Mutagenetix > Incidental Mutation

Incidental Mutation 'R3766:Vmn2r6'

274694

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r6

Ensembl Gene

ENSMUSG00000090581

Gene Name

vomeronasal 2, receptor 6

Synonyms

EG667069, EG620718

MMRRC Submission

040743-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.198) question?

Stock #

R3766 (G1)

Quality Score

204

Status

Not validated

Chromosome

Chromosomal Location

64444916-64472855 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 64463929 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Leucine at position 302 (I302L)

Ref Sequence

ENSEMBL: ENSMUSP00000135148 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165012] [ENSMUST00000176481]

AlphaFold

H3BK29

Predicted Effect

probably benign
Transcript: ENSMUST00000165012
AA Change: I213L

PolyPhen 2 Score 0.128 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000131831
Gene: ENSMUSG00000090581
AA Change: I213L

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	416	1.4e-72	PFAM
Pfam:Peripla_BP_6	58	244	1.2e-10	PFAM
Pfam:NCD3G	458	511	1.8e-17	PFAM
Pfam:7tm_3	542	779	3.9e-76	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000176481
AA Change: I302L

PolyPhen 2 Score 0.193 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000135148
Gene: ENSMUSG00000090581
AA Change: I302L

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	88	505	9.8e-77	PFAM
Pfam:Peripla_BP_6	142	331	3.4e-10	PFAM
Pfam:NCD3G	547	600	5.4e-17	PFAM
Pfam:7tm_3	633	867	3.9e-47	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.4%

Validation Efficiency

98% (41/42)

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aacs	C	T	5: 125,583,326 (GRCm39)	T294M	probably damaging	Het
Brf2	G	T	8: 27,614,496 (GRCm39)	T230N	possibly damaging	Het
Ccne2	A	G	4: 11,199,293 (GRCm39)		probably benign	Het
Crbn	T	C	6: 106,771,987 (GRCm39)	K106E	possibly damaging	Het
Cttnbp2nl	G	A	3: 104,912,117 (GRCm39)	T589I	probably benign	Het
Dock10	A	G	1: 80,514,643 (GRCm39)	S1091P	probably damaging	Het
Fh1	A	G	1: 175,442,316 (GRCm39)	V178A	probably damaging	Het
Fndc1	A	G	17: 8,003,253 (GRCm39)	S111P	probably damaging	Het
Frk	A	G	10: 34,360,001 (GRCm39)	M1V	probably null	Het
Gm29394	C	T	15: 57,912,024 (GRCm39)		probably benign	Het
Herc2	A	G	7: 55,813,572 (GRCm39)	D2601G	probably damaging	Het
Hspa13	T	C	16: 75,561,974 (GRCm39)	D75G	probably benign	Het
Itgav	T	C	2: 83,632,229 (GRCm39)		probably null	Het
Kif6	A	T	17: 50,065,671 (GRCm39)		probably benign	Het
Lypla1	G	A	1: 4,911,201 (GRCm39)	R104Q	probably benign	Het
Map1b	T	C	13: 99,570,595 (GRCm39)	K709E	unknown	Het
Or52l1	A	T	7: 104,830,088 (GRCm39)	I159K	probably damaging	Het
Or56b1b	G	A	7: 108,164,402 (GRCm39)	P200L	probably benign	Het
Pcdhb16	A	T	18: 37,611,249 (GRCm39)	K70*	probably null	Het
Pex5l	T	G	3: 33,061,327 (GRCm39)	D174A	probably benign	Het
Plac8l1	A	T	18: 42,313,460 (GRCm39)	M94K	probably benign	Het
Plxna1	T	C	6: 89,311,757 (GRCm39)		probably benign	Het
Psg26	T	C	7: 18,208,996 (GRCm39)	T471A	probably benign	Het
Pus3	C	A	9: 35,477,968 (GRCm39)	T400K	probably benign	Het
Pxk	T	C	14: 8,136,863 (GRCm38)		probably benign	Het
Rapgef2	T	C	3: 78,996,057 (GRCm39)	T569A	probably benign	Het
Sall4	T	C	2: 168,597,964 (GRCm39)	Q292R	possibly damaging	Het
Slc18b1	A	G	10: 23,674,647 (GRCm39)	D34G	probably damaging	Het
Slc45a1	T	C	4: 150,722,517 (GRCm39)	R456G	probably damaging	Het
Sox13	T	C	1: 133,318,536 (GRCm39)	R81G	possibly damaging	Het
Spag9	A	G	11: 93,951,109 (GRCm39)		probably benign	Het
Ston1	C	T	17: 88,942,788 (GRCm39)	P65S	probably damaging	Het
Tada2b	A	T	5: 36,633,761 (GRCm39)	D197E	probably benign	Het
Tcim	T	A	8: 24,928,765 (GRCm39)	R50W	probably damaging	Het
Tnpo3	T	C	6: 29,579,688 (GRCm39)	D235G	probably benign	Het
Trim59	A	G	3: 68,944,137 (GRCm39)	V401A	probably benign	Het
Trpm3	A	C	19: 22,425,741 (GRCm39)	Q32P	probably benign	Het
Tubgcp5	T	A	7: 55,480,614 (GRCm39)	M1018K	probably damaging	Het
Ube2o	A	G	11: 116,437,689 (GRCm39)		probably benign	Het
Ugcg	C	T	4: 59,207,798 (GRCm39)	P46S	probably benign	Het
Uvrag	G	T	7: 98,537,350 (GRCm39)	S615*	probably null	Het
Vmn2r73	C	T	7: 85,521,198 (GRCm39)	V257I	probably benign	Het

