Mutagenetix > Incidental Mutation

Incidental Mutation 'R3498:Vmn1r176'

273657

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r176

Ensembl Gene

ENSMUSG00000096859

Gene Name

vomeronasal 1 receptor 176

Synonyms

Gm5998

MMRRC Submission

040661-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R3498 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

23534237-23535151 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 23534667 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Isoleucine at position 162 (K162I)

Ref Sequence

ENSEMBL: ENSMUSP00000153879 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171073] [ENSMUST00000226319] [ENSMUST00000226767] [ENSMUST00000227129] [ENSMUST00000227661] [ENSMUST00000228280] [ENSMUST00000228793]

AlphaFold

G3UW31

Predicted Effect

probably benign
Transcript: ENSMUST00000171073
AA Change: K162I

PolyPhen 2 Score 0.121 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000132168
Gene: ENSMUSG00000096859
AA Change: K162I

Domain	Start	End	E-Value	Type
Pfam:7tm_1	5	288	5.4e-6	PFAM
Pfam:TAS2R	8	295	1.8e-13	PFAM
Pfam:V1R	41	295	1.7e-21	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000226319
AA Change: K162I

PolyPhen 2 Score 0.121 (Sensitivity: 0.93; Specificity: 0.86)

Predicted Effect

probably benign
Transcript: ENSMUST00000226767
AA Change: K162I

PolyPhen 2 Score 0.121 (Sensitivity: 0.93; Specificity: 0.86)

Predicted Effect

probably benign
Transcript: ENSMUST00000227129
AA Change: K162I

PolyPhen 2 Score 0.121 (Sensitivity: 0.93; Specificity: 0.86)

Predicted Effect

probably benign
Transcript: ENSMUST00000227661
AA Change: K162I

PolyPhen 2 Score 0.121 (Sensitivity: 0.93; Specificity: 0.86)

Predicted Effect

probably benign
Transcript: ENSMUST00000228280
AA Change: K162I

PolyPhen 2 Score 0.121 (Sensitivity: 0.93; Specificity: 0.86)

Predicted Effect

probably benign
Transcript: ENSMUST00000228793
AA Change: K162I

PolyPhen 2 Score 0.121 (Sensitivity: 0.93; Specificity: 0.86)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.6%
20x: 96.0%

Validation Efficiency

100% (43/43)

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A3galt2	T	C	4: 128,649,350 (GRCm39)	F6L	probably benign	Het
Aurkc	A	G	7: 7,003,029 (GRCm39)	I175V	probably damaging	Het
Azi2	A	G	9: 117,878,475 (GRCm39)	D105G	probably damaging	Het
Bcat1	A	G	6: 144,965,068 (GRCm39)	V45A	probably damaging	Het
Cass4	C	T	2: 172,274,478 (GRCm39)	P753L	probably damaging	Het
Cimip3	AC	A	17: 47,744,348 (GRCm39)		probably benign	Het
Ddx42	A	G	11: 106,122,019 (GRCm39)	E178G	possibly damaging	Het
Dmpk	T	C	7: 18,820,306 (GRCm39)	I101T	probably damaging	Het
Dnah17	C	T	11: 117,971,675 (GRCm39)		probably benign	Het
Fosb	C	T	7: 19,040,557 (GRCm39)	R161H	probably damaging	Het
Gm6729	T	C	10: 86,376,582 (GRCm39)		noncoding transcript	Het
Gnb1	T	A	4: 155,639,483 (GRCm39)	N237K	possibly damaging	Het
Gpr35	A	G	1: 92,911,113 (GRCm39)	Y275C	probably damaging	Het
Hmcn1	A	T	1: 150,480,853 (GRCm39)	I4441N	probably damaging	Het
Ighe	G	A	12: 113,234,994 (GRCm39)	Q389*	probably null	Het
Kcnj11	T	C	7: 45,749,026 (GRCm39)	D23G	probably damaging	Het
Lats2	G	T	14: 57,959,923 (GRCm39)	A191E	possibly damaging	Het
Lyplal1	A	G	1: 185,820,857 (GRCm39)	S197P	possibly damaging	Het
Map4	G	T	9: 109,864,280 (GRCm39)	V502L	probably benign	Het
Mgat5	A	G	1: 127,312,571 (GRCm39)	M237V	possibly damaging	Het
Mindy4	A	G	6: 55,193,510 (GRCm39)	R68G	probably benign	Het
Nell1	T	A	7: 49,907,927 (GRCm39)	V362E	possibly damaging	Het
Or5m8	T	A	2: 85,822,774 (GRCm39)	F204L	probably benign	Het
Or6c66b	T	A	10: 129,376,778 (GRCm39)	I124N	probably damaging	Het
P4ha2	T	C	11: 54,010,079 (GRCm39)	Y279H	probably benign	Het
Pcid2	A	G	8: 13,150,413 (GRCm39)	V13A	possibly damaging	Het
Polr2j	T	C	5: 136,151,624 (GRCm39)	I116T	probably benign	Het
Prdm9	A	G	17: 15,783,207 (GRCm39)		probably benign	Het
Prr5	T	A	15: 84,587,345 (GRCm39)	V365E	probably benign	Het
Ptprf	A	T	4: 118,082,127 (GRCm39)	I1037N	probably damaging	Het
Ranbp17	GCCTGGATACTGACC	GCC	11: 33,169,203 (GRCm39)		probably benign	Het
Sde2	T	A	1: 180,685,750 (GRCm39)	C101S	probably damaging	Het
Sec1	T	C	7: 45,328,663 (GRCm39)	H128R	probably damaging	Het
Serpinb6a	A	T	13: 34,102,764 (GRCm39)	M253K	probably damaging	Het
Slc1a4	A	T	11: 20,263,973 (GRCm39)	I248N	probably damaging	Het
Slc22a4	T	G	11: 53,882,879 (GRCm39)	K328N	probably benign	Het
Slc24a4	A	T	12: 102,200,951 (GRCm39)	K278N	probably benign	Het
Slc6a21	T	A	7: 44,930,266 (GRCm39)	W222R	probably damaging	Het
Slc7a2	A	G	8: 41,365,567 (GRCm39)	E466G	probably benign	Het
Sspo	A	G	6: 48,444,914 (GRCm39)	T2133A	possibly damaging	Het
Taco1	A	G	11: 105,963,364 (GRCm39)	M172V	probably benign	Het
Tmem127	T	A	2: 127,098,040 (GRCm39)	H36Q	probably benign	Het
Tmem229b-ps	C	T	10: 53,351,223 (GRCm39)		noncoding transcript	Het
Vac14	A	G	8: 111,397,722 (GRCm39)	D479G	probably benign	Het

