Mutagenetix > Incidental Mutation

Incidental Mutation 'R3772:Tenm4'

273355

Institutional Source

Beutler Lab

Gene Symbol

Tenm4

Ensembl Gene

ENSMUSG00000048078

Gene Name

teneurin transmembrane protein 4

Synonyms

l7Rn3, Doc4, Ten-m4, ELM2, l(7)-3Rn, Odz4

MMRRC Submission

040748-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R3772 (G1)

Quality Score

165

Status

Validated

Chromosome

Chromosomal Location

95820453-96560300 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 96344087 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Tryptophan at position 227 (R227W)

Ref Sequence

ENSEMBL: ENSMUSP00000102784 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000107162] [ENSMUST00000107165] [ENSMUST00000107166]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000107162
AA Change: R272W

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000102780
Gene: ENSMUSG00000048078
AA Change: R272W

Domain	Start	End	E-Value	Type
Pfam:Ten_N	10	410	5.6e-195	PFAM
transmembrane domain	411	433	N/A	INTRINSIC
EGF_like	637	665	3.43e1	SMART
EGF	668	696	2.29e1	SMART
EGF	701	730	1.88e-1	SMART
EGF	733	762	1.13e1	SMART
EGF	767	797	2.39e1	SMART
EGF	800	828	4.32e-1	SMART
EGF	831	859	6.02e0	SMART
EGF	862	894	9.93e-1	SMART
low complexity region	900	914	N/A	INTRINSIC
Pfam:RHS_repeat	2327	2380	5.5e-7	PFAM
Pfam:Tox-GHH	2740	2818	5.2e-34	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000107165
AA Change: R297W

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000102783
Gene: ENSMUSG00000048078
AA Change: R297W

Domain	Start	End	E-Value	Type
Pfam:Ten_N	36	402	1.1e-171	PFAM
transmembrane domain	403	425	N/A	INTRINSIC
EGF_like	629	657	3.43e1	SMART
EGF	660	688	2.29e1	SMART
EGF	693	722	1.88e-1	SMART
EGF	725	754	1.13e1	SMART
EGF	759	789	2.39e1	SMART
EGF	792	820	4.32e-1	SMART
EGF	823	851	6.02e0	SMART
EGF	863	895	9.93e-1	SMART
low complexity region	901	915	N/A	INTRINSIC
Pfam:RHS_repeat	2335	2368	1.6e-7	PFAM
Pfam:Tox-GHH	2749	2826	1.8e-32	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000107166
AA Change: R227W

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000102784
Gene: ENSMUSG00000048078
AA Change: R227W

Domain	Start	End	E-Value	Type
Pfam:Ten_N	35	193	1.4e-83	PFAM
Pfam:Ten_N	187	365	5e-78	PFAM
transmembrane domain	366	388	N/A	INTRINSIC
EGF_like	592	620	3.43e1	SMART
EGF	623	651	2.29e1	SMART
EGF	656	685	1.88e-1	SMART
EGF	688	717	1.13e1	SMART
EGF	722	752	2.39e1	SMART
EGF	755	783	4.32e-1	SMART
EGF	786	814	6.02e0	SMART
EGF	826	858	9.93e-1	SMART
low complexity region	864	878	N/A	INTRINSIC
Pfam:RHS_repeat	2298	2351	3.8e-7	PFAM
Pfam:Tox-GHH	2711	2789	3.9e-34	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000131541

