Incidental Mutation 'R3901:Casp16'
ID 308995
Institutional Source Beutler Lab
Gene Symbol Casp16
Ensembl Gene ENSMUSG00000067882
Gene Name caspase 16, apoptosis-related cysteine peptidase
Synonyms Casp16-ps, Gm5146, Casp16
MMRRC Submission 040810-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.058) question?
Stock # R3901 (G1)
Quality Score 225
Status Validated
Chromosome 17
Chromosomal Location 23770054-23774304 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 23771922 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Valine to Glutamic Acid at position 101 (V101E)
Ref Sequence ENSEMBL: ENSMUSP00000086048 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000088673] [ENSMUST00000095606] [ENSMUST00000182769]
AlphaFold I6L890
Predicted Effect probably damaging
Transcript: ENSMUST00000088673
AA Change: V101E

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000086048
Gene: ENSMUSG00000067882
AA Change: V101E

DomainStartEndE-ValueType
Pfam:Peptidase_C14 3 162 4.5e-9 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000095606
SMART Domains Protein: ENSMUSP00000093266
Gene: ENSMUSG00000071256

DomainStartEndE-ValueType
SCAN 41 145 3.11e-56 SMART
KRAB 213 278 3.21e-4 SMART
ZnF_C2H2 326 348 3.89e-3 SMART
ZnF_C2H2 354 376 9.88e-5 SMART
ZnF_C2H2 382 404 6.42e-4 SMART
ZnF_C2H2 410 432 1.95e-3 SMART
ZnF_C2H2 438 460 4.4e-2 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000180920
Predicted Effect probably benign
Transcript: ENSMUST00000182769
SMART Domains Protein: ENSMUSP00000138283
Gene: ENSMUSG00000067882

DomainStartEndE-ValueType
Pfam:Peptidase_C14 18 175 1.7e-7 PFAM
Meta Mutation Damage Score 0.6467 question?
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.7%
  • 10x: 97.5%
  • 20x: 95.7%
Validation Efficiency 98% (39/40)
Allele List at MGI
Other mutations in this stock
Total: 39 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adamts20 T A 15: 94,226,726 (GRCm39) D1109V possibly damaging Het
Apc2 G C 10: 80,150,922 (GRCm39) R1992P possibly damaging Het
Atmin A G 8: 117,683,036 (GRCm39) N232S probably benign Het
Brat1 A C 5: 140,703,751 (GRCm39) D668A possibly damaging Het
Cass4 C T 2: 172,274,478 (GRCm39) P753L probably damaging Het
Cimip3 AC A 17: 47,744,348 (GRCm39) probably benign Het
Clec7a A G 6: 129,445,877 (GRCm39) S98P possibly damaging Het
Cpne5 G A 17: 29,378,082 (GRCm39) R566C unknown Het
Csrnp1 C T 9: 119,801,707 (GRCm39) E451K probably damaging Het
Dlst A G 12: 85,179,465 (GRCm39) T435A possibly damaging Het
Dock8 A G 19: 25,078,269 (GRCm39) T525A possibly damaging Het
Dync2li1 A G 17: 84,939,070 (GRCm39) T45A probably damaging Het
Efcab3 A G 11: 104,974,713 (GRCm39) N5307S possibly damaging Het
Epha4 A G 1: 77,357,539 (GRCm39) Y820H probably damaging Het
F5 A G 1: 164,003,798 (GRCm39) T198A probably benign Het
Fbn2 T A 18: 58,199,083 (GRCm39) N1395I probably damaging Het
Fcnb T A 2: 27,969,208 (GRCm39) Y163F probably damaging Het
Gm5444 T C 13: 4,884,278 (GRCm39) noncoding transcript Het
Jph3 G T 8: 122,480,158 (GRCm39) D279Y possibly damaging Het
Kcnj3 A G 2: 55,327,360 (GRCm39) N50D possibly damaging Het
Kcnma1 A G 14: 23,555,323 (GRCm39) I416T probably damaging Het
Kirrel1 C T 3: 86,996,458 (GRCm39) M380I probably null Het
Kmt2e A G 5: 23,706,640 (GRCm39) N1401S probably benign Het
Lama5 C T 2: 179,824,144 (GRCm39) probably benign Het
Lrp1b A G 2: 40,712,707 (GRCm39) V3095A probably damaging Het
Mmp1a A G 9: 7,475,346 (GRCm39) *372W probably null Het
Or7d11 C T 9: 19,966,169 (GRCm39) V197I probably benign Het
Pdgfra T A 5: 75,353,169 (GRCm39) N986K probably benign Het
Pgap1 C T 1: 54,532,507 (GRCm39) V671I probably benign Het
Pla2g4e T C 2: 119,999,085 (GRCm39) S760G probably benign Het
Plk3 T C 4: 116,990,633 (GRCm39) I94V probably benign Het
Pogk C T 1: 166,231,193 (GRCm39) V45I probably damaging Het
Pou2f1 G C 1: 165,722,538 (GRCm39) P349R probably damaging Het
Rims1 T A 1: 22,572,578 (GRCm39) Q541L probably benign Het
Rptn A G 3: 93,305,664 (GRCm39) Q999R probably benign Het
Stxbp5 A G 10: 9,645,163 (GRCm39) L911P probably damaging Het
Tgfbr3 A G 5: 107,362,753 (GRCm39) probably benign Het
Trim7 A T 11: 48,728,435 (GRCm39) T28S probably damaging Het
Zfp595 T C 13: 67,465,379 (GRCm39) I295V probably benign Het
Other mutations in Casp16
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01767:Casp16 APN 17 23,771,027 (GRCm39) missense probably damaging 1.00
R0316:Casp16 UTSW 17 23,771,066 (GRCm39) missense probably damaging 1.00
R1717:Casp16 UTSW 17 23,771,024 (GRCm39) missense possibly damaging 0.93
R6848:Casp16 UTSW 17 23,770,053 (GRCm39) nonsense probably null
R8358:Casp16 UTSW 17 23,772,348 (GRCm39) missense probably damaging 0.98
R9133:Casp16 UTSW 17 23,771,003 (GRCm39) missense probably damaging 1.00
R9158:Casp16 UTSW 17 23,769,948 (GRCm39) unclassified probably benign
RF008:Casp16 UTSW 17 23,774,201 (GRCm39) unclassified probably benign
Predicted Primers PCR Primer
(F):5'- TGTGGAGCTGAAAACCCACAAG -3'
(R):5'- AAAGGTATCTGCCCCATCCTTC -3'

Sequencing Primer
(F):5'- GCATCCAGGCTGTATGAACTCAG -3'
(R):5'- ATCCTTCTCCAGGGAACAGG -3'
Posted On 2015-04-17