Mutagenetix > Incidental Mutation

Incidental Mutation 'R3739:Vmn1r197'

270308

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r197

Ensembl Gene

ENSMUSG00000069294

Gene Name

vomeronasal 1 receptor 197

Synonyms

V1rh21

MMRRC Submission

040725-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.064) question?

Stock #

R3739 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

22512081-22512977 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to A at 22512746 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 222 (Y222*)

Ref Sequence

ENSEMBL: ENSMUSP00000154632 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000091734] [ENSMUST00000226225] [ENSMUST00000227017]

AlphaFold

Q8R265

Predicted Effect

probably null
Transcript: ENSMUST00000091734
AA Change: Y222*

SMART Domains

Protein: ENSMUSP00000089328
Gene: ENSMUSG00000069294
AA Change: Y222*

Domain	Start	End	E-Value	Type
transmembrane domain	10	29	N/A	INTRINSIC
Pfam:V1R	31	297	1.4e-40	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000226225
AA Change: Y222*

Predicted Effect

probably null
Transcript: ENSMUST00000227017
AA Change: Y222*

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.0%

Validation Efficiency

98% (49/50)

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9930111J21Rik2	A	T	11: 48,910,108 (GRCm39)	L775*	probably null	Het
Abcb8	A	G	5: 24,605,619 (GRCm39)	S168G	probably benign	Het
Ahnak2	T	C	12: 112,740,992 (GRCm39)	I1027V	probably benign	Het
Alox12e	A	G	11: 70,210,668 (GRCm39)	L318P	probably damaging	Het
Ankrd11	A	G	8: 123,623,454 (GRCm39)		probably benign	Het
Anks1b	T	A	10: 89,869,078 (GRCm39)	I46N	probably damaging	Het
Apoa5	G	C	9: 46,180,415 (GRCm39)	W7S	probably damaging	Het
Arnt2	T	C	7: 83,993,009 (GRCm39)	H177R	probably null	Het
Cacna1c	T	C	6: 118,718,913 (GRCm39)	D220G	probably benign	Het
Dst	C	T	1: 34,307,975 (GRCm39)		probably benign	Het
Eml6	C	A	11: 29,753,137 (GRCm39)	V925L	probably benign	Het
Fbll1	G	A	11: 35,688,505 (GRCm39)	H253Y	possibly damaging	Het
Galnt1	A	G	18: 24,404,712 (GRCm39)	T350A	probably benign	Het
Gbp10	T	A	5: 105,372,324 (GRCm39)	E145D	possibly damaging	Het
Gfm1	T	C	3: 67,364,033 (GRCm39)	I503T	probably damaging	Het
Hmcn2	A	T	2: 31,226,624 (GRCm39)	K200*	probably null	Het
Ifi203	T	A	1: 173,757,040 (GRCm39)		probably benign	Het
Itgbl1	T	C	14: 124,204,090 (GRCm39)	F394L	probably damaging	Het
Itpkc	T	A	7: 26,927,029 (GRCm39)	D295V	possibly damaging	Het
Klra17	T	A	6: 129,850,328 (GRCm39)	I41F	probably benign	Het
Lcorl	A	T	5: 45,891,383 (GRCm39)	N323K	possibly damaging	Het
Ltbp2	A	T	12: 84,851,248 (GRCm39)	C836S	probably damaging	Het
Mfsd2b	A	G	12: 4,920,578 (GRCm39)	S80P	probably damaging	Het
Ms4a18	A	T	19: 10,988,863 (GRCm39)	H164Q	probably damaging	Het
Mybbp1a	A	G	11: 72,339,563 (GRCm39)	H882R	possibly damaging	Het
Myh9	G	A	15: 77,651,012 (GRCm39)	R1612C	probably damaging	Het
Myo18a	A	G	11: 77,736,441 (GRCm39)	D1514G	probably damaging	Het
Nsun2	T	C	13: 69,777,757 (GRCm39)	I441T	probably benign	Het
Ntng1	A	T	3: 109,842,007 (GRCm39)	D255E	probably damaging	Het
Or2y1	A	G	11: 49,386,287 (GRCm39)	D309G	possibly damaging	Het
Osbpl2	G	A	2: 179,803,353 (GRCm39)	R475H	probably damaging	Het
Pclo	A	C	5: 14,730,913 (GRCm39)	K3138N	unknown	Het
Pcsk7	A	G	9: 45,838,057 (GRCm39)	T572A	possibly damaging	Het
Pex11a	C	T	7: 79,389,918 (GRCm39)	R56H	possibly damaging	Het
Pnma8b	C	T	7: 16,680,521 (GRCm39)	H502Y	probably benign	Het
Saa4	T	A	7: 46,379,053 (GRCm39)	N96Y	possibly damaging	Het
Serpinb6d	T	A	13: 33,851,663 (GRCm39)	V140E	probably damaging	Het
Srsf4	C	T	4: 131,627,413 (GRCm39)		probably benign	Het
St18	G	A	1: 6,925,697 (GRCm39)		probably null	Het
Taf3	T	C	2: 9,956,469 (GRCm39)	E566G	possibly damaging	Het
Tnr	T	C	1: 159,750,983 (GRCm39)	S1315P	possibly damaging	Het
Trappc11	T	C	8: 47,967,138 (GRCm39)	E412G	probably damaging	Het
Trpc2	G	A	7: 101,733,711 (GRCm39)	S220N	probably damaging	Het
Trpm7	A	G	2: 126,693,441 (GRCm39)	V48A	probably damaging	Het
Tubb6	A	G	18: 67,535,121 (GRCm39)	Y340C	probably damaging	Het
Vmn2r97	T	C	17: 19,148,413 (GRCm39)	S103P	probably damaging	Het
Zfp362	C	G	4: 128,680,682 (GRCm39)		probably benign	Het
Zfp423	A	G	8: 88,507,972 (GRCm39)	C666R	probably damaging	Het