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.5%

Validation Efficiency

100% (77/77)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene plays a role in establishing proper neuronal connectivity during development. Defects in this gene have been associated with hereditary essential tremor-5. [provided by RefSeq, Oct 2016]
PHENOTYPE: Various ENU-induced alleles cause prenatal lethality associated with impaired mesoderm development and lead to pleiotropic phenotypes. The most severe alleles cause failure of gastrulation and somitogenesis while the least severe one allows survival to adulthood with runting of variable penetrance. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb11	T	A	2: 69,159,720 (GRCm39)		probably benign	Het
Adgrl1	A	G	8: 84,649,633 (GRCm39)	N97S	possibly damaging	Het
Aldh1a2	A	G	9: 71,160,202 (GRCm39)	D76G	probably damaging	Het
Aldh3a1	A	G	11: 61,105,431 (GRCm39)	E179G	possibly damaging	Het
Ap1g1	A	G	8: 110,564,418 (GRCm39)	D324G	probably damaging	Het
Arfgap2	A	G	2: 91,095,711 (GRCm39)	T12A	probably benign	Het
Aurka	A	G	2: 172,208,880 (GRCm39)	L85P	probably benign	Het
Birc6	T	A	17: 74,925,424 (GRCm39)		probably benign	Het
Bmp7	A	T	2: 172,712,015 (GRCm39)	I403N	probably damaging	Het
Carns1	A	G	19: 4,220,915 (GRCm39)		probably benign	Het
Ccdc88c	G	A	12: 100,932,359 (GRCm39)		probably benign	Het
Ccl2	C	T	11: 81,927,784 (GRCm39)	A76V	probably damaging	Het
Cd109	CATTTATTTATTTATTTATTTATTTATTTATTTAT	CATTTATTTATTTATTTATTTATTTATTTATTTATTTAT	9: 78,619,782 (GRCm39)		probably benign	Het
Clstn2	A	G	9: 97,464,615 (GRCm39)	I180T	probably damaging	Het
Col24a1	T	C	3: 145,251,041 (GRCm39)	L1680P	probably damaging	Het
Col4a6	C	A	X: 139,955,196 (GRCm39)	G1416C	probably damaging	Het
Ctnna2	T	G	6: 76,950,752 (GRCm39)	N573T	probably damaging	Het
Cts8	G	A	13: 61,398,715 (GRCm39)		probably benign	Het
Cxcl17	A	G	7: 25,099,754 (GRCm39)		probably benign	Het
Defb18	T	C	1: 18,306,845 (GRCm39)	H37R	possibly damaging	Het
Dis3l2	T	C	1: 86,782,130 (GRCm39)	I229T	probably benign	Het
Dysf	G	A	6: 84,129,333 (GRCm39)	S1474N	possibly damaging	Het
Elf1	T	C	14: 79,804,650 (GRCm39)	V105A	possibly damaging	Het
F13a1	T	C	13: 37,082,108 (GRCm39)	K532R	probably benign	Het
Fmn1	T	G	2: 113,412,463 (GRCm39)	S996A	probably damaging	Het
Focad	A	C	4: 88,254,398 (GRCm39)		probably benign	Het
Frmd4a	A	T	2: 4,595,433 (GRCm39)	E109D	probably damaging	Het
Frrs1	T	G	3: 116,672,036 (GRCm39)	S45A	possibly damaging	Het
Gm5422	T	A	10: 31,124,510 (GRCm39)		noncoding transcript	Het
Gm5866	T	C	5: 52,740,088 (GRCm39)		noncoding transcript	Het
Hmcn2	C	T	2: 31,250,908 (GRCm39)	T790M	probably damaging	Het
Iglon5	A	T	7: 43,130,037 (GRCm39)	Y42*	probably null	Het
Khdrbs2	C	T	1: 32,283,157 (GRCm39)	Q90*	probably null	Het
