Mutagenetix > Incidental Mutation

Incidental Mutation 'R3765:Tns3'

274666

Institutional Source

Beutler Lab

Gene Symbol

Tns3

Ensembl Gene

ENSMUSG00000020422

Gene Name

tensin 3

Synonyms

F830010I22Rik, Tens1, TEM6

MMRRC Submission

040742-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.333) question?

Stock #

R3765 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

8381652-8614681 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 8401133 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Valine at position 1055 (A1055V)

Ref Sequence

ENSEMBL: ENSMUSP00000020695 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020695]

AlphaFold

Q5SSZ5

Predicted Effect

probably benign
Transcript: ENSMUST00000020695
AA Change: A1055V

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000020695
Gene: ENSMUSG00000020422
AA Change: A1055V

Domain	Start	End	E-Value	Type
SCOP:d1d5ra2	1	171	5e-28	SMART
PTEN_C2	173	300	1.15e-48	SMART
low complexity region	854	864	N/A	INTRINSIC
low complexity region	1102	1126	N/A	INTRINSIC
SH2	1165	1268	1.32e-18	SMART
PTB	1301	1438	3.14e-24	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000129074

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.5%
20x: 95.8%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a null allele exhibit one third postnatal lethality, reduced body weight, growth retardation, smaller digestive tracts with defects in villi and enterocyte differentiation, abnormal lung morphology, and thinner bones with decreased chondrocyte proliferation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Akap13	T	C	7: 75,258,585 (GRCm39)	V403A	probably benign	Het
Arid2	T	C	15: 96,268,595 (GRCm39)	S903P	probably benign	Het
Arl6ip6	T	A	2: 53,082,243 (GRCm39)	W37R	probably damaging	Het
Bag3	C	T	7: 128,141,995 (GRCm39)	T162I	probably benign	Het
C4b	G	A	17: 34,948,814 (GRCm39)	P1545S	probably damaging	Het
Ccdc185	T	A	1: 182,575,117 (GRCm39)	H524L	possibly damaging	Het
Cfap43	T	C	19: 47,824,014 (GRCm39)	N119S	probably benign	Het
Churc1	C	A	12: 76,820,057 (GRCm39)	S22*	probably null	Het
Crbn	T	C	6: 106,771,987 (GRCm39)	K106E	possibly damaging	Het
Dag1	C	T	9: 108,085,398 (GRCm39)	G581E	probably damaging	Het
Dpp4	T	A	2: 62,216,780 (GRCm39)	T92S	probably benign	Het
Fam135a	T	C	1: 24,094,958 (GRCm39)	T137A	possibly damaging	Het
Fcsk	A	G	8: 111,613,736 (GRCm39)	I775T	probably benign	Het
Fermt1	T	C	2: 132,748,622 (GRCm39)	D667G	possibly damaging	Het
Foxl2	T	A	9: 98,838,039 (GRCm39)	I109N	probably damaging	Het
Frk	A	G	10: 34,360,001 (GRCm39)	M1V	probably null	Het
Gstm2	A	G	3: 107,891,346 (GRCm39)	F124S	probably damaging	Het
Hmcn1	T	C	1: 150,620,776 (GRCm39)	S1145G	possibly damaging	Het
Ints10	A	G	8: 69,277,771 (GRCm39)	T682A	possibly damaging	Het
Jmy	T	C	13: 93,601,219 (GRCm39)	M396V	possibly damaging	Het
Ldb3	A	T	14: 34,300,639 (GRCm39)		probably null	Het
Mre11a	A	G	9: 14,721,143 (GRCm39)	N354S	probably benign	Het
Nbea	A	G	3: 55,912,970 (GRCm39)	V939A	probably damaging	Het
Nphs1	T	C	7: 30,170,635 (GRCm39)	S928P	probably damaging	Het
Or5b109	G	A	19: 13,211,795 (GRCm39)	M60I	probably damaging	Het
Or7a40	A	G	16: 16,491,179 (GRCm39)	V222A	probably benign	Het
Or8g34	T	A	9: 39,372,920 (GRCm39)	Y61*	probably null	Het
Or9e1	T	C	11: 58,732,120 (GRCm39)	F60S	probably damaging	Het
Pla2g12b	G	A	10: 59,257,323 (GRCm39)	V169M	probably damaging	Het
Polr1c	C	T	17: 46,558,850 (GRCm39)	V14M	probably damaging	Het
Prg4	G	A	1: 150,327,122 (GRCm39)	S898L	probably damaging	Het
Prmt6	C	A	3: 110,157,510 (GRCm39)	E260*	probably null	Het
Ptx4	A	G	17: 25,341,842 (GRCm39)	T106A	probably benign	Het
Rab3il1	A	G	19: 10,005,673 (GRCm39)	T87A	probably damaging	Het
Sbf2	A	G	7: 109,974,788 (GRCm39)	V783A	probably damaging	Het
Scn2a	A	C	2: 65,513,054 (GRCm39)	D209A	possibly damaging	Het
Setd2	T	C	9: 110,423,314 (GRCm39)	L345P	probably damaging	Het
Slc18b1	A	G	10: 23,674,647 (GRCm39)	D34G	probably damaging	Het
Slc9c1	A	T	16: 45,411,244 (GRCm39)	M934L	possibly damaging	Het
Slx4	A	G	16: 3,798,850 (GRCm39)	V1357A	probably damaging	Het
Spidr	T	C	16: 15,786,504 (GRCm39)	E413G	probably benign	Het
Taar1	A	G	10: 23,797,205 (GRCm39)	Y301C	probably damaging	Het
Tada2b	A	T	5: 36,633,761 (GRCm39)	D197E	probably benign	Het
Taf1c	G	T	8: 120,327,224 (GRCm39)	Y418*	probably null	Het
Tanc2	A	G	11: 105,805,796 (GRCm39)	D394G	probably damaging	Het
Tnpo3	T	C	6: 29,579,688 (GRCm39)	D235G	probably benign	Het
Wdfy3	A	G	5: 102,009,266 (GRCm39)	Y2767H	probably damaging	Het
Zfhx3	A	G	8: 109,519,394 (GRCm39)	N172S	probably damaging	Het
Zfp839	T	C	12: 110,821,597 (GRCm39)	V137A	probably benign	Het

