Incidental Mutation 'R3927:Cog3'
| ID |
308342 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Cog3
|
| Ensembl Gene |
ENSMUSG00000034893 |
| Gene Name |
component of oligomeric golgi complex 3 |
| Synonyms |
|
| MMRRC Submission |
040822-MU
|
| Accession Numbers |
|
| Essential gene? |
Essential
(E-score: 1.000)
|
| Stock # |
R3927 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
14 |
| Chromosomal Location |
75939790-75991998 bp(-) (GRCm39) |
| Type of Mutation |
splice site |
| DNA Base Change (assembly) |
A to G
at 75980998 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000154131
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000049168]
[ENSMUST00000227473]
|
| AlphaFold |
no structure available at present |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000049168
|
| SMART Domains |
Protein: ENSMUSP00000045016
Gene: ENSMUSG00000034893
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
2 |
15 |
N/A |
INTRINSIC |
|
Pfam:Sec34
|
130 |
277 |
9.5e-57 |
PFAM |
|
Blast:HisKA
|
745 |
810 |
1e-5 |
BLAST |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000158780
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000226686
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000227473
|
| Coding Region Coverage |
- 1x: 99.3%
- 3x: 98.7%
- 10x: 97.5%
- 20x: 95.5%
|
| Validation Efficiency |
95% (40/42) |
| MGI Phenotype |
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a component of the conserved oligomeric Golgi (COG) complex which is composed of eight different subunits and is required for normal Golgi morphology and localization. Defects in the COG complex result in multiple deficiencies in protein glycosylation. The protein encoded by this gene is involved in ER-Golgi transport.[provided by RefSeq, Jun 2011]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 37 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Abtb2 |
A |
G |
2: 103,538,563 (GRCm39) |
|
probably null |
Het |
| Alpk1 |
T |
C |
3: 127,471,365 (GRCm39) |
H1039R |
probably damaging |
Het |
| Avpr1a |
A |
G |
10: 122,285,616 (GRCm39) |
S303G |
probably benign |
Het |
| Axdnd1 |
A |
G |
1: 156,246,840 (GRCm39) |
L79S |
probably damaging |
Het |
| Baz1a |
A |
G |
12: 54,967,928 (GRCm39) |
I667T |
possibly damaging |
Het |
| Bend5 |
A |
G |
4: 111,305,802 (GRCm39) |
Y282C |
possibly damaging |
Het |
| Clstn3 |
T |
C |
6: 124,428,327 (GRCm39) |
D438G |
probably damaging |
Het |
| Cyp2j6 |
T |
C |
4: 96,441,525 (GRCm39) |
N55S |
probably benign |
Het |
| Eif4b |
G |
A |
15: 101,992,745 (GRCm39) |
G101R |
probably damaging |
Het |
| Epha2 |
T |
C |
4: 141,033,861 (GRCm39) |
L40P |
probably damaging |
Het |
| Fig4 |
A |
G |
10: 41,139,135 (GRCm39) |
V356A |
probably benign |
Het |
| Hal |
T |
C |
10: 93,349,888 (GRCm39) |
|
probably benign |
Het |
| Helz |
A |
G |
11: 107,576,118 (GRCm39) |
Y1770C |
unknown |
Het |
| Meis3 |
A |
G |
7: 15,911,419 (GRCm39) |
T39A |
probably benign |
Het |
| Nod1 |
G |
T |
6: 54,921,902 (GRCm39) |
R139S |
probably benign |
Het |
| Or6n2 |
A |
T |
1: 173,896,878 (GRCm39) |
N5Y |
probably damaging |
Het |
| Pacsin3 |
C |
T |
2: 91,093,286 (GRCm39) |
|
probably null |
Het |
| Plekhh1 |
T |
A |
12: 79,100,422 (GRCm39) |
I130N |
probably damaging |
Het |
| Plxna2 |
G |
A |
1: 194,428,465 (GRCm39) |
E512K |
probably benign |
Het |
| Ppp1r9a |
A |
G |
6: 5,057,531 (GRCm39) |
I215M |
probably damaging |
Het |
| Ryr3 |
C |
G |
2: 112,506,218 (GRCm39) |
R3443P |
probably damaging |
Het |
| Sap130 |
A |
T |
18: 31,807,435 (GRCm39) |
H414L |
possibly damaging |
Het |
| Slc33a1 |
A |
G |
3: 63,871,145 (GRCm39) |
I156T |
probably benign |
Het |
| Slc37a2 |
G |
A |
9: 37,146,803 (GRCm39) |
T338M |
probably damaging |
Het |
| Spinkl |
T |
A |
18: 44,302,230 (GRCm39) |
|
probably null |
Het |
| Tmc5 |
T |
A |
7: 118,251,878 (GRCm39) |
L657* |
probably null |
Het |
| Tmem217 |
A |
G |
17: 29,745,677 (GRCm39) |
S18P |
probably damaging |
Het |
| Trim43a |
GATTTATTTATTTATTTATTTATTTATTTATTTATTTATT |
GATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATT |
9: 88,465,042 (GRCm39) |
|
