Incidental Mutation 'R3754:Zfp109'
| ID |
271349 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp109
|
| Ensembl Gene |
ENSMUSG00000074283 |
| Gene Name |
zinc finger protein 109 |
| Synonyms |
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.075)
|
| Stock # |
R3754 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
7 |
| Chromosomal Location |
23926997-23936985 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to G
at 23929181 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Methionine to Threonine
at position 76
(M76T)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000146043
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000037448]
[ENSMUST00000206362]
[ENSMUST00000206960]
|
| AlphaFold |
A0A0U1RPC5 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000037448
AA Change: M76T
PolyPhen 2
Score 0.342 (Sensitivity: 0.90; Specificity: 0.89)
|
| SMART Domains |
Protein: ENSMUSP00000045498
Gene: ENSMUSG00000074283
AA Change: M76T
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
8 |
74 |
8.77e-20 |
SMART |
|
ZnF_C2H2
|
283 |
305 |
2.91e-2 |
SMART |
|
ZnF_C2H2
|
311 |
333 |
4.47e-3 |
SMART |
|
ZnF_C2H2
|
339 |
361 |
4.11e-2 |
SMART |
|
ZnF_C2H2
|
367 |
387 |
5.54e1 |
SMART |
|
ZnF_C2H2
|
393 |
415 |
1.98e-4 |
SMART |
|
ZnF_C2H2
|
421 |
443 |
8.67e-1 |
SMART |
|
ZnF_C2H2
|
449 |
471 |
4.87e-4 |
SMART |
|
ZnF_C2H2
|
477 |
499 |
7.49e-5 |
SMART |
|
ZnF_C2H2
|
505 |
527 |
1.47e-3 |
SMART |
|
ZnF_C2H2
|
533 |
555 |
3.21e-4 |
SMART |
|
ZnF_C2H2
|
561 |
583 |
8.47e-4 |
SMART |
|
ZnF_C2H2
|
589 |
611 |
2.57e-3 |
SMART |
|
ZnF_C2H2
|
617 |
639 |
4.4e-2 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000206362
AA Change: M76T
PolyPhen 2
Score 0.342 (Sensitivity: 0.90; Specificity: 0.89)
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000206960
AA Change: M84T
PolyPhen 2
Score 0.035 (Sensitivity: 0.94; Specificity: 0.82)
|
| Coding Region Coverage |
- 1x: 99.0%
- 3x: 98.4%
- 10x: 96.7%
- 20x: 93.1%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 22 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Asap3 |
T |
C |
4: 135,956,766 (GRCm39) |
|
probably null |
Het |
| Atp12a |
G |
A |
14: 56,610,045 (GRCm39) |
V182I |
probably benign |
Het |
| Gad2 |
A |
T |
2: 22,571,352 (GRCm39) |
D430V |
possibly damaging |
Het |
| Hr |
A |
G |
14: 70,805,264 (GRCm39) |
E1002G |
probably damaging |
Het |
| Itpkc |
G |
T |
7: 26,927,857 (GRCm39) |
P19Q |
probably damaging |
Het |
| Lrrc72 |
A |
G |
12: 36,262,567 (GRCm39) |
S42P |
probably benign |
Het |
| Med1 |
A |
G |
11: 98,057,548 (GRCm39) |
V318A |
possibly damaging |
Het |
| Myef2l |
G |
A |
3: 10,153,575 (GRCm39) |
V115I |
possibly damaging |
Het |
| Nek11 |
A |
T |
9: 105,191,917 (GRCm39) |
N164K |
probably damaging |
Het |
| Neurod2 |
A |
G |
11: 98,218,526 (GRCm39) |
S213P |
probably damaging |
Het |
| Nhsl1 |
A |
G |
10: 18,391,782 (GRCm39) |
N179D |
probably damaging |
Het |
| Nr2f2 |
T |
C |
7: 70,007,769 (GRCm39) |
I238V |
probably benign |
Het |
| Rpl37 |
C |
A |
15: 5,146,770 (GRCm39) |
T2K |
possibly damaging |
Het |
| Slf2 |
A |
G |
19: 44,961,676 (GRCm39) |
D1065G |
probably benign |
Het |
| Smim5 |
T |
C |
11: 115,796,549 (GRCm39) |
C57R |
probably damaging |
Het |
| Soat2 |
T |
A |
15: 102,065,513 (GRCm39) |
V236D |
probably damaging |
Het |
| Teddm3 |
C |
T |
16: 20,971,898 (GRCm39) |
D224N |
possibly damaging |
Het |
| Tm2d2 |
G |
A |
8: 25,510,494 (GRCm39) |
V118I |
probably damaging |
Het |
| Ttc22 |
C |
A |
4: 106,496,278 (GRCm39) |