Other mutations in Vmn1r197

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01459:Vmn1r197	APN	13	22,512,241 (GRCm39)	missense	probably benign	0.07
IGL01944:Vmn1r197	APN	13	22,512,508 (GRCm39)	missense	possibly damaging	0.77
IGL02317:Vmn1r197	APN	13	22,512,073 (GRCm39)	utr 5 prime	probably benign
IGL02487:Vmn1r197	APN	13	22,512,792 (GRCm39)	missense	probably damaging	1.00
R0443:Vmn1r197	UTSW	13	22,512,241 (GRCm39)	missense	possibly damaging	0.90
R1511:Vmn1r197	UTSW	13	22,512,823 (GRCm39)	missense	possibly damaging	0.92
R1542:Vmn1r197	UTSW	13	22,512,520 (GRCm39)	missense	probably benign	0.01
R1617:Vmn1r197	UTSW	13	22,512,498 (GRCm39)	missense	possibly damaging	0.55
R1739:Vmn1r197	UTSW	13	22,512,541 (GRCm39)	missense	possibly damaging	0.80
R4761:Vmn1r197	UTSW	13	22,512,174 (GRCm39)	missense	probably benign	0.00
R5335:Vmn1r197	UTSW	13	22,512,361 (GRCm39)	missense	probably damaging	1.00
R6168:Vmn1r197	UTSW	13	22,512,678 (GRCm39)	missense	possibly damaging	0.65
R8056:Vmn1r197	UTSW	13	22,512,388 (GRCm39)	missense	probably damaging	1.00
R9282:Vmn1r197	UTSW	13	22,512,608 (GRCm39)	missense	possibly damaging	0.92
Z1177:Vmn1r197	UTSW	13	22,512,099 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- CCTTGATCAGCATGAACTTACC -3'
(R):5'- GGGGCTGAGAACTGCATAAC -3'

Sequencing Primer
(F):5'- GATCAGCATGAACTTACCCTATTAC -3'
(R):5'- CCAAGGGTTAGGAACTCCG -3'

Posted On

2015-03-18