Other mutations in Vmn2r6

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01547:Vmn2r6	APN	3	64,445,525 (GRCm39)	missense	probably damaging	1.00
IGL01968:Vmn2r6	APN	3	64,463,766 (GRCm39)	missense	possibly damaging	0.94
IGL02009:Vmn2r6	APN	3	64,445,323 (GRCm39)	missense	possibly damaging	0.61
IGL02039:Vmn2r6	APN	3	64,463,610 (GRCm39)	missense	probably damaging	1.00
IGL02652:Vmn2r6	APN	3	64,463,749 (GRCm39)	missense	probably benign	0.24
IGL02737:Vmn2r6	APN	3	64,463,911 (GRCm39)	missense	possibly damaging	0.55
IGL02808:Vmn2r6	APN	3	64,463,917 (GRCm39)	missense	probably damaging	1.00
IGL03066:Vmn2r6	APN	3	64,472,574 (GRCm39)	missense	probably damaging	0.99
IGL03331:Vmn2r6	APN	3	64,445,428 (GRCm39)	missense	probably damaging	1.00
BB010:Vmn2r6	UTSW	3	64,467,224 (GRCm39)	missense	probably benign	0.02
BB020:Vmn2r6	UTSW	3	64,467,224 (GRCm39)	missense	probably benign	0.02
R0010:Vmn2r6	UTSW	3	64,466,966 (GRCm39)	nonsense	probably null
R0206:Vmn2r6	UTSW	3	64,447,333 (GRCm39)	missense	probably benign
R0206:Vmn2r6	UTSW	3	64,447,333 (GRCm39)	missense	probably benign
R0208:Vmn2r6	UTSW	3	64,447,333 (GRCm39)	missense	probably benign
R0427:Vmn2r6	UTSW	3	64,467,008 (GRCm39)	missense	probably damaging	1.00
R0466:Vmn2r6	UTSW	3	64,463,723 (GRCm39)	missense	probably damaging	1.00
R1018:Vmn2r6	UTSW	3	64,464,261 (GRCm39)	missense	probably benign	0.00
R1104:Vmn2r6	UTSW	3	64,445,487 (GRCm39)	missense	possibly damaging	0.93
R1186:Vmn2r6	UTSW	3	64,472,488 (GRCm39)	missense	probably benign	0.01
R1245:Vmn2r6	UTSW	3	64,464,211 (GRCm39)	missense	possibly damaging	0.53
R1295:Vmn2r6	UTSW	3	64,445,694 (GRCm39)	missense	probably damaging	1.00
R1473:Vmn2r6	UTSW	3	64,445,579 (GRCm39)	nonsense	probably null
R1498:Vmn2r6	UTSW	3	64,463,890 (GRCm39)	missense	probably damaging	1.00
R1925:Vmn2r6	UTSW	3	64,463,698 (GRCm39)	missense	possibly damaging	0.87
R2044:Vmn2r6	UTSW	3	64,445,262 (GRCm39)	missense	probably damaging	0.96
R2069:Vmn2r6	UTSW	3	64,463,519 (GRCm39)	missense	possibly damaging	0.89
R2253:Vmn2r6	UTSW	3	64,467,139 (GRCm39)	missense	probably damaging	1.00
