Mutagenetix > Incidental Mutation

Incidental Mutation 'IGL02174:Trmt10b'

283018

Institutional Source

Australian Phenomics Network (link to record)

Gene Symbol

Trmt10b

Ensembl Gene

ENSMUSG00000035601

Gene Name

tRNA methyltransferase 10B

Synonyms

2610042J10Rik, Rg9mtd3

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.268) question?

Stock #

IGL02174

Quality Score

Status

Chromosome

Chromosomal Location

45297127-45316131 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 45308508 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 223 (I223F)

Ref Sequence

ENSEMBL: ENSMUSP00000041052 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044673] [ENSMUST00000107800] [ENSMUST00000144781]

AlphaFold

Q9D075

Predicted Effect

possibly damaging
Transcript: ENSMUST00000044673
AA Change: I223F

PolyPhen 2 Score 0.878 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000041052
Gene: ENSMUSG00000035601
AA Change: I223F

Domain	Start	End	E-Value	Type
low complexity region	76	95	N/A	INTRINSIC
low complexity region	110	122	N/A	INTRINSIC
Pfam:tRNA_m1G_MT	135	308	2.6e-46	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000107800
AA Change: I221F

PolyPhen 2 Score 0.156 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000103429
Gene: ENSMUSG00000035601
AA Change: I221F

Domain	Start	End	E-Value	Type
low complexity region	76	95	N/A	INTRINSIC
low complexity region	108	120	N/A	INTRINSIC
Pfam:tRNA_m1G_MT	133	306	1.6e-46	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000126972

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141659

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142785

Predicted Effect

probably benign
Transcript: ENSMUST00000144781

SMART Domains

Protein: ENSMUSP00000114832
Gene: ENSMUSG00000035601

Domain	Start	End	E-Value	Type
low complexity region	76	95	N/A	INTRINSIC
low complexity region	110	122	N/A	INTRINSIC

Coding Region Coverage

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc4	T	C	14: 118,738,154 (GRCm39)	I1199V	probably damaging	Het
Adgrv1	G	A	13: 81,575,783 (GRCm39)	T4861I	probably benign	Het
Ampd2	T	C	3: 107,987,601 (GRCm39)	D108G	probably damaging	Het
Azin1	A	G	15: 38,493,730 (GRCm39)	F298L	probably benign	Het
Bag1	T	A	4: 40,941,555 (GRCm39)	E123D	possibly damaging	Het
Cabs1	A	G	5: 88,128,339 (GRCm39)	E330G	probably benign	Het
Ccdc33	T	C	9: 57,940,938 (GRCm39)	K435E	probably benign	Het
Clip2	T	G	5: 134,523,118 (GRCm39)	K943T	probably damaging	Het
Cmya5	T	C	13: 93,185,415 (GRCm39)	I3527V	possibly damaging	Het
Cyp2w1	C	T	5: 139,341,384 (GRCm39)	R244C	probably benign	Het
Dcaf13	C	A	15: 39,001,544 (GRCm39)	A284E	probably damaging	Het
Ddx31	T	A	2: 28,749,041 (GRCm39)	L279Q	probably damaging	Het
Dgki	A	G	6: 37,009,856 (GRCm39)	L497P	probably damaging	Het
Dnah12	A	T	14: 26,428,072 (GRCm39)	I233F	probably benign	Het
Dpp6	G	T	5: 27,926,085 (GRCm39)	G720*	probably null	Het
Emc1	T	C	4: 139,098,979 (GRCm39)	Y821H	possibly damaging	Het
Esr1	C	T	10: 4,948,003 (GRCm39)	R519W	probably damaging	Het
Gamt	A	T	10: 80,094,230 (GRCm39)	V227E	possibly damaging	Het
Habp2	A	G	19: 56,300,169 (GRCm39)	Q206R	probably damaging	Het
Ihh	A	G	1: 74,990,105 (GRCm39)	I90T	probably damaging	Het
Kif20b	T	C	19: 34,911,858 (GRCm39)		probably benign	Het
Lcp2	T	C	11: 34,000,966 (GRCm39)		probably benign	Het
Lyrm2	A	G	4: 32,800,649 (GRCm39)	I29V	probably benign	Het
Macf1	T	C	4: 123,385,587 (GRCm39)	D1168G	probably damaging	Het
Mocos	T	C	18: 24,828,953 (GRCm39)	S753P	probably benign	Het
Myo3a	A	G	2: 22,337,204 (GRCm39)	E367G	probably benign	Het
Ndor1	G	A	2: 25,139,206 (GRCm39)	A255V	possibly damaging	Het
Nfat5	C	T	8: 108,065,683 (GRCm39)	P148S	probably damaging	Het
Or4c121	A	T	2: 89,023,712 (GRCm39)	F222Y	probably benign	Het
Or5al7	T	A	2: 85,992,442 (GRCm39)	M284L	possibly damaging	Het
Or6z7	T	C	7: 6,483,438 (GRCm39)	Y239C	probably benign	Het
Or8b41	A	T	9: 38,055,081 (GRCm39)	T217S	possibly damaging	Het
Pappa2	C	T	1: 158,589,188 (GRCm39)	C1679Y	probably damaging	Het
Pcdhb2	T	A	18: 37,429,551 (GRCm39)	I508N	probably damaging	Het
Pkd2l1	G	A	19: 44,145,707 (GRCm39)	T172I	probably benign	Het
Pld5	A	G	1: 176,102,310 (GRCm39)	V44A	possibly damaging	Het
Prss44	T	C	9: 110,646,199 (GRCm39)	W309R	probably damaging	Het
Rc3h2	A	T	2: 37,301,237 (GRCm39)	N88K	probably benign	Het
Rspry1	A	G	8: 95,359,768 (GRCm39)	I102M	possibly damaging	Het
Stab2	A	G	10: 86,695,606 (GRCm39)		probably null	Het
Tbx3	C	A	5: 119,813,649 (GRCm39)	Y228*	probably null	Het
Tcf15	G	T	2: 151,986,065 (GRCm39)		probably benign	Het
Tmem41b	A	G	7: 109,578,003 (GRCm39)	S94P	possibly damaging	Het
Tyrp1	G	T	4: 80,763,063 (GRCm39)	G317*	probably null	Het
Urod	T	A	4: 116,847,479 (GRCm39)		probably benign	Het
Vwf	G	T	6: 125,532,358 (GRCm39)	R52L	probably damaging	Het
Wnk2	C	A	13: 49,210,643 (GRCm39)	Q232H	probably damaging	Het
Zbtb10	C	A	3: 9,316,872 (GRCm39)	P228Q	probably damaging	Het
Zfp764l1	G	A	7: 126,991,525 (GRCm39)	T154I	possibly damaging	Het