Krt74	T	C	15: 101,670,630 (GRCm39)		noncoding transcript	Het
Lamc2	T	A	1: 152,999,997 (GRCm39)	M1121L	probably benign	Het
Lrig1	A	G	6: 94,582,798 (GRCm39)	L1073P	probably benign	Het
Lrp5	G	A	19: 3,662,330 (GRCm39)	R173C	probably damaging	Het
Man2c1	C	A	9: 57,047,661 (GRCm39)		probably benign	Het
Megf10	G	A	18: 57,416,934 (GRCm39)	D768N	probably benign	Het
Mycbp2	T	C	14: 103,371,224 (GRCm39)	N4108S	possibly damaging	Het
Nid1	A	G	13: 13,651,003 (GRCm39)		probably benign	Het
Nnt	A	G	13: 119,533,488 (GRCm39)	V59A	probably damaging	Het
Nsd1	G	A	13: 55,394,486 (GRCm39)	V696I	probably benign	Het
Or1o4	T	C	17: 37,590,745 (GRCm39)	T189A	probably benign	Het
Pag1	G	A	3: 9,764,688 (GRCm39)	T155M	probably benign	Het
Pgam5	T	C	5: 110,413,459 (GRCm39)	H176R	probably damaging	Het
Pid1	T	C	1: 84,015,918 (GRCm39)	D149G	probably damaging	Het
Pkp3	G	A	7: 140,662,259 (GRCm39)	M1I	probably null	Het
Pld2	C	T	11: 70,434,949 (GRCm39)		probably benign	Het
Ptpro	T	A	6: 137,420,592 (GRCm39)	V1007D	probably damaging	Het
Ptprs	T	C	17: 56,735,978 (GRCm39)	T152A	possibly damaging	Het
Rab9b	T	A	X: 135,762,198 (GRCm39)	E67D	probably damaging	Het
Rin2	C	T	2: 145,702,366 (GRCm39)	T354I	probably benign	Het
Rnf214	T	C	9: 45,777,932 (GRCm39)	M625V	possibly damaging	Het
Rwdd2a	A	C	9: 86,456,214 (GRCm39)	N130T	possibly damaging	Het
Scn9a	T	C	2: 66,313,992 (GRCm39)	N1909D	probably benign	Het
Septin10	A	T	10: 59,012,709 (GRCm39)	M303K	probably damaging	Het
Sez6l2	A	G	7: 126,558,375 (GRCm39)	E339G	probably damaging	Het
Sf3b1	C	A	1: 55,039,150 (GRCm39)		probably benign	Het
Shisal1	A	T	15: 84,290,886 (GRCm39)	Y120*	probably null	Het
Ska3	C	T	14: 58,047,534 (GRCm39)	V334I	probably benign	Het
Srebf2	A	G	15: 82,066,309 (GRCm39)	K579R	probably benign	Het
St18	A	T	1: 6,914,553 (GRCm39)	K799I	probably damaging	Het
Strada	C	T	11: 106,055,648 (GRCm39)	R333Q	probably damaging	Het
Stradb	A	T	1: 59,024,544 (GRCm39)	I64L	probably benign	Het
Sun1	A	G	5: 139,224,575 (GRCm39)		probably benign	Het
Tecta	T	C	9: 42,242,292 (GRCm39)	T2094A	probably damaging	Het
Tnk2	A	G	16: 32,498,640 (GRCm39)	D651G	probably damaging	Het
Trim43c	A	T	9: 88,729,810 (GRCm39)	D417V	probably damaging	Het
Tsc22d4	T	C	5: 137,757,495 (GRCm39)	L374P	possibly damaging	Het
Ttn	T	C	2: 76,601,711 (GRCm39)	T16872A	probably benign	Het
Ubr4	T	C	4: 139,180,011 (GRCm39)	V262A	possibly damaging	Het
Vmn2r60	A	T	7: 41,765,980 (GRCm39)	N29I	probably benign	Het
Xrn2	T	C	2: 146,903,207 (GRCm39)	V765A	probably benign	Het
Zbed4	C	T	15: 88,664,990 (GRCm39)	P353S	probably benign	Het
Zfp251	A	G	15: 76,737,836 (GRCm39)	I414T	possibly damaging	Het
Zfp426	A	T	9: 20,384,413 (GRCm39)		probably null	Het
Zwilch	A	T	9: 64,063,316 (GRCm39)	F286I	probably benign	Het