Other mutations in Vmn1r176

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01100:Vmn1r176	APN	7	23,535,049 (GRCm39)	missense	probably benign	0.04
IGL03004:Vmn1r176	APN	7	23,534,702 (GRCm39)	missense	probably damaging	1.00
PIT4151001:Vmn1r176	UTSW	7	23,534,808 (GRCm39)	missense	probably damaging	1.00
R0195:Vmn1r176	UTSW	7	23,535,010 (GRCm39)	missense	probably benign	0.10
R1186:Vmn1r176	UTSW	7	23,535,051 (GRCm39)	missense	probably damaging	1.00
R1531:Vmn1r176	UTSW	7	23,534,536 (GRCm39)	missense	possibly damaging	0.95
R1680:Vmn1r176	UTSW	7	23,534,806 (GRCm39)	missense	probably damaging	0.99
R1770:Vmn1r176	UTSW	7	23,534,946 (GRCm39)	missense	probably benign	0.06
R1803:Vmn1r176	UTSW	7	23,534,609 (GRCm39)	missense	probably damaging	1.00
R1970:Vmn1r176	UTSW	7	23,534,373 (GRCm39)	missense	probably benign	0.01
R2092:Vmn1r176	UTSW	7	23,534,578 (GRCm39)	missense	probably damaging	1.00
R4832:Vmn1r176	UTSW	7	23,534,463 (GRCm39)	missense	possibly damaging	0.67
R5712:Vmn1r176	UTSW	7	23,534,925 (GRCm39)	missense	probably benign	0.00
R6965:Vmn1r176	UTSW	7	23,535,099 (GRCm39)	missense	possibly damaging	0.78
R7105:Vmn1r176	UTSW	7	23,534,748 (GRCm39)	nonsense	probably null
R7839:Vmn1r176	UTSW	7	23,534,394 (GRCm39)	missense	possibly damaging	0.91
R8262:Vmn1r176	UTSW	7	23,534,878 (GRCm39)	missense	probably benign	0.29
R8870:Vmn1r176	UTSW	7	23,534,480 (GRCm39)	missense	probably damaging	1.00
R9147:Vmn1r176	UTSW	7	23,534,785 (GRCm39)	missense	probably benign	0.12
R9432:Vmn1r176	UTSW	7	23,534,743 (GRCm39)	missense	probably damaging	1.00
Z1088:Vmn1r176	UTSW	7	23,534,598 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- GTCTCAGCATGAACTCTGAGC -3'
(R):5'- GTACAAACATGTGCTCCACC -3'

Sequencing Primer
(F):5'- AGCATGAACTCTGAGCTTCTG -3'
(R):5'- AAACATGTGCTCCACCTGCTC -3'

Posted On

2015-04-02