Mutagenetix > Incidental Mutation

Incidental Mutation 'R3929:Tars3'

308635

Institutional Source

Beutler Lab

Gene Symbol

Tars3

Ensembl Gene

ENSMUSG00000030515

Gene Name

threonyl-tRNA synthetase 3

Synonyms

A530046H20Rik, Tarsl2

MMRRC Submission

040824-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.178) question?

Stock #

R3929 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

65294646-65341839 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

A to G at 65333791 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000032728 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032728]

AlphaFold

Q8BLY2

Predicted Effect

probably null
Transcript: ENSMUST00000032728

SMART Domains

Protein: ENSMUSP00000032728
Gene: ENSMUSG00000030515

Domain	Start	End	E-Value	Type
low complexity region	2	18	N/A	INTRINSIC
coiled coil region	44	68	N/A	INTRINSIC
Pfam:TGS	151	210	8.8e-14	PFAM
tRNA_SAD	316	365	1.26e-16	SMART
Pfam:tRNA-synt_2b	464	675	2.2e-35	PFAM
Pfam:HGTP_anticodon	687	778	1.1e-20	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000126941

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127354

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.5%
20x: 95.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 33 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ak5	A	C	3: 152,373,444 (GRCm39)	L18R	probably damaging	Het
Atrx	T	C	X: 104,923,523 (GRCm39)	I157V	possibly damaging	Het
C9	T	A	15: 6,496,939 (GRCm39)	I212N	probably benign	Het
Cabin1	T	C	10: 75,587,452 (GRCm39)		probably null	Het
Ccnf	A	G	17: 24,453,356 (GRCm39)	V361A	probably damaging	Het
Ctps1	T	A	4: 120,399,093 (GRCm39)	H553L	probably benign	Het
Dmrta1	C	T	4: 89,579,681 (GRCm39)	Q214*	probably null	Het
E230025N22Rik	T	C	18: 36,824,625 (GRCm39)	D112G	probably damaging	Het
Frat1	T	C	19: 41,819,087 (GRCm39)	C161R	probably damaging	Het
H1f8	A	G	6: 115,925,757 (GRCm39)	K185E	probably benign	Het
Itpr2	C	A	6: 146,275,857 (GRCm39)		probably null	Het
Klhl40	A	G	9: 121,609,742 (GRCm39)	D509G	probably benign	Het
Muc5ac	T	C	7: 141,356,629 (GRCm39)	V1072A	probably benign	Het
Nav3	A	G	10: 109,520,064 (GRCm39)	Y2340H	probably damaging	Het
Or10ak9	T	C	4: 118,726,179 (GRCm39)	L66P	probably damaging	Het
Or2y17	A	T	11: 49,231,820 (GRCm39)	M154L	probably benign	Het
Or51a43	C	T	7: 103,717,791 (GRCm39)	C149Y	probably benign	Het
Or5be3	T	A	2: 86,864,428 (GRCm39)	I46F	possibly damaging	Het
Or6c2	G	A	10: 129,362,100 (GRCm39)	M1I	probably null	Het
Prdm10	T	C	9: 31,258,432 (GRCm39)	I619T	probably damaging	Het
Rp1	T	C	1: 4,422,868 (GRCm39)	T71A	probably damaging	Het
Rpusd4	A	G	9: 35,183,876 (GRCm39)	I202V	probably benign	Het
Sin3a	A	G	9: 57,025,421 (GRCm39)	N1089S	probably damaging	Het
St6gal2	A	G	17: 55,803,324 (GRCm39)	D353G	possibly damaging	Het
Stap2	A	C	17: 56,310,156 (GRCm39)	F50V	probably damaging	Het
Stkld1	T	A	2: 26,830,059 (GRCm39)		probably null	Het
Tbl1xr1	G	C	3: 22,243,932 (GRCm39)	D69H	probably damaging	Het
Tnrc6c	C	T	11: 117,614,355 (GRCm39)	R838W	probably damaging	Het
Trim61	A	G	8: 65,465,969 (GRCm39)	F431L	probably benign	Het
Trmo	C	T	4: 46,382,647 (GRCm39)	G150S	probably damaging	Het
Vmn1r61	A	G	7: 5,614,176 (GRCm39)	I46T	probably benign	Het
Vmn2r19	T	C	6: 123,292,587 (GRCm39)	Y210H	probably benign	Het
Xrn1	T	C	9: 95,870,926 (GRCm39)	S584P	possibly damaging	Het