Other mutations in Tenm4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00228:Tenm4	APN	7	96,517,216 (GRCm39)	missense	probably benign	0.00
IGL00468:Tenm4	APN	7	96,523,679 (GRCm39)	missense	probably damaging	0.98
IGL00519:Tenm4	APN	7	96,454,345 (GRCm39)	splice site	probably benign
IGL00979:Tenm4	APN	7	96,378,598 (GRCm39)	missense	probably damaging	0.96
IGL01401:Tenm4	APN	7	96,523,474 (GRCm39)	missense	probably damaging	1.00
IGL01459:Tenm4	APN	7	96,378,592 (GRCm39)	missense	probably damaging	1.00
IGL01519:Tenm4	APN	7	96,544,384 (GRCm39)	missense	probably damaging	1.00
IGL01545:Tenm4	APN	7	96,523,510 (GRCm39)	missense	probably benign	0.00
IGL01579:Tenm4	APN	7	96,512,709 (GRCm39)	missense	probably benign	0.00
IGL01587:Tenm4	APN	7	96,512,709 (GRCm39)	missense	probably benign	0.00
IGL01625:Tenm4	APN	7	96,534,565 (GRCm39)	missense	probably damaging	1.00
IGL01655:Tenm4	APN	7	96,202,931 (GRCm39)	missense	probably damaging	1.00
IGL01683:Tenm4	APN	7	96,534,611 (GRCm39)	missense	possibly damaging	0.84
IGL01728:Tenm4	APN	7	96,545,271 (GRCm39)	missense	probably damaging	1.00
IGL01732:Tenm4	APN	7	96,544,716 (GRCm39)	missense	probably damaging	1.00
IGL01924:Tenm4	APN	7	96,544,419 (GRCm39)	missense	probably damaging	1.00
IGL01966:Tenm4	APN	7	96,202,757 (GRCm39)	missense	probably damaging	1.00
IGL02177:Tenm4	APN	7	96,544,869 (GRCm39)	missense	probably benign	0.40
IGL02207:Tenm4	APN	7	96,523,323 (GRCm39)	missense	possibly damaging	0.85
IGL02269:Tenm4	APN	7	96,473,029 (GRCm39)	missense	probably damaging	1.00
IGL02274:Tenm4	APN	7	96,503,941 (GRCm39)	missense	probably damaging	1.00
IGL02375:Tenm4	APN	7	96,353,344 (GRCm39)	missense	possibly damaging	0.52
IGL02415:Tenm4	APN	7	96,523,281 (GRCm39)	missense	probably damaging	0.98
IGL02472:Tenm4	APN	7	96,423,383 (GRCm39)	unclassified	probably benign
IGL02656:Tenm4	APN	7	96,534,640 (GRCm39)	missense	probably damaging	1.00
IGL02678:Tenm4	APN	7	96,545,426 (GRCm39)	missense	probably damaging	1.00
IGL02829:Tenm4	APN	7	96,544,205 (GRCm39)	nonsense	probably null
IGL02863:Tenm4	APN	7	96,522,913 (GRCm39)	missense	probably damaging	1.00
IGL03145:Tenm4	APN	7	96,492,175 (GRCm39)	missense	probably damaging	0.98
IGL03153:Tenm4	APN	7	96,522,969 (GRCm39)	missense	probably damaging	1.00
principium	UTSW	7	96,446,688 (GRCm39)	missense	probably damaging	0.98
toccata	UTSW	7	96,552,196 (GRCm39)	critical splice donor site	probably null
P0026:Tenm4	UTSW	7	96,523,734 (GRCm39)	missense	probably damaging	1.00
R0097:Tenm4	UTSW	7	96,542,133 (GRCm39)	missense	probably damaging	1.00
R0097:Tenm4	UTSW	7	96,542,133 (GRCm39)	missense	probably damaging	1.00
R0140:Tenm4	UTSW	7	96,545,259 (GRCm39)	missense	possibly damaging	0.78
R0164:Tenm4	UTSW	7	96,378,547 (GRCm39)	splice site	probably benign
R0277:Tenm4	UTSW	7	96,344,157 (GRCm39)	missense	possibly damaging	0.54
R0323:Tenm4	UTSW	7	96,344,157 (GRCm39)	missense	possibly damaging	0.54
R0362:Tenm4	UTSW	7	96,421,242 (GRCm39)	nonsense	probably null
R0381:Tenm4	UTSW	7	96,555,088 (GRCm39)	missense	probably damaging	1.00