probably benign |
Het |
| Tubb4a |
A |
G |
17: 57,387,967 (GRCm39) |
V353A |
probably benign |
Het |
| Ube3b |
T |
C |
5: 114,553,741 (GRCm39) |
F974L |
probably benign |
Het |
| Ubqln5 |
A |
G |
7: 103,777,678 (GRCm39) |
L382P |
probably damaging |
Het |
| Ufsp2 |
T |
A |
8: 46,436,723 (GRCm39) |
|
probably null |
Het |
| Unkl |
C |
T |
17: 25,448,303 (GRCm39) |
T66I |
probably damaging |
Het |
| Xrn2 |
T |
A |
2: 146,880,109 (GRCm39) |
N477K |
probably benign |
Het |
| Zfhx4 |
C |
T |
3: 5,468,418 (GRCm39) |
P2859S |
probably benign |
Het |
| Zzef1 |
T |
C |
11: 72,749,208 (GRCm39) |
S899P |
probably damaging |
Het |
| Zzz3 |
T |
C |
3: 152,161,499 (GRCm39) |
Y298H |
probably damaging |
Het |
|
| Other mutations in Cog3 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL02019:Cog3
|
APN |
14 |
75,968,044 (GRCm39) |
missense |
possibly damaging |
0.79 |
|
IGL02637:Cog3
|
APN |
14 |
75,959,636 (GRCm39) |
splice site |
probably benign |
|
|
IGL02934:Cog3
|
APN |
14 |
75,979,129 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R0105:Cog3
|
UTSW |
14 |
75,959,580 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R0105:Cog3
|
UTSW |
14 |
75,959,580 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R0403:Cog3
|
UTSW |
14 |
75,979,767 (GRCm39) |
splice site |
probably benign |
|
|
R0972:Cog3
|
UTSW |
14 |
75,954,610 (GRCm39) |
missense |
probably benign |
|
|
R1735:Cog3
|
UTSW |
14 |
75,966,761 (GRCm39) |
nonsense |
probably null |
|
|
R1813:Cog3
|
UTSW |
14 |
75,979,784 (GRCm39) |
missense |
probably benign |
0.03 |
|
R1896:Cog3
|
UTSW |
14 |
75,979,784 (GRCm39) |
missense |
probably benign |
0.03 |
|
R2517:Cog3
|
UTSW |
14 |
75,979,182 (GRCm39) |
missense |
probably benign |
0.01 |
|
R2567:Cog3
|
UTSW |
14 |
75,991,730 (GRCm39) |
missense |
probably benign |
|
|
R2962:Cog3
|
UTSW |
14 |
75,977,974 (GRCm39) |
critical splice donor site |
probably null |
|
|
R3103:Cog3
|
UTSW |
14 |
75,984,641 (GRCm39) |
critical splice acceptor site |
probably null |
|
|
R3689:Cog3
|
UTSW |
14 |
75,991,878 (GRCm39) |
start codon destroyed |
probably null |
|
|
R3691:Cog3
|
UTSW |
14 |
75,991,878 (GRCm39) |
start codon destroyed |
probably null |
|
|
R4581:Cog3
|
UTSW |
14 |
75,970,391 (GRCm39) |
missense |
probably benign |
0.04 |
|
R4932:Cog3
|
UTSW |
14 |
75,970,394 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R5560:Cog3
|
UTSW |
14 |
75,966,833 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5654:Cog3
|
UTSW |
14 |
75,962,239 (GRCm39) |
missense |
probably benign |
0.03 |
|
R6253:Cog3
|
UTSW |
14 |
75,957,152 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6419:Cog3
|
UTSW |
14 |
75,962,178 (GRCm39) |
nonsense |
probably null |
|
|
R6791:Cog3
|
UTSW |
14 |
75,968,118 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6803:Cog3
|
UTSW |
14 |
75,941,479 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7015:Cog3
|
UTSW |
14 |
75,950,716 (GRCm39) |
missense |
possibly damaging |
0.81 |
|
R7998:Cog3
|
UTSW |
14 |
75,984,533 (GRCm39) |
missense |
possibly damaging |
0.94 |
|
R7999:Cog3
|
UTSW |
14 |
75,984,533 (GRCm39) |
missense |
possibly damaging |
0.94 |
|
R8075:Cog3
|
UTSW |
14 |
75,968,142 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8294:Cog3
|
UTSW |
14 |
75,954,619 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8329:Cog3
|
UTSW |
14 |
75,978,003 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8434:Cog3
|
UTSW |
14 |
75,979,836 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9170:Cog3
|
UTSW |
14 |
75,966,802 (GRCm39) |
missense |
probably damaging |
1.00 |
|
X0017:Cog3
|
UTSW |
14 |
75,979,181 (GRCm39) |
missense |
probably benign |
0.01 |
|
X0021:Cog3
|
UTSW |
14 |
75,981,033 (GRCm39) |
missense |
possibly damaging |
0.87 |
|
X0066:Cog3
|
UTSW |
14 |
75,979,181 (GRCm39) |
missense |
probably benign |
0.01 |
|
| Predicted Primers |
PCR Primer
(F):5'- CCAAGCATGCTTCTCCTGAAC -3'
(R):5'- GCTGAGCCCTAGAAATAAGATTCAG -3'
Sequencing Primer
(F):5'- TGCTTCTCCTGAACAATAAATACCTC -3'
(R):5'- GCCCTAGAAATAAGATTCAGTTAGG -3'
|
| Posted On |
2015-04-17 |
Incidental Mutation