Incidental Mutation 'R3610:Dcaf10'
| ID |
269198 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Dcaf10
|
| Ensembl Gene |
ENSMUSG00000035572 |
| Gene Name |
DDB1 and CUL4 associated factor 10 |
| Synonyms |
Wdr32 |
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R3610 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
4 |
| Chromosomal Location |
45342101-45379759 bp(+) (GRCm39) |
| Type of Mutation |
nonsense |
| DNA Base Change (assembly) |
G to T
at 45372962 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Glutamic Acid to Stop codon
at position 409
(E409*)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000117082
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000153803]
[ENSMUST00000155551]
|
| AlphaFold |
A2AKB9 |
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000107798
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000130821
|
| Predicted Effect |
probably null
Transcript: ENSMUST00000153803
AA Change: E68*
|
| SMART Domains |
Protein: ENSMUSP00000121616
Gene: ENSMUSG00000035572
AA Change: E68*
| Domain | Start | End | E-Value | Type |
|---|
|
Blast:WD40
|
1 |
30 |
3e-11 |
BLAST |
|
Blast:WD40
|
127 |
165 |
2e-10 |
BLAST |
|
WD40
|
183 |
222 |
1.31e-3 |
SMART |
|
| Predicted Effect |
probably null
Transcript: ENSMUST00000155551
AA Change: E409*
|
| SMART Domains |
Protein: ENSMUSP00000117082
Gene: ENSMUSG00000035572
AA Change: E409*
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
15 |
46 |
N/A |
INTRINSIC |
|
low complexity region
|
80 |
107 |
N/A |
INTRINSIC |
|
low complexity region
|
110 |
132 |
N/A |
INTRINSIC |
|
WD40
|
166 |
203 |
1.71e1 |
SMART |
|
WD40
|
206 |
245 |
7.85e-7 |
SMART |
|
WD40
|
249 |
288 |
2.59e-7 |
SMART |
|
WD40
|
295 |
334 |
2.05e1 |
SMART |
|
low complexity region
|
352 |
374 |
N/A |
INTRINSIC |
|
Blast:WD40
|
468 |
506 |
3e-10 |
BLAST |
|
WD40
|
524 |
563 |
1.31e-3 |
SMART |
|
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.6%
- 10x: 97.2%
- 20x: 94.6%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 19 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Acsf2 |
C |
A |
11: 94,452,172 (GRCm39) |
V457L |
probably benign |
Het |
| Cacna1a |
C |
A |
8: 85,285,694 (GRCm39) |
R733S |
probably damaging |
Het |
| Cc2d2a |
A |
T |
5: 43,869,668 (GRCm39) |
E856D |
probably damaging |
Het |
| Cpeb2 |
T |
C |
5: 43,443,276 (GRCm39) |
F989S |
probably damaging |
Het |
| Ddx3y |
T |
C |
Y: 1,263,928 (GRCm39) |
N545S |
probably null |
Het |
| Eef2k |
A |
G |
7: 120,488,458 (GRCm39) |
S448G |
probably benign |
Het |
| Irf2bpl |
T |
C |
12: 86,928,631 (GRCm39) |
I681V |
probably benign |
Het |
| Kremen1 |
CGGG |
CGGGGGG |
11: 5,151,791 (GRCm39) |
|
probably benign |
Het |
| Morc2b |
A |
G |
17: 33,355,252 (GRCm39) |
V840A |
probably damaging |
Het |
| Pgap6 |
G |
A |
17: 26,337,860 (GRCm39) |
V415I |
probably benign |
Het |
| Rcl1 |
A |
G |
19: 29,095,630 (GRCm39) |
T72A |
probably benign |
Het |
| Robo1 |
T |
C |
16: 72,780,658 (GRCm39) |
F796S |
probably benign |
Het |
| Rsf1 |
A |
AAGGCGACGG |
7: 97,229,111 (GRCm39) |
|
probably null |
Het |
| Setx |
GTGGCT |
GT |
2: 29,044,073 (GRCm39) |
1814 |
probably null |
Het |
| Tars1 |
T |
C |
15: 11,392,990 (GRCm39) |
Y152C |
probably damaging |
Het |
| Tmem184a |
A |
G |
5: 139,793,710 (GRCm39) |
|
probably null |
Het |
| Tsc2 |
A |
C |
17: 24,841,524 (GRCm39) |
I365S |
possibly damaging |
Het |
| Usp17la |
G |
A |
7: 104,510,279 (GRCm39) |
V295I |
probably damaging |
Het |
| Vim |
A |
T |
2: 13,583,437 (GRCm39) |
H253L |
possibly damaging |
Het |
|
| Other mutations in Dcaf10 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL02431:Dcaf10
|
APN |
4 |
45,342,630 (GRCm39) |
missense |
probably benign |
0.01 |
|
IGL02660:Dcaf10
|
APN |
4 |
45,372,769 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R0048:Dcaf10
|
UTSW |
4 |
45,374,262 (GRCm39) |
nonsense |
probably null |
|
|
R0550:Dcaf10
|
UTSW |
4 |
45,372,753 (GRCm39) |
missense |
probably benign |
|
|
R0611:Dcaf10
|
UTSW |
4 |
45,373,011 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2289:Dcaf10
|
UTSW |
4 |
45,359,816 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2973:Dcaf10
|
UTSW |
4 |
45,373,957 (GRCm39) |
missense |
probably benign |
0.04 |
|
R3735:Dcaf10
|
UTSW |
4 |
45,348,117 (GRCm39) |
missense |
probably benign |
0.01 |
|
R4655:Dcaf10
|
UTSW |
4 |
45,372,769 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R4665:Dcaf10
|
UTSW |
4 |
45,372,769 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R4690:Dcaf10
|
UTSW |
4 |
45,372,769 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R4724:Dcaf10
|
UTSW |
4 |
45,372,769 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R4725:Dcaf10
|
UTSW |
4 |
45,372,769 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R4735:Dcaf10
|
UTSW |
4 |
45,372,769 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R4743:Dcaf10
|
UTSW |
4 |
45,370,409 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R5220:Dcaf10
|
UTSW |
4 |
45,373,909 (GRCm39) |
missense |
possibly damaging |
0.94 |
|
R5254:Dcaf10
|
UTSW |
4 |
45,370,415 (GRCm39) |
missense |
possibly damaging |
0.94 |
|
R5855:Dcaf10
|
UTSW |
4 |
45,342,558 (GRCm39) |
missense |
probably benign |
0.18 |
|
R6833:Dcaf10
|
UTSW |
4 |
45,373,043 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7132:Dcaf10
|
UTSW |
4 |
45,342,391 (GRCm39) |
missense |
probably benign |
|
|
R7345:Dcaf10
|
UTSW |
4 |
45,342,583 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R7366:Dcaf10
|
UTSW |
4 |
45,373,919 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7832:Dcaf10
|
UTSW |
4 |
45,348,196 (GRCm39) |
missense |
probably damaging |
1.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- ACATGTCTCGAGCCTCTCAAC -3'
(R):5'- TTACCAGCCTAAGGTCTGAAC -3'
Sequencing Primer
(F):5'- GTCTCGAGCCTCTCAACGAGAAG -3'
(R):5'- GCCTAAGGTCTGAACATGGACATC -3'
|
| Posted On |
2015-02-19 |
Incidental Mutation