Other mutations in Trmt10b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01064:Trmt10b	APN	4	45,314,347 (GRCm39)	missense	possibly damaging	0.91
IGL01743:Trmt10b	APN	4	45,305,879 (GRCm39)	missense	probably damaging	1.00
IGL02012:Trmt10b	APN	4	45,315,045 (GRCm39)	missense	probably benign	0.00
R0454:Trmt10b	UTSW	4	45,304,286 (GRCm39)	missense	probably damaging	1.00
R0506:Trmt10b	UTSW	4	45,304,306 (GRCm39)	missense	probably damaging	0.98
R1757:Trmt10b	UTSW	4	45,307,946 (GRCm39)	missense	probably damaging	1.00
R1962:Trmt10b	UTSW	4	45,314,378 (GRCm39)	nonsense	probably null
R2944:Trmt10b	UTSW	4	45,300,445 (GRCm39)	start codon destroyed	probably null	0.99
R4781:Trmt10b	UTSW	4	45,305,817 (GRCm39)	missense	probably damaging	0.98
R5802:Trmt10b	UTSW	4	45,314,236 (GRCm39)	unclassified	probably benign
R6400:Trmt10b	UTSW	4	45,308,562 (GRCm39)	missense	probably damaging	1.00
R6460:Trmt10b	UTSW	4	45,314,322 (GRCm39)	missense	possibly damaging	0.81
R7165:Trmt10b	UTSW	4	45,308,549 (GRCm39)	missense	probably damaging	1.00
R7182:Trmt10b	UTSW	4	45,308,520 (GRCm39)	missense	probably benign	0.36
R8399:Trmt10b	UTSW	4	45,305,870 (GRCm39)	missense	possibly damaging	0.70
R8805:Trmt10b	UTSW	4	45,301,281 (GRCm39)	missense	probably benign	0.31
R9778:Trmt10b	UTSW	4	45,314,374 (GRCm39)	missense	probably damaging	1.00

Posted On

2015-04-16