Mutagenetix > Incidental Mutation

Incidental Mutation 'R3774:Dnajc15'

273520

Institutional Source

Beutler Lab

Gene Symbol

Dnajc15

Ensembl Gene

ENSMUSG00000022013

Gene Name

DnaJ heat shock protein family (Hsp40) member C15

Synonyms

Dnajd1, 1110003P16Rik

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R3774 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

78063657-78112357 bp(-) (GRCm39)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

A to T at 78094377 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000154390 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000022590] [ENSMUST00000226459]

AlphaFold

Q78YY6

Predicted Effect

probably benign
Transcript: ENSMUST00000022590

SMART Domains

Protein: ENSMUSP00000022590
Gene: ENSMUSG00000022013

Domain	Start	End	E-Value	Type
low complexity region	34	53	N/A	INTRINSIC
DnaJ	93	147	6.8e-7	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000226459

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.4%
20x: 95.3%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased mitochondrial activity that results in rapid metabolism in fasted mice or mice fed a high fat diet. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acadm	A	G	3: 153,638,734 (GRCm39)	V213A	probably benign	Het
Ccndbp1	A	G	2: 120,839,581 (GRCm39)	K26R	possibly damaging	Het
Chrna10	T	C	7: 101,763,535 (GRCm39)	T87A	probably benign	Het
Col27a1	T	A	4: 63,232,963 (GRCm39)	N360K	probably benign	Het
Crispld2	T	C	8: 120,756,005 (GRCm39)	S325P	probably damaging	Het
Dgkd	T	A	1: 87,864,022 (GRCm39)	I79N	probably damaging	Het
Dhdh	T	A	7: 45,131,362 (GRCm39)	D157V	probably benign	Het
Fbxo44	G	T	4: 148,241,051 (GRCm39)	F179L	probably damaging	Het
Gm5565	T	A	5: 146,095,419 (GRCm39)	E192V	probably benign	Het
Gpat4	G	A	8: 23,670,171 (GRCm39)	P286L	probably damaging	Het
Itgav	A	G	2: 83,622,308 (GRCm39)	E630G	probably damaging	Het
Iws1	T	C	18: 32,213,048 (GRCm39)	S159P	probably damaging	Het
Kif23	G	A	9: 61,832,274 (GRCm39)	S623L	probably benign	Het
Krt32	A	G	11: 99,978,947 (GRCm39)	C36R	probably benign	Het
Med12l	A	C	3: 59,155,363 (GRCm39)	Q1181P	probably damaging	Het
Megf10	G	A	18: 57,410,177 (GRCm39)	G653S	probably damaging	Het
Mon1a	A	G	9: 107,778,502 (GRCm39)	Y242C	probably damaging	Het
Msh6	G	T	17: 88,293,609 (GRCm39)	R788L	probably damaging	Het
Mttp	T	C	3: 137,820,024 (GRCm39)		probably null	Het
Mxi1	C	A	19: 53,360,160 (GRCm39)	A294E	probably benign	Het
Olfm5	T	A	7: 103,811,056 (GRCm39)	R27S	possibly damaging	Het
Or52n2b	T	A	7: 104,566,113 (GRCm39)	Y130F	probably benign	Het
Palmd	T	C	3: 116,721,312 (GRCm39)	E81G	probably damaging	Het
Pecr	A	G	1: 72,298,530 (GRCm39)	F297L	probably benign	Het
Phf11b	A	G	14: 59,563,506 (GRCm39)	L137S	probably benign	Het
Phlpp1	GAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC	GAGCAGCAGCAGCAGCAGCAGCAGCAGC	1: 106,320,921 (GRCm39)		probably benign	Het
Plcb4	A	G	2: 135,800,065 (GRCm39)	K472E	probably benign	Het
Pomgnt1	G	A	4: 116,011,325 (GRCm39)	R230H	probably damaging	Het
Pomt1	A	G	2: 32,134,262 (GRCm39)	H261R	possibly damaging	Het
Ppm1d	T	C	11: 85,227,993 (GRCm39)	I303T	probably damaging	Het
Ptprc	T	G	1: 137,992,511 (GRCm39)	Q1205H	probably damaging	Het
Rad23b	C	T	4: 55,382,589 (GRCm39)	T264I	possibly damaging	Het
Rfc1	T	C	5: 65,421,749 (GRCm39)	Y1050C	probably damaging	Het
Septin8	T	C	11: 53,428,406 (GRCm39)	V352A	probably damaging	Het
Slc25a13	T	A	6: 6,109,288 (GRCm39)	Q358L	probably damaging	Het
Ssh1	T	C	5: 114,104,783 (GRCm39)	D12G	probably damaging	Het
Tmem59l	A	G	8: 70,939,951 (GRCm39)	L6S	unknown	Het
Tnik	T	C	3: 28,692,568 (GRCm39)	Y820H	probably damaging	Het
Trpm3	T	C	19: 22,955,966 (GRCm39)	F1143L	possibly damaging	Het
Trpm3	T	A	19: 22,965,339 (GRCm39)	S1611R	probably benign	Het
Ttyh3	A	G	5: 140,634,489 (GRCm39)	F32L	probably damaging	Het
Unkl	T	A	17: 25,407,381 (GRCm39)		probably null	Het
Vwf	T	A	6: 125,626,062 (GRCm39)		probably null	Het
Wdr11	T	A	7: 129,233,417 (GRCm39)		probably null	Het
Yeats2	A	G	16: 19,969,245 (GRCm39)	D12G	probably damaging	Het

Other mutations in Dnajc15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02607:Dnajc15	APN	14	78,077,656 (GRCm39)	missense	probably damaging	1.00
R0321:Dnajc15	UTSW	14	78,112,273 (GRCm39)	missense	possibly damaging	0.90
R1574:Dnajc15	UTSW	14	78,063,854 (GRCm39)	missense	probably benign	0.00
R1574:Dnajc15	UTSW	14	78,063,854 (GRCm39)	missense	probably benign	0.00
R4397:Dnajc15	UTSW	14	78,112,234 (GRCm39)	splice site	probably null
R4747:Dnajc15	UTSW	14	78,081,896 (GRCm39)	missense	probably benign	0.13
R5704:Dnajc15	UTSW	14	78,063,898 (GRCm39)	missense	probably damaging	1.00
R7848:Dnajc15	UTSW	14	78,077,643 (GRCm39)	missense	probably damaging	0.99
R8179:Dnajc15	UTSW	14	78,090,393 (GRCm39)	missense
R8882:Dnajc15	UTSW	14	78,094,411 (GRCm39)	missense	probably damaging	1.00
R9260:Dnajc15	UTSW	14	78,081,839 (GRCm39)	missense	possibly damaging	0.86

Predicted Primers

PCR Primer
(F):5'- CTGGGCCTCACTTTTGAACTG -3'
(R):5'- TCGTCAGAGCAGCACTTGAAG -3'

Sequencing Primer
(F):5'- TTCAAGCTAGCTGGTCCAAG -3'
(R):5'- GAGCAGCACTTGAAGATAGTTTATTC -3'

Posted On

2015-03-25