Mutagenetix > Incidental Mutation

Incidental Mutation 'R3826:Or9g4b'

275382

Institutional Source

Beutler Lab

Gene Symbol

Or9g4b

Ensembl Gene

ENSMUSG00000033850

Gene Name

olfactory receptor family 9 subfamily G member 4B

Synonyms

GA_x6K02T2Q125-47264151-47265089, Olfr1015, MOR213-3

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.262) question?

Stock #

R3826 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

85615818-85616833 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to T at 85616559 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Stop codon at position 235 (R235*)

Ref Sequence

ENSEMBL: ENSMUSP00000148957 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047870] [ENSMUST00000215945]

AlphaFold

Q7TR94

Predicted Effect

probably null
Transcript: ENSMUST00000047870
AA Change: R235*

SMART Domains

Protein: ENSMUSP00000046301
Gene: ENSMUSG00000033850
AA Change: R235*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	4.5e-49	PFAM
Pfam:7tm_1	41	290	4.2e-19	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000214726

Predicted Effect

probably null
Transcript: ENSMUST00000215945
AA Change: R235*

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.3%
3x: 98.8%
10x: 97.7%
20x: 96.3%

Validation Efficiency

100% (56/56)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadacl2fm1	T	A	3: 59,843,896 (GRCm39)	S197T	possibly damaging	Het
Actl6b	T	C	5: 137,565,535 (GRCm39)	L377P	probably damaging	Het
Apc	C	A	18: 34,412,388 (GRCm39)	Q236K	possibly damaging	Het
Atn1	G	A	6: 124,723,182 (GRCm39)		probably benign	Het
Ccr3	A	G	9: 123,829,714 (GRCm39)	T350A	possibly damaging	Het
Cdc42bpg	T	C	19: 6,367,675 (GRCm39)	V1015A	probably damaging	Het
Chd2	A	G	7: 73,141,163 (GRCm39)	Y577H	possibly damaging	Het
Col1a2	A	G	6: 4,516,960 (GRCm39)		probably benign	Het
Col4a1	C	A	8: 11,259,650 (GRCm39)	G1341V	probably damaging	Het
Commd9	C	A	2: 101,727,486 (GRCm39)	N93K	probably benign	Het
Cplane2	C	T	4: 140,945,900 (GRCm39)	R148C	probably damaging	Het
Cx3cl1	A	T	8: 95,503,934 (GRCm39)		probably benign	Het
Dhx37	T	C	5: 125,508,677 (GRCm39)	K86R	probably benign	Het
Dlec1	T	C	9: 118,972,129 (GRCm39)		probably benign	Het
Dnah17	G	A	11: 117,931,984 (GRCm39)		probably benign	Het
Fam234a	T	C	17: 26,437,163 (GRCm39)	E172G	probably benign	Het
Ffar2	A	T	7: 30,519,510 (GRCm39)	I10N	possibly damaging	Het
Gas2	T	C	7: 51,586,367 (GRCm39)		probably null	Het
Gpat4	G	A	8: 23,670,171 (GRCm39)	P286L	probably damaging	Het
Grpel1	T	A	5: 36,626,827 (GRCm39)	N36K	probably benign	Het
Hgfac	C	A	5: 35,205,506 (GRCm39)	D595E	probably damaging	Het
Kcnj10	A	T	1: 172,197,616 (GRCm39)	S377C	probably damaging	Het
Kcnq2	C	T	2: 180,746,693 (GRCm39)	V369I	possibly damaging	Het
Kcnt1	T	C	2: 25,805,880 (GRCm39)		probably null	Het
Lpcat1	T	C	13: 73,637,212 (GRCm39)	I114T	possibly damaging	Het
Mast4	G	T	13: 102,875,319 (GRCm39)	H1350N	probably damaging	Het
Mcts1	T	C	X: 37,691,445 (GRCm39)		probably benign	Het
Muc21	A	G	17: 35,932,504 (GRCm39)		probably benign	Het
Myg1	G	C	15: 102,246,171 (GRCm39)	G349R	probably damaging	Het
Ncor2	T	C	5: 125,195,756 (GRCm39)		probably benign	Het
Or1o4	T	A	17: 37,591,140 (GRCm39)	Y57F	probably damaging	Het
Or5t17	T	A	2: 86,832,388 (GRCm39)	I25K	probably damaging	Het
Panx2	A	G	15: 88,952,664 (GRCm39)	D377G	probably damaging	Het
Pcdhb10	C	A	18: 37,545,470 (GRCm39)	T182N	probably damaging	Het
Pdha2	G	T	3: 140,916,889 (GRCm39)	F206L	possibly damaging	Het
Pgd	T	C	4: 149,250,461 (GRCm39)		probably benign	Het
Pgs1	C	T	11: 117,910,584 (GRCm39)		probably null	Het
Rere	T	C	4: 150,554,785 (GRCm39)	V161A	probably benign	Het
Rgs12	G	A	5: 35,123,359 (GRCm39)	V381M	possibly damaging	Het
Rrm2	G	T	12: 24,758,598 (GRCm39)	A47S	probably benign	Het
Rsph6a	T	C	7: 18,791,539 (GRCm39)	L236P	probably damaging	Het
Rtkn2	A	T	10: 67,833,456 (GRCm39)		probably null	Het
Rubcnl	C	T	14: 75,269,665 (GRCm39)	L108F	possibly damaging	Het
Sap18b	T	C	8: 96,552,185 (GRCm39)	F65S	probably damaging	Het
Scap	A	G	9: 110,210,365 (GRCm39)	M925V	probably benign	Het
Slc7a8	A	G	14: 54,975,029 (GRCm39)	I200T	probably damaging	Het
Srrm3	A	G	5: 135,886,068 (GRCm39)	D336G	probably damaging	Het
Stk11	T	C	10: 79,963,782 (GRCm39)		probably null	Het
Tiam2	C	G	17: 3,557,976 (GRCm39)		probably benign	Het
Tmem59l	A	G	8: 70,939,951 (GRCm39)	L6S	unknown	Het
Trio	T	A	15: 27,833,156 (GRCm39)	K75N	probably damaging	Het
Tspan18	T	C	2: 93,050,453 (GRCm39)	I57V	probably benign	Het
Ube3b	C	A	5: 114,538,012 (GRCm39)	Q368K	probably damaging	Het
Zfhx4	A	T	3: 5,466,269 (GRCm39)	K2142N	probably damaging	Het
Zfp37	T	C	4: 62,110,800 (GRCm39)	N88S	probably benign	Het
Zswim8	C	T	14: 20,761,157 (GRCm39)	R142*	probably null	Het

