Mutagenetix > Incidental Mutation

Incidental Mutation 'R3686:Wdr86'

269520

Institutional Source

Beutler Lab

Gene Symbol

Wdr86

Ensembl Gene

ENSMUSG00000055235

Gene Name

WD repeat domain 86

Synonyms

2810046M22Rik

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.072) question?

Stock #

R3686 (G1)

Quality Score

220

Status

Not validated

Chromosome

Chromosomal Location

24916736-24935725 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 24923339 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 118 (T118S)

Ref Sequence

ENSEMBL: ENSMUSP00000064785 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000068693]

AlphaFold

D3Z757

Predicted Effect

probably damaging
Transcript: ENSMUST00000068693
AA Change: T118S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000064785
Gene: ENSMUSG00000055235
AA Change: T118S

Domain	Start	End	E-Value	Type
WD40	4	43	1.62e-8	SMART
WD40	46	83	3.17e-2	SMART
WD40	86	123	1.9e-5	SMART
WD40	126	179	3e-3	SMART
WD40	182	221	2.78e-7	SMART
WD40	224	261	9.9e-4	SMART
WD40	264	301	1.29e-2	SMART
WD40	304	341	6.28e-6	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000127320

SMART Domains

Protein: ENSMUSP00000119649
Gene: ENSMUSG00000055235

Domain	Start	End	E-Value	Type
WD40	29	63	1.27e2	SMART
WD40	66	103	6.28e-6	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142607

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000196443

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.2%
20x: 94.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Asb16	T	A	11: 102,159,885 (GRCm39)	D79E	probably benign	Het
Atp4a	G	A	7: 30,419,650 (GRCm39)	R671Q	probably benign	Het
B230307C23Rik	T	A	16: 97,810,199 (GRCm39)	N62K	probably benign	Het
B3galnt2	G	A	13: 14,150,220 (GRCm39)		probably null	Het
Bnip2	T	A	9: 69,906,432 (GRCm39)	Y118N	probably damaging	Het
Bptf	C	A	11: 106,965,024 (GRCm39)	R1275L	probably benign	Het
Cacna1g	T	C	11: 94,349,916 (GRCm39)		probably null	Het
Cdh15	G	A	8: 123,588,763 (GRCm39)	R279Q	probably damaging	Het
Cdh4	T	C	2: 179,422,160 (GRCm39)	S95P	probably benign	Het
Ceacam5	G	A	7: 17,494,748 (GRCm39)	E919K	possibly damaging	Het
Eftud2	T	C	11: 102,735,027 (GRCm39)	E624G	probably damaging	Het
Hr	T	A	14: 70,795,236 (GRCm39)	N289K	probably damaging	Het
Hsp90ab1	A	G	17: 45,880,214 (GRCm39)	Y110H	probably damaging	Het
Map3k1	A	G	13: 111,890,425 (GRCm39)	V1258A	probably damaging	Het
Naa11	A	T	5: 97,539,648 (GRCm39)	V170E	probably benign	Het
Nmd3	C	T	3: 69,654,095 (GRCm39)	R413C	probably damaging	Het
Or4k51	G	T	2: 111,584,914 (GRCm39)	V107F	probably benign	Het
Or56b2	A	G	7: 104,337,599 (GRCm39)	I126V	probably benign	Het
Pgm3	G	T	9: 86,441,563 (GRCm39)	P345T	probably benign	Het
Ptpn14	C	A	1: 189,583,596 (GRCm39)	D814E	probably damaging	Het
Rab36	G	A	10: 74,880,328 (GRCm39)	V63I	probably damaging	Het
Rap1gap2	C	A	11: 74,298,148 (GRCm39)	A491S	possibly damaging	Het
Ros1	A	T	10: 52,021,912 (GRCm39)	V624E	probably damaging	Het
Sgo2b	T	C	8: 64,384,361 (GRCm39)	K212E	probably benign	Het
Shisa2	T	A	14: 59,867,228 (GRCm39)	L160Q	probably damaging	Het
Strn4	A	G	7: 16,556,506 (GRCm39)	Y123C	probably damaging	Het
Sycp2	T	C	2: 178,016,177 (GRCm39)	T762A	probably benign	Het
Tiam2	A	G	17: 3,471,959 (GRCm39)	K534E	possibly damaging	Het
Trdn	A	T	10: 33,344,185 (GRCm39)	D633V	probably benign	Het
Ttn	T	C	2: 76,747,333 (GRCm39)	Y4572C	possibly damaging	Het
Unc79	T	A	12: 103,054,920 (GRCm39)	N921K	probably damaging	Het
Vmn2r66	A	G	7: 84,644,397 (GRCm39)	V671A	probably damaging	Het
Zfp446	T	A	7: 12,716,580 (GRCm39)	I517N	probably damaging	Het
Zfp830	G	A	11: 82,656,188 (GRCm39)	E331K	possibly damaging	Het

Other mutations in Wdr86

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02419:Wdr86	APN	5	24,927,702 (GRCm39)	missense	probably damaging	1.00
R0412:Wdr86	UTSW	5	24,923,232 (GRCm39)	missense	probably benign	0.01
R0899:Wdr86	UTSW	5	24,923,005 (GRCm39)	missense	probably benign	0.15
R2520:Wdr86	UTSW	5	24,917,573 (GRCm39)	makesense	probably null
R3522:Wdr86	UTSW	5	24,923,305 (GRCm39)	missense	probably benign	0.00
R4439:Wdr86	UTSW	5	24,935,235 (GRCm39)	missense	probably damaging	1.00
R4657:Wdr86	UTSW	5	24,923,229 (GRCm39)	missense	probably benign	0.00
R4989:Wdr86	UTSW	5	24,917,843 (GRCm39)	splice site	probably null
R5649:Wdr86	UTSW	5	24,923,085 (GRCm39)	missense	probably benign
R5940:Wdr86	UTSW	5	24,927,660 (GRCm39)	missense	probably damaging	1.00
R6254:Wdr86	UTSW	5	24,923,281 (GRCm39)	missense	probably benign	0.04
R8012:Wdr86	UTSW	5	24,935,177 (GRCm39)	critical splice donor site	probably null
R8395:Wdr86	UTSW	5	24,935,187 (GRCm39)	missense	probably damaging	1.00
R9497:Wdr86	UTSW	5	24,920,538 (GRCm39)	missense	probably benign	0.01
Z1177:Wdr86	UTSW	5	24,917,746 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCATCTGAGCTGCCAGTCAC -3'
(R):5'- GTCAGGCCAACAGGTTTGTC -3'

Sequencing Primer
(F):5'- TGAGCTGCCAGTCACTAGGAG -3'
(R):5'- AACTGGATCTTGGAAATTGAGTG -3'

Posted On

2015-02-19