Incidental Mutation 'IGL02479:Wdr25'
ID295095
Institutional Source Australian Phenomics Network (link to record)
Gene Symbol Wdr25
Ensembl Gene ENSMUSG00000040877
Gene NameWD repeat domain 25
Synonyms
Accession Numbers
Is this an essential gene? Probably essential (E-score: 0.866) question?
Stock #IGL02479
Quality Score
Status
Chromosome12
Chromosomal Location108893631-109028452 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) C to A at 108898601 bp
ZygosityHeterozygous
Amino Acid Change Threonine to Lysine at position 224 (T224K)
Ref Sequence ENSEMBL: ENSMUSP00000129855 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000047115] [ENSMUST00000109848] [ENSMUST00000160477] [ENSMUST00000162748] [ENSMUST00000167816] [ENSMUST00000220495] [ENSMUST00000220667] [ENSMUST00000221377]
Predicted Effect probably benign
Transcript: ENSMUST00000047115
AA Change: T224K

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000035553
Gene: ENSMUSG00000040877
AA Change: T224K

DomainStartEndE-ValueType
low complexity region 120 132 N/A INTRINSIC
low complexity region 180 206 N/A INTRINSIC
WD40 226 268 1.83e-7 SMART
WD40 272 311 6.73e-6 SMART
WD40 312 353 2.58e-1 SMART
Blast:WD40 356 402 7e-11 BLAST
Blast:WD40 407 445 6e-8 BLAST
WD40 451 492 9.6e-2 SMART
WD40 495 535 3e-3 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000109848
SMART Domains Protein: ENSMUSP00000105474
Gene: ENSMUSG00000021266

DomainStartEndE-ValueType
WHEP-TRS 16 72 3.01e-23 SMART
Pfam:tRNA-synt_1b 155 447 5.8e-25 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000160477
Predicted Effect probably benign
Transcript: ENSMUST00000162748
SMART Domains Protein: ENSMUSP00000125102
Gene: ENSMUSG00000021266

DomainStartEndE-ValueType
PDB:2QUK|A 4 37 1e-8 PDB
SCOP:d1fyja_ 14 37 8e-7 SMART
Blast:WHEP 16 37 3e-7 BLAST
Predicted Effect probably benign
Transcript: ENSMUST00000167816
AA Change: T224K

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000129855
Gene: ENSMUSG00000040877
AA Change: T224K

