Mutagenetix > Incidental Mutation

Incidental Mutation 'IGL02725:Cry2'

305191

Institutional Source

Australian Phenomics Network (link to record)

Gene Symbol

Cry2

Ensembl Gene

ENSMUSG00000068742

Gene Name

cryptochrome circadian regulator 2

Synonyms

D130054K12Rik

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.579) question?

Stock #

IGL02725

Quality Score

Status

Chromosome

Chromosomal Location

92233991-92264388 bp(-) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

T to C at 92243605 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000106909 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000090559] [ENSMUST00000111278]

AlphaFold

Q9R194

Predicted Effect

probably benign
Transcript: ENSMUST00000090559

SMART Domains

Protein: ENSMUSP00000088047
Gene: ENSMUSG00000068742

Domain	Start	End	E-Value	Type
low complexity region	2	13	N/A	INTRINSIC
Pfam:DNA_photolyase	23	187	2.4e-50	PFAM
Pfam:FAD_binding_7	231	504	4.4e-89	PFAM
low complexity region	562	573	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000111278

SMART Domains

Protein: ENSMUSP00000106909
Gene: ENSMUSG00000068742

Domain	Start	End	E-Value	Type
low complexity region	2	13	N/A	INTRINSIC
Pfam:DNA_photolyase	23	189	3.6e-50	PFAM
Pfam:FAD_binding_7	230	506	1.4e-105	PFAM
low complexity region	562	573	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000126002

Coding Region Coverage

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. [provided by RefSeq, Feb 2014]
PHENOTYPE: Homozygotes for targeted null mutations exhibit a one-hour longer circadian period under constant darkness, and reduced expression of another circadian gene in the suprachiasmatic nucleus in response to acute light exposure. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4833420G17Rik	T	C	13: 119,611,445 (GRCm39)	I414T	possibly damaging	Het
Agpat3	A	C	10: 78,113,889 (GRCm39)	D266E	probably benign	Het
Alpk3	G	T	7: 80,743,358 (GRCm39)	Q1058H	possibly damaging	Het
Atat1	A	T	17: 36,220,381 (GRCm39)	V37E	probably benign	Het
Bcl6b	A	G	11: 70,119,344 (GRCm39)	V124A	probably damaging	Het
Bmt2	T	C	6: 13,628,495 (GRCm39)	E396G	probably damaging	Het
Ccdc50	T	C	16: 27,255,347 (GRCm39)	C237R	probably benign	Het
Chd9	A	G	8: 91,778,312 (GRCm39)	I2790M	possibly damaging	Het
Chkb	A	G	15: 89,313,340 (GRCm39)	L82P	probably damaging	Het
Cit	T	C	5: 116,123,532 (GRCm39)	Y1458H	probably benign	Het
Clp1	T	A	2: 84,554,208 (GRCm39)	K320N	probably benign	Het
Cyp20a1	C	T	1: 60,405,865 (GRCm39)	R220W	probably benign	Het
Cyp27a1	C	T	1: 74,774,862 (GRCm39)	P268S	probably damaging	Het
Cyp2e1	T	G	7: 140,343,828 (GRCm39)	I22S	probably null	Het
Defb34	A	G	8: 19,173,774 (GRCm39)	T3A	unknown	Het
Dpy19l3	A	T	7: 35,411,343 (GRCm39)	M422K	probably benign	Het
Eif4g3	A	T	4: 137,897,782 (GRCm39)		probably benign	Het
Elmod3	A	G	6: 72,571,758 (GRCm39)	S7P	probably damaging	Het
Fam151a	T	G	4: 106,605,211 (GRCm39)	S524R	probably damaging	Het
Gcc1	A	T	6: 28,418,458 (GRCm39)	V625E	probably benign	Het
Glmn	G	A	5: 107,723,155 (GRCm39)	P112S	possibly damaging	Het
Grm5	A	C	7: 87,723,873 (GRCm39)	D721A	probably damaging	Het
Hivep3	T	C	4: 119,953,019 (GRCm39)	V445A	possibly damaging	Het
Hmcn1	T	A	1: 150,480,654 (GRCm39)	E4507D	possibly damaging	Het
Hmcn2	A	C	2: 31,295,540 (GRCm39)	E2583A	probably damaging	Het
Ifnb1	T	A	4: 88,440,867 (GRCm39)	I49F	probably benign	Het
Ints8	A	C	4: 11,239,406 (GRCm39)	C306W	probably benign	Het
Jakmip2	A	G	18: 43,695,655 (GRCm39)	S540P	probably damaging	Het
Kctd1	A	G	18: 15,102,667 (GRCm39)	V838A	possibly damaging	Het
Klk10	T	C	7: 43,431,044 (GRCm39)	L29P	probably damaging	Het
Lyst	T	A	13: 13,935,412 (GRCm39)	I3627K	probably damaging	Het
Maml3	A	G	3: 52,011,195 (GRCm39)	S124P	probably damaging	Het
Nav2	A	T	7: 49,214,843 (GRCm39)	K1632I	probably damaging	Het
Or2ab1	T	C	11: 58,488,690 (GRCm39)	V156A	probably benign	Het
Pde2a	G	T	7: 101,156,425 (GRCm39)	M616I	probably null	Het
Pdia2	T	C	17: 26,415,506 (GRCm39)	D440G	probably benign	Het
Rab11fip5	A	G	6: 85,351,471 (GRCm39)	S14P	probably damaging	Het
Rhpn2	T	C	7: 35,079,031 (GRCm39)	S383P	probably damaging	Het
Rnf220	A	G	4: 117,129,576 (GRCm39)		probably benign	Het
Rnf43	A	G	11: 87,622,411 (GRCm39)	D504G	probably damaging	Het
Setbp1	A	T	18: 78,900,589 (GRCm39)	L1026*	probably null	Het
Slc1a4	A	T	11: 20,258,408 (GRCm39)	S264T	probably damaging	Het
Snx19	A	G	9: 30,343,556 (GRCm39)	N572S	possibly damaging	Het
Sptan1	T	C	2: 29,886,055 (GRCm39)	I739T	probably damaging	Het
Srrm1	G	A	4: 135,052,415 (GRCm39)	P658L	unknown	Het
Stat5b	A	T	11: 100,695,840 (GRCm39)	D47E	possibly damaging	Het
Taar7b	A	T	10: 23,875,961 (GRCm39)	Y42F	probably benign	Het
Tfdp2	T	A	9: 96,169,748 (GRCm39)	I33K	possibly damaging	Het
Tm4sf5	A	G	11: 70,401,448 (GRCm39)	H149R	probably benign	Het
Tmco6	A	G	18: 36,871,760 (GRCm39)	M257V	probably benign	Het
Tuba8	G	T	6: 121,202,916 (GRCm39)	G410*	probably null	Het
Ubd	T	A	17: 37,504,853 (GRCm39)	V4D	probably benign	Het
Ugt2b34	C	T	5: 87,054,284 (GRCm39)	V166I	probably benign	Het
Vmn2r70	C	T	7: 85,214,553 (GRCm39)	V200I	possibly damaging	Het
Wdcp	A	T	12: 4,901,206 (GRCm39)	N354I	probably damaging	Het
Wipi2	A	T	5: 142,652,618 (GRCm39)	Y410F	possibly damaging	Het
Zc3hav1	G	T	6: 38,309,127 (GRCm39)	P565Q	probably damaging	Het
Zfp398	T	C	6: 47,842,737 (GRCm39)	V131A	probably benign	Het
Zkscan3	T	C	13: 21,579,063 (GRCm39)	D144G	possibly damaging	Het
Zpbp	T	A	11: 11,412,358 (GRCm39)		probably benign	Het