R0420:Tenm4	UTSW	7	96,522,973 (GRCm39)	missense	possibly damaging	0.85
R0426:Tenm4	UTSW	7	96,427,058 (GRCm39)	missense	probably damaging	1.00
R0513:Tenm4	UTSW	7	96,544,830 (GRCm39)	missense	probably benign	0.35
R0624:Tenm4	UTSW	7	96,423,227 (GRCm39)	missense	probably damaging	1.00
R0837:Tenm4	UTSW	7	96,545,482 (GRCm39)	splice site	probably benign
R1037:Tenm4	UTSW	7	96,446,688 (GRCm39)	missense	probably damaging	0.98
R1172:Tenm4	UTSW	7	96,497,251 (GRCm39)	missense	probably damaging	1.00
R1422:Tenm4	UTSW	7	96,199,258 (GRCm39)	missense	probably damaging	0.99
R1427:Tenm4	UTSW	7	96,492,255 (GRCm39)	missense	probably benign	0.42
R1462:Tenm4	UTSW	7	96,353,360 (GRCm39)	missense	probably damaging	1.00
R1462:Tenm4	UTSW	7	96,353,360 (GRCm39)	missense	probably damaging	1.00
R1597:Tenm4	UTSW	7	96,552,196 (GRCm39)	critical splice donor site	probably null
R1701:Tenm4	UTSW	7	96,552,096 (GRCm39)	missense	probably damaging	1.00
R1707:Tenm4	UTSW	7	96,537,892 (GRCm39)	missense	probably damaging	1.00
R1809:Tenm4	UTSW	7	96,522,987 (GRCm39)	missense	probably benign	0.17
R1812:Tenm4	UTSW	7	96,545,147 (GRCm39)	missense	probably damaging	1.00
R1895:Tenm4	UTSW	7	96,385,015 (GRCm39)	missense	probably damaging	1.00
R1933:Tenm4	UTSW	7	96,544,533 (GRCm39)	missense	probably damaging	1.00
R1946:Tenm4	UTSW	7	96,385,015 (GRCm39)	missense	probably damaging	1.00
R2108:Tenm4	UTSW	7	96,555,497 (GRCm39)	missense	probably damaging	1.00
R2151:Tenm4	UTSW	7	96,552,054 (GRCm39)	missense	probably damaging	1.00
R2247:Tenm4	UTSW	7	96,555,216 (GRCm39)	missense	probably benign	0.03
R2329:Tenm4	UTSW	7	96,545,069 (GRCm39)	missense	probably benign	0.00
R2893:Tenm4	UTSW	7	96,544,197 (GRCm39)	missense	probably damaging	1.00
R2990:Tenm4	UTSW	7	96,542,332 (GRCm39)	splice site	probably null
R3409:Tenm4	UTSW	7	96,544,367 (GRCm39)	missense	probably damaging	1.00
R3410:Tenm4	UTSW	7	96,501,737 (GRCm39)	missense	probably damaging	0.99
R3411:Tenm4	UTSW	7	96,501,737 (GRCm39)	missense	probably damaging	0.99
R3440:Tenm4	UTSW	7	96,202,723 (GRCm39)	missense	probably benign	0.00
R3441:Tenm4	UTSW	7	96,202,723 (GRCm39)	missense	probably benign	0.00
R3719:Tenm4	UTSW	7	96,512,770 (GRCm39)	missense	possibly damaging	0.92
R3773:Tenm4	UTSW	7	96,344,087 (GRCm39)	missense	probably damaging	1.00
R4093:Tenm4	UTSW	7	96,544,979 (GRCm39)	missense	probably damaging	1.00
R4439:Tenm4	UTSW	7	96,545,022 (GRCm39)	missense	probably benign	0.01
R4441:Tenm4	UTSW	7	96,545,022 (GRCm39)	missense	probably benign	0.01
R4510:Tenm4	UTSW	7	96,544,070 (GRCm39)	missense	probably benign
R4511:Tenm4	UTSW	7	96,544,070 (GRCm39)	missense	probably benign
R4543:Tenm4	UTSW	7	96,545,022 (GRCm39)	missense	probably benign	0.01
R4645:Tenm4	UTSW	7	96,544,949 (GRCm39)	missense	probably damaging	1.00
R4701:Tenm4	UTSW	7	96,544,556 (GRCm39)	missense	probably damaging	1.00
R4707:Tenm4	UTSW	7	96,423,253 (GRCm39)	missense	probably damaging	0.99
R4714:Tenm4	UTSW	7	96,544,131 (GRCm39)	missense	probably damaging	1.00
R4742:Tenm4	UTSW	7	96,446,691 (GRCm39)	missense	probably damaging	0.99
