	Y1029F	probably benign	Het
Ugt1a6b	A	G	1: 88,035,516 (GRCm39)	T285A	probably benign	Het

Other mutations in Tas2r143

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02216:Tas2r143	APN	6	42,377,268 (GRCm39)	nonsense	probably null
IGL02832:Tas2r143	APN	6	42,377,259 (GRCm39)	missense	possibly damaging	0.55
R0125:Tas2r143	UTSW	6	42,377,889 (GRCm39)	missense	probably benign	0.01
R1035:Tas2r143	UTSW	6	42,377,199 (GRCm39)	missense	probably benign	0.16
R1073:Tas2r143	UTSW	6	42,377,694 (GRCm39)	missense	probably benign	0.01
R1400:Tas2r143	UTSW	6	42,377,317 (GRCm39)	missense	probably benign	0.35
R1774:Tas2r143	UTSW	6	42,377,305 (GRCm39)	missense	probably damaging	1.00
R2391:Tas2r143	UTSW	6	42,377,810 (GRCm39)	missense	probably damaging	0.99
R3617:Tas2r143	UTSW	6	42,377,997 (GRCm39)	missense	probably benign	0.20
R4283:Tas2r143	UTSW	6	42,378,007 (GRCm39)	splice site	probably null
R4486:Tas2r143	UTSW	6	42,377,628 (GRCm39)	missense	probably benign	0.15
R5005:Tas2r143	UTSW	6	42,377,658 (GRCm39)	missense	probably benign	0.02
R6360:Tas2r143	UTSW	6	42,377,769 (GRCm39)	missense	probably benign	0.40
R7163:Tas2r143	UTSW	6	42,377,202 (GRCm39)	missense	probably benign
R7827:Tas2r143	UTSW	6	42,377,656 (GRCm39)	missense	probably damaging	1.00
R8899:Tas2r143	UTSW	6	42,377,888 (GRCm39)	nonsense	probably null
R8989:Tas2r143	UTSW	6	42,377,828 (GRCm39)	missense	probably damaging	1.00
R9264:Tas2r143	UTSW	6	42,377,673 (GRCm39)	missense	probably benign	0.09

Predicted Primers

PCR Primer
(F):5'- GTGCTCATGTGGTTCACTCC -3'
(R):5'- GCAAGCAGAGACACCTTTGGTC -3'

Sequencing Primer
(F):5'- ATGTGGTTCACTCCTTTCTTCCTG -3'
(R):5'- CAGAGACACCTTTGGTCCAAGG -3'

Posted On

2015-02-19