DomainStartEndE-ValueType
low complexity region 120 132 N/A INTRINSIC
low complexity region 180 206 N/A INTRINSIC
WD40 226 268 1.83e-7 SMART
WD40 272 311 6.73e-6 SMART
WD40 312 353 2.58e-1 SMART
Blast:WD40 356 402 7e-11 BLAST
Blast:WD40 407 445 6e-8 BLAST
WD40 451 492 9.6e-2 SMART
WD40 495 535 3e-3 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000220495
Predicted Effect probably benign
Transcript: ENSMUST00000220667
Predicted Effect probably benign
Transcript: ENSMUST00000221377
Coding Region Coverage
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 57 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adam1b T C 5: 121,501,398 Y528C probably damaging Het
Adam24 T A 8: 40,679,532 I13N probably benign Het
Arsj T C 3: 126,438,939 S445P possibly damaging Het
Btf3l4 G A 4: 108,826,176 T31I possibly damaging Het
Cask A T X: 13,557,058 D502E probably damaging Het
Cenpl T A 1: 161,083,067 S195T probably benign Het
Clhc1 T A 11: 29,578,107 I545N probably damaging Het
Clrn2 T C 5: 45,463,912 I216T probably benign Het
Csf2rb C T 15: 78,341,724 Q332* probably null Het
Cyp3a44 T C 5: 145,790,667 D284G probably benign Het
Dgka T C 10: 128,730,246 E345G probably benign Het
Dync1i2 G A 2: 71,235,979 V128I probably damaging Het
Eno3 T A 11: 70,660,888 probably benign Het
Epc2 T A 2: 49,532,135 I347K probably benign Het
F8 A T X: 75,288,240 N681K probably damaging Het
Fam207a T C 10: 77,514,327 S76G probably damaging Het
Fancm T A 12: 65,106,485 D1238E probably damaging Het
Fcna T A 2: 25,625,260 Q237L probably benign Het
Fpr2 A T 17: 17,892,812 R23S probably benign Het
Frmd3 A G 4: 74,187,515 D466G probably benign Het
Gen1 C A 12: 11,241,935 V618L probably benign Het
Gja4 T C 4: 127,312,424 E182G probably benign Het
Gsdmc T A 15: 63,777,975 I356F possibly damaging Het
Kctd19 T C 8: 105,384,768 D102G probably damaging Het
Lrit2 T C 14: 37,072,278 L433P probably damaging Het
Lrp2 G A 2: 69,464,801 probably benign Het
Luc7l3 G A 11: 94,296,909 probably benign Het
Map3k5 T A 10: 20,056,484 L458Q probably damaging Het
Mast4 A G 13: 102,742,037 S1038P probably damaging Het
Med12 A T X: 101,296,992 probably benign Het
Mtor T A 4: 148,470,584 L888M probably damaging Het
Nova1 A T 12: 46,816,918 I83N unknown Het
Obscn A G 11: 59,056,227 probably benign Het
Olfr1309 A C 2: 111,983,385 S230A probably benign Het
Olfr1320 T C X: 49,683,865 V121A probably benign Het
Olfr1426 T A 19: 12,087,905 M296L probably benign Het
Olfr420 C A 1: 174,158,954 Y60* probably null Het
Pde2a A G 7: 101,501,083 Y243C probably damaging Het
Pdzd8 T A 19: 59,299,783 K1062* probably null Het
Phc1 A T 6: 122,323,717 probably benign Het
Pik3c2g T A 6: 139,918,004 S764T probably benign Het
Pmp2 T G 3: 10,182,202 R89S probably benign Het
Prdm2 A G 4: 143,134,929 L597P probably damaging Het
Rfx6 T A 10: 51,678,328 D88E probably benign Het
Rgs9 T C 11: 109,225,652 S442G possibly damaging Het
Ror2 C T 13: 53,131,932 R82Q possibly damaging Het
Sez6 C A 11: 77,978,026 A986E possibly damaging Het
Slc5a5 T A 8: 70,888,911 M325L possibly damaging Het
Sppl2c G A 11: 104,186,937 V188I probably benign Het
Srrm3 G T 5: 135,835,249 C67F probably damaging Het
Stk31 A G 6: 49,421,688 E341G probably damaging Het
Svil A T 18: 5,099,476 M1267L probably damaging Het
Tas2r135 C T 6: 42,405,751 R75* probably null Het
Trav8d-1 T C 14: 52,778,800 S48P probably benign Het
Vrk1 A T 12: 106,051,002 Q95L probably benign Het
Wdr37 A T 13: 8,842,784 H224Q probably damaging Het
Zhx1 C T 15: 58,054,371 E160K probably damaging Het
Other mutations in Wdr25
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00946:Wdr25 APN 12 109025027 missense possibly damaging 0.89
IGL02672:Wdr25 APN 12 108898081 nonsense probably null
IGL03329:Wdr25 APN 12 108898336 missense probably benign
R1061:Wdr25 UTSW 12 108992799 splice site probably null
R1402:Wdr25 UTSW 12 109026539 missense probably damaging 1.00
R1402:Wdr25 UTSW 12 109026539 missense probably damaging 1.00
R1582:Wdr25 UTSW 12 108898054 missense possibly damaging 0.94
R1764:Wdr25 UTSW 12 109026438 nonsense probably null
R1954:Wdr25 UTSW 12 108898541 missense probably damaging 0.99
R2258:Wdr25 UTSW 12 108898174 missense possibly damaging 0.94
R3770:Wdr25 UTSW 12 108898420 missense probably damaging 0.97
R3803:Wdr25 UTSW 12 108898553 missense probably damaging 1.00
R3948:Wdr25 UTSW 12 109027282 missense probably benign 0.02
R4183:Wdr25 UTSW 12 109027331 missense probably benign 0.00
R5246:Wdr25 UTSW 12 109027456 missense probably benign 0.06
R5290:Wdr25 UTSW 12 108898042 missense probably benign 0.26
R5305:Wdr25 UTSW 12 109026440 missense probably damaging 1.00
R5813:Wdr25 UTSW 12 109027421 missense possibly damaging 0.47
R5942:Wdr25 UTSW 12 108898466 missense probably benign 0.00
R6386:Wdr25 UTSW 12 109025065 missense probably damaging 1.00
R7171:Wdr25 UTSW 12 109024996 missense probably damaging 0.98
Posted On2015-04-16