R4784:Tenm4	UTSW	7	96,423,253 (GRCm39)	missense	probably damaging	0.99
R4785:Tenm4	UTSW	7	96,423,253 (GRCm39)	missense	probably damaging	0.99
R4801:Tenm4	UTSW	7	96,555,452 (GRCm39)	missense	probably damaging	0.97
R4802:Tenm4	UTSW	7	96,555,452 (GRCm39)	missense	probably damaging	0.97
R4880:Tenm4	UTSW	7	96,555,025 (GRCm39)	splice site	probably null
R5036:Tenm4	UTSW	7	96,501,768 (GRCm39)	missense	probably damaging	1.00
R5036:Tenm4	UTSW	7	96,343,997 (GRCm39)	missense	probably damaging	1.00
R5050:Tenm4	UTSW	7	96,544,995 (GRCm39)	missense	probably damaging	1.00
R5103:Tenm4	UTSW	7	96,492,164 (GRCm39)	missense	probably damaging	1.00
R5106:Tenm4	UTSW	7	96,492,356 (GRCm39)	missense	probably damaging	0.99
R5118:Tenm4	UTSW	7	96,542,293 (GRCm39)	missense	probably damaging	1.00
R5272:Tenm4	UTSW	7	96,523,410 (GRCm39)	missense	probably damaging	0.98
R5282:Tenm4	UTSW	7	96,486,538 (GRCm39)	missense	possibly damaging	0.90
R5403:Tenm4	UTSW	7	96,538,034 (GRCm39)	missense	probably damaging	1.00
R5404:Tenm4	UTSW	7	96,543,887 (GRCm39)	missense	probably damaging	1.00
R5567:Tenm4	UTSW	7	96,545,416 (GRCm39)	nonsense	probably null
R5590:Tenm4	UTSW	7	96,446,608 (GRCm39)	missense	possibly damaging	0.93
R5590:Tenm4	UTSW	7	96,446,607 (GRCm39)	missense	possibly damaging	0.73
R5597:Tenm4	UTSW	7	96,202,724 (GRCm39)	missense	probably benign	0.00
R5782:Tenm4	UTSW	7	96,542,246 (GRCm39)	missense	probably benign	0.00
R5861:Tenm4	UTSW	7	96,492,424 (GRCm39)	intron	probably benign
R5890:Tenm4	UTSW	7	96,552,067 (GRCm39)	missense	probably damaging	1.00
R5930:Tenm4	UTSW	7	96,503,926 (GRCm39)	missense	probably damaging	1.00
R5940:Tenm4	UTSW	7	96,495,102 (GRCm39)	missense	probably damaging	1.00
R6012:Tenm4	UTSW	7	96,171,640 (GRCm39)	intron	probably benign
R6060:Tenm4	UTSW	7	96,522,918 (GRCm39)	missense	probably damaging	1.00
R6104:Tenm4	UTSW	7	96,486,496 (GRCm39)	missense	probably damaging	0.97
R6283:Tenm4	UTSW	7	96,523,701 (GRCm39)	missense	probably benign	0.33
R6333:Tenm4	UTSW	7	96,423,331 (GRCm39)	missense	probably damaging	1.00
R6522:Tenm4	UTSW	7	96,492,251 (GRCm39)	missense	possibly damaging	0.88
R6616:Tenm4	UTSW	7	96,202,703 (GRCm39)	missense	probably benign	0.01
R6746:Tenm4	UTSW	7	96,542,067 (GRCm39)	missense	probably damaging	1.00
R6751:Tenm4	UTSW	7	96,494,919 (GRCm39)	missense	possibly damaging	0.95
R6806:Tenm4	UTSW	7	96,461,166 (GRCm39)	missense	possibly damaging	0.95
R6807:Tenm4	UTSW	7	96,544,478 (GRCm39)	missense	probably damaging	1.00
R6807:Tenm4	UTSW	7	96,202,703 (GRCm39)	missense	probably benign	0.01
R6809:Tenm4	UTSW	7	96,202,703 (GRCm39)	missense	probably benign	0.01
R6810:Tenm4	UTSW	7	96,202,703 (GRCm39)	missense	probably benign	0.01
R6811:Tenm4	UTSW	7	96,202,703 (GRCm39)	missense	probably benign	0.01
R6853:Tenm4	UTSW	7	96,486,502 (GRCm39)	missense	possibly damaging	0.94
R6886:Tenm4	UTSW	7	96,446,599 (GRCm39)	missense	possibly damaging	0.85
R6920:Tenm4	UTSW	7	96,544,757 (GRCm39)	missense	probably damaging	1.00
R6937:Tenm4	UTSW	7	96,202,703 (GRCm39)	missense	probably benign	0.01
R6939:Tenm4	UTSW	7	96,202,703 (GRCm39)	missense	probably benign	0.01