Other mutations in Tars3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00092:Tars3	APN	7	65,302,007 (GRCm39)	critical splice acceptor site	probably null
IGL00470:Tars3	APN	7	65,338,656 (GRCm39)	missense	probably benign	0.03
IGL00594:Tars3	APN	7	65,325,880 (GRCm39)	critical splice donor site	probably null
IGL01352:Tars3	APN	7	65,308,658 (GRCm39)	missense	possibly damaging	0.80
IGL01519:Tars3	APN	7	65,313,634 (GRCm39)	missense	probably damaging	1.00
IGL01726:Tars3	APN	7	65,332,566 (GRCm39)	missense	possibly damaging	0.46
IGL02370:Tars3	APN	7	65,310,913 (GRCm39)	missense	probably benign	0.17
IGL02729:Tars3	APN	7	65,332,567 (GRCm39)	missense	probably damaging	0.97
IGL03234:Tars3	APN	7	65,302,026 (GRCm39)	missense	probably benign	0.06
gary	UTSW	7	65,338,700 (GRCm39)	critical splice donor site	probably null
R8254_tarsl2_650	UTSW	7	65,325,809 (GRCm39)	missense	probably benign
smart_money	UTSW	7	65,327,890 (GRCm39)	missense	probably damaging	1.00
R0127:Tars3	UTSW	7	65,314,717 (GRCm39)	missense	probably benign	0.19
R0153:Tars3	UTSW	7	65,333,829 (GRCm39)	missense	probably damaging	1.00
R0605:Tars3	UTSW	7	65,327,819 (GRCm39)	missense	probably damaging	1.00
R1070:Tars3	UTSW	7	65,305,444 (GRCm39)	missense	probably damaging	1.00
R1450:Tars3	UTSW	7	65,297,244 (GRCm39)	missense	probably benign	0.01
R1467:Tars3	UTSW	7	65,305,444 (GRCm39)	missense	probably damaging	1.00
R1467:Tars3	UTSW	7	65,305,444 (GRCm39)	missense	probably damaging	1.00
R2142:Tars3	UTSW	7	65,308,645 (GRCm39)	missense	probably benign
R2143:Tars3	UTSW	7	65,305,539 (GRCm39)	missense	possibly damaging	0.57
R2144:Tars3	UTSW	7	65,305,539 (GRCm39)	missense	possibly damaging	0.57
R2145:Tars3	UTSW	7	65,305,539 (GRCm39)	missense	possibly damaging	0.57
R2208:Tars3	UTSW	7	65,332,596 (GRCm39)	missense	probably damaging	1.00
R3713:Tars3	UTSW	7	65,338,700 (GRCm39)	critical splice donor site	probably null
R3715:Tars3	UTSW	7	65,338,700 (GRCm39)	critical splice donor site	probably null
R3914:Tars3	UTSW	7	65,333,556 (GRCm39)	missense	probably benign	0.05
R4008:Tars3	UTSW	7	65,327,876 (GRCm39)	missense	probably damaging	1.00
R4064:Tars3	UTSW	7	65,302,018 (GRCm39)	missense	possibly damaging	0.90
R4367:Tars3	UTSW	7	65,332,567 (GRCm39)	missense	probably damaging	0.97
R4652:Tars3	UTSW	7	65,339,717 (GRCm39)	missense	probably damaging	1.00
R4825:Tars3	UTSW	7	65,297,302 (GRCm39)	missense	probably benign	0.38
R4901:Tars3	UTSW	7	65,341,042 (GRCm39)	missense	probably benign	0.05
R4999:Tars3	UTSW	7	65,308,683 (GRCm39)	missense	probably damaging	0.99
R5423:Tars3	UTSW	7	65,333,567 (GRCm39)	missense	probably benign	0.00
R5756:Tars3	UTSW	7	65,325,724 (GRCm39)	missense	probably benign	0.22
R5772:Tars3	UTSW	7	65,333,873 (GRCm39)	missense	probably damaging	1.00
R6160:Tars3	UTSW	7	65,332,527 (GRCm39)	missense	probably benign	0.32
R6230:Tars3	UTSW	7	65,336,184 (GRCm39)	splice site	probably null
R6424:Tars3	UTSW	7	65,305,487 (GRCm39)	missense	probably damaging	1.00
R6615:Tars3	UTSW	7	65,327,890 (GRCm39)	missense	probably damaging	1.00
R6792:Tars3	UTSW	7	65,312,051 (GRCm39)	missense	probably damaging	1.00
R7350:Tars3	UTSW	7	65,308,672 (GRCm39)	missense	probably damaging	1.00
R7549:Tars3	UTSW	7	65,297,341 (GRCm39)	missense	probably damaging	0.96
R7592:Tars3	UTSW	7	65,308,619 (GRCm39)	missense	probably benign	0.01
R7634:Tars3	UTSW	7	65,325,760 (GRCm39)	missense	probably damaging	0.99
R7710:Tars3	UTSW	7	65,314,717 (GRCm39)	missense	probably benign	0.19
R7808:Tars3	UTSW	7	65,302,009 (GRCm39)	missense	probably benign	0.01
R7875:Tars3	UTSW	7	65,327,899 (GRCm39)	missense	probably benign	0.05
R8254:Tars3	UTSW	7	65,325,809 (GRCm39)	missense	probably benign
R8793:Tars3	UTSW	7	65,294,673 (GRCm39)	start gained	probably benign
R9162:Tars3	UTSW	7	65,332,518 (GRCm39)	missense	probably benign	0.01
R9200:Tars3	UTSW	7	65,302,013 (GRCm39)	missense	probably benign
R9461:Tars3	UTSW	7	65,339,719 (GRCm39)	missense	possibly damaging	0.68
R9533:Tars3	UTSW	7	65,333,808 (GRCm39)	critical splice acceptor site	probably null
Z1177:Tars3	UTSW	7	65,302,012 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AGAACAGCTTGGTGGAATTTGG -3'
(R):5'- TTGTATTGCACGTGATCTTACGAC -3'

Sequencing Primer
(F):5'- AGATGGAGCATTCTATGGCCC -3'
(R):5'- GCACGTGATCTTACGACAAATAATG -3'

Posted On

2015-04-17