Other mutations in Tns3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00163:Tns3	APN	11	8,401,066 (GRCm39)	missense	probably benign	0.42
IGL00822:Tns3	APN	11	8,393,976 (GRCm39)	missense	probably damaging	0.99
IGL01075:Tns3	APN	11	8,428,399 (GRCm39)	missense	probably benign	0.45
IGL01286:Tns3	APN	11	8,442,617 (GRCm39)	missense	probably benign	0.01
IGL01680:Tns3	APN	11	8,498,937 (GRCm39)	missense	probably damaging	1.00
IGL01687:Tns3	APN	11	8,442,798 (GRCm39)	missense	probably damaging	1.00
IGL01734:Tns3	APN	11	8,469,192 (GRCm39)	splice site	probably benign
IGL01844:Tns3	APN	11	8,387,177 (GRCm39)	missense	possibly damaging	0.58
IGL01984:Tns3	APN	11	8,498,992 (GRCm39)	nonsense	probably null
IGL02137:Tns3	APN	11	8,442,578 (GRCm39)	missense	possibly damaging	0.93
IGL02273:Tns3	APN	11	8,384,531 (GRCm39)	missense	probably damaging	1.00
IGL02623:Tns3	APN	11	8,387,141 (GRCm39)	missense	probably damaging	1.00
IGL02697:Tns3	APN	11	8,442,346 (GRCm39)	missense	probably benign	0.00
IGL02829:Tns3	APN	11	8,469,564 (GRCm39)	missense	probably damaging	1.00
ANU74:Tns3	UTSW	11	8,442,149 (GRCm39)	missense	probably benign	0.38
R0020:Tns3	UTSW	11	8,495,227 (GRCm39)	critical splice donor site	probably null
R0064:Tns3	UTSW	11	8,385,856 (GRCm39)	nonsense	probably null
R0064:Tns3	UTSW	11	8,385,856 (GRCm39)	nonsense	probably null
R0370:Tns3	UTSW	11	8,395,730 (GRCm39)	missense	possibly damaging	0.80
R0388:Tns3	UTSW	11	8,395,703 (GRCm39)	missense	probably benign	0.07
R0410:Tns3	UTSW	11	8,385,852 (GRCm39)	missense	probably benign	0.02
R0496:Tns3	UTSW	11	8,497,262 (GRCm39)	splice site	probably benign
R0562:Tns3	UTSW	11	8,443,262 (GRCm39)	missense	possibly damaging	0.93
R0626:Tns3	UTSW	11	8,443,121 (GRCm39)	missense	probably benign	0.04
R0736:Tns3	UTSW	11	8,469,474 (GRCm39)	missense	possibly damaging	0.94
R0893:Tns3	UTSW	11	8,443,302 (GRCm39)	missense	probably damaging	1.00
R1367:Tns3	UTSW	11	8,398,704 (GRCm39)	missense	probably benign	0.01
R1386:Tns3	UTSW	11	8,468,261 (GRCm39)	missense	probably benign	0.02
R1975:Tns3	UTSW	11	8,385,738 (GRCm39)	missense	probably benign	0.04
R2205:Tns3	UTSW	11	8,481,719 (GRCm39)	missense	probably damaging	1.00
R2319:Tns3	UTSW	11	8,491,200 (GRCm39)	missense	probably damaging	1.00
R2830:Tns3	UTSW	11	8,385,870 (GRCm39)	missense	probably damaging	1.00
R3720:Tns3	UTSW	11	8,442,999 (GRCm39)	missense	probably damaging	1.00
R3817:Tns3	UTSW	11	8,384,619 (GRCm39)	missense	probably damaging	1.00
R4058:Tns3	UTSW	11	8,442,275 (GRCm39)	missense	probably damaging	1.00
R4599:Tns3	UTSW	11	8,481,747 (GRCm39)	missense	probably damaging	1.00
R4631:Tns3	UTSW	11	8,401,119 (GRCm39)	missense	probably benign	0.30
R4731:Tns3	UTSW	11	8,400,986 (GRCm39)	missense	probably benign	0.28
R4732:Tns3	UTSW	11	8,400,986 (GRCm39)	missense	probably benign	0.28
R4733:Tns3	UTSW	11	8,400,986 (GRCm39)	missense	probably benign	0.28
R5472:Tns3	UTSW	11	8,401,092 (GRCm39)	missense	probably benign
