Mutagenetix > Incidental Mutation

Incidental Mutation 'R3727:Vmn2r95'

270847

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r95

Ensembl Gene

ENSMUSG00000091631

Gene Name

vomeronasal 2, receptor 95

Synonyms

MMRRC Submission

040718-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.064) question?

Stock #

R3727 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

18644366-18672586 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to T at 18661744 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Stop codon at position 497 (Q497*)

Ref Sequence

ENSEMBL: ENSMUSP00000156117 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166327] [ENSMUST00000232090] [ENSMUST00000232464]

AlphaFold

A0A338P6T0

Predicted Effect

probably null
Transcript: ENSMUST00000166327
AA Change: Q497*

SMART Domains

Protein: ENSMUSP00000126106
Gene: ENSMUSG00000091631
AA Change: Q497*

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	82	462	1.8e-35	PFAM
Pfam:NCD3G	509	562	3.2e-20	PFAM
Pfam:7tm_3	594	830	3.2e-51	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000232090
AA Change: Q497*

Predicted Effect

probably benign
Transcript: ENSMUST00000232464

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 94.6%

Validation Efficiency

100% (39/39)

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ago2	C	T	15: 72,985,706 (GRCm39)	V630M	probably damaging	Het
Alk	T	C	17: 72,208,395 (GRCm39)		probably benign	Het
Atp6v0a1	T	A	11: 100,921,246 (GRCm39)	S243T	probably benign	Het
BC035044	C	A	6: 128,867,822 (GRCm39)	G37*	probably null	Het
C3	G	T	17: 57,514,379 (GRCm39)	N1435K	possibly damaging	Het
Caly	T	C	7: 139,650,417 (GRCm39)	E175G	probably damaging	Het
Ccng2	T	C	5: 93,422,810 (GRCm39)	F330S	probably damaging	Het
Cemip2	G	A	19: 21,822,075 (GRCm39)	A1157T	probably benign	Het
Cyp2c38	A	G	19: 39,380,739 (GRCm39)		probably benign	Het
Cypt1	T	A	X: 16,389,674 (GRCm39)	L128*	probably null	Het
Dmgdh	A	G	13: 93,828,575 (GRCm39)	N239D	probably damaging	Het
Dnah8	C	T	17: 30,958,622 (GRCm39)	Q2155*	probably null	Het
Dpp3	A	G	19: 4,973,213 (GRCm39)	I127T	probably benign	Het
Eps15	G	T	4: 109,227,882 (GRCm39)		probably benign	Het
Exosc10	A	G	4: 148,649,734 (GRCm39)	D388G	probably damaging	Het
Flt3	C	A	5: 147,291,733 (GRCm39)	R572S	probably damaging	Het
Gm44501	T	C	17: 40,887,506 (GRCm39)	F8S	unknown	Het
Hormad2	C	A	11: 4,358,598 (GRCm39)	G270C	probably benign	Het
Ifrd2	C	T	9: 107,468,881 (GRCm39)	R40*	probably null	Het
Ina	G	A	19: 47,004,158 (GRCm39)	R322H	possibly damaging	Het
Kif13b	A	G	14: 65,003,197 (GRCm39)		probably benign	Het
Lrp2	T	C	2: 69,340,773 (GRCm39)	N1034S	probably damaging	Het
Macf1	T	A	4: 123,353,104 (GRCm39)	E1770V	probably damaging	Het
Mpi	T	C	9: 57,452,132 (GRCm39)	I381M	possibly damaging	Het
Nfix	CAAAAA	CAAAA	8: 85,442,876 (GRCm39)		probably null	Het
Nt5c	C	T	11: 115,381,474 (GRCm39)	W185*	probably null	Het
Or2j3	A	T	17: 38,616,310 (GRCm39)	I14N	possibly damaging	Het
Or2y1e	G	A	11: 49,218,622 (GRCm39)	R128H	probably benign	Het
Pcdhga9	G	A	18: 37,871,995 (GRCm39)	R608H	probably benign	Het
Pdzrn3	T	A	6: 101,133,906 (GRCm39)	D441V	probably damaging	Het
Rab42	A	G	4: 132,029,964 (GRCm39)	M86T	probably benign	Het
Slc52a3	C	T	2: 151,847,701 (GRCm39)	P270S	probably benign	Het
Stpg2	T	C	3: 139,004,257 (GRCm39)	F278S	probably damaging	Het
Styxl2	G	A	1: 165,927,075 (GRCm39)	R846C	probably damaging	Het
Tmem240	T	C	4: 155,824,235 (GRCm39)		probably benign	Het
Vmn2r22	A	G	6: 123,627,584 (GRCm39)	L4P	possibly damaging	Het
Wnk1	T	C	6: 119,969,414 (GRCm39)	H347R	probably damaging	Het
Zmym2	A	T	14: 57,156,806 (GRCm39)		probably benign	Het