Other mutations in Or9g4b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00902:Or9g4b	APN	2	85,616,461 (GRCm39)	missense	probably benign	0.21
IGL01716:Or9g4b	APN	2	85,616,487 (GRCm39)	missense	probably damaging	0.97
IGL03196:Or9g4b	APN	2	85,616,365 (GRCm39)	missense	possibly damaging	0.63
IGL03374:Or9g4b	APN	2	85,616,053 (GRCm39)	missense	probably damaging	1.00
R0329:Or9g4b	UTSW	2	85,616,147 (GRCm39)	nonsense	probably null
R0330:Or9g4b	UTSW	2	85,616,147 (GRCm39)	nonsense	probably null
R0714:Or9g4b	UTSW	2	85,616,743 (GRCm39)	missense	probably damaging	1.00
R0965:Or9g4b	UTSW	2	85,616,643 (GRCm39)	missense	probably damaging	1.00
R1078:Or9g4b	UTSW	2	85,616,437 (GRCm39)	missense	possibly damaging	0.53
R5031:Or9g4b	UTSW	2	85,616,062 (GRCm39)	nonsense	probably null
R5239:Or9g4b	UTSW	2	85,616,002 (GRCm39)	missense	probably damaging	1.00
R6120:Or9g4b	UTSW	2	85,616,685 (GRCm39)	missense	probably damaging	1.00
R6177:Or9g4b	UTSW	2	85,616,004 (GRCm39)	missense	probably damaging	0.99
R6726:Or9g4b	UTSW	2	85,615,906 (GRCm39)	missense	possibly damaging	0.51
R6954:Or9g4b	UTSW	2	85,616,726 (GRCm39)	nonsense	probably null
R7766:Or9g4b	UTSW	2	85,616,002 (GRCm39)	missense	probably damaging	1.00
R8193:Or9g4b	UTSW	2	85,616,305 (GRCm39)	missense	probably benign	0.34
R8245:Or9g4b	UTSW	2	85,616,119 (GRCm39)	missense	probably benign	0.02
R8339:Or9g4b	UTSW	2	85,615,876 (GRCm39)	missense	probably damaging	0.98
R9272:Or9g4b	UTSW	2	85,616,088 (GRCm39)	missense	probably benign	0.09
Z1176:Or9g4b	UTSW	2	85,616,464 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CACTTTCAGGCTAAGGTTCTGTG -3'
(R):5'- GTCTGCATCAACTTCTTAAAGGC -3'

Sequencing Primer
(F):5'- TTGTACAGACACCCGTGT -3'
(R):5'- GCATCAACTTCTTAAAGGCTTCTTTG -3'

Posted On

2015-04-02