R5749:Tns3	UTSW	11	8,401,177 (GRCm39)	missense	probably benign	0.01
R5807:Tns3	UTSW	11	8,443,211 (GRCm39)	missense	probably damaging	1.00
R5844:Tns3	UTSW	11	8,384,580 (GRCm39)	missense	probably damaging	1.00
R5942:Tns3	UTSW	11	8,385,860 (GRCm39)	missense	probably damaging	1.00
R5982:Tns3	UTSW	11	8,442,245 (GRCm39)	missense	probably damaging	0.99
R6025:Tns3	UTSW	11	8,442,578 (GRCm39)	missense	possibly damaging	0.93
R6266:Tns3	UTSW	11	8,442,987 (GRCm39)	missense	probably damaging	1.00
R6322:Tns3	UTSW	11	8,442,147 (GRCm39)	missense	probably benign	0.01
R6536:Tns3	UTSW	11	8,384,531 (GRCm39)	missense	probably damaging	1.00
R6577:Tns3	UTSW	11	8,499,058 (GRCm39)	missense	probably damaging	1.00
R6577:Tns3	UTSW	11	8,499,057 (GRCm39)	missense	probably damaging	1.00
R6864:Tns3	UTSW	11	8,443,196 (GRCm39)	missense	probably damaging	1.00
R6897:Tns3	UTSW	11	8,481,743 (GRCm39)	missense	probably damaging	1.00
R7108:Tns3	UTSW	11	8,387,251 (GRCm39)	missense	probably benign	0.00
R7443:Tns3	UTSW	11	8,401,442 (GRCm39)	missense	probably benign	0.01
R7459:Tns3	UTSW	11	8,442,793 (GRCm39)	missense	probably benign	0.16
R7474:Tns3	UTSW	11	8,480,894 (GRCm39)	missense	probably damaging	1.00
R7576:Tns3	UTSW	11	8,491,192 (GRCm39)	missense	possibly damaging	0.78
R7979:Tns3	UTSW	11	8,442,701 (GRCm39)	missense	probably benign	0.01
R8055:Tns3	UTSW	11	8,495,343 (GRCm39)	missense	probably damaging	1.00
R8057:Tns3	UTSW	11	8,442,773 (GRCm39)	missense	probably benign
R8077:Tns3	UTSW	11	8,395,667 (GRCm39)	missense	probably damaging	1.00
R8518:Tns3	UTSW	11	8,442,971 (GRCm39)	missense	probably damaging	0.96
R8523:Tns3	UTSW	11	8,398,779 (GRCm39)	missense	probably damaging	1.00
R8790:Tns3	UTSW	11	8,468,273 (GRCm39)	missense	probably damaging	0.99
R9228:Tns3	UTSW	11	8,400,094 (GRCm39)	missense	probably damaging	1.00
R9374:Tns3	UTSW	11	8,442,606 (GRCm39)	missense	probably damaging	1.00
R9476:Tns3	UTSW	11	8,395,702 (GRCm39)	missense	probably damaging	0.99
R9510:Tns3	UTSW	11	8,395,702 (GRCm39)	missense	probably damaging	0.99
R9594:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
R9595:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
R9596:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
R9624:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
R9629:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
T0975:Tns3	UTSW	11	8,401,146 (GRCm39)	missense	probably benign	0.00
T0975:Tns3	UTSW	11	8,499,100 (GRCm39)	start gained	probably benign
T0975:Tns3	UTSW	11	8,429,518 (GRCm39)	missense	probably benign
X0005:Tns3	UTSW	11	8,429,518 (GRCm39)	missense	probably benign
X0005:Tns3	UTSW	11	8,401,224 (GRCm39)	missense	probably benign	0.00
Z1177:Tns3	UTSW	11	8,401,014 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AAGTCTGGAAGGACATTCGG -3'
(R):5'- CCATGAGCTGTCCATGTCAGAG -3'

Sequencing Primer
(F):5'- ATGCTCATGGTGCTGCC -3'
(R):5'- TGTCCATGTCAGAGCCTCAG -3'

Posted On

2015-04-02