Mutagenetix > Incidental Mutation

Incidental Mutation 'R3775:Pgr15l'

273573

Institutional Source

Beutler Lab

Gene Symbol

Pgr15l

Ensembl Gene

ENSMUSG00000031212

Gene Name

G protein-coupled receptor 15-like

Synonyms

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.016) question?

Stock #

R3775 (G1)

Quality Score

222

Status

Validated

Chromosome

Chromosomal Location

96116202-96125710 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 96120747 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Methionine at position 181 (R181M)

Ref Sequence

ENSEMBL: ENSMUSP00000033556 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000033556]

AlphaFold

Q80T54

Predicted Effect

probably damaging
Transcript: ENSMUST00000033556
AA Change: R181M

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000033556
Gene: ENSMUSG00000031212
AA Change: R181M

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	83	361	4.2e-14	PFAM
Pfam:7tm_1	89	346	2e-49	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 94.9%

Validation Efficiency

100% (43/43)

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ahnak	A	G	19: 8,986,387 (GRCm39)	E2557G	possibly damaging	Het
Arid1a	A	G	4: 133,414,075 (GRCm39)	S1248P	unknown	Het
C2cd3	T	C	7: 100,081,205 (GRCm39)	L1327S	probably damaging	Het
Ccnh	T	C	13: 85,354,243 (GRCm39)		probably benign	Het
Dcdc5	C	A	2: 106,202,738 (GRCm39)		noncoding transcript	Het
Eprs1	T	C	1: 185,105,205 (GRCm39)	F160S	probably damaging	Het
F9	A	G	X: 59,064,345 (GRCm39)	I190V	probably benign	Het
Fam185a	C	A	5: 21,660,804 (GRCm39)	A273D	probably damaging	Het
Fhod1	G	A	8: 106,058,270 (GRCm39)		probably benign	Het
Gpat4	G	A	8: 23,670,171 (GRCm39)	P286L	probably damaging	Het
Hipk2	T	C	6: 38,720,029 (GRCm39)	D534G	probably damaging	Het
Ints6l	T	C	X: 55,526,731 (GRCm39)	L220S	probably damaging	Het
Kat7	T	C	11: 95,182,357 (GRCm39)	T250A	probably benign	Het
Kif23	G	A	9: 61,832,274 (GRCm39)	S623L	probably benign	Het
Krt32	A	G	11: 99,978,947 (GRCm39)	C36R	probably benign	Het
Megf10	G	A	18: 57,410,177 (GRCm39)	G653S	probably damaging	Het
Mpp3	G	T	11: 101,914,193 (GRCm39)	S134*	probably null	Het
Msh6	G	T	17: 88,293,609 (GRCm39)	R788L	probably damaging	Het
Mxi1	C	A	19: 53,360,160 (GRCm39)	A294E	probably benign	Het
Naip5	T	C	13: 100,359,902 (GRCm39)	I445V	probably benign	Het
Naip5	T	C	13: 100,359,883 (GRCm39)	E451G	probably benign	Het
Nlrp1b	T	C	11: 71,047,126 (GRCm39)		probably benign	Het
Npy1r	T	C	8: 67,157,502 (GRCm39)	F271L	possibly damaging	Het
Nup160	G	T	2: 90,552,420 (GRCm39)	C1132F	probably benign	Het
Or13p3	C	T	4: 118,567,351 (GRCm39)	T249I	probably damaging	Het
Or1o2	T	A	17: 37,543,121 (GRCm39)	I47F	probably damaging	Het
Pcdhga5	A	G	18: 37,828,167 (GRCm39)	E205G	possibly damaging	Het
Pdgfc	A	G	3: 81,048,858 (GRCm39)	T89A	probably damaging	Het
Pecr	A	G	1: 72,298,530 (GRCm39)	F297L	probably benign	Het
Pomgnt1	G	A	4: 116,011,325 (GRCm39)	R230H	probably damaging	Het
Ppm1d	T	C	11: 85,227,993 (GRCm39)	I303T	probably damaging	Het
Ptprc	T	G	1: 137,992,511 (GRCm39)	Q1205H	probably damaging	Het
Rnf112	A	T	11: 61,341,011 (GRCm39)		probably benign	Het
Slc25a13	T	A	6: 6,109,288 (GRCm39)	Q358L	probably damaging	Het
Slc25a19	A	G	11: 115,506,285 (GRCm39)	Y303H	probably damaging	Het
Sympk	T	C	7: 18,769,880 (GRCm39)	F186L	probably damaging	Het
Tek	A	G	4: 94,692,549 (GRCm39)	D219G	probably benign	Het
Tmem59l	A	G	8: 70,939,951 (GRCm39)	L6S	unknown	Het
Tnik	T	C	3: 28,692,568 (GRCm39)	Y820H	probably damaging	Het
Tnrc6c	C	T	11: 117,614,355 (GRCm39)	R838W	probably damaging	Het
Vmn2r29	C	G	7: 7,243,011 (GRCm39)	D500H	probably damaging	Het

Other mutations in Pgr15l

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01398:Pgr15l	APN	X	96,121,785 (GRCm39)	missense	probably benign	0.13
X0028:Pgr15l	UTSW	X	96,121,445 (GRCm39)	missense	probably damaging	1.00
Z1177:Pgr15l	UTSW	X	96,121,838 (GRCm39)	missense	possibly damaging	0.95

Predicted Primers

PCR Primer
(F):5'- ACTCTTACAACTGGAGACCATTTAG -3'
(R):5'- TGTGAACTTCCTGGGAATCTC -3'

Sequencing Primer
(F):5'- CCGAAACTACTTTAATACATCTGGGC -3'
(R):5'- GAACTTCCTGGGAATCTCTAGAAAG -3'

Posted On

2015-03-25