R7011:Tenm4	UTSW	7	96,545,342 (GRCm39)	nonsense	probably null
R7033:Tenm4	UTSW	7	96,544,430 (GRCm39)	nonsense	probably null
R7040:Tenm4	UTSW	7	96,202,703 (GRCm39)	missense	probably benign	0.01
R7083:Tenm4	UTSW	7	96,544,556 (GRCm39)	missense	probably damaging	1.00
R7238:Tenm4	UTSW	7	96,202,703 (GRCm39)	missense	probably benign	0.01
R7239:Tenm4	UTSW	7	96,385,020 (GRCm39)	missense	possibly damaging	0.47
R7239:Tenm4	UTSW	7	96,202,703 (GRCm39)	missense	probably benign	0.01
R7337:Tenm4	UTSW	7	96,523,333 (GRCm39)	missense	probably benign	0.44
R7400:Tenm4	UTSW	7	96,344,010 (GRCm39)	missense	probably damaging	0.97
R7407:Tenm4	UTSW	7	96,423,194 (GRCm39)	missense	possibly damaging	0.89
R7449:Tenm4	UTSW	7	96,523,420 (GRCm39)	missense	possibly damaging	0.65
R7473:Tenm4	UTSW	7	96,423,353 (GRCm39)	missense	probably damaging	1.00
R7477:Tenm4	UTSW	7	96,495,015 (GRCm39)	missense	probably damaging	0.99
R7489:Tenm4	UTSW	7	96,486,521 (GRCm39)	missense	possibly damaging	0.90
R7498:Tenm4	UTSW	7	96,497,224 (GRCm39)	missense	probably damaging	1.00
R7562:Tenm4	UTSW	7	96,538,021 (GRCm39)	missense	probably damaging	1.00
R7615:Tenm4	UTSW	7	96,495,133 (GRCm39)	missense	probably damaging	1.00
R7624:Tenm4	UTSW	7	96,545,192 (GRCm39)	missense	possibly damaging	0.95
R7626:Tenm4	UTSW	7	96,542,221 (GRCm39)	missense	probably damaging	1.00
R7690:Tenm4	UTSW	7	96,512,740 (GRCm39)	missense	probably benign	0.00
R7692:Tenm4	UTSW	7	96,544,610 (GRCm39)	missense	probably damaging	1.00
R7748:Tenm4	UTSW	7	96,543,909 (GRCm39)	missense	probably damaging	1.00
R7763:Tenm4	UTSW	7	96,544,899 (GRCm39)	missense	probably benign	0.38
R7792:Tenm4	UTSW	7	96,423,221 (GRCm39)	missense	possibly damaging	0.54
R7855:Tenm4	UTSW	7	96,523,081 (GRCm39)	missense	probably damaging	1.00
R7868:Tenm4	UTSW	7	96,555,587 (GRCm39)	missense	possibly damaging	0.79
R7878:Tenm4	UTSW	7	96,501,564 (GRCm39)	missense	probably damaging	1.00
R7997:Tenm4	UTSW	7	96,523,512 (GRCm39)	missense	probably benign	0.44
R8017:Tenm4	UTSW	7	96,353,248 (GRCm39)	missense	probably damaging	1.00
R8019:Tenm4	UTSW	7	96,353,248 (GRCm39)	missense	probably damaging	1.00
R8054:Tenm4	UTSW	7	96,378,553 (GRCm39)	splice site	probably benign
R8061:Tenm4	UTSW	7	96,501,663 (GRCm39)	missense	probably damaging	1.00
R8108:Tenm4	UTSW	7	96,503,935 (GRCm39)	missense	probably benign	0.39
R8140:Tenm4	UTSW	7	96,544,383 (GRCm39)	missense	probably damaging	1.00
R8214:Tenm4	UTSW	7	96,544,614 (GRCm39)	missense	probably damaging	1.00
R8258:Tenm4	UTSW	7	96,517,198 (GRCm39)	missense	probably damaging	1.00
R8259:Tenm4	UTSW	7	96,517,198 (GRCm39)	missense	probably damaging	1.00
R8364:Tenm4	UTSW	7	96,421,313 (GRCm39)	critical splice donor site	probably null
R8542:Tenm4	UTSW	7	96,461,139 (GRCm39)	missense	probably damaging	0.99
R8669:Tenm4	UTSW	7	96,555,148 (GRCm39)	missense	probably benign
R8670:Tenm4	UTSW	7	96,555,148 (GRCm39)	missense	probably benign
R8683:Tenm4	UTSW	7	96,552,064 (GRCm39)	missense	probably damaging	0.99
R8691:Tenm4	UTSW	7	96,555,148 (GRCm39)	missense	probably benign