R443S |
probably damaging |
Het |
| Upf1 |
A |
G |
8: 70,792,464 (GRCm39) |
I368T |
probably benign |
Het |
| Xrn1 |
G |
T |
9: 95,849,841 (GRCm39) |
D129Y |
probably damaging |
Het |
| Znrf1 |
G |
A |
8: 112,345,843 (GRCm39) |
V76M |
probably damaging |
Het |
|
| Other mutations in Zfp109 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00646:Zfp109
|
APN |
7 |
23,928,237 (GRCm39) |
nonsense |
probably null |
|
|
IGL01082:Zfp109
|
APN |
7 |
23,933,784 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02129:Zfp109
|
APN |
7 |
23,936,054 (GRCm39) |
missense |
possibly damaging |
0.76 |
|
PIT4585001:Zfp109
|
UTSW |
7 |
23,928,779 (GRCm39) |
missense |
probably benign |
0.35 |
|
R0319:Zfp109
|
UTSW |
7 |
23,933,895 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1502:Zfp109
|
UTSW |
7 |
23,927,588 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1912:Zfp109
|
UTSW |
7 |
23,927,676 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R1930:Zfp109
|
UTSW |
7 |
23,928,161 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1931:Zfp109
|
UTSW |
7 |
23,928,161 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1994:Zfp109
|
UTSW |
7 |
23,928,743 (GRCm39) |
missense |
probably benign |
0.04 |
|
R2105:Zfp109
|
UTSW |
7 |
23,936,041 (GRCm39) |
critical splice donor site |
probably null |
|
|
R2219:Zfp109
|
UTSW |
7 |
23,927,886 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2353:Zfp109
|
UTSW |
7 |
23,928,806 (GRCm39) |
missense |
probably benign |
0.06 |
|
R4434:Zfp109
|
UTSW |
7 |
23,928,771 (GRCm39) |
missense |
probably benign |
0.02 |
|
R4884:Zfp109
|
UTSW |
7 |
23,928,570 (GRCm39) |
missense |
probably benign |
0.06 |
|
R5056:Zfp109
|
UTSW |
7 |
23,928,162 (GRCm39) |
missense |
possibly damaging |
0.75 |
|
R5220:Zfp109
|
UTSW |
7 |
23,928,179 (GRCm39) |
missense |
probably benign |
0.03 |
|
R5472:Zfp109
|
UTSW |
7 |
23,928,046 (GRCm39) |
nonsense |
probably null |
|
|
R5715:Zfp109
|
UTSW |
7 |
23,928,995 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R5801:Zfp109
|
UTSW |
7 |
23,928,126 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R6092:Zfp109
|
UTSW |
7 |
23,928,978 (GRCm39) |
missense |
possibly damaging |
0.57 |
|
R6093:Zfp109
|
UTSW |
7 |
23,928,558 (GRCm39) |
missense |
probably benign |
0.03 |
|
R6334:Zfp109
|
UTSW |
7 |
23,928,308 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R6458:Zfp109
|
UTSW |
7 |
23,927,870 (GRCm39) |
missense |
probably benign |
0.01 |
|
R6856:Zfp109
|
UTSW |
7 |
23,928,823 (GRCm39) |
missense |
probably benign |
0.03 |
|
R6879:Zfp109
|
UTSW |
7 |
23,928,615 (GRCm39) |
missense |
probably benign |
0.02 |
|
R7069:Zfp109
|
UTSW |
7 |
23,928,785 (GRCm39) |
missense |
probably benign |
0.01 |
|
R7151:Zfp109
|
UTSW |
7 |
23,929,231 (GRCm39) |
missense |
probably benign |
0.01 |
|
R7822:Zfp109
|
UTSW |
7 |
23,928,570 (GRCm39) |
missense |
probably benign |
0.06 |
|
R8205:Zfp109
|
UTSW |
7 |
23,928,635 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8492:Zfp109
|
UTSW |
7 |
23,927,499 (GRCm39) |
missense |
possibly damaging |
0.79 |
|
Z1088:Zfp109
|
UTSW |
7 |
23,928,360 (GRCm39) |
missense |
probably benign |
0.08 |
|
Z1177:Zfp109
|
UTSW |
7 |
23,928,212 (GRCm39) |
missense |
probably damaging |
1.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- CTGAGCAATCCCAGACAGAAATTTC -3'
(R):5'- GACACACTTTCTCCAGCAATGC -3'
Sequencing Primer
(F):5'- TTCTGACTTTCAGTAATGCTAGAATG -3'
(R):5'- TTTCTCCAGCAATGCCACAC -3'
|
| Posted On |
2015-03-18 |
Incidental Mutation