R2261:Vmn2r6	UTSW	3	64,464,090 (GRCm39)	missense	probably benign	0.24
R2262:Vmn2r6	UTSW	3	64,464,090 (GRCm39)	missense	probably benign	0.24
R2350:Vmn2r6	UTSW	3	64,463,773 (GRCm39)	missense	probably benign	0.01
R2680:Vmn2r6	UTSW	3	64,445,707 (GRCm39)	missense	possibly damaging	0.91
R2846:Vmn2r6	UTSW	3	64,464,211 (GRCm39)	missense	possibly damaging	0.53
R2860:Vmn2r6	UTSW	3	64,454,760 (GRCm39)	missense	probably benign	0.00
R2861:Vmn2r6	UTSW	3	64,454,760 (GRCm39)	missense	probably benign	0.00
R3870:Vmn2r6	UTSW	3	64,464,042 (GRCm39)	missense	probably damaging	0.96
R4018:Vmn2r6	UTSW	3	64,463,893 (GRCm39)	missense	probably benign	0.05
R4024:Vmn2r6	UTSW	3	64,445,671 (GRCm39)	missense	possibly damaging	0.73
R4026:Vmn2r6	UTSW	3	64,445,671 (GRCm39)	missense	possibly damaging	0.73
R4227:Vmn2r6	UTSW	3	64,445,369 (GRCm39)	missense	probably damaging	0.99
R4526:Vmn2r6	UTSW	3	64,445,145 (GRCm39)	missense	probably benign	0.32
R4570:Vmn2r6	UTSW	3	64,467,068 (GRCm39)	missense	probably benign	0.31
R4894:Vmn2r6	UTSW	3	64,454,829 (GRCm39)	missense	probably benign
R4934:Vmn2r6	UTSW	3	64,463,766 (GRCm39)	missense	probably damaging	0.99
R5057:Vmn2r6	UTSW	3	64,445,207 (GRCm39)	missense	probably damaging	1.00
R5059:Vmn2r6	UTSW	3	64,445,044 (GRCm39)	missense	possibly damaging	0.89
R5148:Vmn2r6	UTSW	3	64,464,015 (GRCm39)	missense	probably damaging	0.99
R5155:Vmn2r6	UTSW	3	64,445,935 (GRCm39)	missense	probably benign	0.44
R5179:Vmn2r6	UTSW	3	64,445,411 (GRCm39)	missense	probably benign	0.00
R5256:Vmn2r6	UTSW	3	64,464,263 (GRCm39)	missense	probably benign	0.33
R5861:Vmn2r6	UTSW	3	64,463,454 (GRCm39)	missense	probably benign	0.00
R5950:Vmn2r6	UTSW	3	64,472,652 (GRCm39)	missense	probably benign	0.05
R6081:Vmn2r6	UTSW	3	64,463,953 (GRCm39)	missense	probably benign	0.25
R6173:Vmn2r6	UTSW	3	64,467,176 (GRCm39)	missense	probably damaging	1.00
R6190:Vmn2r6	UTSW	3	64,445,424 (GRCm39)	missense	probably benign	0.04
R6240:Vmn2r6	UTSW	3	64,464,226 (GRCm39)	missense	probably damaging	1.00
R6433:Vmn2r6	UTSW	3	64,454,801 (GRCm39)	nonsense	probably null