Other mutations in Vmn2r95

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01024:Vmn2r95	APN	17	18,672,590 (GRCm39)	utr 3 prime	probably benign
IGL01479:Vmn2r95	APN	17	18,664,124 (GRCm39)	missense	probably damaging	1.00
IGL01890:Vmn2r95	APN	17	18,671,737 (GRCm39)	missense	probably damaging	1.00
IGL01986:Vmn2r95	APN	17	18,660,473 (GRCm39)	missense	probably benign	0.06
IGL02113:Vmn2r95	APN	17	18,660,169 (GRCm39)	missense	possibly damaging	0.47
IGL02154:Vmn2r95	APN	17	18,672,248 (GRCm39)	missense	probably benign	0.16
IGL02190:Vmn2r95	APN	17	18,672,038 (GRCm39)	missense	probably benign	0.00
IGL02412:Vmn2r95	APN	17	18,660,218 (GRCm39)	missense	probably damaging	1.00
IGL02550:Vmn2r95	APN	17	18,671,994 (GRCm39)	missense	probably damaging	1.00
IGL02679:Vmn2r95	APN	17	18,664,116 (GRCm39)	missense	probably damaging	1.00
IGL02691:Vmn2r95	APN	17	18,672,120 (GRCm39)	missense	probably benign	0.07
IGL02990:Vmn2r95	APN	17	18,672,298 (GRCm39)	nonsense	probably null
IGL03032:Vmn2r95	APN	17	18,672,575 (GRCm39)	missense	probably benign	0.00
R0416:Vmn2r95	UTSW	17	18,661,664 (GRCm39)	missense	probably damaging	1.00
R0448:Vmn2r95	UTSW	17	18,672,005 (GRCm39)	missense	possibly damaging	0.92
R0514:Vmn2r95	UTSW	17	18,671,844 (GRCm39)	missense	probably benign
R0519:Vmn2r95	UTSW	17	18,659,765 (GRCm39)	missense	probably damaging	1.00
R0539:Vmn2r95	UTSW	17	18,672,362 (GRCm39)	missense	probably damaging	1.00
R1501:Vmn2r95	UTSW	17	18,660,118 (GRCm39)	missense	probably damaging	0.99
R1598:Vmn2r95	UTSW	17	18,672,575 (GRCm39)	missense	probably benign	0.03
R1613:Vmn2r95	UTSW	17	18,660,901 (GRCm39)	splice site	probably benign
R1861:Vmn2r95	UTSW	17	18,672,530 (GRCm39)	missense	probably damaging	1.00
R1921:Vmn2r95	UTSW	17	18,644,575 (GRCm39)	missense	probably benign	0.11
R1986:Vmn2r95	UTSW	17	18,671,805 (GRCm39)	missense	probably benign
R2031:Vmn2r95	UTSW	17	18,659,717 (GRCm39)	missense	possibly damaging	0.94
R2040:Vmn2r95	UTSW	17	18,661,561 (GRCm39)	missense	probably damaging	1.00
R3608:Vmn2r95	UTSW	17	18,660,235 (GRCm39)	missense	possibly damaging	0.47
R3953:Vmn2r95	UTSW	17	18,660,358 (GRCm39)	missense	possibly damaging	0.79
R3955:Vmn2r95	UTSW	17	18,660,358 (GRCm39)	missense	possibly damaging	0.79
R3957:Vmn2r95	UTSW	17	18,660,358 (GRCm39)	missense	possibly damaging	0.79
R4474:Vmn2r95	UTSW	17	18,672,507 (GRCm39)	missense	probably damaging	1.00
R4672:Vmn2r95	UTSW	17	18,672,413 (GRCm39)	missense	probably damaging	1.00
R4850:Vmn2r95	UTSW	17	18,671,915 (GRCm39)	missense	probably damaging	1.00