R8692:Tenm4	UTSW	7	96,555,148 (GRCm39)	missense	probably benign
R8714:Tenm4	UTSW	7	96,555,148 (GRCm39)	missense	probably benign
R8716:Tenm4	UTSW	7	96,555,148 (GRCm39)	missense	probably benign
R8735:Tenm4	UTSW	7	96,555,148 (GRCm39)	missense	probably benign
R8736:Tenm4	UTSW	7	96,555,148 (GRCm39)	missense	probably benign
R8737:Tenm4	UTSW	7	96,555,148 (GRCm39)	missense	probably benign
R8738:Tenm4	UTSW	7	96,555,148 (GRCm39)	missense	probably benign
R8738:Tenm4	UTSW	7	96,523,047 (GRCm39)	missense	probably damaging	1.00
R8739:Tenm4	UTSW	7	96,555,148 (GRCm39)	missense	probably benign
R8776:Tenm4	UTSW	7	96,544,239 (GRCm39)	missense	probably damaging	1.00
R8776-TAIL:Tenm4	UTSW	7	96,544,239 (GRCm39)	missense	probably damaging	1.00
R8777:Tenm4	UTSW	7	96,545,244 (GRCm39)	missense	probably damaging	1.00
R8777-TAIL:Tenm4	UTSW	7	96,545,244 (GRCm39)	missense	probably damaging	1.00
R8817:Tenm4	UTSW	7	96,523,335 (GRCm39)	missense	probably benign	0.01
R8851:Tenm4	UTSW	7	96,501,710 (GRCm39)	missense	probably damaging	1.00
R8913:Tenm4	UTSW	7	96,351,952 (GRCm39)	splice site	probably benign
R8977:Tenm4	UTSW	7	96,461,177 (GRCm39)	missense	probably damaging	1.00
R9100:Tenm4	UTSW	7	96,495,061 (GRCm39)	missense	probably damaging	1.00
R9136:Tenm4	UTSW	7	96,473,125 (GRCm39)	missense	possibly damaging	0.69
R9163:Tenm4	UTSW	7	96,473,080 (GRCm39)	missense	probably damaging	1.00
R9188:Tenm4	UTSW	7	96,421,234 (GRCm39)	missense	probably damaging	1.00
R9195:Tenm4	UTSW	7	96,542,126 (GRCm39)	missense	probably damaging	1.00
R9217:Tenm4	UTSW	7	96,534,646 (GRCm39)	missense	probably damaging	1.00
R9344:Tenm4	UTSW	7	96,545,352 (GRCm39)	missense	probably damaging	1.00
R9414:Tenm4	UTSW	7	96,545,367 (GRCm39)	missense	probably benign
R9466:Tenm4	UTSW	7	96,199,252 (GRCm39)	missense	possibly damaging	0.79
R9559:Tenm4	UTSW	7	96,473,056 (GRCm39)	missense	probably benign
R9626:Tenm4	UTSW	7	96,545,345 (GRCm39)	missense	probably damaging	1.00
R9673:Tenm4	UTSW	7	96,517,196 (GRCm39)	missense	probably damaging	1.00
R9676:Tenm4	UTSW	7	96,544,638 (GRCm39)	missense	probably damaging	1.00
R9678:Tenm4	UTSW	7	96,386,619 (GRCm39)	missense	possibly damaging	0.94
R9775:Tenm4	UTSW	7	96,555,761 (GRCm39)	missense	possibly damaging	0.92
R9790:Tenm4	UTSW	7	96,538,046 (GRCm39)	missense	probably damaging	1.00
R9791:Tenm4	UTSW	7	96,538,046 (GRCm39)	missense	probably damaging	1.00
R9803:Tenm4	UTSW	7	96,202,685 (GRCm39)	missense	probably damaging	1.00
X0021:Tenm4	UTSW	7	96,523,116 (GRCm39)	nonsense	probably null
X0026:Tenm4	UTSW	7	96,517,294 (GRCm39)	missense	probably damaging	0.98
X0066:Tenm4	UTSW	7	96,544,001 (GRCm39)	missense	probably damaging	1.00
X0066:Tenm4	UTSW	7	96,497,237 (GRCm39)	missense	probably damaging	1.00
Z1176:Tenm4	UTSW	7	96,555,121 (GRCm39)	missense	probably benign	0.00
Z1177:Tenm4	UTSW	7	96,512,792 (GRCm39)	missense	probably damaging	0.96

Predicted Primers

PCR Primer
(F):5'- TTGCTAGTACTGGACACACTC -3'
(R):5'- ATAGTGTCCAGCTGGTCTGC -3'

Sequencing Primer
(F):5'- AGTACTGGACACACTCATTCTC -3'
(R):5'- AGCTGGTCTGCCCTCTG -3'

Posted On

2015-03-25