R6645:Vmn2r6	UTSW	3	64,464,297 (GRCm39)	missense	probably damaging	1.00
R6791:Vmn2r6	UTSW	3	64,445,580 (GRCm39)	missense	probably damaging	1.00
R7265:Vmn2r6	UTSW	3	64,464,195 (GRCm39)	missense	probably benign	0.00
R7503:Vmn2r6	UTSW	3	64,447,372 (GRCm39)	nonsense	probably null
R7562:Vmn2r6	UTSW	3	64,463,941 (GRCm39)	missense	probably benign	0.00
R7584:Vmn2r6	UTSW	3	64,472,683 (GRCm39)	missense	probably benign	0.07
R7611:Vmn2r6	UTSW	3	64,472,563 (GRCm39)	missense	probably damaging	0.98
R7759:Vmn2r6	UTSW	3	64,463,991 (GRCm39)	missense	probably damaging	1.00
R7834:Vmn2r6	UTSW	3	64,445,443 (GRCm39)	missense	probably damaging	1.00
R7933:Vmn2r6	UTSW	3	64,467,224 (GRCm39)	missense	probably benign	0.02
R7982:Vmn2r6	UTSW	3	64,467,241 (GRCm39)	missense	probably damaging	1.00
R8024:Vmn2r6	UTSW	3	64,467,245 (GRCm39)	missense	probably benign	0.40
R8074:Vmn2r6	UTSW	3	64,455,064 (GRCm39)	intron	probably benign
R8169:Vmn2r6	UTSW	3	64,447,310 (GRCm39)	missense	probably benign	0.01
R8337:Vmn2r6	UTSW	3	64,463,526 (GRCm39)	nonsense	probably null
R8736:Vmn2r6	UTSW	3	64,467,221 (GRCm39)	missense	probably damaging	1.00
R8962:Vmn2r6	UTSW	3	64,463,576 (GRCm39)	missense	probably damaging	1.00
R9139:Vmn2r6	UTSW	3	64,464,277 (GRCm39)	missense	probably benign	0.12
R9206:Vmn2r6	UTSW	3	64,467,032 (GRCm39)	missense	probably damaging	0.97
R9295:Vmn2r6	UTSW	3	64,463,484 (GRCm39)	missense	probably benign	0.00
R9332:Vmn2r6	UTSW	3	64,454,671 (GRCm39)	missense	probably benign	0.01
R9616:Vmn2r6	UTSW	3	64,445,724 (GRCm39)	missense	probably damaging	1.00
R9663:Vmn2r6	UTSW	3	64,463,549 (GRCm39)	missense	possibly damaging	0.90
R9685:Vmn2r6	UTSW	3	64,464,081 (GRCm39)	missense	probably benign	0.19
X0020:Vmn2r6	UTSW	3	64,445,871 (GRCm39)	missense	probably benign
X0066:Vmn2r6	UTSW	3	64,454,799 (GRCm39)	missense	probably damaging	1.00
Z1176:Vmn2r6	UTSW	3	64,463,746 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- GGATCCTTGCTAGGGTGTAC -3'
(R):5'- AATTCCAGTCTGAGGCCATCG -3'

Sequencing Primer
(F):5'- CCTTGCTAGGGTGTACATCATAAAGG -3'
(R):5'- GGCCATCGTGAATTTAATTAGACAC -3'

Posted On

2015-04-02