Mutagenetix > Incidental Mutation

Incidental Mutation 'R3875:Zfp60'

276789

Institutional Source

Beutler Lab

Gene Symbol

Zfp60

Ensembl Gene

ENSMUSG00000037640

Gene Name

zinc finger protein 60

Synonyms

Mfg-3, Mfg3, 6330516O17Rik

MMRRC Submission

068967-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R3875 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

27430834-27451114 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 27449006 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 558 (I558T)

Ref Sequence

ENSEMBL: ENSMUSP00000132607 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042641] [ENSMUST00000108336] [ENSMUST00000130997] [ENSMUST00000136373] [ENSMUST00000167955]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000042641
AA Change: I558T

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000036627
Gene: ENSMUSG00000037640
AA Change: I558T

Domain	Start	End	E-Value	Type
KRAB	14	75	8.48e-36	SMART
ZnF_C2H2	173	195	2.67e-1	SMART
ZnF_C2H2	201	223	2.09e-3	SMART
ZnF_C2H2	229	251	1.18e-2	SMART
ZnF_C2H2	257	279	2.24e-3	SMART
ZnF_C2H2	285	307	2.65e-5	SMART
ZnF_C2H2	313	335	5.5e-3	SMART
ZnF_C2H2	341	363	3.89e-3	SMART
ZnF_C2H2	369	391	6.32e-3	SMART
ZnF_C2H2	397	419	5.14e-3	SMART
ZnF_C2H2	425	447	7.9e-4	SMART
ZnF_C2H2	453	475	1.67e-2	SMART
ZnF_C2H2	481	503	4.4e-2	SMART
ZnF_C2H2	509	531	4.24e-4	SMART
ZnF_C2H2	537	559	5.21e-4	SMART
ZnF_C2H2	565	587	5.14e-3	SMART
ZnF_C2H2	593	615	3.89e-3	SMART
ZnF_C2H2	621	643	1.95e-3	SMART
ZnF_C2H2	649	671	6.67e-2	SMART
ZnF_C2H2	677	699	8.34e-3	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000108336
AA Change: I558T

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000103973
Gene: ENSMUSG00000037640
AA Change: I558T

Domain	Start	End	E-Value	Type
KRAB	14	75	8.48e-36	SMART
ZnF_C2H2	173	195	2.67e-1	SMART
ZnF_C2H2	201	223	2.09e-3	SMART
ZnF_C2H2	229	251	1.18e-2	SMART
ZnF_C2H2	257	279	2.24e-3	SMART
ZnF_C2H2	285	307	2.65e-5	SMART
ZnF_C2H2	313	335	5.5e-3	SMART
ZnF_C2H2	341	363	3.89e-3	SMART
ZnF_C2H2	369	391	6.32e-3	SMART
ZnF_C2H2	397	419	5.14e-3	SMART
ZnF_C2H2	425	447	7.9e-4	SMART
ZnF_C2H2	453	475	1.67e-2	SMART
ZnF_C2H2	481	503	4.4e-2	SMART
ZnF_C2H2	509	531	4.24e-4	SMART
ZnF_C2H2	537	559	5.21e-4	SMART
ZnF_C2H2	565	587	5.14e-3	SMART
ZnF_C2H2	593	615	3.89e-3	SMART
ZnF_C2H2	621	643	1.95e-3	SMART
ZnF_C2H2	649	671	6.67e-2	SMART
ZnF_C2H2	677	699	8.34e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000130997

SMART Domains

Protein: ENSMUSP00000118469
Gene: ENSMUSG00000037640

Domain	Start	End	E-Value	Type
KRAB	14	75	8.48e-36	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000136373

SMART Domains

Protein: ENSMUSP00000117049
Gene: ENSMUSG00000037640

Domain	Start	End	E-Value	Type
KRAB	14	55	3.2e-13	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000167955
AA Change: I558T

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000132607
Gene: ENSMUSG00000037640
AA Change: I558T

