Incidental Mutation 'R3925:Zfp953'
ID308253
Institutional Source Beutler Lab
Gene Symbol Zfp953
Ensembl Gene ENSMUSG00000098905
Gene Namezinc finger protein 953
SynonymsE130120F12Rik
MMRRC Submission 040916-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.065) question?
Stock #R3925 (G1)
Quality Score225
Status Validated
Chromosome13
Chromosomal Location67339306-67360605 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to A at 67347938 bp
ZygosityHeterozygous
Amino Acid Change Tyrosine to Phenylalanine at position 13 (Y13F)
Ref Sequence ENSEMBL: ENSMUSP00000049225 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000044819] [ENSMUST00000081582] [ENSMUST00000166080]
Predicted Effect probably damaging
Transcript: ENSMUST00000044819
AA Change: Y13F

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)
SMART Domains Protein: ENSMUSP00000049225
Gene: ENSMUSG00000098781
AA Change: Y13F

DomainStartEndE-ValueType
KRAB 5 65 1.15e-23 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000081582
AA Change: Y13F

PolyPhen 2 Score 0.973 (Sensitivity: 0.76; Specificity: 0.96)
SMART Domains Protein: ENSMUSP00000076700
Gene: ENSMUSG00000098905
AA Change: Y13F

DomainStartEndE-ValueType
KRAB 5 65 1.15e-23 SMART
ZnF_C2H2 81 103 3.11e-2 SMART
ZnF_C2H2 109 131 8.94e-3 SMART
ZnF_C2H2 137 159 6.23e-2 SMART
ZnF_C2H2 165 187 3.63e-3 SMART
ZnF_C2H2 193 215 1.06e-4 SMART
ZnF_C2H2 221 243 7.05e-1 SMART
ZnF_C2H2 249 271 5.42e-2 SMART
ZnF_C2H2 277 299 6.88e-4 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000166080
SMART Domains Protein: ENSMUSP00000126669
Gene: ENSMUSG00000098692

DomainStartEndE-ValueType
KRAB 2 62 3.07e-33 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000170543
Meta Mutation Damage Score 0.0276 question?
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.7%
  • 10x: 97.5%
  • 20x: 95.8%
Validation Efficiency 97% (30/31)
Allele List at MGI
Other mutations in this stock
Total: 31 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adgrv1 G A 13: 81,578,772 T487I possibly damaging Het
Amn T C 12: 111,275,680 V367A possibly damaging Het
C230029F24Rik AGAAAG A 1: 49,310,929 noncoding transcript Het
Cdkl1 G T 12: 69,756,599 R168S probably damaging Het
Cfap43 T C 19: 47,797,116 K445R probably benign Het
Crct1 C A 3: 93,014,707 probably benign Het
Eif4e G A 3: 138,555,437 G164D probably damaging Het
Eno1 T C 4: 150,239,568 probably null Het
Gm38100 T C 1: 175,921,286 V306A probably benign Het
Hmcn2 G A 2: 31,453,157 R4565Q probably benign Het
Itgal A T 7: 127,324,537 probably benign Het
Klhl1 A T 14: 96,346,880 C305S possibly damaging Het
Ms4a2 A G 19: 11,618,948 M139T probably benign Het
Nr2e3 C T 9: 59,948,433 R213H probably damaging Het
Olfr1102 A G 2: 87,002,374 Y135C possibly damaging Het
Olfr224 T A 11: 58,566,826 H173L probably benign Het
Olfr664 T A 7: 104,734,189 E58D probably benign Het
Onecut2 A G 18: 64,341,520 K381E probably damaging Het
Pabpc1l T A 2: 164,027,676 probably benign Het
Prim2 A G 1: 33,533,299 L253P probably damaging Het
Pycr1 T C 11: 120,642,135 T100A probably benign Het
Qrfpr T C 3: 36,221,923 N106S possibly damaging Het
Rsf1 GCG GCGACGGCGACG 7: 97,579,907 probably benign Het
Selenoi T A 5: 30,256,088 D107E probably damaging Het
Synrg C T 11: 84,040,899 P1321S probably benign Het
Tcrg-V7 A G 13: 19,178,474 Y111C probably damaging Het
Trp53rkb A G 2: 166,795,472 Y116C probably damaging Het
Usp34 T A 11: 23,343,640 F245I probably benign Het
Utrn C T 10: 12,698,042 V1095I probably benign Het
Xpnpep1 T C 19: 52,991,697 H632R probably damaging Het
Zfp735 T C 11: 73,711,124 I298T probably benign Het
Other mutations in Zfp953
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL03034:Zfp953 APN 13 67343462 missense probably damaging 1.00
IGL03347:Zfp953 APN 13 67343426 missense probably benign 0.05
R0111:Zfp953 UTSW 13 67343075 missense probably damaging 1.00
R1856:Zfp953 UTSW 13 67345358 missense probably benign 0.02
R2518:Zfp953 UTSW 13 67347939 missense probably damaging 0.99
R4777:Zfp953 UTSW 13 67343129 missense probably benign 0.42
R5647:Zfp953 UTSW 13 67343472 missense possibly damaging 0.90
R6232:Zfp953 UTSW 13 67343097 missense possibly damaging 0.94
R6481:Zfp953 UTSW 13 67347937 nonsense probably null
R7202:Zfp953 UTSW 13 67343642 missense probably benign 0.02
Predicted Primers PCR Primer
(F):5'- CCCCGAGATACTGTACAAAGG -3'
(R):5'- CGTGAAGAATATACATGGTGTGTG -3'

Sequencing Primer
(F):5'- CAAAGGGAGAAACTTTCGTGGTTATC -3'
(R):5'- TCCTAGTCACTGATGGAGCATGAC -3'
Posted On2015-04-17