Other mutations in Cry2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01995:Cry2	APN	2	92,254,977 (GRCm39)	missense	probably benign	0.15
IGL02167:Cry2	APN	2	92,264,166 (GRCm39)	missense	possibly damaging	0.93
IGL02183:Cry2	APN	2	92,243,384 (GRCm39)	missense	probably damaging	0.99
IGL02343:Cry2	APN	2	92,257,266 (GRCm39)	missense	possibly damaging	0.90
IGL02432:Cry2	APN	2	92,244,012 (GRCm39)	missense	probably damaging	0.99
IGL02932:Cry2	APN	2	92,243,462 (GRCm39)	nonsense	probably null
IGL03122:Cry2	APN	2	92,243,640 (GRCm39)	missense	probably damaging	1.00
IGL03366:Cry2	APN	2	92,244,060 (GRCm39)	missense	probably damaging	1.00
R0679:Cry2	UTSW	2	92,244,060 (GRCm39)	missense	probably damaging	1.00
R1325:Cry2	UTSW	2	92,244,115 (GRCm39)	missense	probably damaging	1.00
R1862:Cry2	UTSW	2	92,254,911 (GRCm39)	missense	probably damaging	1.00
R1891:Cry2	UTSW	2	92,243,985 (GRCm39)	missense	possibly damaging	0.93
R2189:Cry2	UTSW	2	92,242,037 (GRCm39)	missense	possibly damaging	0.84
R4032:Cry2	UTSW	2	92,244,172 (GRCm39)	missense	probably benign	0.00
R4689:Cry2	UTSW	2	92,254,899 (GRCm39)	missense	probably benign	0.38
R5130:Cry2	UTSW	2	92,254,944 (GRCm39)	missense	probably benign	0.28
R5145:Cry2	UTSW	2	92,243,405 (GRCm39)	missense	probably benign
R5970:Cry2	UTSW	2	92,243,312 (GRCm39)	missense	probably benign	0.08
R6179:Cry2	UTSW	2	92,244,187 (GRCm39)	missense	probably damaging	0.98
R7102:Cry2	UTSW	2	92,243,438 (GRCm39)	missense	probably damaging	0.99
R7158:Cry2	UTSW	2	92,244,060 (GRCm39)	missense	probably damaging	1.00
R7213:Cry2	UTSW	2	92,244,004 (GRCm39)	missense	probably benign	0.00
R7257:Cry2	UTSW	2	92,243,326 (GRCm39)	missense	possibly damaging	0.67
R7378:Cry2	UTSW	2	92,244,009 (GRCm39)	missense	probably damaging	1.00
R7427:Cry2	UTSW	2	92,243,392 (GRCm39)	missense	possibly damaging	0.74
R7428:Cry2	UTSW	2	92,243,392 (GRCm39)	missense	possibly damaging	0.74
R7440:Cry2	UTSW	2	92,243,983 (GRCm39)	missense	probably damaging	1.00
R7531:Cry2	UTSW	2	92,243,350 (GRCm39)	missense	probably damaging	0.98
R8234:Cry2	UTSW	2	92,242,974 (GRCm39)	missense	probably benign
R8350:Cry2	UTSW	2	92,244,286 (GRCm39)	missense	probably benign	0.00
R8450:Cry2	UTSW	2	92,244,286 (GRCm39)	missense	probably benign	0.00
R8496:Cry2	UTSW	2	92,257,284 (GRCm39)	missense	probably damaging	1.00
R9172:Cry2	UTSW	2	92,243,993 (GRCm39)	missense	probably damaging	1.00
R9283:Cry2	UTSW	2	92,244,249 (GRCm39)	missense	probably damaging	1.00

Posted On

2015-04-16