Domain	Start	End	E-Value	Type
KRAB	14	75	8.48e-36	SMART
ZnF_C2H2	173	195	2.67e-1	SMART
ZnF_C2H2	201	223	2.09e-3	SMART
ZnF_C2H2	229	251	1.18e-2	SMART
ZnF_C2H2	257	279	2.24e-3	SMART
ZnF_C2H2	285	307	2.65e-5	SMART
ZnF_C2H2	313	335	5.5e-3	SMART
ZnF_C2H2	341	363	3.89e-3	SMART
ZnF_C2H2	369	391	6.32e-3	SMART
ZnF_C2H2	397	419	5.14e-3	SMART
ZnF_C2H2	425	447	7.9e-4	SMART
ZnF_C2H2	453	475	1.67e-2	SMART
ZnF_C2H2	481	503	4.4e-2	SMART
ZnF_C2H2	509	531	4.24e-4	SMART
ZnF_C2H2	537	559	5.21e-4	SMART
ZnF_C2H2	565	587	5.14e-3	SMART
ZnF_C2H2	593	615	3.89e-3	SMART
ZnF_C2H2	621	643	1.95e-3	SMART
ZnF_C2H2	649	671	6.67e-2	SMART
ZnF_C2H2	677	699	8.34e-3	SMART

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.6%
20x: 96.1%

Validation Efficiency

100% (50/50)

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
6820408C15Rik	T	C	2: 152,276,000 (GRCm39)	S72P	probably benign	Het
Abca5	T	A	11: 110,201,059 (GRCm39)	Y447F	probably damaging	Het
Adgrg2	A	G	X: 159,261,992 (GRCm39)	S337G	probably benign	Het
Akap12	G	A	10: 4,307,590 (GRCm39)	V1467I	probably benign	Het
Baz2a	T	A	10: 127,959,979 (GRCm39)	M1419K	probably damaging	Het
Cacna1g	G	A	11: 94,328,749 (GRCm39)	T1033I	probably damaging	Het
Car12	C	A	9: 66,624,834 (GRCm39)		probably benign	Het
Cpne1	G	A	2: 155,918,202 (GRCm39)	H352Y	probably damaging	Het
D630039A03Rik	T	A	4: 57,910,606 (GRCm39)	T69S	probably benign	Het
Dhodh	T	C	8: 110,321,592 (GRCm39)	D310G	probably null	Het
Dnah2	A	G	11: 69,320,174 (GRCm39)	I3965T	probably damaging	Het
Drd5	T	G	5: 38,477,157 (GRCm39)	V50G	possibly damaging	Het
Dst	C	A	1: 34,210,328 (GRCm39)	H934Q	probably damaging	Het
Eif1ad10	T	C	12: 88,216,476 (GRCm39)	D132G	unknown	Het
Far2	G	A	6: 148,052,089 (GRCm39)	E123K	probably benign	Het
Fbxo43	A	T	15: 36,162,249 (GRCm39)	F319L	probably benign	Het
Flii	T	C	11: 60,611,318 (GRCm39)	S418G	probably benign	Het
Glyctk	T	A	9: 106,034,820 (GRCm39)	Y82F	probably damaging	Het
Gm11353	G	T	13: 26,676,651 (GRCm39)		noncoding transcript	Het
Gm6505	T	A	3: 28,819,286 (GRCm39)		noncoding transcript	Het
Gpr151	A	G	18: 42,711,661 (GRCm39)	V339A	probably benign	Het
H2-T22	T	C	17: 36,351,195 (GRCm39)	I296V	probably benign	Het
Igfn1	T	C	1: 135,882,352 (GRCm39)	N2831S	probably damaging	Het
Igkv13-85	A	G	6: 68,907,484 (GRCm39)	V39A	probably damaging	Het
Irx5	A	G	8: 93,086,793 (GRCm39)	T242A	probably benign	Het
Krt26	T	C	11: 99,225,570 (GRCm39)	K304E	probably damaging	Het
Mcoln1	A	G	8: 3,558,355 (GRCm39)	D203G	probably benign	Het
Mlph	T	C	1: 90,855,844 (GRCm39)	C57R	probably damaging	Het
Myh13	C	A	11: 67,249,020 (GRCm39)	H1275Q	probably benign	Het
Nbeal1	T	A	1: 60,233,758 (GRCm39)		probably benign	Het
Orc5	T	A	5: 22,742,564 (GRCm39)	M115L	probably benign	Het
Pcdha6	T	A	18: 37,101,119 (GRCm39)	I104N	probably damaging	Het
Plcb4	T	C	2: 135,844,552 (GRCm39)	S157P	probably damaging	Het
Polq	A	G	16: 36,894,389 (GRCm39)	D1787G	probably damaging	Het
Prkar2b	T	G	12: 32,015,122 (GRCm39)	I142L	probably benign	Het
Ptprq	A	G	10: 107,520,965 (GRCm39)	S736P	possibly damaging	Het
Qrich2	A	T	11: 116,336,477 (GRCm39)	V2046D	probably damaging	Het
Rad21	A	T	15: 51,833,361 (GRCm39)	F373I	probably damaging	Het
Rcvrn	A	G	11: 67,590,880 (GRCm39)	I155V	probably benign	Het
Rsrc2	C	T	5: 123,874,691 (GRCm39)		probably benign	Het
Sgsh	A	G	11: 119,241,773 (GRCm39)	L111P	probably damaging	Het
Ssc5d	A	T	7: 4,930,261 (GRCm39)	D114V	probably damaging	Het
St6gal2	C	A	17: 55,789,698 (GRCm39)	P244Q	probably benign	Het
Tarbp1	A	G	8: 127,165,538 (GRCm39)		probably benign	Het
Tmem221	T	C	8: 72,008,399 (GRCm39)		probably null	Het
Trh	A	T	6: 92,220,679 (GRCm39)	V61E	possibly damaging	Het
Trim32	A	T	4: 65,531,703 (GRCm39)	I87F	possibly damaging	Het
Tti2	A	G	8: 31,641,175 (GRCm39)	K100E	probably benign	Het
Usp34	C	T	11: 23,439,033 (GRCm39)	P3532S	possibly damaging	Het
Vps13d	A	G	4: 144,917,114 (GRCm39)	Y17H	probably damaging	Het
Zfp106	T	C	2: 120,365,094 (GRCm39)	K438E	probably benign	Het
Zzef1	A	C	11: 72,779,866 (GRCm39)	I1880L	probably benign	Het