R5054:Vmn2r95	UTSW	17	18,671,708 (GRCm39)	missense	possibly damaging	0.63
R5178:Vmn2r95	UTSW	17	18,660,337 (GRCm39)	missense	probably benign	0.01
R5980:Vmn2r95	UTSW	17	18,661,624 (GRCm39)	missense	probably benign
R6183:Vmn2r95	UTSW	17	18,664,192 (GRCm39)	missense	probably damaging	0.99
R6276:Vmn2r95	UTSW	17	18,671,732 (GRCm39)	missense	possibly damaging	0.96
R6651:Vmn2r95	UTSW	17	18,660,622 (GRCm39)	missense	probably damaging	1.00
R6682:Vmn2r95	UTSW	17	18,660,489 (GRCm39)	missense	probably damaging	1.00
R6797:Vmn2r95	UTSW	17	18,672,551 (GRCm39)	utr 3 prime	probably benign
R6799:Vmn2r95	UTSW	17	18,659,555 (GRCm39)	missense	probably damaging	1.00
R6849:Vmn2r95	UTSW	17	18,664,182 (GRCm39)	missense	probably damaging	1.00
R6849:Vmn2r95	UTSW	17	18,664,181 (GRCm39)	missense	probably damaging	1.00
R6982:Vmn2r95	UTSW	17	18,672,323 (GRCm39)	missense	probably damaging	1.00
R7203:Vmn2r95	UTSW	17	18,661,577 (GRCm39)	missense	probably benign	0.01
R7226:Vmn2r95	UTSW	17	18,672,245 (GRCm39)	missense	possibly damaging	0.90
R7240:Vmn2r95	UTSW	17	18,672,225 (GRCm39)	missense	probably benign	0.15
R7383:Vmn2r95	UTSW	17	18,660,734 (GRCm39)	missense	probably benign	0.06
R7614:Vmn2r95	UTSW	17	18,660,352 (GRCm39)	missense	probably benign
R7755:Vmn2r95	UTSW	17	18,644,367 (GRCm39)	start codon destroyed	probably null	0.99
R7942:Vmn2r95	UTSW	17	18,660,529 (GRCm39)	missense	possibly damaging	0.74
R8355:Vmn2r95	UTSW	17	18,660,352 (GRCm39)	missense	probably benign
R8455:Vmn2r95	UTSW	17	18,660,352 (GRCm39)	missense	probably benign
R8478:Vmn2r95	UTSW	17	18,672,544 (GRCm39)	missense	probably damaging	1.00
R8547:Vmn2r95	UTSW	17	18,664,161 (GRCm39)	missense	probably damaging	1.00
R8752:Vmn2r95	UTSW	17	18,661,738 (GRCm39)	missense	probably damaging	0.98
R8788:Vmn2r95	UTSW	17	18,671,790 (GRCm39)	missense	probably benign	0.09
R8852:Vmn2r95	UTSW	17	18,664,113 (GRCm39)	missense	possibly damaging	0.95
R9098:Vmn2r95	UTSW	17	18,660,167 (GRCm39)	missense	possibly damaging	0.88
R9202:Vmn2r95	UTSW	17	18,644,394 (GRCm39)	missense	probably benign	0.00
R9244:Vmn2r95	UTSW	17	18,672,189 (GRCm39)	missense	possibly damaging	0.91
R9546:Vmn2r95	UTSW	17	18,661,721 (GRCm39)	missense	probably benign	0.01
R9665:Vmn2r95	UTSW	17	18,660,607 (GRCm39)	missense	probably damaging	0.99
Z1088:Vmn2r95	UTSW	17	18,660,663 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CTTTCCTGAAGCATATTGAAGTGAG -3'
(R):5'- GGGGACTTATTGAACACTGTATTCTTC -3'

Sequencing Primer
(F):5'- TGAGAGACAAAAAGAGTTTAGATTGG -3'
(R):5'- TGAACACTGTATTCTTCCATTGATC -3'

Posted On

2015-03-18