Other mutations in Zfp60

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03353:Zfp60	APN	7	27,447,759 (GRCm39)	nonsense	probably null
PIT4494001:Zfp60	UTSW	7	27,448,126 (GRCm39)	missense	probably damaging	1.00
R1281:Zfp60	UTSW	7	27,437,852 (GRCm39)	missense	probably damaging	1.00
R1643:Zfp60	UTSW	7	27,436,400 (GRCm39)	missense	probably damaging	0.98
R1883:Zfp60	UTSW	7	27,449,435 (GRCm39)	missense	probably benign	0.00
R1930:Zfp60	UTSW	7	27,436,382 (GRCm39)	start codon destroyed	probably null	1.00
R3623:Zfp60	UTSW	7	27,448,753 (GRCm39)	missense	probably benign	0.01
R3624:Zfp60	UTSW	7	27,448,753 (GRCm39)	missense	probably benign	0.01
R5296:Zfp60	UTSW	7	27,437,955 (GRCm39)	intron	probably benign
R5491:Zfp60	UTSW	7	27,447,940 (GRCm39)	splice site	probably null
R5724:Zfp60	UTSW	7	27,447,758 (GRCm39)	missense	probably benign
R6134:Zfp60	UTSW	7	27,449,323 (GRCm39)	missense	probably benign	0.01
R6312:Zfp60	UTSW	7	27,448,201 (GRCm39)	missense	probably damaging	0.99
R6477:Zfp60	UTSW	7	27,449,228 (GRCm39)	missense	probably benign	0.00
R6649:Zfp60	UTSW	7	27,448,151 (GRCm39)	missense	probably benign	0.00
R6653:Zfp60	UTSW	7	27,448,151 (GRCm39)	missense	probably benign	0.00
R7041:Zfp60	UTSW	7	27,448,451 (GRCm39)	missense	probably benign	0.05
R7166:Zfp60	UTSW	7	27,448,937 (GRCm39)	missense	possibly damaging	0.82
R7185:Zfp60	UTSW	7	27,437,830 (GRCm39)	missense	probably damaging	1.00
R7334:Zfp60	UTSW	7	27,448,444 (GRCm39)	missense	probably damaging	1.00
R7560:Zfp60	UTSW	7	27,448,696 (GRCm39)	missense	probably damaging	1.00
R7560:Zfp60	UTSW	7	27,448,561 (GRCm39)	missense	probably damaging	0.99
R7561:Zfp60	UTSW	7	27,447,955 (GRCm39)	missense	probably damaging	1.00
R7673:Zfp60	UTSW	7	27,447,740 (GRCm39)	missense	probably benign	0.00
R7961:Zfp60	UTSW	7	27,447,881 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- ACTGGAAAGAAACCCTTTGAGT -3'
(R):5'- CTTCTCACCAGTGTGGATCCTTT -3'

Sequencing Primer
(F):5'- AACCCTTTGAGTGTAAGGACTG -3'
(R):5'- ACATTCCTTGCACTGAAATGGC -3'

Posted On

2015-04-06